Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,358,482 T→A I46F (ATC→TTC)  cadA ← lysine decarboxylase, acid‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,358,4820TA96.1% 420.8 / 2.2 129I46F (ATC→TTC) cadAlysine decarboxylase, acid‑inducible
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base A (64/59);  total (68/61)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 129 total aligned reads displayed.

GCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTTCTTCTTTAAA  >  NC_000913/4358386‑4358578
                                                                                                |                                                                                                
gCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAg                                                                                               <  2:1197858/100‑1 (MQ=255)
gCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAg                                                                                               <  2:85004/100‑1 (MQ=255)
   ggTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAGATAACGCCGCACAGACGCGCATTGTTTTCGAACAGttt                                                                                            >  1:1891540/1‑100 (MQ=255)
   ggTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGttt                                                                                            <  1:337469/100‑1 (MQ=255)
   ggTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGttt                                                                                            >  1:2656759/1‑100 (MQ=255)
   ggTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGttt                                                                                            >  2:1143981/1‑100 (MQ=255)
    gTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGtttt                                                                                           <  2:1488894/100‑1 (MQ=255)
    gTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGtttt                                                                                           <  2:2097170/100‑1 (MQ=255)
     ttCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGAGTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTta                                                                                          >  2:1739754/1‑100 (MQ=255)
      tctcGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTtaa                                                                                         >  2:1312571/1‑100 (MQ=255)
       ctcGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTtaat                                                                                        <  1:818923/100‑1 (MQ=255)
         cGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTtaataa                                                                                      >  1:1349805/1‑100 (MQ=255)
         cGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTtaataa                                                                                      >  1:1564686/1‑100 (MQ=255)
         cGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTtaataa                                                                                      >  1:2041575/1‑100 (MQ=255)
          gTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAg                                                                                     >  1:84515/1‑100 (MQ=255)
          gTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAg                                                                                     >  2:3100161/1‑100 (MQ=255)
            tCATTTTGCTAATTTCTTCGCACAGCTCGAGATGATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAgtc                                                                                   >  1:2097103/1‑100 (MQ=255)
              aTTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAgtcgt                                                                                 >  2:2993308/1‑100 (MQ=255)
                tttGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCa                                                                               >  2:2847155/1‑100 (MQ=255)
                   gCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACgg                                                                            <  2:1973654/100‑1 (MQ=255)
                    cTAATTTCTTCTCACAGCTCTATATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTTTTTTCGAACAGTTTTAATAAGTCGTCACGGt                                                                           <  1:1227949/100‑1 (MQ=255)
                    cTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTTAAAAATAACGCCACACAGACGCGCATTGTTTTCGAACAGATATAATAAGTCGACACGGt                                                                           >  1:2423925/1‑100 (MQ=255)
                    cTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGt                                                                           >  1:3143354/1‑100 (MQ=255)
                     tAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTc                                                                          >  2:441524/1‑100 (MQ=255)
                     tAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTc                                                                          <  2:2837727/100‑1 (MQ=255)
                     ccgatctttcGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTc                                                                          <  2:2655908/93‑1 (MQ=255)
                        tttATTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGtt                                                                       >  1:2746758/1‑100 (MQ=255)
                         ttcttcGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTc                                                                      >  1:1242430/1‑100 (MQ=255)
                          tcttcGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCg                                                                     <  1:748665/100‑1 (MQ=255)
                           cttcGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCgg                                                                    >  1:3421594/1‑100 (MQ=255)
                           cttcGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCgg                                                                    >  1:3275101/1‑100 (MQ=255)
                            ttcGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTcagatcgg                                                                   >  1:2655870/1‑93 (MQ=255)
                            ttcGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCggg                                                                   <  2:1294733/100‑1 (MQ=255)
                              cGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTa                                                                 <  2:359107/100‑1 (MQ=255)
                               gCACAGCTCGATATTATATTTATCCCAGTCAAAGATAATGACGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTTACGGTCGTTCGGGt                                                                  <  1:801265/98‑1 (MQ=255)
                               gCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTaa                                                                >  2:2152895/1‑100 (MQ=255)
                               gCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTaa                                                                >  2:1839240/1‑100 (MQ=255)
                                cacaGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTaaa                                                               >  1:478361/1‑100 (MQ=255)
                                 acaGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAAc                                                              <  2:524460/100‑1 (MQ=255)
                                   aGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACaa                                                            >  1:136277/1‑100 (MQ=255)
                                    gCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAAt                                                           >  1:723596/1‑100 (MQ=255)
                                     cTCGAGATTATATTTATCCCTGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATc                                                          >  1:2129434/1‑100 (MQ=255)
                                     cTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATc                                                          <  1:617965/100‑1 (MQ=255)
                                      tCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCt                                                         <  2:1803413/100‑1 (MQ=255)
                                      tCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCt                                                         >  1:469890/1‑100 (MQ=255)
                                       cGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTg                                                        <  2:1598749/100‑1 (MQ=255)
                                       cGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTg                                                        >  2:3457873/1‑100 (MQ=255)
                                         agaTTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGa                                                      <  2:1619814/100‑1 (MQ=255)
                                         agaTTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGa                                                      >  2:1226528/1‑100 (MQ=255)
                                           aTTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAg                                                    <  2:525790/100‑1 (MQ=255)
                                           aTTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAg                                                    <  2:1938932/100‑1 (MQ=255)
                                           aTTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAg                                                    <  2:2436624/100‑1 (MQ=255)
                                           aTTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAg                                                    <  2:3231179/100‑1 (MQ=255)
                                            ttATATTTATCCCAGTCAAAAATAACGCCGCACAGAGGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACagatcggaaga                                                   >  2:2444832/1‑90 (MQ=255)
                                              atatTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTc                                                 <  2:1930157/100‑1 (MQ=255)
                                                atTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAg                                               >  1:829680/1‑100 (MQ=255)
                                                 tttATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGa                                              >  2:769047/1‑100 (MQ=255)
                                                   tATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACg                                            >  2:863112/1‑100 (MQ=255)
                                                    aTCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGt                                           >  1:942528/1‑100 (MQ=255)
                                                      cccAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTc                                         >  1:440781/1‑100 (MQ=255)
                                                      cccAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTc                                         >  2:1427761/1‑100 (MQ=255)
                                                      cccAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTc                                         >  1:1194637/1‑100 (MQ=255)
                                                       ccAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCa                                        <  1:2465408/100‑1 (MQ=255)
                                                         aGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAg                                      <  1:1086663/100‑1 (MQ=255)
                                                         aGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAg                                      <  1:2880410/100‑1 (MQ=255)
                                                          gTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAgc                                     >  2:1841338/1‑100 (MQ=255)
                                                          gTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAgc                                     >  2:329144/1‑100 (MQ=255)
                                                           tCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAgcg                                    <  1:1143966/100‑1 (MQ=255)
                                                            cAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAgcgc                                   >  1:2243661/1‑100 (MQ=255)
                                                            cAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAgcgc                                   >  2:2715361/1‑100 (MQ=255)
                                                            cAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAgcgc                                   >  2:3041842/1‑100 (MQ=255)
                                                            cAAAAATAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAgcgc                                   >  2:2572780/1‑100 (MQ=255)
                                                             aaaaaTAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACCTTCAAgcgcg                                  >  2:3099850/1‑100 (MQ=255)
                                                               aaaTAACGCCGCACAGACGCGCATTGTTTTCGACCAGTTTTACTAAGTCGACACAGTCGTTCCGGTAAACAATCTTGAAGTTCAGACGTTCatacccgcg                                <  1:2598454/100‑9 (MQ=255)
                                                                 aTAACGCCGCACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGAt                              <  1:1275756/100‑1 (MQ=255)
                                                                     cgccgcACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAg                          >  2:3360491/1‑100 (MQ=255)
                                                                     cgccgcACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAg                          <  2:136279/100‑1 (MQ=255)
                                                                     cgccgcACAGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAg                          >  2:2533064/1‑100 (MQ=255)
                                                                           acaGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACg                    <  1:1739729/100‑1 (MQ=255)
                                                                            caGACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACgg                   <  1:3065127/100‑1 (MQ=255)
                                                                              gtggcgcATTGGTTTCGAACAGTTTTAATAAGTCTTCACGGTCGGTAGGGTAAACAATCTGGAAGTTCAGACGTTCAAGAGCGCGATGAAGTTCACGGAt                 <  1:1561158/97‑1 (MQ=255)
                                                                              gACGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGAt                 >  1:1085113/1‑100 (MQ=255)
                                                                               aCGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATg                <  2:354320/100‑1 (MQ=255)
                                                                               aCGCGCATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATg                <  1:2459562/100‑1 (MQ=255)
                                                                                  cgcATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGt             >  2:2473506/1‑100 (MQ=255)
                                                                                   gcATTGTTTTCTAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGTAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATggg              <  1:3470664/98‑1 (MQ=255)
                                                                                   gcATTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGtt            >  2:2171126/1‑100 (MQ=255)
                                                                                     aTTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGttct          >  2:2331495/1‑100 (MQ=255)
                                                                                     aTTGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGttct          <  2:3246832/100‑1 (MQ=255)
                                                                                      ttGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGttctt         <  1:2418095/100‑1 (MQ=255)
                                                                                       tGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGttcttc        <  2:3001975/100‑1 (MQ=255)
                                                                                       tGTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGttcttc        <  1:2355074/100‑1 (MQ=255)
                                                                                        gTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTtcttct       >  1:1028118/1‑100 (MQ=255)
                                                                                        gTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTtcttct       <  2:1564708/100‑1 (MQ=255)
                                                                                        gTTTTCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTtcttct       >  1:3050041/1‑100 (MQ=255)
                                                                                         ttttCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTTcttctt      >  1:996538/1‑100 (MQ=255)
                                                                                         ttttCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTTcttctt      >  1:291848/1‑100 (MQ=255)
                                                                                           ttCGAACAGTTTTTTTAAGTCGTTACGGTGGTGTGGGTAAACAATCTGGAAGGTCAGACGTTTAAGCGCGTGATGAAGTGAACGGATGGGTACTTCTTTa    <  1:1019299/100‑1 (MQ=255)
                                                                                            tCGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTTCTTCTTTaa   <  1:2871453/100‑1 (MQ=255)
                                                                                             cGAACAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTTCTTCTTTaaa  >  1:2273480/1‑100 (MQ=255)
                                                                                                |                                                                                                
GCAGGTTCTCGTTCATTTTGCTAATTTCTTCGCACAGCTCGAGATTATATTTATCCCAGTCAAAAATAACGCCGCACAGACGCGCATTGTTTTCGATCAGTTTTAATAAGTCGTCACGGTCGTTCGGGTAAACAATCTGGAAGTTCAGACGTTCAAGCGCGCGATGAAGTTCACGGATGGGTTCTTCTTTAAA  >  NC_000913/4358386‑4358578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: