Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,360,814 T→G Y374S (TAC→TCC)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,360,8140TG98.5% 447.8 / NA 133Y374S (TAC→TCC) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (60/70);  total (63/70)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTG  >  NC_000913/4360717‑4360907
                                                                                                 |                                                                                             
gTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAg                                                                                             <  2:1491441/100‑1 (MQ=255)
  tATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGt                                                                                           >  1:879733/1‑100 (MQ=255)
    tCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGt                                                                                           >  1:784436/1‑98 (MQ=255)
     cTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaaa                                                                                        <  2:2111969/100‑1 (MQ=255)
     cTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaaa                                                                                        >  2:1173785/1‑100 (MQ=255)
       aTTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAtt                                                                                      >  2:2973046/1‑100 (MQ=255)
       aTTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAtt                                                                                      >  2:1502839/1‑100 (MQ=255)
          tCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTg                                                                                   <  1:1024348/100‑1 (MQ=255)
           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                  <  2:869791/100‑1 (MQ=255)
           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                  <  2:2395147/100‑1 (MQ=255)
           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                  <  1:837095/100‑1 (MQ=255)
           cTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgg                                                                                  <  1:2986213/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                 <  2:879745/100‑1 (MQ=255)
             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                <  1:2421072/100‑1 (MQ=255)
                 tCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAGTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgatga                                                                            >  1:2952635/1‑100 (MQ=255)
                  cAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGgatgat                                                                           >  1:1050049/1‑100 (MQ=255)
                    gTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTg                                                                         <  1:2384730/100‑1 (MQ=255)
                    gTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTCTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTg                                                                         <  1:1856752/100‑1 (MQ=255)
                       ctgctAATTGTTTTTCATCTCAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGaa                                                                       >  1:2122254/1‑99 (MQ=255)
                       ctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAAc                                                                      <  1:3177395/100‑1 (MQ=255)
                        tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                     <  1:310123/100‑1 (MQ=255)
                        tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                     >  2:1814981/1‑100 (MQ=255)
                        tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                     >  2:2742445/1‑100 (MQ=255)
                        tgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                     >  2:2742600/1‑100 (MQ=255)
                        tgctAACTGTTTTTCATCTAAAGTATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACa                                                                     <  1:3287276/100‑1 (MQ=255)
                         gctAATTGTTTTTCATCTAACGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACaa                                                                    >  1:616740/1‑100 (MQ=255)
                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtc                                                                  <  1:130856/100‑1 (MQ=255)
                            aaTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTTTTTTTTTTTGGAGGTAAATTCTGGGGATGATTGAACAAtct                                                                 >  1:2652695/1‑100 (MQ=255)
                            aaTTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtct                                                                 >  2:2539245/1‑100 (MQ=255)
                            aaTTGTTCTTCATCTAAAGGACGTTGCGAATGGCGCACGATATCACCTAATGCTTTTTCTCCTCTCGCGCAGGAAAATTCTGGGGATGATCGAACaa                                                                    >  1:2858421/1‑97 (MQ=255)
                              ttGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCa                                                               <  2:450536/100‑1 (MQ=255)
                               tGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGTTAAATTCTGGGGATGATTGAACAATCTCAc                                                              <  1:1705989/100‑1 (MQ=255)
                               tGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAc                                                              >  2:3239264/1‑100 (MQ=255)
                                gTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTTCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAcc                                                             <  2:3447985/100‑1 (MQ=255)
                                gTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCAcc                                                             <  2:1153698/100‑1 (MQ=255)
                                   tttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTAGCGTAGGTAAATTCTGGGGATGGTTGAACAATCTCACCtaa                                                          >  2:2623157/1‑100 (MQ=255)
                                     tCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaata                                                        <  1:2498699/100‑1 (MQ=255)
                                     tCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaata                                                        >  2:2077798/1‑100 (MQ=255)
                                       aTCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTaataat                                                      <  2:2735071/100‑1 (MQ=255)
                                        tCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAAtt                                                     >  2:643654/1‑100 (MQ=255)
                                         cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                    <  2:1440724/100‑1 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAAtt                                                     >  1:1880539/1‑98 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                   >  1:1765600/1‑100 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGAAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                   >  1:2059913/1‑100 (MQ=255)
                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                  <  2:1687153/100‑1 (MQ=255)
                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                  >  1:2919059/1‑100 (MQ=255)
                                             aGGATGTTGAGAATGGCGCACGATATCAACTAAGGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTg                                                <  2:3264297/100‑1 (MQ=255)
                                              ggATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                               <  2:1462232/100‑1 (MQ=255)
                                              cgatcttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTgg                                               <  1:2358100/95‑1 (MQ=255)
                                                aTGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCa                                             >  1:1760577/1‑100 (MQ=255)
                                                 tGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAc                                            >  2:1133364/1‑100 (MQ=255)
                                                 tGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAc                                            >  1:234420/1‑100 (MQ=255)
                                                  gTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACg                                           >  2:847159/1‑100 (MQ=255)
                                                   ttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACgg                                          <  2:2692767/100‑1 (MQ=255)
                                                   ttGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACgg                                          >  2:2086884/1‑100 (MQ=255)
                                                    tcagaATGGCGCAAGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTggagatcg                                         >  2:2358132/3‑94 (MQ=255)
                                                    tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                         <  2:2947831/100‑1 (MQ=255)
                                                    tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                         >  1:3298015/1‑100 (MQ=255)
                                                     gagaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTAACCTAATAATTCACTGGCACGGtt                                        >  1:3420802/1‑100 (MQ=255)
                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                       <  2:3152272/100‑1 (MQ=255)
                                                        aaaggCGCAGGATATCAACTAATGCTAGTTCTACAATAGCCGAGGCAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTCGCACGGtt                                        <  1:1895599/94‑1 (MQ=255)
                                                        aaTGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCaa                                     <  1:2587845/100‑1 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                   <  2:3253080/100‑1 (MQ=255)
                                                          tGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATg                                   <  1:23259/100‑1 (MQ=255)
                                                             cgcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAtt                                <  1:1606098/100‑1 (MQ=255)
                                                              gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                               >  2:3237246/1‑100 (MQ=255)
                                                              gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                               <  1:206972/100‑1 (MQ=255)
                                                               cACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGTGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                               >  1:1389696/1‑99 (MQ=255)
                                                               cACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTa                              <  1:1404816/100‑1 (MQ=255)
                                                               cACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTa                              >  1:1674704/1‑100 (MQ=255)
                                                                atgATAGCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCTCCTAATAATTCACTGGCACGGTTCAATGATTTat                             <  2:1543017/98‑1 (MQ=255)
                                                                aCGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTat                             <  1:1617777/100‑1 (MQ=255)
                                                                aCGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTat                             >  2:1454086/1‑100 (MQ=255)
                                                                        aaCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCa                     <  1:37207/100‑1 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                  <  1:53621/100‑1 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                  <  2:866264/100‑1 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                  <  1:3390185/100‑1 (MQ=255)
                                                                            aaTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCa                 >  1:1622016/1‑100 (MQ=255)
                                                                             aTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAg                <  1:2934555/100‑1 (MQ=255)
                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCGTGCAGTa              >  2:2988745/1‑100 (MQ=255)
                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa              >  2:703001/1‑100 (MQ=255)
                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTa              <  1:1122976/100‑1 (MQ=255)
                                                                               gCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATACAGTa              >  1:490795/1‑100 (MQ=255)
                                                                                cTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaa             >  1:396593/1‑100 (MQ=255)
                                                                                 tttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGaaaa            >  2:450047/1‑100 (MQ=255)
                                                                                 gatctCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaa            <  2:2311157/96‑1 (MQ=255)
                                                                                  ttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaat           <  2:1878989/100‑1 (MQ=255)
                                                                                     tCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaataat        <  1:2989708/100‑1 (MQ=255)
                                                                                     tCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaataat        <  2:38557/100‑1 (MQ=255)
                                                                                      cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaaagatc       >  1:2311125/1‑95 (MQ=255)
                                                                                      cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc       <  2:2652733/100‑1 (MQ=255)
                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa      >  1:2689756/1‑100 (MQ=255)
                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAt     >  1:2299276/1‑100 (MQ=255)
                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAt     <  2:2703457/100‑1 (MQ=255)
                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAt     <  1:368658/100‑1 (MQ=255)
                                                                                         ctctCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtg    >  1:1684405/1‑100 (MQ=255)
                                                                                         ctctCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtg    >  1:1815059/1‑100 (MQ=255)
                                                                                          tctcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTATTAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtgt   >  2:2497596/1‑100 (MQ=255)
                                                                                           ctcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtgtg  >  1:3376653/1‑100 (MQ=255)
                                                                                            tcGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtgtg  <  1:1122194/99‑1 (MQ=255)
                                                                                                 |                                                                                             
GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTG  >  NC_000913/4360717‑4360907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: