Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 53,012 A→G G135G (GGT→GGC)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091353,0120AG98.3% 409.0 / ‑5.1 121G135G (GGT→GGC) pdxA4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (58/60);  total (61/60)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 121 total aligned reads displayed.

GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACG  >  NC_000913/52915‑53106
                                                                                                 |                                                                                              
gTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGcc                                                                                              <  2:102614/100‑1 (MQ=255)
 tAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCAGCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg                                                                                             >  1:1074242/1‑100 (MQ=255)
 tAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg                                                                                             <  2:802830/100‑1 (MQ=255)
 tAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg                                                                                             <  2:1528232/100‑1 (MQ=255)
       cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaaaa                                                                                       >  1:2305590/1‑100 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                      <  2:2187739/100‑1 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                      <  2:1676736/100‑1 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                      <  2:1672024/100‑1 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                      <  2:1653608/100‑1 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                      <  2:299699/100‑1 (MQ=255)
         tcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAgg                                                                                     <  2:1931272/100‑1 (MQ=255)
            ttGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAAt                                                                                  <  2:2014554/100‑1 (MQ=255)
             tGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATg                                                                                 <  2:312159/100‑1 (MQ=255)
               ccAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGcc                                                                               <  1:1472013/100‑1 (MQ=255)
               ccAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGcc                                                                               <  1:301343/100‑1 (MQ=255)
                 aGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAg                                                                             >  2:1970544/1‑100 (MQ=255)
                      cACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcg                                                                        >  2:155447/1‑100 (MQ=255)
                      cACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcg                                                                        >  2:977054/1‑100 (MQ=255)
                       aCGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcgt                                                                       >  1:1476548/1‑100 (MQ=255)
                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcgt                                                                       >  1:2312615/1‑99 (MQ=255)
                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGtt                                                                      <  1:2914447/100‑1 (MQ=255)
                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGtt                                                                      <  1:499518/100‑1 (MQ=255)
                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGtt                                                                      >  2:3312831/1‑100 (MQ=255)
                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAAAGAAATAAAAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGtt                                                                      <  1:1784634/100‑1 (MQ=255)
                         gcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTta                                                                     <  1:920064/100‑1 (MQ=255)
                         gcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTta                                                                     <  2:2832037/100‑1 (MQ=255)
                          cgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTtaa                                                                    >  2:2634449/1‑100 (MQ=255)
                            aaGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGACGGTAAAAGGAATGCCAGCGTCGTtaata                                                                  >  2:560120/1‑100 (MQ=255)
                              gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAAc                                                                <  2:2801049/100‑1 (MQ=255)
                              gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTAGCCTGCGAACGCTCTTCGAAAAACTAGGTATGGTCGGTAAAAGGAATGCCAGCGTCGTTAATAAc                                                                >  1:1449962/1‑100 (MQ=255)
                                tcttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGc                                                              >  2:1791626/1‑100 (MQ=255)
                                 cttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGcc                                                             <  1:668735/100‑1 (MQ=255)
                                   tcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAACGAATGCCAGCGTCGTTAATAACGCCtt                                                           >  1:1270719/1‑100 (MQ=255)
                                    cGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGAAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                          >  1:1934575/1‑100 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                         >  2:1731090/1‑100 (MQ=255)
                                      gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTAt                                                        <  1:1536683/100‑1 (MQ=255)
                                      gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCATCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTAt                                                        >  1:1899052/1‑100 (MQ=255)
                                       tCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGAAAAAGCAATGCCAGCGCCGTTAATAACGCCTTTATg                                                       >  1:1790406/1‑100 (MQ=255)
                                        cccccggatcatcACCCCTTTTTTCGCCCGCGAACGCTCTTCAAAAAACTCGGTATCCCCGGTAAAAAAAATTCCAGCGTCGTTAATAACCCCttt                                                          >  1:445254/8‑96 (MQ=255)
                                        cGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                      >  2:661195/1‑100 (MQ=255)
                                           cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                   <  2:925708/100‑1 (MQ=255)
                                           cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                   >  2:1076400/1‑100 (MQ=255)
                                           cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                   >  1:2762866/1‑100 (MQ=255)
                                            aGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCg                                                  >  2:2583008/1‑100 (MQ=255)
                                               atcatcACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAc                                               <  1:376617/100‑1 (MQ=255)
                                               atcatcACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAc                                               >  1:1715979/1‑100 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTTTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                              <  2:3040732/100‑1 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                              >  1:298968/1‑100 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                              >  2:764016/1‑100 (MQ=255)
                                                 catcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt                                             >  2:1634251/1‑100 (MQ=255)
                                                  atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtg                                            >  2:2457721/1‑100 (MQ=255)
                                                  atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtg                                            <  1:972354/100‑1 (MQ=255)
                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTAGTTAATAACGCCTTTATGCACCGGACCtg                                            >  1:1394141/1‑99 (MQ=255)
                                                    caccacCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg                                          >  1:2869524/1‑100 (MQ=255)
                                                    caccacCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgtg                                          >  1:3286101/1‑100 (MQ=255)
                                                     accaccTTTTTCTCCTTCTTTTTCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGa                                         >  1:451685/1‑100 (MQ=255)
                                                     accaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGa                                         <  2:1865724/100‑1 (MQ=255)
                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                        <  2:824694/100‑1 (MQ=255)
                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                        >  1:567143/1‑100 (MQ=255)
                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAGCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                       <  2:437589/100‑1 (MQ=255)
                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAGCGCCTTTATGCACCGGACCTGTGATc                                       <  1:1024933/100‑1 (MQ=255)
                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                       >  2:1602841/1‑100 (MQ=255)
                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                       <  2:2737682/100‑1 (MQ=255)
                                                        accTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCa                                      <  2:178236/100‑1 (MQ=255)
                                                        accTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCa                                      <  1:2818553/100‑1 (MQ=255)
                                                         ccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAg                                     >  2:358790/1‑100 (MQ=255)
                                                             tttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGcgcg                                 >  1:123995/1‑100 (MQ=255)
                                                              cttccgatctaaCGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                <  2:79224/90‑1 (MQ=255)
                                                               tCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGc                               <  2:2398976/100‑1 (MQ=255)
                                                                cGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGAACCGGACCTGTGATCAGCGCGGc                               >  1:2643295/1‑99 (MQ=255)
                                                                 gtcTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCaa                             <  1:2111624/98‑1 (MQ=255)
                                                                   cTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAAt                           <  2:2479558/100‑1 (MQ=255)
                                                                    tGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAAtt                          >  2:3138921/1‑100 (MQ=255)
                                                                    tGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAAtt                          >  2:2684197/1‑100 (MQ=255)
                                                                    tGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAAtt                          <  2:3053143/100‑1 (MQ=255)
                                                                     gCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                         >  2:1061666/1‑100 (MQ=255)
                                                                     gCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                         <  1:598400/100‑1 (MQ=255)
                                                                      cGAACGCTCTTCGAAAAGCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                         <  1:155445/99‑1 (MQ=255)
                                                                      cGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACTGGACCTGTGATCAGCGCGGCaa                             >  1:3368152/1‑95 (MQ=255)
                                                                       gAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGc                       >  2:11534/1‑100 (MQ=255)
                                                                        aaCGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCggagatcggaag                      >  1:79223/1‑90 (MQ=255)
                                                                         atcttctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCg                     <  2:1307946/96‑1 (MQ=255)
                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGt                    <  2:2297025/98‑1 (MQ=255)
                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                  <  1:3411717/100‑1 (MQ=255)
                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                  <  1:2992485/100‑1 (MQ=255)
                                                                             tctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCgagat                 >  1:1307927/1‑96 (MQ=255)
                                                                              ctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                >  1:2014216/1‑100 (MQ=255)
                                                                              ctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                >  2:3437605/1‑100 (MQ=255)
                                                                              ctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                >  2:1714717/1‑100 (MQ=255)
                                                                              ctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                <  2:1970293/100‑1 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              >  1:2602147/1‑100 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              >  1:2542432/1‑100 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              >  1:3262168/1‑100 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              <  1:2023876/100‑1 (MQ=255)
                                                                                 cGAAAAACTCGGTATGGCCGGTAAAAGGAATGTCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACa             <  2:304350/100‑1 (MQ=255)
                                                                                    aaaaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAcc          <  1:1288704/100‑1 (MQ=255)
                                                                                    aaaaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAcc          >  2:1430152/1‑100 (MQ=255)
                                                                                      aaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCAt        <  1:3162074/100‑1 (MQ=255)
                                                                                         tCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGc     >  1:2992993/1‑100 (MQ=255)
                                                                                            gTATGGCCGGTAAAAGGAATGACAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACg  >  1:86066/1‑100 (MQ=255)
                                                                                                 |                                                                                              
GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACG  >  NC_000913/52915‑53106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑