BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	364,024	C→T	R761H (CGC→CAC)	lacZ ←	beta‑D‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,024	0	C	T	97.3%	470.9 / ‑1.0	149	R761H (CGC→CAC)	lacZ	beta‑D‑galactosidase
Reads supporting (aligned to +/- strand): ref base C (3/1); new base T (73/72); total (76/73)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

GTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTC  >  NC_000913/363927‑364119

gTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACtggtgg                                                                                               <  1:190541/100‑1 (MQ=255)

  cGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGtt                                                                                             >  2:597505/1‑100 (MQ=255)

   gTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTa                                                                                            >  2:1559249/1‑100 (MQ=255)

     tATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTaaa                                                                                          <  1:3302946/100‑1 (MQ=255)

      aTCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAt                                                                                         >  1:621628/1‑100 (MQ=255)

      aTCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAt                                                                                         <  1:2251696/100‑1 (MQ=255)

       tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                        <  1:733270/100‑1 (MQ=255)

       tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                        <  1:1847125/100‑1 (MQ=255)

        ccAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTg                                                                                       <  2:2916879/100‑1 (MQ=255)

        ccAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTg                                                                                       <  2:1354646/100‑1 (MQ=255)

        ccAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTg                                                                                       >  2:2842696/1‑100 (MQ=255)

         ctgCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                      >  2:3041976/3‑100 (MQ=255)

         cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAACGCCTGACTGGTGGTTAAATTGc                                                                                      >  1:3045449/1‑100 (MQ=255)

         cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                      >  1:1953263/1‑100 (MQ=255)

         cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                      >  2:2807176/1‑100 (MQ=255)

         cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                      <  1:492207/100‑1 (MQ=255)

         cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                      >  2:498156/1‑100 (MQ=255)

         cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGc                                                                                      <  1:2563546/100‑1 (MQ=255)

               tGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGc                                                                                <  1:3376004/100‑1 (MQ=255)

                   cGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttat                                                                            >  2:1831074/1‑100 (MQ=255)

                    gggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatt                                                                           >  2:3340124/1‑100 (MQ=255)

                     ggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCttatta                                                                          >  2:2056619/1‑100 (MQ=255)

                      gTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAc                                                                         >  1:132579/1‑100 (MQ=255)

                       tGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTAcc                                                                        <  2:854156/100‑1 (MQ=255)

                         aaCTGATCGCGCAGCGGCGACAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCa                                                                      <  1:2776095/100‑1 (MQ=255)

                          aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAg                                                                     <  2:3302177/100‑1 (MQ=255)

                             gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                  >  1:1074735/1‑100 (MQ=255)

                             gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCATGACTGGTGGTTAAATTGCCAACGATTATTCACCAGCt                                                                   >  2:2377731/1‑99 (MQ=255)

                              aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCg                                                                 >  1:1062163/1‑100 (MQ=255)

                              aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCg                                                                 <  2:3059835/100‑1 (MQ=255)

                               tCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGa                                                                <  1:380482/100‑1 (MQ=255)

                               tCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGa                                                                <  1:616917/100‑1 (MQ=255)

                                cgcgCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGAt                                                               >  2:1953392/1‑100 (MQ=255)

                                  cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGc                                                             >  2:2317999/1‑100 (MQ=255)

                                  cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGc                                                             <  2:3004772/100‑1 (MQ=255)

                                   gcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCa                                                            >  1:596987/1‑100 (MQ=255)

                                      gcggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCaaaa                                                         <  1:3470665/100‑1 (MQ=255)

                                        ggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGTCTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAAt                                                       >  1:2821063/1‑100 (MQ=255)

                                         gcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATc                                                      <  2:1912914/100‑1 (MQ=255)

                                          cgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATcc                                                     <  1:1812357/100‑1 (MQ=255)

                                              agcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                 >  2:2478417/1‑100 (MQ=255)

                                              agcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                 <  1:3216662/100‑1 (MQ=255)

                                              agcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAACCCAtt                                                  <  2:461761/99‑1 (MQ=255)

                                               gcagTTGTTTTTTATCGCCAATCCACATCTTTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                                <  1:3397031/100‑1 (MQ=255)

                                               gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                                <  2:3205095/100‑1 (MQ=255)

                                                  gttgttTTTTATTTTCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCt                                             >  1:95007/1‑100 (MQ=255)

                                                  gttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACACTTATTACCCAGCTCGATGCAAAAATCCATTTCGCt                                             >  1:2641240/1‑100 (MQ=255)

                                                   ttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtg                                            <  2:607040/100‑1 (MQ=255)

                                                    tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtgg                                           <  1:2747925/100‑1 (MQ=255)

                                                    tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtgg                                           <  2:2724378/100‑1 (MQ=255)

                                                    tgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtgg                                           >  2:2388875/1‑100 (MQ=255)

                                                     gttTTTTCTCGCCAAACCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCtggt                                          <  1:3141928/100‑1 (MQ=255)

                                                     gttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggt                                          >  1:1330636/1‑100 (MQ=255)

                                                     gttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggt                                          <  1:1246683/100‑1 (MQ=255)

                                                     gttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggt                                          >  2:1246699/1‑100 (MQ=255)

                                                       tttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggtgg                                        <  1:2450792/100‑1 (MQ=255)

                                                        ttttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTggtggt                                       >  2:3182564/1‑100 (MQ=255)

                                                         tttATCGCCAATACACATCTGTGAAAGAAACCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATCCAAAAACACATTTCGCtggagtca                                      >  1:2711135/1‑95 (MQ=255)

                                                          ttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCa                                     <  2:893226/100‑1 (MQ=255)

                                                          ttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCa                                     >  2:1185618/1‑100 (MQ=255)

                                                          ttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCa                                     <  1:889753/100‑1 (MQ=255)

                                                           tATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                    <  1:2676404/100‑1 (MQ=255)

                                                              cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATg                                 >  2:3142002/1‑100 (MQ=255)

                                                              cGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATg                                 >  2:334830/1‑100 (MQ=255)

                                                                  aaTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                             <  2:737329/100‑1 (MQ=255)

                                                                  aaTCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                             <  2:1089490/100‑1 (MQ=255)

                                                                    tCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGAt                           >  2:1777031/1‑100 (MQ=255)

                                                                      cacaTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATgg                         <  1:1814007/100‑1 (MQ=255)

                                                                        caTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGagatcg                       >  1:1842417/1‑95 (MQ=255)

                                                                         aTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGCCAGATGCGGGATgg                         >  1:1727399/1‑97 (MQ=255)

                                                                          tCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                     >  1:2046345/1‑100 (MQ=255)

                                                                           cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                    <  2:1410219/100‑1 (MQ=255)

                                                                           cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                    <  2:2247272/100‑1 (MQ=255)

                                                                           cTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTgg                    >  2:443077/1‑100 (MQ=255)

                                                                            tgtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTggg                   >  2:535151/1‑100 (MQ=255)

                                                                             gtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                  >  1:829385/1‑100 (MQ=255)

                                                                             gtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                  >  2:1388200/1‑100 (MQ=255)

                                                                             gtgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                  >  2:1699740/1‑100 (MQ=255)

                                                                              tgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAc                 <  2:1228778/100‑1 (MQ=255)

                                                                              tgAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAc                 >  2:1438844/1‑100 (MQ=255)

                                                                               gaaagaaaGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg                >  2:2748744/1‑100 (MQ=255)

                                                                                aaagaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc               <  1:3421185/100‑1 (MQ=255)

                                                                                aaagaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc               <  2:3286636/100‑1 (MQ=255)

                                                                                aaagaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc               >  1:2255363/1‑100 (MQ=255)

                                                                                aaagaaagCATGACTGGTAGTTGAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgc               >  1:2026137/1‑100 (MQ=255)

                                                                                  agaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACgcgg             <  1:404398/100‑1 (MQ=255)

                                                                                   gaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGTGAcgctcc            >  2:1348389/1‑97 (MQ=255)

                                                                                   gaaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCACTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACgcggc            <  2:1135458/100‑1 (MQ=255)

                                                                                    aaagCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACgcggcg           <  1:1169231/100‑1 (MQ=255)

                                                                                       gCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCgggg        <  1:597498/100‑1 (MQ=255)

                                                                                       gCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCgggg        <  2:2071270/100‑1 (MQ=255)

                                                                                       gCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCgggg        >  2:1771832/1‑100 (MQ=255)

                                                                                        ccTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGa       >  2:2520410/1‑100 (MQ=255)

                                                                                           naCTGGTGGTTAAATTGCCANCGCTTATTACCCAGCTCGATGCAAAAATNCATTTCGATGGTGGTCAGATGCGNGANGGCGNGGNACGCGGCGGGGcgcg    >  1:646921/2‑100 (MQ=255)

                                                                                           gACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg    <  1:452193/100‑1 (MQ=255)

                                                                                           gACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg    >  1:937996/1‑100 (MQ=255)

                                                                                           gACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg    >  2:640225/1‑100 (MQ=255)

                                                                                            aCTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGt   <  2:1279605/100‑1 (MQ=255)

                                                                                             cTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTc  <  2:2776029/100‑1 (MQ=255)

                                                                                             cTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTc  <  1:1176695/100‑1 (MQ=255)

GTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTC  >  NC_000913/363927‑364119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`