Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 367,830 G→A Y197Y (TAC→TAT mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913367,8300GA99.1% 368.0 / ‑4.9 111Y197Y (TAC→TATmhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (69/41);  total (69/42)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 111 total aligned reads displayed.

CCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTC  >  NC_000913/367733‑367925
                                                                                                 |                                                                                               
ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATa                                                                                               >  1:2124329/1‑100 (MQ=255)
ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATa                                                                                               >  2:289231/1‑100 (MQ=255)
  aGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAAc                                                                                             >  2:1102400/1‑100 (MQ=255)
   gATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAAcc                                                                                            <  1:3060993/100‑1 (MQ=255)
    aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAACCGCGGTAGTTCTGTCCATAAccc                                                                                           >  2:1453614/1‑100 (MQ=255)
     ttCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCct                                                                                          >  2:859054/1‑100 (MQ=255)
     ttCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCct                                                                                          >  2:1322051/1‑100 (MQ=255)
       cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGCTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCctct                                                                                        >  1:2411543/1‑100 (MQ=255)
       cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCctct                                                                                        >  1:152133/1‑100 (MQ=255)
       cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCctct                                                                                        >  2:476294/1‑100 (MQ=255)
        aGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCtt                                                                                       >  2:2643634/1‑100 (MQ=255)
         gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCttt                                                                                      <  2:1566840/100‑1 (MQ=255)
         gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCttt                                                                                      >  2:2312413/1‑100 (MQ=255)
          aCAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTg                                                                                     >  1:3217826/1‑100 (MQ=255)
           cAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgc                                                                                    <  2:1187463/100‑1 (MQ=255)
            aGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcg                                                                                   >  2:1177370/1‑100 (MQ=255)
             gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgc                                                                                  >  2:1661255/1‑100 (MQ=255)
             gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgc                                                                                  >  2:1043105/1‑100 (MQ=255)
               cAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcg                                                                                 <  1:3032096/99‑1 (MQ=255)
                aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcg                                                                               <  1:1911036/100‑1 (MQ=255)
                aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcg                                                                               >  2:1099410/1‑100 (MQ=255)
                    aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcgcg                                                                           >  2:3250785/1‑100 (MQ=255)
                     cccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcgcgc                                                                          >  1:704133/1‑100 (MQ=255)
                      ccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGcc                                                                         >  2:2518381/1‑100 (MQ=255)
                        gttgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAg                                                                       >  2:1134959/1‑100 (MQ=255)
                           gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAAt                                                                    >  2:119948/1‑100 (MQ=255)
                            ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATg                                                                   <  2:1849278/100‑1 (MQ=255)
                            ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATg                                                                   >  2:2321585/1‑100 (MQ=255)
                            ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATg                                                                   >  2:2563315/1‑100 (MQ=255)
                              cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  1:1000594/1‑100 (MQ=255)
                              cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  2:701808/1‑100 (MQ=255)
                              cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  2:3339220/1‑100 (MQ=255)
                              cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                 >  1:2742784/1‑100 (MQ=255)
                                 tGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTc                                                              >  2:1269034/1‑100 (MQ=255)
                                  gcgcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTcc                                                             <  2:2837516/100‑1 (MQ=255)
                                      aGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCt                                                         <  2:888413/100‑1 (MQ=255)
                                      aGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCt                                                         <  1:1075303/100‑1 (MQ=255)
                                       gCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCtt                                                        <  1:2273295/100‑1 (MQ=255)
                                        cGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCttt                                                       <  1:2321553/100‑1 (MQ=255)
                                        cGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCttt                                                       <  1:1164396/100‑1 (MQ=255)
                                          gTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTaa                                                     <  1:2518348/100‑1 (MQ=255)
                                           tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                    <  1:1062858/100‑1 (MQ=255)
                                           tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                    <  1:1175688/100‑1 (MQ=255)
                                             cGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCACGCGCGCCAGAATGGCTTCCAGCTTTAACgg                                                  >  2:2177966/1‑100 (MQ=255)
                                              ggCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGt                                                 >  1:2146859/1‑100 (MQ=255)
                                               gCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGtt                                                >  1:892661/1‑100 (MQ=255)
                                               gCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGtt                                                >  2:1660894/1‑100 (MQ=255)
                                               gCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGtt                                                <  2:2962293/100‑1 (MQ=255)
                                                cGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACAGTTc                                               <  1:2491021/100‑1 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          <  1:2602098/100‑1 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          <  2:409585/100‑1 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          >  1:1407432/1‑100 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          >  1:3355442/1‑100 (MQ=255)
                                                     gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                          >  1:953375/1‑100 (MQ=255)
                                                      agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  2:1088675/1‑100 (MQ=255)
                                                       ggCGATGTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        <  2:3165801/100‑1 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGCATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  1:2122657/1‑99 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        >  1:373754/1‑100 (MQ=255)
                                                       ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                         >  1:345803/1‑99 (MQ=255)
                                                         cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                      >  2:2035651/1‑100 (MQ=255)
                                                         cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                      >  1:1190813/1‑100 (MQ=255)
                                                         cGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAg                                      >  2:359042/1‑100 (MQ=255)
                                                          gaccttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCCTGCCAg                                      >  1:758911/4‑99 (MQ=255)
                                                          gATCTTCTCCTCCTGATCCCAGCCGCTGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCTCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                        <  1:3200154/97‑1 (MQ=255)
                                                           aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                    <  1:3245021/100‑1 (MQ=255)
                                                           aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                    <  2:2237880/100‑1 (MQ=255)
                                                           aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                    >  2:1986005/1‑100 (MQ=255)
                                                            tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTg                                   <  1:289227/100‑1 (MQ=255)
                                                             cttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                  >  1:2645832/1‑100 (MQ=255)
                                                             cttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                  <  2:2140344/100‑1 (MQ=255)
                                                              ttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGAt                                 <  2:1830785/100‑1 (MQ=255)
                                                               tgtcctccTGATCCGCGCCGCGGTAGTTCTGTCCAGATCCCTCTTTGCGCGCCCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTCCCTGTTGAt                                 <  1:178900/97‑1 (MQ=255)
                                                               tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                <  2:1234289/100‑1 (MQ=255)
                                                                ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAg                               >  1:3469081/1‑100 (MQ=255)
                                                                ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAg                               <  2:452381/100‑1 (MQ=255)
                                                                ctcctcCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAg                               <  2:1102055/100‑1 (MQ=255)
                                                                   ctcctGATCCCAGCCGCGGTAGTTGTGGCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGGGCCAGTTGATAGtc                             <  1:2825724/99‑1 (MQ=255)
                                                                   ctcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtca                            <  1:3215491/100‑1 (MQ=255)
                                                                    tcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcat                           >  1:3313728/1‑100 (MQ=255)
                                                                     cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                          >  1:3037779/1‑100 (MQ=255)
                                                                      ctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                         >  2:1789267/1‑100 (MQ=255)
                                                                      ctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatca                         >  2:1669676/1‑100 (MQ=255)
                                                                           cccAGCCGCGGTAGTTCTGTCCATAACGCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTGCCCGTGCCAGTTGATAGTCATCACCggg                    >  2:1025691/1‑100 (MQ=255)
                                                                           cccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCggg                    >  2:1137477/1‑100 (MQ=255)
                                                                                ccGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCggg               <  2:1439364/100‑1 (MQ=255)
                                                                                 cgcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCggg               >  1:1470351/1‑99 (MQ=255)
                                                                                 cgcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa              >  2:2116779/1‑100 (MQ=255)
                                                                                  gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGTAg             >  1:2753415/1‑100 (MQ=255)
                                                                                   cgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAgg            >  2:3224670/1‑100 (MQ=255)
                                                                                    ggTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGc           <  2:860030/100‑1 (MQ=255)
                                                                                    ggTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGc           <  1:90660/100‑1 (MQ=255)
                                                                                     gTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt          >  2:2505301/1‑100 (MQ=255)
                                                                                     gTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt          <  2:2820657/100‑1 (MQ=255)
                                                                                        gTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc       >  2:1839772/1‑100 (MQ=255)
                                                                                          tCTGTCCATAACGCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACa      >  2:2280364/1‑99 (MQ=255)
                                                                                           cTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAtt    <  1:1253152/100‑1 (MQ=255)
                                                                                           cTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAtt    >  1:3145349/1‑100 (MQ=255)
                                                                                           cTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAtt    >  1:3099798/1‑100 (MQ=255)
                                                                                             gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  >  1:1951845/1‑100 (MQ=255)
                                                                                             gTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTGCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTc  <  1:2103883/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTC  >  NC_000913/367733‑367925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: