BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,214	T→C	D237G (GAC→GGC)	ddlA ←	D‑alanine‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,214	0	T	C	100.0%	387.4 / NA	110	D237G (GAC→GGC)	ddlA	D‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (60/50); total (60/50)

Only 100 of 110 total aligned reads displayed.

TACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGATCGACGGCAA  >  NC_000913/400117‑400307

tACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCg                                                                                              <  2:2578923/100‑1 (MQ=255)

 aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGt                                                                                             >  1:3373152/1‑100 (MQ=255)

 aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGt                                                                                             <  2:2198710/100‑1 (MQ=255)

  ccACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGtt                                                                                            <  2:1687051/100‑1 (MQ=255)

  ccACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGtt                                                                                            >  1:2490866/1‑100 (MQ=255)

  ccACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGtt                                                                                            <  2:25450/100‑1 (MQ=255)

   cACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTTGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTg                                                                                           <  2:634569/100‑1 (MQ=255)

   cACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTg                                                                                           >  1:1053667/1‑100 (MQ=255)

   cACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTg                                                                                           >  2:2255668/1‑100 (MQ=255)

   cACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCGCTCCACAGGTGCTGGCTTGCGGATTGCCGTTg                                                                                           <  2:3293880/100‑1 (MQ=255)

    aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                          >  1:2288966/1‑100 (MQ=255)

    aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                          >  1:2999768/1‑100 (MQ=255)

    aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                          >  1:1845762/1‑100 (MQ=255)

    aCTTTCGCGCCAACTTCGTCAATGTACTTGGCGTCGTAGCCATAGAAATCGCTAATGACTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                          <  2:1514696/100‑1 (MQ=255)

      tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGccc                                                                                        <  1:543871/100‑1 (MQ=255)

       ttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCa                                                                                       >  1:3188815/1‑100 (MQ=255)

       ttCGCGCCATCTTCGTCAATCTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACCATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGccc                                                                                        <  1:1171314/99‑1 (MQ=255)

           tagcATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCATTGCCCAGAAc                                                                                   <  1:3246603/97‑1 (MQ=255)

           cgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAc                                                                                   >  2:1525883/1‑100 (MQ=255)

               aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                               <  2:3373204/100‑1 (MQ=255)

               aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                               <  1:3172868/100‑1 (MQ=255)

               aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                               >  2:477485/1‑100 (MQ=255)

                 cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCa                                                                             >  1:905319/1‑100 (MQ=255)

                 cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCa                                                                             >  1:1884335/1‑100 (MQ=255)

                 cTTCGTCAATGTACTTGGTGGCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGGGCa                                                                             >  1:2609135/1‑100 (MQ=255)

                  ttccgcAATGTAGTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGATGCTGACTTTCGGATTCCCGTTGGCCAGAAc                                                                                   >  1:2427563/6‑93 (MQ=255)

                    cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                          >  2:2537811/1‑100 (MQ=255)

                     gTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCg                                                                         >  2:2543775/1‑100 (MQ=255)

                     gTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCg                                                                         <  2:1327758/100‑1 (MQ=255)

                      tCAATGTACTTGGTGTCCTAGGCATAGACATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGa                                                                        <  1:1941402/100‑1 (MQ=255)

                       cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAt                                                                       >  1:3298341/1‑100 (MQ=255)

                       cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAt                                                                       <  1:1178283/100‑1 (MQ=255)

                       cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAt                                                                       >  2:2824795/1‑100 (MQ=255)

                         aTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtcn                                                                     <  1:2480532/100‑2 (MQ=255)

                          tGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtctc                                                                    >  2:408289/1‑100 (MQ=255)

                           gTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCa                                                                   >  1:487677/1‑100 (MQ=255)

                            tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGTTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                  <  1:1899875/100‑1 (MQ=255)

                            tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                  >  1:2564742/1‑100 (MQ=255)

                            tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                  <  1:2160228/100‑1 (MQ=255)

                             aCTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACg                                                                 >  1:1695223/1‑100 (MQ=255)

                             aCTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACg                                                                 <  1:1311687/100‑1 (MQ=255)

                             aCTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACAATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACg                                                                 >  1:2912386/1‑100 (MQ=255)

                              cTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGa                                                                <  2:12295/100‑1 (MQ=255)

                                tGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGAcc                                                              >  1:2445956/1‑100 (MQ=255)

                                 ggTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCt                                                             >  1:3085046/1‑100 (MQ=255)

                                   tgtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCttt                                                           <  1:713589/100‑1 (MQ=255)

                                    gtCGTAGGCATAGAAATCGCTGGTTAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCtt                                                            <  1:2928430/98‑1 (MQ=255)

                                    gtCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTg                                                          >  2:1499503/1‑100 (MQ=255)

                                      cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                        <  2:2445989/100‑1 (MQ=255)

                                      cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCAGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGan                                                        <  1:1930306/100‑2 (MQ=255)

                                       gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                       >  1:1114875/1‑100 (MQ=255)

                                       gTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                       <  1:396366/100‑1 (MQ=255)

                                        tAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATcc                                                      <  1:1366865/100‑1 (MQ=255)

                                           gCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                                   >  1:1116910/1‑100 (MQ=255)

                                            cATAGAAATCGCTGGTGAGTACGATCTCTACACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                  >  2:204818/1‑100 (MQ=255)

                                             aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                 <  2:3011033/100‑1 (MQ=255)

                                             aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                 <  1:3238513/100‑1 (MQ=255)

                                              tAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCt                                                >  1:675744/1‑100 (MQ=255)

                                               aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                               >  1:281210/1‑100 (MQ=255)

                                               aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                               >  1:1612996/1‑100 (MQ=255)

                                               aGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                               <  2:2564776/100‑1 (MQ=255)

                                                gAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTcn                                              <  1:2176759/100‑2 (MQ=255)

                                                 aaaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCaa                                             >  2:1340617/1‑100 (MQ=255)

                                                  aaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                            <  2:1115745/100‑1 (MQ=255)

                                                  aaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                            <  2:3188867/100‑1 (MQ=255)

                                                      gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                        <  2:2002354/100‑1 (MQ=255)

                                                      gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                        <  1:2197432/100‑1 (MQ=255)

                                                       cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                       <  2:2744234/100‑1 (MQ=255)

                                                       cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCATTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                       >  2:2112697/1‑100 (MQ=255)

                                                          gTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtt                                    <  2:3287819/100‑1 (MQ=255)

                                                           tGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACttt                                   <  1:627880/100‑1 (MQ=255)

                                                           tGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACttt                                   >  2:2508212/1‑100 (MQ=255)

                                                           tGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtct                                   >  1:2104660/1‑98 (MQ=255)

                                                            naGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                  >  1:659606/2‑100 (MQ=255)

                                                            gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGTCCATAACTGCGCATACGATCTCACGACCTTTGATCCTTTGCTCAACGATCACtttn                                  <  1:659608/100‑2 (MQ=255)

                                                            gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                  <  1:1242385/100‑1 (MQ=255)

                                                            gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                  >  1:401349/1‑100 (MQ=255)

                                                            gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                  >  1:974017/1‑100 (MQ=255)

                                                              gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGAACACTTTaa                                 >  1:2104861/1‑98 (MQ=255)

                                                                aCGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAt                              >  2:1933876/1‑100 (MQ=255)

                                                                      tctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaact                        <  1:1487665/100‑1 (MQ=255)

                                                                       ctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCCGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCCCTTTATGAtcgaactc                       >  1:526398/1‑100 (MQ=255)

                                                                       ctcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactc                       >  1:146759/1‑100 (MQ=255)

                                                                        tcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                      <  1:3158800/100‑1 (MQ=255)

                                                                        tcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                      <  2:1714198/100‑1 (MQ=255)

                                                                         ccACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCCCGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcga                     >  2:236214/1‑100 (MQ=255)

                                                                          cacaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa                    >  2:1969860/1‑100 (MQ=255)

                                                                          cacaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa                    >  2:110608/1‑100 (MQ=255)

                                                                            caGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACg                  <  2:3372858/100‑1 (MQ=255)

                                                                               ccgatctcTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑a              <  2:1569330/93‑1 (MQ=255)

                                                                                tGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑Ag             >  2:1354234/1‑100 (MQ=255)

                                                                                 gCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGa            >  2:530209/1‑100 (MQ=255)

                                                                                  cTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGAt           >  2:1387413/1‑100 (MQ=255)

                                                                                   tGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGATc          >  1:1569451/1‑100 (MQ=255)

                                                                                    ggCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGATCg         >  2:3411161/1‑100 (MQ=255)

                                                                                      cTTGCGGTTTGCCGTTGTCCGGATCTGCGCATTCGATCTCACGCCCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGAt           <  1:923894/96‑1 (MQ=255)

                                                                                      cTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAAGATCgg        >  1:1569308/1‑99 (MQ=255)

                                                                                       ttGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGATCGACg      <  1:1812305/100‑1 (MQ=255)

                                                                                         gCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGATCGACGGc    >  2:2657215/1‑100 (MQ=255)

                                                                                           ggATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGATCGACGGCaa  >  2:2546764/1‑100 (MQ=255)

TACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCC‑AGATCGACGGCAA  >  NC_000913/400117‑400307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`