Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 459,256 T→C D123D (GAT→GAC lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,2560TC95.4% 347.2 / 1.5 110D123D (GAT→GAClonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (51/53);  total (55/55)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 110 total aligned reads displayed.

GGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACG  >  NC_000913/459160‑459349
                                                                                                |                                                                                             
ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAg                                                                                            <  2:3157119/100‑1 (MQ=255)
ggTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAg                                                                                            <  2:2846854/100‑1 (MQ=255)
 gTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGc                                                                                           <  1:3453578/100‑1 (MQ=255)
  tCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCg                                                                                          <  2:3178592/100‑1 (MQ=255)
    cttccgatctaGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCggg                                                                                        <  1:73914/90‑1 (MQ=255)
     aGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGa                                                                                       <  2:561842/100‑1 (MQ=255)
      ggggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGaa                                                                                      >  1:1718847/1‑100 (MQ=255)
       gggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAAc                                                                                     <  2:2913526/100‑1 (MQ=255)
       gggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAAc                                                                                     >  2:1647711/1‑100 (MQ=255)
        ggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACa                                                                                    >  1:2999878/1‑100 (MQ=255)
        ggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACa                                                                                    >  2:724376/1‑100 (MQ=255)
        ggTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACa                                                                                    >  1:1088068/1‑100 (MQ=255)
         gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAg                                                                                   >  1:1850211/1‑100 (MQ=255)
         gTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAg                                                                                   >  2:365373/1‑100 (MQ=255)
             cAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATGTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAg                                                                               <  2:2454583/100‑1 (MQ=255)
             cAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAg                                                                               <  2:3088849/100‑1 (MQ=255)
              aGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGagatcggaag                                                                              >  2:73915/1‑91 (MQ=255)
                cttccgatcttttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGc                                                                            <  1:2552497/90‑1 (MQ=255)
                    cgcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGt                                                                        <  2:2815582/100‑1 (MQ=255)
                     gcgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTg                                                                       >  2:2677851/1‑100 (MQ=255)
                      cgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGACGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGc                                                                      >  1:2936269/1‑100 (MQ=255)
                      cgTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGAATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGc                                                                      >  1:2936138/1‑100 (MQ=255)
                        tATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGt                                                                    >  1:2522427/1‑100 (MQ=255)
                         aTTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTa                                                                   >  1:716609/1‑100 (MQ=255)
                         aTTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTa                                                                   >  1:137457/1‑100 (MQ=255)
                          tttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGcagatcggaag                                                                  >  2:2552531/1‑90 (MQ=255)
                          tttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTAc                                                                  >  2:2433953/1‑100 (MQ=255)
                           ttCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACt                                                                 >  2:1904843/1‑100 (MQ=255)
                            tCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTg                                                                <  2:3117147/100‑1 (MQ=255)
                             cTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAACTGCTGGTGCGTACTGc                                                               >  1:450695/1‑100 (MQ=255)
                              tGCGCTCTCTGACAATGGCGAACATTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCa                                                              <  1:1555433/100‑1 (MQ=255)
                              tGCGCGCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCa                                                              <  1:407721/100‑1 (MQ=255)
                               gcgcTCTCTGAGAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGAAGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa                                                             >  1:1834097/1‑100 (MQ=255)
                               gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa                                                             >  1:2992794/1‑100 (MQ=255)
                               gcgcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCaa                                                             >  1:1258948/1‑100 (MQ=255)
                                 gcgatctGATAATGACGAAGACTAATCTGCGAAGACGGAGAATAAGGAGAAGCCAACCATTGATGAGCGGGATTAGGAAGTGCTGGTGCg                                                                     <  1:2170557/86‑1 (MQ=255)
                                 gcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATc                                                           <  2:1703022/100‑1 (MQ=255)
                                 gcTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATc                                                           <  1:2446741/100‑1 (MQ=255)
                                  ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                          >  1:1039768/1‑100 (MQ=255)
                                  ctctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCa                                                          >  2:1958210/1‑100 (MQ=255)
                                   tctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAg                                                         <  1:2801108/100‑1 (MQ=255)
                                   tctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAg                                                         <  2:1607121/100‑1 (MQ=255)
                                    ctctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGGCGCCGACCATTGACGAGCGGGAACAGGAAGTGCGGGTGCGTACTGCAATCAGc                                                        >  1:3202030/1‑100 (MQ=255)
                                     tctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGcc                                                       <  2:1338499/100‑1 (MQ=255)
                                      ctGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCa                                                      >  1:1071445/1‑100 (MQ=255)
                                       tGACAGTGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAg                                                     <  1:2141089/100‑1 (MQ=255)
                                       tGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAg                                                     <  1:3472478/100‑1 (MQ=255)
                                       tGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAg                                                     >  1:1686539/1‑100 (MQ=255)
                                       tGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAg                                                     >  2:3005077/1‑100 (MQ=255)
                                        gACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGt                                                    >  1:1219503/1‑100 (MQ=255)
                                         aCAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGtt                                                   <  2:2916377/100‑1 (MQ=255)
                                          cAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTc                                                  <  1:3265960/100‑1 (MQ=255)
                                          cAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTc                                                  >  1:827071/1‑100 (MQ=255)
                                           aaTGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCg                                                 <  2:1395218/100‑1 (MQ=255)
                                             tGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGaa                                               <  2:1076925/100‑1 (MQ=255)
                                              ggCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAg                                              >  2:1078978/1‑100 (MQ=255)
                                                 gAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCt                                           >  1:131362/1‑100 (MQ=255)
                                                   acacTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTAc                                         <  2:2214764/100‑1 (MQ=255)
                                                   acacTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTAc                                         <  2:1701673/100‑1 (MQ=255)
                                                   acacTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTAc                                         <  2:137459/100‑1 (MQ=255)
                                                      cTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATc                                      >  1:1085523/1‑100 (MQ=255)
                                                       ttttCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCa                                     <  1:3050098/100‑1 (MQ=255)
                                                          tCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAg                                   <  2:2631772/99‑1 (MQ=255)
                                                           cTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGcn                                 <  2:2666078/100‑2 (MQ=255)
                                                           cTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCt                                 <  2:1088082/100‑1 (MQ=255)
                                                           cTGCGAAGGCGGAGTATCTGGAGTCGCCGACCAGTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCt                                 >  1:2293101/1‑100 (MQ=255)
                                                            tGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTg                                <  2:1006415/100‑1 (MQ=255)
                                                             gCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGa                               <  1:565685/100‑1 (MQ=255)
                                                             gCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGa                               <  1:85838/100‑1 (MQ=255)
                                                              cGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTgca                              >  1:541795/1‑98 (MQ=255)
                                                              cGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGaa                              <  2:2987315/100‑1 (MQ=255)
                                                              cGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGaa                              <  1:146308/100‑1 (MQ=255)
                                                              cGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGaa                              >  1:2309527/1‑100 (MQ=255)
                                                              cGAAGGCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGaa                              >  1:719341/1‑100 (MQ=255)
                                                                   gCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCGAGCTGAACaaaa                         <  2:2282636/100‑1 (MQ=255)
                                                                   gCGGAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaa                         <  2:3401691/100‑1 (MQ=255)
                                                                    cttccgaTCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaaa                        <  2:2459771/94‑1 (MQ=255)
                                                                    cGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaaa                        >  1:3102891/1‑100 (MQ=255)
                                                                     ggAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaaaa                       >  1:1758531/1‑100 (MQ=255)
                                                                      gAGTATCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaaaaa                      >  1:2582907/1‑100 (MQ=255)
                                                                         tATCAGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAAAAAAAAAAAAAAATcc                   <  1:3057204/100‑1 (MQ=255)
                                                                          aTCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATccc                  >  2:1417385/1‑100 (MQ=255)
                                                                           tCTGGAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCcca                 >  2:1660698/1‑100 (MQ=255)
                                                                              ggAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACaaaaaagatcggaag              >  1:2459738/1‑91 (MQ=255)
                                                                              ggAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacca              <  2:1630553/100‑1 (MQ=255)
                                                                              ggAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacca              >  1:1343863/1‑100 (MQ=255)
                                                                              ggAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCccacc               >  1:1644599/1‑99 (MQ=255)
                                                                               gAGTCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAg             <  1:1713059/100‑1 (MQ=255)
                                                                                  tCGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAg          <  2:2050281/100‑1 (MQ=255)
                                                                                  tCGCCGACCATTGACGAGCGGGAACAGGAACTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAg          <  2:450700/100‑1 (MQ=255)
                                                                                   cGCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGt         >  1:292917/1‑100 (MQ=255)
                                                                                    gCCGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTg        >  2:2966016/1‑100 (MQ=255)
                                                                                      cGACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCt      >  1:2204383/1‑100 (MQ=255)
                                                                                       gACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTg     <  1:1252373/100‑1 (MQ=255)
                                                                                       gACCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGAAATCAGCCAGTTCGAAGGATAAATCAAGATAAAAAAAAAAATCCCACCAGAAGTGCTg     <  1:3212932/100‑1 (MQ=255)
                                                                                        aCCATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGa    <  1:724367/100‑1 (MQ=255)
                                                                                         ccATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc   >  2:2266221/1‑100 (MQ=255)
                                                                                         ccATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGAc   >  1:3140086/1‑100 (MQ=255)
                                                                                         ccATTGACGAGCGGGAACAGGAAGTGCTCGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCGAGCTGAACAAAAAAATCCCACCAGAAGTGCTGa    <  1:2095411/99‑1 (MQ=255)
                                                                                          cATTGACGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACg  >  1:1583364/1‑100 (MQ=255)
                                                                                                |                                                                                             
GGTCGAGGGGTTACAGCGCGCGCGTATTTCTGCGCTCTCTGACAATGGCGAACACTTTTCTGCGAAGGCGGAGTATCTGGAGTCGCCGACCATTGATGAGCGGGAACAGGAAGTGCTGGTGCGTACTGCAATCAGCCAGTTCGAAGGCTACATCAAGCTGAACAAAAAAATCCCACCAGAAGTGCTGACG  >  NC_000913/459160‑459349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: