Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 459,642 A→G E252G (GAA→GGA)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,6420AG99.2% 417.4 / ‑4.8 122E252G (GAA→GGA) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (56/65);  total (56/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

Only 100 of 122 total aligned reads displayed.

CAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGA  >  NC_000913/459545‑459737
                                                                                                 |                                                                                               
cAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAg                                                                                               <  2:2200646/100‑1 (MQ=255)
  gATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGcc                                                                                             <  2:766302/100‑1 (MQ=255)
   aTGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGccc                                                                                            >  2:301586/1‑100 (MQ=255)
     ggAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTg                                                                                          >  1:752105/1‑100 (MQ=255)
      gagaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGa                                                                                         >  1:415712/1‑100 (MQ=255)
      gagaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGa                                                                                         >  1:2648841/1‑100 (MQ=255)
       agaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGaa                                                                                        >  1:2310243/1‑100 (MQ=255)
        gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAg                                                                                       <  2:1965254/100‑1 (MQ=255)
        gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAg                                                                                       <  1:3364236/100‑1 (MQ=255)
        gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAg                                                                                       <  1:952426/100‑1 (MQ=255)
        gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAg                                                                                       <  2:1210703/100‑1 (MQ=255)
        gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAg                                                                                       <  2:1835614/100‑1 (MQ=255)
        gaAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAg                                                                                       >  2:2582813/1‑100 (MQ=255)
        gaAATCCCAGCGGGAGTACTATCGGAACGAGCGAATAAAAGCTATTCAGAAAGAACTCGGTGAAATGGACCACGCGCCGGACGAAAACGGAGCCCTGAAg                                                                                       <  2:1184430/100‑1 (MQ=255)
             cccAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTGTTCAGAAAGAACTCGGTGGAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCa                                                                                   >  1:2438844/1‑99 (MQ=255)
             cccAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCaa                                                                                  <  2:1909613/100‑1 (MQ=255)
             cccAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCaa                                                                                  >  1:160599/1‑100 (MQ=255)
              ccAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGGCGCGCCGGACGAAAACGGAGCCCTGAAGCGCaaa                                                                                 <  2:659150/100‑1 (MQ=255)
              ccAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCaaa                                                                                 <  2:2133862/100‑1 (MQ=255)
              ccAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCaaa                                                                                 >  1:2623011/1‑100 (MQ=255)
               cAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCaaaa                                                                                <  2:644099/100‑1 (MQ=255)
                  cGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCg                                                                             <  1:1356423/100‑1 (MQ=255)
                     gAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGAcg                                                                          <  1:462635/100‑1 (MQ=255)
                           tATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGa                                                                    <  1:2568001/100‑1 (MQ=255)
                            aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGaa                                                                   <  2:983113/100‑1 (MQ=255)
                            aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGaa                                                                   >  1:2785728/1‑100 (MQ=255)
                            aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGaa                                                                   >  1:1921779/1‑100 (MQ=255)
                            aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGaa                                                                   >  1:1033561/1‑100 (MQ=255)
                              cTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAg                                                                  >  1:2819158/1‑99 (MQ=255)
                              cTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGa                                                                 >  2:1841579/1‑100 (MQ=255)
                              cTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGa                                                                 >  2:362568/1‑100 (MQ=255)
                               tGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGAt                                                                >  2:1284734/1‑100 (MQ=255)
                                gAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCTGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATg                                                               >  1:2306507/1‑100 (MQ=255)
                                gAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATg                                                               >  1:1011982/1‑100 (MQ=255)
                                 aaCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGc                                                              <  1:2646679/100‑1 (MQ=255)
                                  aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGcc                                                             >  2:493237/1‑100 (MQ=255)
                                  aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGcc                                                             <  1:1807511/100‑1 (MQ=255)
                                  aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGcc                                                             <  1:567793/100‑1 (MQ=255)
                                   cGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCg                                                            >  2:2944002/1‑100 (MQ=255)
                                   cGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCg                                                            <  2:260390/100‑1 (MQ=255)
                                    gAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGa                                                           <  2:2763614/100‑1 (MQ=255)
                                    gAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGa                                                           <  1:2138273/100‑1 (MQ=255)
                                     aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGaa                                                          <  1:3348456/100‑1 (MQ=255)
                                        aaaTGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAaga                                                       <  1:1339314/100‑1 (MQ=255)
                                          ataaaaGCTATTCAGAAAGAACTCGGTCAAATGGACGACGCGGCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAagag                                                      <  1:181576/96‑1 (MQ=255)
                                          aTGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAgg                                                     <  1:742679/100‑1 (MQ=255)
                                          aTGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGC‑AAATCGACGCGGCGAAGATGCCGAAAGAGGc                                                    <  1:140885/100‑1 (MQ=255)
                                           tGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGc                                                    <  2:723962/100‑1 (MQ=255)
                                            gAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCa                                                   <  1:100268/100‑1 (MQ=255)
                                            gAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCa                                                   <  1:123880/100‑1 (MQ=255)
                                            gAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCa                                                   <  1:3353939/100‑1 (MQ=255)
                                             aaaGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCaa                                                  <  1:2495012/100‑1 (MQ=255)
                                             aaaGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCaa                                                  >  2:2519826/1‑100 (MQ=255)
                                              aaGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAACGAGGCaaa                                                 >  2:411060/1‑100 (MQ=255)
                                              aaGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCaaa                                                 <  1:2504453/100‑1 (MQ=255)
                                                gCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAag                                               >  2:2183435/1‑100 (MQ=255)
                                                 cTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAaga                                              <  2:1388903/100‑1 (MQ=255)
                                                  tATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAagag                                             >  1:1738647/1‑100 (MQ=255)
                                                  tATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAag                                               <  1:2589008/98‑1 (MQ=255)
                                                   aTTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAgaga                                            <  1:64419/100‑1 (MQ=255)
                                                    ttCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGaa                                           >  1:1906828/1‑100 (MQ=255)
                                                    ttCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGaa                                           <  2:973570/100‑1 (MQ=255)
                                                     tCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGaaa                                          >  1:1949454/1‑100 (MQ=255)
                                                     tCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGaaa                                          >  1:2858607/1‑100 (MQ=255)
                                                      cAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGATAg                                         >  1:331200/1‑100 (MQ=255)
                                                      cAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAg                                         <  2:1619709/100‑1 (MQ=255)
                                                      cAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAg                                         <  1:287190/100‑1 (MQ=255)
                                                      cAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAg                                         >  2:2973958/1‑100 (MQ=255)
                                                       agaaagaaCTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGc                                        >  1:1610173/1‑100 (MQ=255)
                                                               cTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCag                                >  2:2564128/1‑100 (MQ=255)
                                                               cTCGGTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCag                                >  2:2947391/1‑100 (MQ=255)
                                                                  ggTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt                             >  1:1769919/1‑100 (MQ=255)
                                                                  ggTGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt                             >  2:1661510/1‑100 (MQ=255)
                                                                    tGAAATGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTg                           <  1:2494863/100‑1 (MQ=255)
                                                                      aaaTGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCa                         <  2:1659870/100‑1 (MQ=255)
                                                                        aTGGACGACGCGCTGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGa                       <  2:2306539/100‑1 (MQ=255)
                                                                         tGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGaa                      >  1:1943229/1‑100 (MQ=255)
                                                                         tGGACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGaa                      >  1:418020/1‑100 (MQ=255)
                                                                          ggACGACGCGCCGGACGAAAACGGAGCCCTGAAGCGCACAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAg                     >  1:2136507/1‑100 (MQ=255)
                                                                            acgacgCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCt                   <  1:1772782/100‑1 (MQ=255)
                                                                             cgacgCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTg                  >  2:552091/1‑100 (MQ=255)
                                                                             cgacgCGCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTg                  >  2:2146248/1‑100 (MQ=255)
                                                                                cgcgCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGaaa               >  1:547857/1‑100 (MQ=255)
                                                                                cgcgCCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGaaa               >  2:2537092/1‑100 (MQ=255)
                                                                                  cgcCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAat             <  2:547863/100‑1 (MQ=255)
                                                                                  cgcCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAat             >  1:1107957/1‑100 (MQ=255)
                                                                                   gcCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatg            >  2:1788209/1‑100 (MQ=255)
                                                                                   gcCGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatg            <  1:1546592/100‑1 (MQ=255)
                                                                                    ccGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga           <  1:3102310/100‑1 (MQ=255)
                                                                                    ccGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga           <  2:1853641/100‑1 (MQ=255)
                                                                                     cGGACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat          <  1:1609674/100‑1 (MQ=255)
                                                                                      ggACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgatg         <  1:1841554/100‑1 (MQ=255)
                                                                                      ggACGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgatg         <  1:1059796/100‑1 (MQ=255)
                                                                                       gACGAAAACGGAGCCCGGAAGCGCAAAAGCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGTAAGCAGAGTGGCAGAAGCTGACAatgatg         <  1:2187499/99‑1 (MQ=255)
                                                                                         cGAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGtct      >  2:2869247/1‑100 (MQ=255)
                                                                                          gAAAACGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGtctc     >  2:1662086/1‑100 (MQ=255)
                                                                                           aaaaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTcc    <  2:1950149/100‑1 (MQ=255)
                                                                                           aaaaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTcc    <  2:3466059/100‑1 (MQ=255)
                                                                                           aaaaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTcc    >  1:748438/1‑100 (MQ=255)
                                                                                             aaCGGAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGa  >  2:378770/1‑100 (MQ=255)
                                                                                                 |                                                                                               
CAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGA  >  NC_000913/459545‑459737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑