BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	88,208	A→T	T61S (ACA→TCA)	cra →	transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	88,208	0	A	T	100.0%	363.2 / NA	111	T61S (ACA→TCA)	cra	transcriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (54/57); total (54/57)

Only 100 of 111 total aligned reads displayed.

GTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATC  >  NC_000913/88113‑88304

gtgtGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACg                                                                                              <  1:794766/100‑1 (MQ=255)

  gtgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGtt                                                                                            <  1:3040782/100‑1 (MQ=255)

  gtgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGtt                                                                                            >  2:1761204/1‑100 (MQ=255)

   tgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTc                                                                                           >  2:1364556/1‑100 (MQ=255)

   tgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTc                                                                                           >  2:1615268/1‑100 (MQ=255)

   tgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTc                                                                                           >  1:2543665/1‑100 (MQ=255)

    gAGCGATAAAGCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTCGACGCTCACGTTc                                                                                           <  1:2145336/99‑1 (MQ=255)

    gAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCt                                                                                          >  2:1217038/1‑100 (MQ=255)

    gAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCt                                                                                          >  2:1777153/1‑100 (MQ=255)

    gAGCGACAAAAACGTTGAAAAAGTCATGGCAGTAGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCt                                                                                          >  2:1706423/1‑100 (MQ=255)

      gCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGTCTTCGTGCTGGACGCTCACGTTCTAt                                                                                        <  1:3139903/100‑1 (MQ=255)

      gCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTAt                                                                                        <  1:9656/100‑1 (MQ=255)

      gCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTAt                                                                                        >  2:2513750/1‑100 (MQ=255)

      gCGACAAAACCGTTAAAAAAGACATGGCTGTGGTGCGTGAGCACAATTACCACCTGAACGCGGTGGCAGCTGGGCTTCGTGCTTGACGCTCACGTTCTAt                                                                                        >  1:2378466/1‑100 (MQ=255)

       cGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTAtt                                                                                       <  1:1769168/100‑1 (MQ=255)

        gACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTg                                                                                      >  2:12776/1‑100 (MQ=255)

        gACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTg                                                                                      >  1:3395575/1‑100 (MQ=255)

        gACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTg                                                                                      >  2:546108/1‑100 (MQ=255)

         aCAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTgg                                                                                     >  1:1481486/1‑100 (MQ=255)

           aaaaCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTggcc                                                                                   <  1:2758903/100‑3 (MQ=255)

              aCCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtg                                                                                <  1:149894/100‑1 (MQ=255)

               ccGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgt                                                                               <  1:1118256/100‑1 (MQ=255)

               ccGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgt                                                                               <  1:2924136/100‑1 (MQ=255)

                cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACCATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTtgtg                                                                              <  1:171297/100‑1 (MQ=255)

                 gTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTCCCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGa                                                                             <  1:1596490/100‑1 (MQ=255)

                 gTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGa                                                                             >  1:454052/1‑100 (MQ=255)

                   tGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATc                                                                           <  2:1866289/100‑1 (MQ=255)

                    gAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATcc                                                                          >  2:1962056/1‑100 (MQ=255)

                    gAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATcc                                                                          <  2:632627/100‑1 (MQ=255)

                     aaaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATccc                                                                         >  1:1477891/1‑100 (MQ=255)

                      aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATcccc                                                                        >  2:3055432/1‑100 (MQ=255)

                      aaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATccc                                                                         >  1:3187389/1‑99 (MQ=255)

                       aaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCg                                                                       <  1:2384128/100‑1 (MQ=255)

                       aaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCg                                                                       <  2:1351128/100‑1 (MQ=255)

                          gTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATc                                                                    >  1:2411261/1‑100 (MQ=255)

                          gTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATc                                                                    >  1:2633355/1‑100 (MQ=255)

                          gTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATc                                                                    <  2:175159/100‑1 (MQ=255)

                           tCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCt                                                                   <  1:841191/100‑1 (MQ=255)

                           tCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCt                                                                   <  1:2848731/100‑1 (MQ=255)

                            cATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTg                                                                  >  1:3321798/1‑100 (MQ=255)

                             aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTgg                                                                 <  1:745084/100‑1 (MQ=255)

                             aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTgg                                                                 <  2:3010086/100‑1 (MQ=255)

                             aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTgg                                                                 <  2:2468666/100‑1 (MQ=255)

                              tGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGga                                                                <  2:2686507/100‑1 (MQ=255)

                              tGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGga                                                                <  2:837924/100‑1 (MQ=255)

                               ggCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgag                                                               >  1:1996149/1‑100 (MQ=255)

                               ggCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGCGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgag                                                               <  1:1895886/100‑1 (MQ=255)

                               ggCTGTGGTGCGAGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgag                                                               >  1:1271191/1‑100 (MQ=255)

                                gCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGgaga                                                              <  2:3103065/100‑1 (MQ=255)

                                  tgtgGTGCGTGAGCACAATTACCACCCGAACGCGGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAac                                                            >  1:2289015/1‑100 (MQ=255)

                                  tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAac                                                            <  2:756191/100‑1 (MQ=255)

                                  tgtgGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAac                                                            <  2:416743/100‑1 (MQ=255)

                                     ggtgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACAcc                                                         <  1:934616/100‑1 (MQ=255)

                                       tgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAg                                                       <  2:3072925/100‑1 (MQ=255)

                                       tgCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACCTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAg                                                       >  1:658547/1‑100 (MQ=255)

                                        gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGc                                                      <  2:3343071/100‑1 (MQ=255)

                                        gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGc                                                      <  1:2682811/100‑1 (MQ=255)

                                        gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTAACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGc                                                      >  1:2219461/1‑100 (MQ=255)

                                         cGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCt                                                     >  1:254433/1‑100 (MQ=255)

                                         cGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCt                                                     >  1:2759049/1‑100 (MQ=255)

                                           tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCtat                                                   >  2:1495668/1‑100 (MQ=255)

                                           tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGCGATCCCCGATCTGGAGAACACCAGCtat                                                   >  2:919347/1‑100 (MQ=255)

                                            gAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCtata                                                  >  1:975643/1‑100 (MQ=255)

                                             aGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAc                                                 >  1:1973352/1‑100 (MQ=255)

                                             aGCACAATTACCACCCGAACGCCGTCGCAGCTGGGCTTCGTGCTGGACGCTCACTTTCTATTGGGCTTGTGATCCCCGATCTGGAGAACACCAGCgata                                                  <  2:945021/99‑1 (MQ=255)

                                              gCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAcc                                                >  2:942501/1‑100 (MQ=255)

                                              gCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAcc                                                >  2:589046/1‑100 (MQ=255)

                                              gCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATAcc                                                >  1:404114/1‑100 (MQ=255)

                                                 caATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGc                                             >  1:14048/1‑100 (MQ=255)

                                                 caATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGc                                             >  2:2770082/1‑100 (MQ=255)

                                                  aaTTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCa                                            <  1:391813/100‑1 (MQ=255)

                                                      accaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCTATCTGGTGAACACCAGCTATACCCGCATCg                                         <  1:1665706/99‑1 (MQ=255)

                                                       ccaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCt                                       <  2:2960370/100‑1 (MQ=255)

                                                       ccaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCt                                       >  1:2960322/1‑100 (MQ=255)

                                                        caccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                      <  2:3088343/100‑1 (MQ=255)

                                                         accCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTaa                                     <  1:1450463/100‑1 (MQ=255)

                                                         accCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTaa                                     >  2:3031624/1‑100 (MQ=255)

                                                         accCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTa                                      <  2:113698/99‑1 (MQ=255)

                                                          cccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAAc                                    <  2:2888732/100‑1 (MQ=255)

                                                          cccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAAc                                    <  2:454057/100‑1 (MQ=255)

                                                            cGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGCTCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAAcca                                  >  2:653375/1‑98 (MQ=255)

                                                               aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATcn                               <  1:2579913/100‑2 (MQ=255)

                                                               aCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCt                               <  2:856718/100‑1 (MQ=255)

                                                                cGCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCtt                              >  1:1592754/1‑100 (MQ=255)

                                                                 gCCGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTg                             <  1:1435705/100‑1 (MQ=255)

                                                                  ccGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGa                            >  2:987421/1‑100 (MQ=255)

                                                                  ccGTGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCAATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGa                            <  1:2338642/100‑1 (MQ=255)

                                                                     tGGCAGCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACg                         <  2:2063000/100‑1 (MQ=255)

                                                                          gCTGGGCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAgg                    <  1:149167/100‑1 (MQ=255)

                                                                             gggCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGgcgc                 >  2:3308586/1‑100 (MQ=255)

                                                                             gggCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGgcgc                 <  1:3249607/100‑1 (MQ=255)

                                                                              ggCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGcgcg                <  2:3008932/100‑1 (MQ=255)

                                                                               gCTTCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCgg               >  1:1367720/1‑100 (MQ=255)

                                                                                 ttCGTGCTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCa             >  2:2197740/1‑100 (MQ=255)

                                                                                     tGCTAGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgg         <  2:695500/100‑1 (MQ=255)

                                                                                       cTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgggg       >  2:554514/1‑100 (MQ=255)

                                                                                       cTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgggg       <  2:796434/100‑1 (MQ=255)

                                                                                       cTGGACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACgggg       <  1:3392614/100‑1 (MQ=255)

                                                                                          gACGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTa    <  1:1100718/100‑1 (MQ=255)

                                                                                            cGCTCACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATc  >  2:81759/1‑100 (MQ=255)

GTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATC  >  NC_000913/88113‑88304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`