Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 532,380 A→G intergenic (‑159/‑71) allS ← / → allA allD operon transcriptional activator/ureidoglycolate lyase, releasing urea

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913532,3800AG98.7% 495.7 / ‑5.1 149intergenic (‑159/‑71)allS/allAallD operon transcriptional activator/ureidoglycolate lyase, releasing urea
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (71/76);  total (71/78)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

TATTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTAAG  >  NC_000913/532283‑532476
                                                                                                 |                                                                                                
tATTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGtt                                                                                                >  2:1514866/1‑100 (MQ=255)
 aTTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTg                                                                                               >  1:1479830/1‑100 (MQ=255)
   ttttACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGcc                                                                                             <  1:2777162/100‑1 (MQ=255)
    tgtACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCt                                                                                            <  1:3078700/98‑1 (MQ=255)
     ttACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTg                                                                                           <  1:1437344/100‑1 (MQ=255)
      tACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGt                                                                                          >  1:994829/1‑100 (MQ=255)
       aCATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGtt                                                                                         <  2:2626730/100‑1 (MQ=255)
       aCATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGtt                                                                                         <  2:2038787/100‑1 (MQ=255)
         aTGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAt                                                                                       >  2:308070/1‑100 (MQ=255)
         aTGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAt                                                                                       >  1:2312613/1‑100 (MQ=255)
         aTGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAt                                                                                       <  1:544265/100‑1 (MQ=255)
          tGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAtt                                                                                      >  2:2158262/1‑100 (MQ=255)
          tGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAtt                                                                                      >  2:1953375/1‑100 (MQ=255)
          tGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTAtt                                                                                      <  1:2520001/100‑1 (MQ=255)
           gATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTg                                                                                     >  1:1110758/1‑100 (MQ=255)
             tAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGaa                                                                                   <  1:2379944/100‑1 (MQ=255)
             tAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGaa                                                                                   <  1:2631290/100‑1 (MQ=255)
             tAACCCTATTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGa                                                                                    <  2:2752769/99‑1 (MQ=255)
              aaCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGaaa                                                                                  <  2:1529505/100‑1 (MQ=255)
               aCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAc                                                                                 >  1:1474950/1‑100 (MQ=255)
               aCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAc                                                                                 <  1:3097231/100‑1 (MQ=255)
               aCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAc                                                                                 >  2:2776034/1‑100 (MQ=255)
               aCCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAc                                                                                 <  1:1751408/100‑1 (MQ=255)
                cccTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAcc                                                                                >  2:779627/1‑100 (MQ=255)
                cccTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAcc                                                                                >  1:2490144/1‑100 (MQ=255)
                 ccTGTTCAATTTGTGGACTAAATCTAGTTGTTTAAAAATATTCCATCGTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAAcc                                                                                <  1:1198308/99‑1 (MQ=255)
                    gTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGc                                                                            >  2:3275154/1‑100 (MQ=255)
                    gTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGc                                                                            >  2:403535/1‑100 (MQ=255)
                    gTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGc                                                                            <  1:1811676/100‑1 (MQ=255)
                       cAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGa                                                                         >  1:201395/1‑100 (MQ=255)
                        aaTTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGAc                                                                        <  2:1894133/100‑1 (MQ=255)
                         aTTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGAcc                                                                       <  2:3009363/100‑1 (MQ=255)
                             gtgGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTc                                                                   <  1:106199/100‑1 (MQ=255)
                               ggACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTcgg                                                                 >  1:3149631/1‑100 (MQ=255)
                                    aaaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtg                                                            <  2:2401579/100‑1 (MQ=255)
                                    aaaTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtg                                                            >  2:1224008/1‑100 (MQ=255)
                                      aTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtggt                                                          <  2:1713840/100‑1 (MQ=255)
                                      aTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtggt                                                          <  2:846057/100‑1 (MQ=255)
                                      aTCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCggtggt                                                          <  2:2909027/100‑1 (MQ=255)
                                          acttttGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTAAAACTAAGCTGACCTGTCGGCGGTGGTTGa                                                       >  1:586960/3‑99 (MQ=255)
                                           gTTTTGGAAAAATATTCCAACTTTTGTATTGCTGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGTTCGGCGGTGGTTGTAc                                                     >  1:2934823/1‑100 (MQ=255)
                                           gTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAAc                                                     <  2:1289009/100‑1 (MQ=255)
                                            ttttGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTg                                                        <  1:1384316/96‑1 (MQ=255)
                                            ttttGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACg                                                    <  2:722275/100‑1 (MQ=255)
                                             tttGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACgg                                                   >  2:154614/1‑100 (MQ=255)
                                             tttGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACgg                                                   >  1:821606/1‑100 (MQ=255)
                                                 gAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAAtt                                               >  2:3435836/1‑100 (MQ=255)
                                                 gAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAAt                                                >  1:892125/1‑99 (MQ=255)
                                                  aaaaaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTa                                              <  2:1940113/100‑1 (MQ=255)
                                                    aaaTATTTCTATTTTTGAATGGATGTGGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATg                                            <  1:3093499/100‑1 (MQ=255)
                                                    aaaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATg                                            >  1:897839/1‑100 (MQ=255)
                                                    aaaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATg                                            <  2:2312645/100‑1 (MQ=255)
                                                     aaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGt                                           <  1:3297249/100‑1 (MQ=255)
                                                     aaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGt                                           >  2:1400822/1‑100 (MQ=255)
                                                     aaTATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGt                                           <  2:3431084/100‑1 (MQ=255)
                                                      atatTCTAACTTTTGTATTTATGTTCTTCTCTTAAGGTTTTAGGTTACCTGTTATTCAAACCAATCTGAACGTTCGGCGGTGGTTTAACAAAATTAttt                                           <  1:3419679/99‑3 (MQ=255)
                                                      atatTCCAACTTTTGTATTGCTGTTGTACTCTTAAGGTTTTAGGTTGGCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGtt                                          <  1:1508982/100‑1 (MQ=255)
                                                       tatTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTa                                         <  2:1885638/100‑1 (MQ=255)
                                                           ccAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAg                                     >  1:1212017/1‑100 (MQ=255)
                                                            cAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAgg                                    >  1:968989/1‑100 (MQ=255)
                                                            cAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAgg                                    >  1:2074200/1‑100 (MQ=255)
                                                              aCTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGAc                                  <  2:857434/100‑1 (MQ=255)
                                                              aCTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGAc                                  >  1:174723/1‑100 (MQ=255)
                                                                  ttGTATTGATGTTCTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACaaaa                              >  1:2153551/1‑100 (MQ=255)
                                                                   tGTATTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATAAAAAAAAAGAAAAAATGTCGGCGGTGGTTGTAAGGAATGATGTTACATGGAaaaaaa                             <  1:1772326/100‑1 (MQ=255)
                                                                     tATTGATGTTGTTCTCTTAAGGGTTTAGGTTGCGTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACaaaaa                             <  2:1977768/98‑1 (MQ=255)
                                                                      aTTGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGAt                          <  2:2390720/100‑1 (MQ=255)
                                                                       ttGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATg                         <  2:2620492/100‑1 (MQ=255)
                                                                       ttGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATg                         >  2:761038/1‑100 (MQ=255)
                                                                       ttGATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATg                         >  1:1561688/1‑100 (MQ=255)
                                                                         gATGTTGTTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGaa                       >  2:2085176/1‑100 (MQ=255)
                                                                           tgttgtTCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAAc                     <  2:2201478/100‑1 (MQ=255)
                                                                            attgttCACTTAAGGTTTGAGGATGCCTGTTATTGAAACCAAACTAACCGGTAGGTGGTGGTTGAACTGAATTATGAAAAAAAAAAAAAAAGATGAAACt                    <  1:3070034/99‑1 (MQ=255)
                                                                             ttgttCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACtt                   >  2:2740196/1‑100 (MQ=255)
                                                                              tgttCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTc                  >  2:1604986/1‑100 (MQ=255)
                                                                               gttCTCTTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCa                 <  2:3149681/100‑1 (MQ=255)
                                                                                  ctctTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGt              >  2:1082486/1‑100 (MQ=255)
                                                                                   tctTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTa             >  2:1410047/1‑100 (MQ=255)
                                                                                    ctTAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTAt            <  2:2153582/100‑1 (MQ=255)
                                                                                     ttAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTTGGCGGTGGTTGAACGAAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTAtt           >  1:812108/1‑100 (MQ=255)
                                                                                     ttAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTAtt           >  1:978209/1‑100 (MQ=255)
                                                                                     ttAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTAtt           >  1:2548252/1‑100 (MQ=255)
                                                                                     ttAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTAtt           >  1:1972936/1‑100 (MQ=255)
                                                                                     ttAAGGGTTTAGGCTGCCTGCTATTGAAACCAAGCTGACAGGTCGGCGGTGGTTGAACGGAATCATCTTACAAGGCCAAAACGATGAAACTTCAGatat            >  1:1432627/1‑99 (MQ=255)
                                                                                      tAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTa          >  1:3462621/1‑100 (MQ=255)
                                                                                      tAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTa          >  2:561665/1‑100 (MQ=255)
                                                                                      tAAGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTa          >  1:2930314/1‑100 (MQ=255)
                                                                                      tAAGGTTTTAGGTTGCCTGTTAGTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTa          >  1:2025754/1‑100 (MQ=255)
                                                                                       aaGGTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTAc         <  1:1214952/100‑1 (MQ=255)
                                                                                         ggTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCg       >  1:1349867/1‑100 (MQ=255)
                                                                                          gTTTTAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGt      >  1:2115565/1‑100 (MQ=255)
                                                                                           ttttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGtt     <  2:3462675/100‑1 (MQ=255)
                                                                                            tttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa    <  1:1912186/100‑1 (MQ=255)
                                                                                            tttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa    >  1:2581153/1‑100 (MQ=255)
                                                                                            tttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa    <  2:2223804/100‑1 (MQ=255)
                                                                                            tttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa    <  2:1099276/100‑1 (MQ=255)
                                                                                            tttAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa    >  2:1699163/1‑100 (MQ=255)
                                                                                            tttAGGTTGCCTGCTATTGAAACCAAGCTGACCGGTCGGCGGAGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa    >  2:2350745/1‑100 (MQ=255)
                                                                                            tttAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTa    <  1:2084587/100‑1 (MQ=255)
                                                                                              tAGGTTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTAAg  <  2:2025636/100‑1 (MQ=255)
                                                                                                 |                                                                                                
TATTTTTACATGATAACCCTGTTCAATTTGTGGACTAAATCTAGTTTTGGAAAAATATTCCAACTTTTGTATTGATGTTGTTCTCTTAAGGTTTTAGATTGCCTGTTATTGAAACCAAGCTGACCGGTCGGCGGTGGTTGAACGGAATTATGTTACAAGGACAAAAAGATGAAACTTCAGGTATTACCGTTAAG  >  NC_000913/532283‑532476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: