Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 627,309 G→A G414S (GGC→AGC)  entE → 2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913627,3090GA93.8% 379.6 / 9.2 131G414S (GGC→AGC) entE2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (5/3);  new base A (62/60);  total (67/64)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

ACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAA  >  NC_000913/627212‑627404
                                                                                                 |                                                                                               
aCGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGc                                                                                               <  2:728736/100‑1 (MQ=255)
 cGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCg                                                                                              >  1:2836392/1‑100 (MQ=255)
    cTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCgatc                                                                                           <  2:891716/100‑1 (MQ=255)
     tgatgaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCgatct                                                                                          >  2:480035/1‑100 (MQ=255)
      gatgaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCgatctg                                                                                         >  2:2666185/1‑100 (MQ=255)
        tgaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAGAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCgatctgat                                                                                       >  1:1850612/1‑100 (MQ=255)
         gaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCgatctgatc                                                                                      <  1:1085901/100‑1 (MQ=255)
         gaCGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCgatctgatc                                                                                      >  2:1367288/1‑100 (MQ=255)
         gaCGCGCGGGCCCTACACCTTCCGCGGCTATTACATAAGTCGACAGCACAATGCCAGCGCCTTTGATGCGAACGGTTTTTACTGTTCCTGCgatctgat                                                                                       <  1:2394058/99‑1 (MQ=255)
           cgcgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGAtctc                                                                                    >  1:537463/1‑100 (MQ=255)
           cgcgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGAtctc                                                                                    <  2:1380687/100‑1 (MQ=255)
           cgcgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGATTTTACTGTTCCGGCgatctgatct                                                                                     >  2:656540/1‑99 (MQ=255)
             cgcgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTa                                                                                  >  2:1181945/1‑100 (MQ=255)
               cgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTAtt                                                                                <  1:1714687/100‑1 (MQ=255)
               cgGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTAtt                                                                                <  2:1295583/100‑1 (MQ=255)
                gggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTg                                                                               >  1:1360859/1‑100 (MQ=255)
                gggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTg                                                                               <  1:2671536/100‑1 (MQ=255)
                gggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTg                                                                               <  1:754787/100‑1 (MQ=255)
                 ggCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGa                                                                              >  2:1659317/1‑100 (MQ=255)
                  gCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGAt                                                                             >  1:1086472/1‑100 (MQ=255)
                  gCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGAt                                                                             >  2:631070/1‑100 (MQ=255)
                   ccGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATc                                                                            >  2:3063691/1‑100 (MQ=255)
                   ccGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATc                                                                            <  2:2406584/100‑1 (MQ=255)
                    cGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATcc                                                                           <  2:1579709/100‑1 (MQ=255)
                      tACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCag                                                                         >  1:1059137/1‑100 (MQ=255)
                        cacCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCagag                                                                       >  1:2625269/1‑100 (MQ=255)
                        cacCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCagag                                                                       >  2:918604/1‑100 (MQ=255)
                          ccTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAggg                                                                     >  2:1589802/1‑100 (MQ=255)
                          ccTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAggg                                                                     >  1:1295478/1‑100 (MQ=255)
                          ccTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAggg                                                                     >  2:2572462/1‑100 (MQ=255)
                            ttCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAATGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGtt                                                                   <  1:1931548/100‑1 (MQ=255)
                            ttCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGtt                                                                   <  1:1788634/100‑1 (MQ=255)
                            ttCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGtt                                                                   <  1:1350797/100‑1 (MQ=255)
                               cgcgGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACa                                                                <  2:3399862/100‑1 (MQ=255)
                               cgcgGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACa                                                                >  2:93067/1‑100 (MQ=255)
                               cgcgGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACa                                                                <  1:2395269/100‑1 (MQ=255)
                                gcgGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACAt                                                               >  2:108348/1‑100 (MQ=255)
                                  ggCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTAc                                                                 <  1:1124366/96‑1 (MQ=255)
                                   gCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCAc                                                            >  2:1499293/1‑100 (MQ=255)
                                   gCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCAc                                                            >  1:3127701/1‑100 (MQ=255)
                                     tATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCg                                                          >  2:218231/1‑100 (MQ=255)
                                     tATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCg                                                          <  1:1380779/100‑1 (MQ=255)
                                     tATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTAATGTTCCAGCGATCTGATCTCTATTGATCCAGATGGTTACATCAcc                                                           >  1:2979087/1‑99 (MQ=255)
                                      aTTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCg                                                          <  1:1338241/99‑1 (MQ=255)
                                      aTTACAAAAGTCCACAGCACAAAGCCAGCGCTTTTGACGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGt                                                         <  1:2338528/100‑1 (MQ=255)
                                        tACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTTATCCAGAGGGTTACATCACCGTGc                                                       >  2:822226/1‑100 (MQ=255)
                                        tACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGc                                                       <  1:121376/100‑1 (MQ=255)
                                         aCAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCa                                                      <  2:3027600/100‑1 (MQ=255)
                                          cAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGCGGGTTACATCACCGTGCAg                                                     >  2:75356/1‑100 (MQ=255)
                                             aaGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAgggg                                                  <  1:2849245/100‑1 (MQ=255)
                                              aGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGgc                                                 <  2:3343389/100‑1 (MQ=255)
                                              aGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGgc                                                 >  1:1908544/1‑100 (MQ=255)
                                               gTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGgcg                                                >  2:2468394/1‑100 (MQ=255)
                                                 ccACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGcgcg                                              >  1:3096122/1‑100 (MQ=255)
                                                 ccACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGcgcg                                              <  1:631063/100‑1 (MQ=255)
                                                  cacaGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGcgcg                                              >  1:2749104/1‑99 (MQ=255)
                                                  cacaGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCga                                             >  2:2098953/1‑100 (MQ=255)
                                                      gCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGaaa                                         >  2:1526811/1‑100 (MQ=255)
                                                       cacaATGCCAGCGCCTTTGATGCCAACGGTTTTTATTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAg                                        >  2:839778/1‑100 (MQ=255)
                                                       cacaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAg                                        <  2:1086486/100‑1 (MQ=255)
                                                       cacaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAg                                        <  2:2851705/100‑1 (MQ=255)
                                                       cacaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAg                                        <  1:1248082/100‑1 (MQ=255)
                                                        acaATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGa                                       >  2:2428797/1‑100 (MQ=255)
                                                         caATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGAt                                      >  1:504340/1‑100 (MQ=255)
                                                         caATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGAt                                      <  2:2888712/100‑1 (MQ=255)
                                                           aTGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCa                                    <  1:1427642/100‑1 (MQ=255)
                                                            tGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATcg                                    <  1:1719293/99‑2 (MQ=255)
                                                            tGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAg                                   >  2:2957958/1‑100 (MQ=255)
                                                              ccAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGAt                                 <  2:577813/100‑1 (MQ=255)
                                                               cAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCAGCCATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGAtt                                <  1:2635930/100‑1 (MQ=255)
                                                                  cgcCTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAAc                             >  1:854327/1‑100 (MQ=255)
                                                                    ccTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCg                           <  1:2696946/100‑1 (MQ=255)
                                                                    ccTTTGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAAcc                            <  1:2428764/99‑1 (MQ=255)
                                                                        tGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTggc                       >  2:2036692/1‑100 (MQ=255)
                                                                        tGATGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTggc                       <  1:611129/100‑1 (MQ=255)
                                                                           tGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTggcggc                    <  1:3063641/100‑1 (MQ=255)
                                                                           tGCCAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTggcagc                    >  1:2927247/1‑97 (MQ=255)
                                                                            gCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCCGGGGCGCGAGAAAGGTCAGATTAACCGTggcgg                     >  1:621673/1‑98 (MQ=255)
                                                                             ccAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCga                  >  2:2990608/1‑100 (MQ=255)
                                                                             ccAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCga                  >  2:1257923/1‑100 (MQ=255)
                                                                              cAACGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCgag                 >  1:1451703/1‑100 (MQ=255)
                                                                               aaCGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCgaga                <  1:218228/100‑1 (MQ=255)
                                                                               aaCGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCgaga                >  1:1004084/1‑100 (MQ=255)
                                                                                 ctgTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGCTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGagaag              <  1:2792880/98‑1 (MQ=255)
                                                                                 cGGTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCgaga                <  1:1249332/98‑1 (MQ=255)
                                                                                  ggTTTTTACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGagaaga             <  1:2859988/100‑1 (MQ=255)
                                                                                    tttttACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTCCATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATc           >  1:1019756/1‑100 (MQ=255)
                                                                                    tttttACTGTTCCAGCGATCTGATCTCTATTGAACCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTATCCGTGGCGGCGAGAAGATc           <  1:3002441/100‑1 (MQ=255)
                                                                                     ttttACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCg          <  1:2204109/100‑1 (MQ=255)
                                                                                     ttttACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCg          >  2:2244240/1‑100 (MQ=255)
                                                                                     ttttACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCAGCgatcttctcg          <  1:2038332/100‑9 (MQ=255)
                                                                                      tttACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGCGAAGATCGc         >  1:454135/1‑100 (MQ=255)
                                                                                      tttACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCACATTAACCGTGGCGGCGAGAAGATCGc         <  2:2098904/100‑1 (MQ=255)
                                                                                       ttACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCt        >  1:1048727/1‑100 (MQ=255)
                                                                                        tACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTg       <  2:2532273/100‑1 (MQ=255)
                                                                                        tACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTg       <  2:748354/100‑1 (MQ=255)
                                                                                        tACTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTg       <  2:171098/100‑1 (MQ=255)
                                                                                         aCTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGc      <  1:681373/100‑1 (MQ=255)
                                                                                          cTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGagaagacatcatga     >  2:296292/1‑92 (MQ=255)
                                                                                          cTGTTCCAGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAAACGTGGAGGCGAGAAGATCGCTg       >  1:3391076/1‑98 (MQ=255)
                                                                                                 |                                                                                               
ACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGCTATTACAAAAGTCCACAGCACAATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGTGCAGGGGCGCGAGAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAA  >  NC_000913/627212‑627404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: