Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 661,369 T→C N4D (AAT→GAT)  ybeF ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913661,3690TC96.1% 401.8 / 3.0 127N4D (AAT→GAT) ybeFLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (65/57);  total (68/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 129 total aligned reads displayed.

ATAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGC  >  NC_000913/661272‑661463
                                                                                                 |                                                                                              
aTAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCAc                                                                                              <  2:3404062/100‑1 (MQ=255)
aTAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCAc                                                                                              <  1:344267/100‑1 (MQ=255)
 tAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACt                                                                                             >  1:1451667/1‑100 (MQ=255)
   gTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTAt                                                                                           >  1:1636966/1‑100 (MQ=255)
     cAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATcc                                                                                         >  2:282265/1‑100 (MQ=255)
       gAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCAc                                                                                       >  2:2530192/1‑100 (MQ=255)
        aaGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACg                                                                                      <  1:478230/100‑1 (MQ=255)
         cgatctaGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGc                                                                                     <  1:3136076/94‑1 (MQ=255)
         aGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGc                                                                                     <  1:3273874/100‑1 (MQ=255)
          ggTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCa                                                                                    >  2:3039413/1‑100 (MQ=255)
            ttAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACt                                                                                  <  2:269403/100‑1 (MQ=255)
             tAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACtt                                                                                 >  2:765970/1‑100 (MQ=255)
               aGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCagatcg                                                                               >  2:3136126/1‑95 (MQ=255)
                  tCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTcc                                                                            <  1:1413931/100‑1 (MQ=255)
                  tCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTcc                                                                            >  1:3111535/1‑100 (MQ=255)
                  tCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTcc                                                                            <  2:1024881/100‑1 (MQ=255)
                  tCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTcc                                                                            >  2:2368195/1‑100 (MQ=255)
                   cAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCa                                                                           >  2:2705030/1‑100 (MQ=255)
                   cAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCa                                                                           >  1:2939397/1‑100 (MQ=255)
                    aaTATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCaa                                                                          >  2:30191/1‑100 (MQ=255)
                       atTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAAttt                                                                       <  1:1783498/100‑1 (MQ=255)
                          tCGTAAGGTTGTAGATATTTGGGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAt                                                                    >  1:2384758/1‑100 (MQ=255)
                          tCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAt                                                                    <  2:2519146/100‑1 (MQ=255)
                           cGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAtt                                                                   <  1:459592/100‑1 (MQ=255)
                           cGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAtt                                                                   <  1:2324407/100‑1 (MQ=255)
                           cGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTAtt                                                                   >  2:2647691/1‑100 (MQ=255)
                            gTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTc                                                                  <  2:2214125/100‑1 (MQ=255)
                            gTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTc                                                                  <  1:2934565/100‑1 (MQ=255)
                            gTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTc                                                                  <  1:1685304/100‑1 (MQ=255)
                             tAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCa                                                                 >  1:1496710/1‑100 (MQ=255)
                                    gTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGaaa                                                           >  1:1394030/1‑99 (MQ=255)
                                    gTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGaa                                                            >  1:1394091/1‑98 (MQ=255)
                                     tAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGaaaaa                                                         >  2:391429/1‑100 (MQ=255)
                                     tAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGaaaaa                                                         >  2:1962998/1‑100 (MQ=255)
                                      aaaTATTTGTGGTTTAGCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTAACTCCAATTTTATTCATGGaa                                                            >  1:970963/1‑96 (MQ=255)
                                      aaaTATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaat                                                        <  2:2748982/100‑1 (MQ=255)
                                      aaaTATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaat                                                        >  2:3383288/1‑100 (MQ=255)
                                      aaaTATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaat                                                        >  2:2817976/1‑100 (MQ=255)
                                      aaaTATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaat                                                        >  1:691913/1‑100 (MQ=255)
                                       aaTATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaata                                                       <  1:1187557/100‑1 (MQ=255)
                                        atatTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAaataa                                                      >  2:2052179/1‑100 (MQ=255)
                                          atTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAataata                                                    <  2:1706547/100‑1 (MQ=255)
                                          atTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAataata                                                    <  1:742561/100‑1 (MQ=255)
                                          atTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAataata                                                    >  1:383281/1‑100 (MQ=255)
                                            ttGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATAtt                                                  <  1:3257194/100‑1 (MQ=255)
                                               tgGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTaa                                               >  1:3293622/1‑100 (MQ=255)
                                                ggTTTACCTTATGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCAACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTaaa                                              >  1:3370085/1‑100 (MQ=255)
                                                  tttACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTaaaaa                                            <  1:953825/100‑1 (MQ=255)
                                                     aaataaagacaacTTTCTGCTTAAACAGGGTTAAATTTGATTATCACTATCCACGCAATTACTACAATTTTATTCTTGGAAAAAAAAAAAAAAAAAAAt                                          <  1:403019/92‑1 (MQ=255)
                                                     aCCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAAtt                                         >  1:101302/1‑100 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCATTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                       <  1:1522616/100‑1 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTa                                        <  1:552810/99‑1 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                       <  2:3403921/100‑1 (MQ=255)
                                                       cTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTAc                                       >  2:1540683/1‑100 (MQ=255)
                                                        ttCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACa                                      >  2:1923376/1‑100 (MQ=255)
                                                         tCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaa                                     <  1:2824368/100‑1 (MQ=255)
                                                         tCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACAATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaa                                     <  1:2238629/100‑1 (MQ=255)
                                                          cTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaat                                    >  1:1794508/1‑100 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGTTCACTTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                  >  1:1622848/1‑100 (MQ=255)
                                                            gacgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataa                                  >  2:1371981/1‑100 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATc                                <  2:3394563/100‑1 (MQ=255)
                                                              cgacTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataatn                                <  1:3057780/100‑2 (MQ=255)
                                                               gacTTTCTGCTTAAACAGAGTTCAATTTGATTATCACTATCCAAGCACTTACACCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACaataatn                                <  1:2960909/99‑2 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                             >  2:1023724/1‑100 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                             >  2:2836195/1‑100 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                             >  2:301191/1‑100 (MQ=255)
                                                                 cTTTCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTa                             >  1:1299935/1‑100 (MQ=255)
                                                                  tttCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGATAAATAATATTTAAAAAATTACAATAATCTTAt                            <  2:3300122/100‑1 (MQ=255)
                                                                   ttCTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATg                           <  2:546795/100‑1 (MQ=255)
                                                                   ttCTGCTTAAACAGGGATCAATTTGATTAACACAATACACGCAATTACACCAATTTTATTCATGGAAAAAAAAAAAAAAAAAAATTACAATAATCTTATg                           <  1:152414/100‑1 (MQ=255)
                                                                   cttccgatctaCAGGGTTCAATTTGATTATCAATATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATg                           <  1:2440733/90‑1 (MQ=255)
                                                                     cTGCTTAAACATGGTTCAATTTGATTATCACTATACACGTACTTACTACAATATTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTc                         <  1:2396928/100‑1 (MQ=255)
                                                                     cTGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTc                         >  2:2753638/1‑100 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATg                           >  1:2785792/1‑97 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCt                        >  2:1527380/1‑100 (MQ=255)
                                                                      tGCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCt                        >  1:251621/1‑100 (MQ=255)
                                                                       gCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTa                       <  2:1622227/100‑1 (MQ=255)
                                                                       gCTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAACAATATTTAAAAAATTACACTAATATTATGTCTa                       >  1:1290096/1‑100 (MQ=255)
                                                                        cTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTaa                      >  2:591949/1‑100 (MQ=255)
                                                                        cTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTaa                      >  2:3408367/1‑100 (MQ=255)
                                                                        cTTAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTaa                      >  1:1247074/1‑100 (MQ=255)
                                                                          tAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAAtt                    >  1:2616778/1‑100 (MQ=255)
                                                                          tAAACAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAAtt                    >  2:446040/1‑100 (MQ=255)
                                                                          tAAACAGAGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATTGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAAtt                    <  1:1593115/100‑1 (MQ=255)
                                                                           aaaCAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTg                   <  2:1082176/100‑1 (MQ=255)
                                                                           aaaCAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTg                   <  2:1341395/100‑1 (MQ=255)
                                                                            aaCAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTgg                  >  1:70397/1‑100 (MQ=255)
                                                                             aCAGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATgagatcggaag                 >  2:2440766/1‑90 (MQ=255)
                                                                               aGGGTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaac               <  2:3301756/100‑1 (MQ=255)
                                                                                  gTTCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacgga            >  1:2820925/1‑100 (MQ=255)
                                                                                   ttCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacggaa           <  2:2225895/100‑1 (MQ=255)
                                                                                    tCAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTggaacggaac          <  1:1215746/100‑1 (MQ=255)
                                                                                     cAATTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTTgaacggaacg         >  1:1017941/1‑100 (MQ=255)
                                                                                      aaTTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGAAACGGAACGc        >  1:608468/1‑100 (MQ=255)
                                                                                       aTTTGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCt       >  1:2328612/1‑100 (MQ=255)
                                                                                        tttGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCtt      >  2:1996010/1‑100 (MQ=255)
                                                                                          tGATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCtttt    <  2:2876716/100‑1 (MQ=255)
                                                                                           gATTATCACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAAAAAAAAATAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTg   <  1:2482645/100‑1 (MQ=255)
                                                                                            attatcACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGc  <  2:1196467/100‑1 (MQ=255)
                                                                                            attatcACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGc  <  2:1067194/100‑1 (MQ=255)
                                                                                                 |                                                                                              
ATAGTCAGAAGGTTAAGATCAATATTTCGTAAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTCTGCTTAAACAGGGTTCAATTTGATTATTACTATCCACGCACTTACTCCAATTTTATTCATGGAAAAATAATATTTAAAAAATTACAATAATCTTATGTCTAATTGGAACGGAACGCTTTTGC  >  NC_000913/661272‑661463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: