BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,636	T→G	T3P (ACC→CCC)	ybeD ←	UPF0250 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,636	0	T	G	99.3%	487.8 / ‑6.3	138	T3P (ACC→CCC)	ybeD	UPF0250 family protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (69/68); total (69/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 141 total aligned reads displayed.

ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATT  >  NC_000913/662539‑662730

aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgtt                                                                                              >  2:197376/1‑100 (MQ=255)

aCCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgtt                                                                                              >  2:2397232/1‑100 (MQ=255)

 ccACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGgttt                                                                                             >  2:2621847/1‑100 (MQ=255)

  cACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTc                                                                                            >  1:1329765/1‑100 (MQ=255)

  cACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTc                                                                                            <  2:2162263/100‑1 (MQ=255)

   aCTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                           >  1:3260776/1‑100 (MQ=255)

   aCTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                           <  1:993689/100‑1 (MQ=255)

   aCTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                           <  2:3230118/100‑1 (MQ=255)

   aCTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCa                                                                                           <  1:1119924/100‑1 (MQ=255)

    cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCAt                                                                                          >  2:2837380/1‑100 (MQ=255)

    cTTCAACCACCTGATCAACCAGCTCAGGCAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCAt                                                                                          >  2:2393327/1‑100 (MQ=255)

      tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGt                                                                                        >  2:2905692/1‑100 (MQ=255)

       cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTc                                                                                       <  1:1770524/100‑1 (MQ=255)

       cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTc                                                                                       >  1:2402933/1‑100 (MQ=255)

        aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCa                                                                                      <  1:1616987/100‑1 (MQ=255)

        aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCa                                                                                      <  2:3327003/100‑1 (MQ=255)

        aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCa                                                                                      <  2:1760099/100‑1 (MQ=255)

        aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCa                                                                                      >  1:86250/1‑100 (MQ=255)

         accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGt                                                                                        <  1:677672/97‑1 (MQ=255)

         accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAg                                                                                     >  1:3429156/1‑100 (MQ=255)

          ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGc                                                                                    >  2:77227/1‑100 (MQ=255)

            accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTc                                                                                  >  2:3232983/1‑100 (MQ=255)

            accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTc                                                                                  >  2:1011066/1‑100 (MQ=255)

             ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTGAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTcc                                                                                 >  1:185253/1‑100 (MQ=255)

               tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCgg                                                                               >  1:1790600/1‑100 (MQ=255)

               tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCgg                                                                               <  1:2687919/100‑1 (MQ=255)

                   cAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgta                                                                           >  1:2645510/1‑100 (MQ=255)

                   cAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgta                                                                           >  2:3377845/1‑100 (MQ=255)

                    aaCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaa                                                                          >  2:3090462/1‑100 (MQ=255)

                      ccAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacg                                                                        >  2:2451524/1‑100 (MQ=255)

                       cAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacgt                                                                       <  1:396870/100‑1 (MQ=255)

                        aGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGcgtaacgta                                                                      >  2:1603125/1‑100 (MQ=255)

                          cTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAAt                                                                    >  2:2207363/1‑100 (MQ=255)

                          cTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAAt                                                                    <  2:3007261/100‑1 (MQ=255)

                           tCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAAtt                                                                   <  2:2678843/100‑1 (MQ=255)

                             aGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTa                                                                  <  2:125501/99‑1 (MQ=255)

                              ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTaaa                                                                >  1:2681754/1‑100 (MQ=255)

                              ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGCTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTaaa                                                                <  1:1927030/100‑1 (MQ=255)

                               gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTaaan                                                               <  1:3090412/100‑2 (MQ=255)

                               gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAAt                                                               <  1:1658324/100‑1 (MQ=255)

                                tAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATa                                                              >  2:168844/1‑100 (MQ=255)

                                tAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATa                                                              <  2:2740678/100‑1 (MQ=255)

                                tAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATa                                                              <  1:1300031/100‑1 (MQ=255)

                                 aaCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATa                                                              >  1:1407525/1‑99 (MQ=255)

                                   cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCa                                                           >  1:510155/1‑100 (MQ=255)

                                    gCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCaa                                                          >  1:224457/1‑100 (MQ=255)

                                    gCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCaa                                                          >  2:2907677/1‑100 (MQ=255)

                                     ccTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAAc                                                         <  2:922429/100‑1 (MQ=255)

                                       tGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTc                                                       >  1:1707200/1‑100 (MQ=255)

                                          cccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                    <  2:958371/100‑1 (MQ=255)

                                          cccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                    >  1:2728816/1‑100 (MQ=255)

                                           ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGc                                                   >  2:1383074/1‑100 (MQ=255)

                                           ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGc                                                   >  1:825388/1‑100 (MQ=255)

                                           ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGGTTCATGTCAGATCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                    >  1:419830/1‑99 (MQ=255)

                                              tAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAg                                                <  1:2425122/100‑1 (MQ=255)

                                                aCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGccttct                                              <  2:1453315/100‑5 (MQ=255)

                                                     gtaagtaaAAGGAGTAGGGAATTCGAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCgg                                         <  2:390898/100‑1 (MQ=255)

                                                     gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCgg                                         <  1:978365/100‑1 (MQ=255)

                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                      <  2:641827/100‑1 (MQ=255)

                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                      <  2:3372441/100‑1 (MQ=255)

                                                        agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                      >  1:2849474/1‑100 (MQ=255)

                                                          taaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                      <  1:2629595/98‑1 (MQ=255)

                                                          taaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGt                                     >  1:1407246/1‑99 (MQ=255)

                                                           aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                   >  2:2257430/1‑100 (MQ=255)

                                                           aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                   >  2:817432/1‑100 (MQ=255)

                                                            aaaGGAGTAGGGAATTCAAGCAGTGCGTCAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTCAATAGCAACTCCCGCCTTCTGGCGGGAGttgc                                  <  1:2539907/100‑3 (MQ=37)

                                                            aaaGGAGTAGGGAATTCAAGCACTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttn                                  <  2:1494098/100‑2 (MQ=255)

                                                             cccttcagcagAATTCCAGCAGATCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                 >  1:185323/11‑100 (MQ=255)

                                                              aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                <  2:1055946/100‑1 (MQ=255)

                                                              aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                <  2:136982/100‑1 (MQ=255)

                                                               ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                               <  2:3059471/100‑1 (MQ=255)

                                                               ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                               >  2:803718/1‑100 (MQ=255)

                                                               ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                               >  1:3474903/1‑100 (MQ=255)

                                                               ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                               <  1:228630/100‑1 (MQ=255)

                                                               ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTGTTTTa                               >  2:2264649/1‑100 (MQ=255)

                                                                gAGTAGGTAATTCAAGCGGTGCGTTAAGTTTGGGTTTCATGTCAGCTGCGGCGTAACGTAATTAAATTGCAACTCCCGCCAGAAGGCGGGAGTTTTTTAc                              <  1:2591764/100‑1 (MQ=255)

                                                                  gTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTg                            <  2:1242461/100‑1 (MQ=255)

                                                                  gTAGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTg                            >  2:351422/1‑100 (MQ=255)

                                                                    aGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAt                          >  2:1918532/1‑100 (MQ=255)

                                                                    aGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAt                          >  2:1729510/1‑100 (MQ=255)

                                                                    aGGGAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTGTTTTACTGAt                          >  1:1667756/1‑100 (MQ=255)

                                                                     gggAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATg                         >  2:3000675/1‑100 (MQ=255)

                                                                     gggAATTCAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATg                         <  2:3170359/100‑1 (MQ=255)

                                                                       gAATTCAAGCAGTTCGTTAAGCTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCt                       <  1:224093/100‑1 (MQ=255)

                                                                            cAAGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGAGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                  <  1:421601/100‑1 (MQ=255)

                                                                             aaGCAGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtat                 <  2:1839253/100‑1 (MQ=255)

                                                                                 aGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgg             <  1:3164020/100‑1 (MQ=255)

                                                                                 aGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgg             <  2:516138/100‑1 (MQ=255)

                                                                                 aGTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCAGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgg             <  1:1114890/100‑1 (MQ=255)

                                                                                  gTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg            <  1:929959/100‑1 (MQ=255)

                                                                                  gTTCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg            <  1:1383054/100‑1 (MQ=255)

                                                                                    tCGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGa          <  1:2755963/100‑1 (MQ=255)

                                                                                     cGTTAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGAc         <  1:8574/100‑1 (MQ=255)

                                                                                       ttAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACgg       >  1:1713068/1‑100 (MQ=255)

                                                                                        tAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGa      >  2:1450351/1‑100 (MQ=255)

                                                                                        tAAGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGCTTTTTACTGATGCTTAGTATATGGGGACgg       >  1:2999833/1‑99 (MQ=255)

                                                                                          aGTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGaaa    >  2:1328463/1‑100 (MQ=255)

                                                                                           gTTTGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt   >  2:2646431/1‑100 (MQ=255)

                                                                                            tttGGTTTTCATGCCAGCCCCGGCGAAACGTATTAAAAAAGCACCCCCCCCAAGAAGGGGGAATTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt   <  1:3072712/99‑1 (MQ=255)

                                                                                            tttGGGTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAtt  <  1:1353399/100‑1 (MQ=255)

ACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATT  >  NC_000913/662539‑662730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`