Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,805	A→T	C264S (TGC→AGC)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,805	0	A	T	100.0%	320.8 / NA	95	C264S (TGC→AGC)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (58/37);  total (58/37)

TGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCC  >  NC_000913/700709‑700900                                                                                                 |                                                                                                tGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGcc                                                                                              >  2:2882851/1‑100 (MQ=255)   cAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCaa                                                                                            <  1:22313/100‑1 (MQ=255)   cAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCaa                                                                                            >  2:1285105/1‑100 (MQ=255)     gtcgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAg                                                                                          <  1:1380989/100‑1 (MQ=255)      tcgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGt                                                                                         <  2:1702859/100‑1 (MQ=255)       cgtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGtt                                                                                        >  2:2890978/1‑100 (MQ=255)       cgtcCAGCGGCACGCGGCTCTGGTAGACCTGCTTTAACAGCTTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGACAGTATCCACGCTGCCAAAGt                                                                                         >  1:1479450/1‑99 (MQ=255)        gtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTg                                                                                       <  1:1628958/100‑1 (MQ=255)        gtcCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTg                                                                                       <  1:262574/100‑1 (MQ=255)          ccAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGcc                                                                                     >  1:1766632/1‑100 (MQ=255)           cAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCg                                                                                    >  1:2779196/1‑100 (MQ=255)            aGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGc                                                                                   >  2:3442239/1‑100 (MQ=255)              cGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAg                                                                                 <  2:1465603/100‑1 (MQ=255)                gCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTg                                                                               >  2:3176054/1‑100 (MQ=255)                 cACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTgg                                                                              <  1:1286794/100‑1 (MQ=255)                 cACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTgg                                                                              >  1:656528/1‑100 (MQ=255)                 cACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTgg                                                                              >  1:3454834/1‑100 (MQ=255)                  aCGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGc                                                                             <  1:2083727/100‑1 (MQ=255)                  aCGCGGCTCGGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTGCCAGGCTGCCAAAGTTTCCGCAGTg                                                                               >  1:508012/1‑98 (MQ=255)                   cgcgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCa                                                                            >  2:1209226/1‑100 (MQ=255)                     cgGCTCTGGTAGCCCTGCTTTACCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATCGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGc                                                                          >  2:454706/1‑100 (MQ=255)                     cgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAg                                                                           <  1:3282199/99‑1 (MQ=255)                     cgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGc                                                                          >  2:1798514/1‑100 (MQ=255)                     cgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGc                                                                          >  2:2498599/1‑100 (MQ=255)                     cgGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGc                                                                          >  2:2997943/1‑100 (MQ=255)                      ggCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCg                                                                         <  2:2029885/100‑1 (MQ=255)                      ggCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCg                                                                         <  2:2714971/100‑1 (MQ=255)                      ggCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCg                                                                         <  2:135668/100‑1 (MQ=255)                      ggCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCg                                                                         >  1:3023802/1‑100 (MQ=255)                       gCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGt                                                                        <  1:23722/100‑1 (MQ=255)                       gCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGt                                                                        <  2:514134/100‑1 (MQ=255)                         tctGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTc                                                                      <  1:2199586/100‑1 (MQ=255)                              tAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCaac                                                                   >  1:3279907/1‑96 (MQ=255)                                gCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGc                                                               >  2:713617/1‑100 (MQ=255)                                  ccTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCgg                                                             >  2:17191/1‑100 (MQ=255)                                     gCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTc                                                          <  2:714355/100‑1 (MQ=255)                                     gCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTc                                                          <  1:1874927/100‑1 (MQ=255)                                      cTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGACAGCGTTCACCCAGCGGTTCg                                                         >  1:591141/1‑100 (MQ=255)                                         tatcAGATTCAGCTCCCGTTGTTCAATGTCAGTGTTGGCAGCGATAGTGTCCAGGATGCGAATGTTGCCGCAGTGGCAGCGTTAACCTAGCGGCTCGAcc                                                      <  1:2944620/97‑1 (MQ=255)                                         tAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACGCAGCGGTTCGAcc                                                      <  1:2154636/100‑1 (MQ=255)                                         tAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc                                                      <  2:2594351/100‑1 (MQ=255)                                          aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt                                                     >  2:1559023/1‑100 (MQ=255)                                          aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt                                                     >  2:779464/1‑100 (MQ=255)                                          aaCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCt                                                     >  1:1591061/1‑100 (MQ=255)                                           aCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc                                                      >  1:375978/1‑98 (MQ=255)                                           aCAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg                                                    <  2:3333584/100‑1 (MQ=255)                                            cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTg                                                    >  2:1251235/1‑99 (MQ=255)                                            cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa                                                   <  2:2831748/100‑1 (MQ=255)                                            cAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGa                                                   >  1:2584850/1‑100 (MQ=255)                                             aGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa                                                  <  1:1362086/100‑1 (MQ=255)                                             aGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa                                                  >  2:1362228/1‑100 (MQ=255)                                             aGATTCAACACCCGTTCTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa                                                  <  1:2038327/100‑1 (MQ=255)                                              gATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGaa                                                  >  1:1175557/1‑99 (MQ=255)                                              gATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAat                                                 >  2:2656929/1‑100 (MQ=255)                                               aTTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAata                                                <  1:1158033/100‑1 (MQ=255)                                               aTTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAata                                                >  2:2326265/1‑100 (MQ=255)                                                    acacCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGAAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGc                                            <  1:2617326/99‑1 (MQ=255)                                                      acCTGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCaa                                         >  1:2643049/1‑100 (MQ=255)                                                      acCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCaa                                         <  2:2845533/100‑1 (MQ=255)                                                       cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAt                                        >  1:1375399/1‑100 (MQ=255)                                                       cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAt                                        >  2:2030305/1‑100 (MQ=255)                                                       cccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAt                                        >  1:3126648/1‑100 (MQ=255)                                                        ccGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAtt                                       >  1:3477351/1‑100 (MQ=255)                                                         cGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAAttt                                      >  2:1551514/1‑100 (MQ=255)                                                          gttgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTc                                     <  2:3331648/100‑1 (MQ=255)                                                            tgttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAc                                   >  2:1738423/1‑100 (MQ=255)                                                             gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                  >  2:3401819/1‑100 (MQ=255)                                                             gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                  >  2:1679924/1‑100 (MQ=255)                                                             gttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCAcc                                  >  2:2567200/1‑100 (MQ=255)                                                              ttCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCg                                 >  1:1253071/1‑100 (MQ=255)                                                                  aTGGCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgt                             >  2:487292/1‑100 (MQ=255)                                                                   nggCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgtt                            >  2:452628/2‑100 (MQ=255)                                                                     gCAGCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgc                          <  2:2614360/100‑1 (MQ=255)                                                                        gCGTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccg                        <  2:2096850/99‑1 (MQ=255)                                                                          gTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgttg                     <  2:2829824/100‑1 (MQ=255)                                                                          gTTGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTACGACCTGGATATGGCCAATTTCACCGAcgttgccgttg                     >  1:1895193/1‑100 (MQ=255)                                                                           ttGGCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGAcgttgccgttgc                    <  2:352533/100‑1 (MQ=255)                                                                             ggCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCgg                  >  2:260227/1‑100 (MQ=255)                                                                             ggCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCgg                  >  2:3120379/1‑100 (MQ=255)                                                                             ggCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCgg                  >  1:2318635/1‑100 (MQ=255)                                                                             ggCAGCGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCgg                  >  2:1811131/1‑100 (MQ=255)                                                                                  cGATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCCAt             >  2:1352629/1‑100 (MQ=255)                                                                                   gATAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATa            >  1:1426944/1‑100 (MQ=255)                                                                                    aTAGTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaa           >  1:1641341/1‑100 (MQ=255)                                                                                       gTTTCCAGGCTGCCAAAGTTGCCGCAGTGTCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaa          <  1:1987372/98‑1 (MQ=255)                                                                                       gTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa        >  2:667837/1‑100 (MQ=255)                                                                                       gTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa        >  1:1145858/1‑100 (MQ=255)                                                                                       gTTTCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATaaaaa        >  1:3116094/1‑100 (MQ=255)                                                                                          tCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgc     >  1:1451328/1‑100 (MQ=255)                                                                                          tCCAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgc     >  1:460994/1‑100 (MQ=255)                                                                                            cAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcgc   >  2:442439/1‑100 (MQ=255)                                                                                            cAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcgc   <  1:383866/100‑1 (MQ=255)                                                                                            cAGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATgcg    <  1:965105/99‑1 (MQ=255)                                                                                             aGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGcc  <  2:3020293/100‑1 (MQ=255)                                                                                             aGGCTGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGcc  <  2:2266687/100‑1 (MQ=255)                                                                                                 |                                                                                                TGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACCTGAATATGGCCAATTTCACCGACGTTGCCGTTGCGGCCAATAAAAATGCGCC  >  NC_000913/700709‑700900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑