Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 834,352 A→T Q428L (CAG→CTG)  dinG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913834,3520AT97.5% 389.3 / ‑2.4 121Q428L (CAG→CTG) dinGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (56/61);  total (57/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCG  >  NC_000913/834255‑834447
                                                                                                 |                                                                                               
ttCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGc                                                                                               <  2:3279317/100‑1 (MQ=255)
ttCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGc                                                                                               <  2:1685409/100‑1 (MQ=255)
 tCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGgctgct                                                                                              >  2:3080301/1‑100 (MQ=255)
  cGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTa                                                                                             >  2:200536/1‑100 (MQ=255)
  cGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTa                                                                                             >  1:1431972/1‑100 (MQ=255)
   gAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTac                                                                                            <  2:1471321/100‑1 (MQ=255)
   gAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAAGCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGgctgct                                                                                              <  1:341216/98‑1 (MQ=255)
    aGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTaca                                                                                           >  1:1687143/1‑100 (MQ=255)
      gcgcAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACAcc                                                                                         <  1:2813260/100‑1 (MQ=255)
       cgcAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACct                                                                                        >  2:1787275/1‑100 (MQ=255)
        gcaaagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGACGAGCGCGAAGGGCTGCTACACctc                                                                                       >  1:967825/1‑100 (MQ=255)
        gcaaagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACctc                                                                                       <  1:523223/100‑1 (MQ=255)
           aagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTgg                                                                                    <  2:452002/100‑1 (MQ=255)
           aagcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTgg                                                                                    <  2:1487828/100‑1 (MQ=255)
            agcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGt                                                                                   >  2:835453/1‑100 (MQ=255)
            agcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGt                                                                                   >  2:2733897/1‑100 (MQ=255)
             gcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGTAAGAGCGCGAAGGGCTGCTACACCTCTGGtt                                                                                  <  2:2337896/100‑1 (MQ=255)
             gcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGtt                                                                                  <  2:482211/100‑1 (MQ=255)
             gcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGtt                                                                                  <  1:1886310/100‑1 (MQ=255)
             gcaaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGtt                                                                                  >  1:1133431/1‑100 (MQ=255)
              caaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGttt                                                                                 <  1:3296475/100‑1 (MQ=255)
              caaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGttt                                                                                 >  1:3094953/1‑100 (MQ=255)
               aaaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTc                                                                                <  2:2030391/100‑1 (MQ=255)
                aaCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCa                                                                               <  2:2809794/100‑1 (MQ=255)
                aaCTCTGGCGGCTGGCTTCGCTGGCACAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCa                                                                               >  1:511780/1‑100 (MQ=255)
                 aCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCAc                                                                              >  1:610958/1‑100 (MQ=255)
                 aCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCAc                                                                              <  2:2633654/100‑1 (MQ=255)
                 aCTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCAc                                                                              <  2:273779/100‑1 (MQ=255)
                  ctctGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACt                                                                             <  2:1805711/100‑1 (MQ=255)
                  ctctGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACt                                                                             >  1:839120/1‑100 (MQ=255)
                  ctctGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACt                                                                             <  2:3320019/100‑1 (MQ=255)
                  ctctGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGAGTGAAGGGCTGCTACACCTCTGGTTTCACt                                                                             >  1:1219559/1‑100 (MQ=255)
                    ctGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGc                                                                           >  2:3278417/1‑100 (MQ=255)
                    ctGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGc                                                                           >  1:1785774/1‑100 (MQ=255)
                    ctGGCGGCGGGCTACGCTGGCGCAATCCTCCGGTGGACCGGTGACCAAATGGGCGACGCTGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTCCACTg                                                                            <  1:1614608/99‑1 (MQ=255)
                      ggcggcGGGCTTCGCTGGCGCAAGCTGCCGGTGCACCGGTGACCAAATGGGCGACGCGGGATGATCGCGAAGGGCTGCTACACCTCTGGTTTCAc                                                                              <  1:2282810/95‑1 (MQ=255)
                        cggcTGGCTTCGCTGGCGCAATCTTCCGGTGCACCCGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTgg                                                                       <  1:2056622/100‑1 (MQ=255)
                         ggctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTggg                                                                      <  1:2614963/100‑1 (MQ=255)
                         ggctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTggg                                                                      <  2:2861051/100‑1 (MQ=255)
                         ggctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCGTggg                                                                      <  2:2662690/100‑1 (MQ=255)
                           ctggctTCGCTGGTGTAATCTTCCGGTGCACCGGAGACCAAATGGGCGGCCCGGGAAGAGCGCGAAGGGCTGCGACACCGCTGGTTTCACTGCGTGGGaa                                                                    <  1:1765001/100‑1 (MQ=255)
                           ctggctTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGaa                                                                    <  1:2583028/100‑1 (MQ=255)
                                ttCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACgt                                                               >  2:1071530/1‑100 (MQ=255)
                                  cGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACgtgt                                                             >  1:1419279/1‑100 (MQ=255)
                                    cTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTc                                                            >  1:2122409/1‑99 (MQ=255)
                                      ggCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGCAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTACGTGGGAATACGTGTCAGc                                                         >  1:3367293/1‑100 (MQ=255)
                                      ggCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGc                                                         >  2:3432145/1‑100 (MQ=255)
                                        cgcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGa                                                       >  2:2417253/1‑100 (MQ=255)
                                        cgcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGa                                                       >  1:250013/1‑100 (MQ=255)
                                        cgcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGa                                                       >  2:1714657/1‑100 (MQ=255)
                                         gtaaTCTTCCGGTGCCCCGGTGGCCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTTTGGTTTTACTGCGTGGGAATACGTGTCAg                                                          <  1:890222/94‑1 (MQ=255)
                                         gcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGAt                                                      <  1:2733854/100‑1 (MQ=255)
                                         gcAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGAt                                                      <  1:1185824/100‑1 (MQ=255)
                                          cAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCgct                                                      >  1:2070085/1‑97 (MQ=255)
                                          cAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATc                                                     <  1:1229052/100‑1 (MQ=255)
                                             tCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGc                                                  <  2:2312377/100‑1 (MQ=255)
                                               ttCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTg                                                <  2:1980569/100‑1 (MQ=255)
                                                 ccGGTGCACCGGTGACCAAATGGTCGACGCGGGAATAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTgg                                               <  2:1574107/99‑1 (MQ=255)
                                                   ggTGCACCGGTGACCAAATGGGCGACGCAGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGaaa                                            <  2:2180667/100‑1 (MQ=255)
                                                     tGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAgg                                          <  2:1994188/100‑1 (MQ=255)
                                                      gCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgc                                         <  1:1034763/100‑1 (MQ=255)
                                                       cACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgct                                        >  1:3003533/1‑100 (MQ=255)
                                                         ccGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGAGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGgctgc                                      >  1:1853120/1‑100 (MQ=255)
                                                           ggTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGctgctg                                    >  2:2528799/1‑100 (MQ=255)
                                                           ggTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGctgctg                                    >  1:2193658/1‑100 (MQ=255)
                                                            gTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCtgt                                   <  1:861082/100‑1 (MQ=255)
                                                             tGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCtgtg                                  <  1:2417220/100‑1 (MQ=255)
                                                              gACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTgg                                 <  2:3284498/100‑1 (MQ=255)
                                                              gACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTgg                                 <  2:3138689/100‑1 (MQ=255)
                                                              gACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTgg                                 <  1:2975935/100‑1 (MQ=255)
                                                               aCCAAATGGGCGACGCGGGAAGTGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGgc                                <  1:1719164/100‑1 (MQ=255)
                                                               aCCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTgg                                 <  2:418535/99‑1 (MQ=255)
                                                               aCCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGgc                                >  2:1002940/1‑100 (MQ=255)
                                                               aCCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGgc                                <  1:143808/100‑1 (MQ=255)
                                                                 cAAATGGGCGACGCGGGAAGAGCGCGTAGGGCTGCTACGCCTCTGGTTTCGCTGCGTGGGAATGCGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGgcgc                              <  1:330914/100‑1 (MQ=255)
                                                                  aaaTGGGCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCa                             >  1:189968/1‑100 (MQ=255)
                                                                    aTGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGCTCAGCTGGAAAGGCTGCTGTGGCGCAGt                           >  1:825445/1‑100 (MQ=255)
                                                                      gggCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTAt                         >  1:2951672/1‑100 (MQ=255)
                                                                       ggCGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTAtt                        >  1:2736452/1‑100 (MQ=255)
                                                                         cGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTcc                      <  2:511786/100‑1 (MQ=255)
                                                                         cGACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTcc                      <  2:3302777/100‑1 (MQ=255)
                                                                          gACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCATTATTCCg                     <  2:1821095/100‑1 (MQ=255)
                                                                          gACGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCg                     >  1:2499863/1‑100 (MQ=255)
                                                                           aCGCGGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGc                    <  2:1094797/100‑1 (MQ=255)
                                                                            cgcgGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGca                   >  1:1730691/1‑100 (MQ=255)
                                                                             gcgGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGcac                  <  1:2364839/100‑1 (MQ=255)
                                                                              cgGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGcaca                 >  2:1958072/1‑100 (MQ=255)
                                                                              cgGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGcaca                 >  1:3362637/1‑100 (MQ=255)
                                                                              cgGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGcaca                 >  2:2566161/1‑100 (MQ=255)
                                                                              cgGGAAGAGCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGcaca                 <  2:1246209/100‑1 (MQ=255)
                                                                              cgGGAAGAGCCCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATTCGTGTCACCTATCAGCTGGAAAGGCTGCTGTGGCTCAGTATTCCGc                    <  1:1932219/97‑1 (MQ=255)
                                                                                    gagCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTg           >  2:2047949/1‑100 (MQ=255)
                                                                                    gagCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTg           >  2:3089590/1‑100 (MQ=255)
                                                                                    gagCGCGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTg           >  2:2652528/1‑100 (MQ=255)
                                                                                     agCGCGAAGGGCTGCTACACCTCTGGTTTCACTGTGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGt          >  1:3089563/1‑100 (MQ=255)
                                                                                      gcgcGAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTc         <  2:2314359/100‑1 (MQ=255)
                                                                                       cgcgAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCa        >  2:736080/1‑100 (MQ=255)
                                                                                         cgAAGGGCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCAcc      >  2:309204/1‑100 (MQ=255)
                                                                                             gggCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCg  <  2:204497/100‑1 (MQ=255)
                                                                                             gggCTGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCg  <  1:2528765/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TTCGAGGCGCAAAGCAAACTCTGGCGGCTGGCTTCGCTGGCGCAATCTTCCGGTGCACCGGTGACCAAATGGGCGACGCGGGAAGAGCGCGAAGGGCAGCTACACCTCTGGTTTCACTGCGTGGGAATACGTGTCAGCGATCAGCTGGAAAGGCTGCTGTGGCGCAGTATTCCGCACATTATTGTCACCTCCG  >  NC_000913/834255‑834447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑