BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	894,155	T→G	D124E (GAT→GAG)	potF →	putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	894,155	0	T	G	100.0%	431.8 / NA	122	D124E (GAT→GAG)	potF	putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (64/58); total (64/58)

Only 100 of 122 total aligned reads displayed.

TGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCC  >  NC_000913/894061‑894251

 gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGAc                                                                                            <  1:374486/100‑1 (MQ=255)

     gCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATa                                                                                        >  2:214190/1‑100 (MQ=255)

     gCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATa                                                                                        >  2:2891365/1‑100 (MQ=255)

      cGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATaa                                                                                       >  1:1348095/1‑100 (MQ=255)

      cGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATaa                                                                                       >  2:2821255/1‑100 (MQ=255)

       gggAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATaa                                                                                       >  2:2370587/1‑99 (MQ=255)

           gTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTg                                                                                  >  2:2512096/1‑100 (MQ=255)

             tttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCt                                                                                >  1:242131/1‑100 (MQ=255)

                ccAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATg                                                                             <  1:1095114/100‑1 (MQ=255)

                ccAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACCAGCCCGACAATAAATTTGCTAt                                                                              >  1:1047772/1‑99 (MQ=255)

                 cAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTAtcc                                                                            <  2:2338112/100‑3 (MQ=255)

                      gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCtat                                                                       <  2:121777/100‑1 (MQ=255)

                      gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCtat                                                                       >  1:1242875/1‑100 (MQ=255)

                      gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCta                                                                        >  1:2313355/1‑99 (MQ=255)

                       cTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCtata                                                                      <  2:803363/100‑1 (MQ=255)

                       cTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCtata                                                                      <  1:570264/100‑1 (MQ=255)

                         ggACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                    <  2:2545000/100‑1 (MQ=255)

                           aCAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                  >  2:616486/1‑100 (MQ=255)

                           aCAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                  <  1:1984508/100‑1 (MQ=255)

                           aCAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                  <  1:1772117/100‑1 (MQ=255)

                            cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTgg                                                                 <  2:1398798/100‑1 (MQ=255)

                            cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTgg                                                                 >  1:1274105/1‑100 (MQ=255)

                             aaaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTggg                                                                <  1:3194133/100‑1 (MQ=255)

                             aaaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTggg                                                                <  2:1059550/100‑1 (MQ=255)

                              aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               >  2:1585627/1‑100 (MQ=255)

                              aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               >  1:3073313/1‑100 (MQ=255)

                              aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               >  1:1051833/1‑100 (MQ=255)

                               aaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGGAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcg                                                              <  1:2913497/100‑1 (MQ=255)

                               aaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcg                                                              <  2:1126766/100‑1 (MQ=255)

                                aGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGACCTATATGTGGGcga                                                             >  2:3427998/1‑100 (MQ=255)

                                 gCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcgac                                                            >  2:2229625/1‑100 (MQ=255)

                                   aaaTTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcgacga                                                          <  1:3427946/100‑1 (MQ=255)

                                   aaaTTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcgacga                                                          >  2:1791240/1‑100 (MQ=255)

                                    aaTTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCgacgac                                                         >  2:1063825/1‑100 (MQ=255)

                                    aaTTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCgacgac                                                         >  2:32223/1‑100 (MQ=255)

                                        gCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCggg                                                     <  1:2300684/100‑1 (MQ=255)

                                        gCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCggg                                                     >  2:1905431/1‑100 (MQ=255)

                                            gAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTg                                                 <  2:29811/100‑1 (MQ=255)

                                            gAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTg                                                 <  1:1857712/100‑1 (MQ=255)

                                              gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGc                                               >  1:1207989/1‑100 (MQ=255)

                                              gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAACAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGc                                               <  1:1497093/100‑1 (MQ=255)

                                               tGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                              >  2:3400520/1‑100 (MQ=255)

                                                ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                             <  2:2436766/100‑1 (MQ=255)

                                                ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                             >  1:3068672/1‑100 (MQ=255)

                                                ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                             >  2:3035153/1‑100 (MQ=255)

                                                 gaagaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCtat                                            >  1:761870/1‑100 (MQ=255)

                                                  aagaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCtata                                           <  1:335494/100‑1 (MQ=255)

                                                   agaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATaa                                          >  2:266827/1‑100 (MQ=255)

                                                   agaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATaa                                          <  1:2485762/100‑1 (MQ=255)

                                                    tccgatctaCCCGGAACTGCTGAATCTGGTCGCCGAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAc                                         <  1:1832431/92‑1 (MQ=255)

                                                     aaTCTCGACCCGGAACTTCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACg                                        <  1:2725020/100‑1 (MQ=255)

                                                     aaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACg                                        >  2:2479194/1‑100 (MQ=255)

                                                     aaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACg                                        >  1:3156113/1‑100 (MQ=255)

                                                     aaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACg                                        >  2:1778610/1‑100 (MQ=255)

                                                       tctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGtt                                      >  2:2163049/1‑100 (MQ=255)

                                                       tctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGtt                                      >  2:2823750/1‑100 (MQ=255)

                                                       tctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGtt                                      >  1:1015872/1‑100 (MQ=255)

                                                        ctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTg                                     >  2:927339/1‑100 (MQ=255)

                                                        ctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTg                                     <  1:2635836/100‑1 (MQ=255)

                                                          cGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGAt                                   >  2:2105109/1‑100 (MQ=255)

                                                          cGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGAt                                   >  1:3314407/1‑100 (MQ=255)

                                                           gACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATa                                  >  1:2014668/1‑100 (MQ=255)

                                                            aCCCGGAGCTGCTGAATCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAcagatcgga                                 >  2:1832456/1‑92 (MQ=255)

                                                            aCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATaa                                 >  2:342523/1‑100 (MQ=255)

                                                            aCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATaa                                 >  2:2630187/1‑100 (MQ=255)

                                                             cccGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATaaa                                >  2:1190228/1‑100 (MQ=255)

                                                              ccGTAACTGCGGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATGTGCTATGCCTGATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAg                               <  1:3034328/100‑1 (MQ=255)

                                                              ccGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAg                               <  2:1247331/100‑1 (MQ=255)

                                                               cGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGt                              <  2:1195996/100‑1 (MQ=255)

                                                                  aaCTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTaa                           <  2:2412111/100‑1 (MQ=255)

                                                                   aCTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTaaa                          <  2:2178623/100‑1 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  1:640526/1‑100 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  2:1334663/1‑100 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       <  1:335994/100‑1 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  1:197361/1‑100 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  2:344430/1‑100 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  2:187870/1‑100 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  2:155571/1‑100 (MQ=255)

                                                                      gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  2:1319865/1‑100 (MQ=255)

                                                                       ctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCgg                      >  2:379456/1‑100 (MQ=255)

                                                                        tgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCGATGCCCTAGAGGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGGTAAAGCGGt                     >  2:2771082/1‑100 (MQ=255)

                                                                         gAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTg                    >  2:1842181/1‑100 (MQ=255)

                                                                          aaGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGc                   <  1:101960/100‑1 (MQ=255)

                                                                             cTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTgg                <  1:897529/100‑1 (MQ=255)

                                                                               ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc              >  1:2126542/1‑100 (MQ=255)

                                                                                  cGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTGTAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaa           >  1:1833163/1‑100 (MQ=255)

                                                                                  cGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaa           >  1:781643/1‑100 (MQ=255)

                                                                                    ccAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaaaa         >  2:1695171/1‑100 (MQ=255)

                                                                                     cAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAc        >  1:2241661/1‑100 (MQ=255)

                                                                                     cAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATGGGCTATAACGTTGATAAAGTTAAAGCGGGGCTGGGCgcgtgt        <  2:2352822/100‑6 (MQ=255)

                                                                                      aaaCACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcg       <  2:100968/100‑1 (MQ=255)

                                                                                       aaCACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcgc      <  1:2516970/100‑1 (MQ=255)

                                                                                       aaCACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcgc      <  2:1648082/100‑1 (MQ=255)

                                                                                        acacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcgcg     <  1:2585115/100‑1 (MQ=255)

                                                                                        acacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcgcg     <  1:267104/100‑1 (MQ=255)

                                                                                        acacGAGCCCGACAAGAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcgcg     <  2:3426669/100‑1 (MQ=255)

                                                                                         cacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGATAACgcgc    <  2:1833188/100‑1 (MQ=255)

                                                                                         cacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACgcgc    <  1:1842156/100‑1 (MQ=255)

                                                                                           cGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGcc   <  1:2350774/99‑1 (MQ=255)

                                                                                           cGAGCCCGACAAGAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTAGAACGTTAATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGccc  >  1:2434437/1‑100 (MQ=255)

TGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCC  >  NC_000913/894061‑894251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`