Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 905,720 C→A L270M (CTG→ATG)  amiD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913905,7200CA98.3% 382.8 / NA 115L270M (CTG→ATG) amiD1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent; OM lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (56/57);  total (58/57)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGC  >  NC_000913/905623‑905815
                                                                                                 |                                                                                               
gcagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATg                                                                                               >  2:1555738/1‑100 (MQ=255)
gcagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATg                                                                                               <  1:1513743/100‑1 (MQ=255)
  agcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGga                                                                                             <  2:2073823/100‑1 (MQ=255)
   gcggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGgag                                                                                            <  1:2020591/100‑1 (MQ=255)
     ggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGaa                                                                                          <  1:3168006/100‑1 (MQ=255)
     ggcgCGAGAGTATGGCAGCCGAGATGCATTTCCGCCCGACGTTATATAACGGCGAGGCGGATGCAGAAACACAGGCGATTGCCGAAGCATTCCTGgag                                                                                            >  1:2775673/1‑98 (MQ=255)
       cgcgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAAt                                                                                        <  2:2178054/100‑1 (MQ=255)
       cgcgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAAt                                                                                        <  2:2976457/100‑1 (MQ=255)
        gcgTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATa                                                                                       <  2:150367/100‑1 (MQ=255)
           tGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACgg                                                                                    <  1:1677576/100‑1 (MQ=255)
           tGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACgg                                                                                    <  2:2389528/100‑1 (MQ=255)
           tGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACgg                                                                                    <  2:2076959/100‑1 (MQ=255)
              ttATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCa                                                                                 <  2:3212150/100‑1 (MQ=255)
              ttATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCa                                                                                 <  2:809690/100‑1 (MQ=255)
                aTGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAgg                                                                               <  2:1210444/100‑1 (MQ=255)
                aTGGCATTCCAGATGCATTTCCGCCCGACGGTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAAGACGGGCAgg                                                                               >  2:696380/1‑100 (MQ=255)
                   gCATTCCCGATGCATTTCCGCCCGACGTTATATAATGGCGAAGCGGAAGCCGGAACGCAGGCGATTGCTGAAACCGTGATGGAGGACTACGGGCAGGAt                                                                             >  1:280244/1‑99 (MQ=255)
                   gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATATGGGCAGGAtt                                                                            <  1:1149864/100‑1 (MQ=255)
                   gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAtt                                                                            <  1:1807108/100‑1 (MQ=255)
                   gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAtt                                                                            <  1:1066547/100‑1 (MQ=255)
                   gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAtt                                                                            <  1:1784493/100‑1 (MQ=255)
                   gCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGAtt                                                                            >  2:492204/1‑100 (MQ=255)
                        ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAgcgc                                                                       <  1:37216/100‑1 (MQ=255)
                        ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAgcgc                                                                       <  1:924459/100‑1 (MQ=255)
                         cAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGcgcg                                                                      >  2:279469/1‑100 (MQ=255)
                          aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCgg                                                                     <  2:1324151/100‑1 (MQ=255)
                          aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCgg                                                                     >  2:2014953/1‑100 (MQ=255)
                          aGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCCACGCACTGATGGAGAAATACGGGCAGGATTAGcgcg                                                                      >  1:2343051/1‑99 (MQ=255)
                             tGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAg                                                                  >  1:95553/1‑100 (MQ=255)
                             tGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAg                                                                  >  2:1799497/1‑100 (MQ=255)
                              gCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGt                                                                 >  2:2177128/1‑100 (MQ=255)
                                  ttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTc                                                             <  2:1319110/100‑1 (MQ=255)
                                   tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                            <  2:2855824/100‑1 (MQ=255)
                                   tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                            >  1:810464/1‑100 (MQ=255)
                                   tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                            >  1:2079666/1‑100 (MQ=255)
                                    ccGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCg                                                           <  1:171366/100‑1 (MQ=255)
                                     cGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGt                                                          >  1:521434/1‑100 (MQ=255)
                                      ncccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                         >  2:190877/2‑100 (MQ=255)
                                      gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGGAGGATTAGCGCGGCAGTTTTCCGTg                                                         >  1:1792007/1‑100 (MQ=255)
                                      gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTg                                                         <  1:1625337/100‑1 (MQ=255)
                                      gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTGTTCCGTg                                                         >  2:1590175/1‑100 (MQ=255)
                                        ccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGt                                                       <  1:3258941/100‑1 (MQ=255)
                                         cGACGTTATCTAACGGAGAAGCGAAGGCAGAAACTCAGGCGATTGCCGAAGCAATGATGTAAAAAAACGTGAAGGATTAGCGCGGCAGCTTgtcgtcgtc                                                      >  1:518646/1‑100 (MQ=255)
                                         cGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGt                                                       <  1:2192955/99‑1 (MQ=255)
                                          gACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcg                                                     <  2:2060104/100‑1 (MQ=255)
                                          gACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcg                                                     <  1:635426/100‑1 (MQ=255)
                                              ttATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTa                                                 >  2:1690887/1‑100 (MQ=255)
                                               tatataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAg                                                >  2:3452411/1‑100 (MQ=255)
                                               tatataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAg                                                <  2:909832/100‑1 (MQ=255)
                                                atataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTCGCGCGGCAGTTTTCCGTGGTCGCGTAGc                                               >  1:1488124/1‑100 (MQ=255)
                                                atataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGc                                               >  1:2393854/1‑100 (MQ=255)
                                                 tataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGcc                                              <  2:2309657/100‑1 (MQ=255)
                                                   taACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAg                                            >  2:8131/1‑100 (MQ=255)
                                                   taACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAg                                            >  1:503521/1‑100 (MQ=255)
                                                    aaCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                           >  1:1388202/1‑100 (MQ=255)
                                                     aCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggc                                          >  1:2717365/1‑100 (MQ=255)
                                                      cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcg                                         >  1:2189574/1‑100 (MQ=255)
                                                       ggCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcgg                                        >  1:1118723/1‑100 (MQ=255)
                                                        gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcggc                                       <  1:3166530/100‑1 (MQ=255)
                                                               ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                <  1:985252/100‑1 (MQ=255)
                                                               ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                <  2:1363208/100‑1 (MQ=255)
                                                               ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATAAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                >  1:116575/1‑100 (MQ=255)
                                                               atctGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                <  1:572755/97‑1 (MQ=255)
                                                                gATGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtct                               >  1:2695459/1‑100 (MQ=255)
                                                                 aTGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtctc                              <  1:8131/100‑1 (MQ=255)
                                                                  tGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg                             <  1:179452/100‑1 (MQ=255)
                                                                  tGCAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCg                             <  1:358495/100‑1 (MQ=255)
                                                                    cAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt                           >  1:564361/1‑100 (MQ=255)
                                                                    cAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt                           >  1:2289397/1‑100 (MQ=255)
                                                                    cAGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGAt                           >  2:222535/1‑100 (MQ=255)
                                                                     aGAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATa                          <  2:3232904/100‑1 (MQ=255)
                                                                      gAAACTCAGGCGATTGCCGAAGCATTGATGGAGGAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAc                         <  1:403985/100‑1 (MQ=255)
                                                                      gAAACTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAc                         >  1:733190/1‑100 (MQ=255)
                                                                       aaaCTCAGGCGATTGCCGAAGCATTGATGGAGAAATATGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                        <  2:1664543/100‑1 (MQ=255)
                                                                       aaaCTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                        <  1:1555716/100‑1 (MQ=255)
                                                                         aCTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCtt                      <  2:1723449/100‑1 (MQ=255)
                                                                         aCTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCtt                      <  2:2910608/100‑1 (MQ=255)
                                                                          cTCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc                     >  2:3431782/1‑100 (MQ=255)
                                                                           tCAGGCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCa                    >  1:2494220/1‑100 (MQ=255)
                                                                              ggagATTGCCGAAGCATTGCTGGAGCAATACGGGAAGGATTAGCGCGGCAGTTTTCCGTGGTCTCGTAGCCAGGCGTCAGGTTTCTCGATACCTCCATc                  >  1:2377802/4‑99 (MQ=255)
                                                                               gCGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCa                <  1:3289936/100‑1 (MQ=255)
                                                                                cGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAg               >  1:596885/1‑100 (MQ=255)
                                                                                cGATTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAg               <  2:317709/100‑1 (MQ=255)
                                                                                  aTTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAggg             <  2:2393887/100‑1 (MQ=255)
                                                                                  aTTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAggg             <  2:2600052/100‑1 (MQ=255)
                                                                                  aTTGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAggg             <  2:733200/100‑1 (MQ=255)
                                                                                   ttGCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGt            <  2:3036127/100‑1 (MQ=255)
                                                                                     gCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga          >  2:2721/1‑100 (MQ=255)
                                                                                     gCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga          <  2:1728250/100‑1 (MQ=255)
                                                                                     gCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtga          >  2:705936/1‑100 (MQ=255)
                                                                                     gCCGAAGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGATTTCTCGATACCTTCATCCGGGGtga          >  1:1695051/1‑100 (MQ=255)
                                                                                       cGAAGCATTGATGGAGAAATACGGGAAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatg        >  1:695377/1‑100 (MQ=255)
                                                                                        gAAGCATTGATGGAGAAATAGGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga       <  1:2908334/100‑1 (MQ=255)
                                                                                         aaGCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGAGGCAGTTTTCTCGATACGTTCATCCAGGGTGATGAc      >  1:1545187/1‑100 (MQ=255)
                                                                                           gCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCg    >  2:2418419/1‑100 (MQ=255)
                                                                                           gCATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCg    <  2:3237371/100‑1 (MQ=255)
                                                                                            cATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg   >  1:1229030/1‑100 (MQ=255)
                                                                                            cATTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCgg   <  2:2716368/100‑1 (MQ=255)
                                                                                             aTTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGc  <  2:1787787/100‑1 (MQ=255)
                                                                                             aTTGATGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGc  <  1:3422344/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGC  >  NC_000913/905623‑905815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: