Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 931,952 C→T V33V (GTG→GTA trxB ← thioredoxin reductase, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913931,9520CT98.4% 397.0 / ‑2.8 123V33V (GTG→GTAtrxBthioredoxin reductase, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (62/58);  total (63/60)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 125 total aligned reads displayed.

TTAATAACGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCC  >  NC_000913/931855‑932047
                                                                                                 |                                                                                               
ttAATAACGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGtac                                                                                               <  1:2734346/100‑1 (MQ=255)
 taataaCGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGtaca                                                                                              <  2:2069164/100‑1 (MQ=255)
       cGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggttgc                                                                                        <  1:1427698/100‑1 (MQ=255)
        ggACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggttgca                                                                                       <  2:1869238/100‑1 (MQ=255)
         gACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggttgcag                                                                                      <  1:3199391/100‑1 (MQ=255)
           ccGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggttgcaggt                                                                                    >  1:216814/1‑100 (MQ=255)
           ccGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggttgcaggt                                                                                    >  2:1745950/1‑100 (MQ=255)
            cGGTCAGCTCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTAcaggttgcaggtt                                                                                   >  2:1823423/1‑100 (MQ=255)
              gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTgg                                                                                 >  2:792279/1‑100 (MQ=255)
              gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTgg                                                                                 >  2:2897283/1‑100 (MQ=255)
              gTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTgg                                                                                 <  2:59498/100‑1 (MQ=255)
                 aGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGgcgc                                                                              >  1:963195/1‑100 (MQ=255)
                  gATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGgcgcg                                                                             >  1:451568/1‑100 (MQ=255)
                    tCGTTTGGATCGCCAGGACAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCGGGTAATCAGTACAGGTTGCAGGTTGgcgcg                                                                             >  1:753148/1‑98 (MQ=255)
                     cGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGgcgcgcgc                                                                          <  1:2526113/100‑1 (MQ=255)
                     cGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGgcgcgcgc                                                                          <  1:2526106/100‑1 (MQ=255)
                     cGTTTGGAGCGCTAGGCCAGATTTCAACTTTCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGAAGGTTGgcgcgcgc                                                                          >  1:948018/1‑100 (MQ=255)
                       tttGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGcgccg                                                                        >  2:2628223/1‑100 (MQ=255)
                         tGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCcgcg                                                                      >  2:395166/1‑100 (MQ=255)
                         tGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCcgcg                                                                      >  1:68078/1‑100 (MQ=255)
                           gATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTa                                                                    >  2:1485124/1‑100 (MQ=255)
                           gATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTa                                                                    >  1:1870983/1‑100 (MQ=255)
                            aTCGCCAGGCCAGTTGTCCACTTCCGTGGTGGTGGGCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCCGGTTGGCGCGCGCCGCGTAg                                                                   <  1:2119626/100‑1 (MQ=255)
                             tCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGa                                                                  >  1:619740/1‑100 (MQ=255)
                              cGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGGAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAc                                                                 >  1:1222051/1‑100 (MQ=255)
                               gccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAca                                                                >  1:3111550/1‑100 (MQ=255)
                               gccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAca                                                                <  2:366270/100‑1 (MQ=255)
                               gccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAca                                                                >  1:2666001/1‑100 (MQ=255)
                                ccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcag                                                               >  2:1233930/1‑100 (MQ=255)
                                ccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcag                                                               <  1:2538019/100‑1 (MQ=255)
                                ccaggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcag                                                               >  2:247681/1‑100 (MQ=255)
                                 caggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagc                                                              <  2:1605681/100‑1 (MQ=255)
                                 caggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagc                                                              <  2:3089202/100‑1 (MQ=255)
                                   ggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAccgcag                                                            >  2:518060/1‑100 (MQ=255)
                                   ggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagcag                                                            >  1:3055578/1‑100 (MQ=255)
                                   ggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagcag                                                            >  1:1625585/1‑100 (MQ=255)
                                   ggccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGAcagcag                                                            >  2:550295/1‑100 (MQ=255)
                                    gccagTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACagcagc                                                           >  2:2590243/1‑100 (MQ=255)
                                        gTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGgtg                                                       <  2:2666040/100‑1 (MQ=255)
                                        gTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGgtg                                                       <  2:486362/100‑1 (MQ=255)
                                          tttCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTa                                                     <  1:2685896/100‑1 (MQ=255)
                                          tttCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTa                                                     >  2:337440/1‑100 (MQ=255)
                                           ttCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTAt                                                    >  2:1696522/1‑100 (MQ=255)
                                            tCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATc                                                   >  2:399234/1‑100 (MQ=255)
                                             ccACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTTCTTTCCGGTTATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATcc                                                  >  1:748251/1‑100 (MQ=255)
                                              cACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGAACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATcc                                                  >  2:107819/1‑99 (MQ=255)
                                                cTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCccgc                                               <  2:68079/100‑1 (MQ=255)
                                                  tCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCccgccg                                             >  2:1087648/1‑100 (MQ=255)
                                                   ccGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCgg                                            >  1:2359583/1‑100 (MQ=255)
                                                     gtggtggtgGTCAGTTGGCCACCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGc                                          <  1:2448076/100‑1 (MQ=255)
                                                      tggtggtggTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGcc                                         <  2:574045/100‑1 (MQ=255)
                                                      tggtggtggTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGcc                                         <  2:3160101/100‑1 (MQ=255)
                                                      tggtggtggTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGcc                                         <  2:2893456/100‑1 (MQ=255)
                                                       ggtggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGcc                                         <  2:1518431/99‑1 (MQ=255)
                                                       ggtggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCt                                        <  1:2158113/100‑1 (MQ=255)
                                                       ggtggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCt                                        >  1:2794288/1‑100 (MQ=255)
                                                         tggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGa                                      <  2:462131/100‑1 (MQ=255)
                                                          ggtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGaa                                     >  2:151755/1‑100 (MQ=255)
                                                           gtggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAc                                    <  1:2611234/100‑1 (MQ=255)
                                                            tggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAcc                                   <  2:963207/100‑1 (MQ=255)
                                                            tggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAcc                                   >  1:3393542/1‑100 (MQ=255)
                                                            tggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAcc                                   >  1:3399220/1‑100 (MQ=255)
                                                             ggtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAAccc                                  >  1:1130944/1‑100 (MQ=255)
                                                              gtCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCTGCCGGGCCTGAACCCa                                 >  2:553372/1‑100 (MQ=255)
                                                               tCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAg                                <  1:494417/100‑1 (MQ=255)
                                                               tCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAg                                <  1:2569289/100‑1 (MQ=255)
                                                               tCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAg                                <  1:1811227/100‑1 (MQ=255)
                                                               tCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAg                                >  1:3152400/1‑100 (MQ=255)
                                                                cAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAgg                               <  1:1598392/100‑1 (MQ=255)
                                                                cAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAgg                               <  2:2133920/100‑1 (MQ=255)
                                                                   ttGGCCGCCTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATa                            <  2:910680/100‑1 (MQ=255)
                                                                      gccgccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGc                         >  1:3449457/1‑100 (MQ=255)
                                                                       ccgccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCa                        >  2:3321063/1‑100 (MQ=255)
                                                                       ccgccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCa                        <  1:2590208/100‑1 (MQ=255)
                                                                       ccgccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCa                        >  1:1607727/1‑100 (MQ=255)
                                                                         gccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGt                      <  2:1309842/100‑1 (MQ=255)
                                                                         gccTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGt                      <  2:3252982/100‑1 (MQ=255)
                                                                           cttccgatctGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGttt                    <  1:2013438/91‑1 (MQ=255)
                                                                           cTTTTTCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGttt                    <  1:3450299/100‑1 (MQ=255)
                                                                            tttttCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATTCCGCCGGGCCTGAACCCAGGATAAGCAGTTTa                   >  1:231215/1‑100 (MQ=255)
                                                                            tttttCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTa                   <  1:579423/100‑1 (MQ=255)
                                                                             ttttCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTAc                  <  1:1552459/100‑1 (MQ=255)
                                                                               ttCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACtg                <  2:3055628/100‑1 (MQ=255)
                                                                               ttCCATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACtg                <  1:1814830/100‑1 (MQ=255)
                                                                                 ccATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACtgtg              <  2:243712/100‑1 (MQ=255)
                                                                                 ccATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACtgtg              >  1:2388194/1‑100 (MQ=255)
                                                                                 ccATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACtgtg              >  2:419814/1‑100 (MQ=255)
                                                                                 ccATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGAAGTTTACtgtg              >  1:787945/1‑100 (MQ=255)
                                                                                  cATGCCGTTAACCAGTACAGGTTGCAGATTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACtg                >  1:1821554/1‑97 (MQ=255)
                                                                                  cATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTgtgt             <  1:2559180/100‑1 (MQ=255)
                                                                                  cATGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTgtgt             >  2:2998242/1‑100 (MQ=255)
                                                                                    tGCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGttt           >  1:2252164/1‑100 (MQ=255)
                                                                                     gCCGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTagatcggaag          >  2:2013467/1‑91 (MQ=255)
                                                                                      ccGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTgg         >  2:3046370/1‑100 (MQ=255)
                                                                                      ccGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTgg         <  1:1233915/100‑1 (MQ=255)
                                                                                       cGGTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGt        <  2:1429909/100‑1 (MQ=255)
                                                                                         gTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCg      <  2:3191668/100‑1 (MQ=255)
                                                                                         gTAATCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCg      <  2:184480/100‑1 (MQ=255)
                                                                                           aaTCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTg    <  2:1424976/100‑1 (MQ=255)
                                                                                             tCAGTACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGcc  <  2:2096173/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TTAATAACGGACCGGTCAGATCGTTTGGATCGCCAGGCCAGTTTTCCACTTCCGTGGTGGTGGTCAGTTGGCCGCCTTTTTCCATGCCGGTAATCAGCACAGGTTGCAGGTTGGCGCGCGCCGCGTAGACAGCAGCGGTGTATCCCGCCGGGCCTGAACCCAGGATAAGCAGTTTACTGTGTTTGGTCGTGCC  >  NC_000913/931855‑932047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: