Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 975,083 T→A D255E (GAT→GAA)  mukF → chromosome condensin MukBEF, kleisin‑like subunit, binds calcium

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913975,0830TA97.8% 459.7 / 0.1 136D255E (GAT→GAA) mukFchromosome condensin MukBEF, kleisin‑like subunit, binds calcium
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base A (56/77);  total (57/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

Only 100 of 136 total aligned reads displayed.

AAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACC  >  NC_000913/974986‑975179
                                                                                                 |                                                                                                
aaGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACt                                                                                                <  2:614536/100‑1 (MQ=255)
aaGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACt                                                                                                <  2:3431754/100‑1 (MQ=255)
  gCGGCAGGCGACACATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGc                                                                                              <  2:2124722/100‑1 (MQ=255)
  gCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGc                                                                                              <  2:1978876/100‑1 (MQ=255)
   cGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCa                                                                                             <  2:2668725/100‑1 (MQ=255)
    ggCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCag                                                                                            <  1:1537119/100‑1 (MQ=255)
    ggCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCag                                                                                            >  1:1549376/1‑100 (MQ=255)
    ggCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCag                                                                                            <  2:513316/100‑1 (MQ=255)
     gCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCag                                                                                            >  2:1455303/1‑99 (MQ=255)
       aGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGc                                                                                         <  1:582431/100‑1 (MQ=255)
       aGGAGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCagag                                                                                          <  1:879756/99‑1 (MQ=255)
        ggCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCa                                                                                        <  1:520413/100‑1 (MQ=255)
         gCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCaa                                                                                       >  2:1051221/1‑100 (MQ=255)
           gACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAAc                                                                                     <  2:2939844/100‑1 (MQ=255)
           gACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTAGATCGTATGGTGTTCGAACTGCAGAGCaaa                                                                                      >  2:2977908/1‑99 (MQ=255)
                aTTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGAt                                                                                <  1:237317/100‑1 (MQ=255)
                  tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCGATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCg                                                                              <  2:3433427/100‑1 (MQ=255)
                  tGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCg                                                                              >  2:3155277/1‑100 (MQ=255)
                   gCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCg                                                                              <  1:2167380/99‑1 (MQ=255)
                    cAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGta                                                                            <  2:1771089/100‑1 (MQ=255)
                      ggCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatt                                                                          <  1:1358519/100‑1 (MQ=255)
                      ggCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatt                                                                          >  2:3246854/1‑100 (MQ=255)
                      ggCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatt                                                                          <  2:2183384/100‑1 (MQ=255)
                        cTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtatta                                                                         <  2:3317516/99‑1 (MQ=255)
                         tAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGtattaac                                                                       <  2:2348535/100‑3 (MQ=255)
                         tAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATc                                                                       <  2:3227390/100‑1 (MQ=255)
                           agcTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAg                                                                     >  2:2346221/3‑100 (MQ=255)
                                ttGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTggg                                                                <  2:2708523/100‑1 (MQ=255)
                                 tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                               >  1:2622106/1‑100 (MQ=255)
                                 tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                               >  2:3003088/1‑100 (MQ=255)
                                 tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                               >  2:283449/1‑100 (MQ=255)
                                 tGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTgggg                                                               >  2:323799/1‑100 (MQ=255)
                                   cgcATTCAGGATGCGACGATGACCCATGACGATCTGTATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGcc                                                             <  2:2441902/100‑1 (MQ=255)
                                    gcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCa                                                            <  2:2884356/100‑1 (MQ=255)
                                    gcATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCa                                                            <  1:2195170/100‑1 (MQ=255)
                                     cATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAg                                                           >  1:1644316/1‑100 (MQ=255)
                                      aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                          <  2:2663059/100‑1 (MQ=255)
                                      aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                          <  1:2693194/100‑1 (MQ=255)
                                      aTTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGc                                                          <  2:1678588/100‑1 (MQ=255)
                                         cAGGATGCGACGATGACCCATGACGATCTGAATATCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGAGGCCAGCAAt                                                       >  1:2282779/1‑100 (MQ=255)
                                          aGGATGCGACGATGACCCATGACGCTCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATc                                                      <  1:1819994/100‑1 (MQ=255)
                                          aGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATc                                                      <  1:1994640/100‑1 (MQ=255)
                                           ggATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATcc                                                     >  2:159820/1‑100 (MQ=255)
                                            gATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCa                                                    >  2:15445/1‑100 (MQ=255)
                                            gATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCa                                                    >  1:2448772/1‑100 (MQ=255)
                                               gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCacaa                                                 >  1:3018549/1‑97 (MQ=255)
                                               gCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCg                                                 <  2:963483/100‑1 (MQ=255)
                                                 gacgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGAc                                               >  1:2841577/1‑100 (MQ=255)
                                                  acgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACt                                              <  1:2649464/100‑1 (MQ=255)
                                                  acgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCACAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACt                                              <  1:1926567/100‑1 (MQ=255)
                                                   cgaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATAAATCGACtt                                             >  1:2351903/1‑100 (MQ=255)
                                                    gaTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtg                                            <  2:1769119/100‑1 (MQ=255)
                                                     aTGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgt                                           <  2:3424446/100‑1 (MQ=255)
                                                      tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATTAGTTGGGGCCAGCAATCCATCGACTtgtg                                          <  2:1864425/100‑1 (MQ=255)
                                                      tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGTAATCCATCGACTtgtg                                          <  1:823547/100‑1 (MQ=255)
                                                      tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgtg                                          <  2:1427732/100‑1 (MQ=255)
                                                      tGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTtgtg                                          <  2:2106802/100‑1 (MQ=255)
                                                        aCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGa                                        <  1:3426784/100‑1 (MQ=255)
                                                        aCCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTACGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGa                                        >  1:1849562/1‑100 (MQ=255)
                                                         cccATGACGGTCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGa                                        <  2:1146681/99‑1 (MQ=255)
                                                         cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                       <  1:690204/100‑1 (MQ=255)
                                                         cccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAt                                       >  1:1500749/1‑100 (MQ=255)
                                                          ccATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGAtt                                      >  1:381120/1‑100 (MQ=255)
                                                           cATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTg                                     >  1:574069/1‑100 (MQ=255)
                                                            aTGCCGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTgg                                    <  1:2305227/100‑1 (MQ=255)
                                                             tGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGc                                   <  2:748209/100‑1 (MQ=255)
                                                              gACGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCt                                  <  1:464038/100‑1 (MQ=255)
                                                                cGATTTGCATTTCGTCGATCGTCTGGAGTTCGAACTGCAGACCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTa                                 <  1:1543803/99‑1 (MQ=255)
                                                                cGATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTAc                                <  1:2416272/100‑1 (MQ=255)
                                                                 gATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACg                               <  1:2110438/100‑1 (MQ=255)
                                                                 gATCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACg                               >  1:1806870/1‑100 (MQ=255)
                                                                   tCTGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAc                             <  2:30856/100‑1 (MQ=255)
                                                                     tGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGAcc                            >  1:2268/1‑99 (MQ=255)
                                                                     tGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCg                           >  2:3398347/1‑100 (MQ=255)
                                                                     tGCATTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCg                           <  2:1354578/100‑1 (MQ=255)
                                                                        aTTTCGTCGATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCGATCGACGTGTGGATTGGCTAGGACCGCCa                        >  1:3030898/1‑100 (MQ=255)
                                                                           tcgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGt                     <  1:1051208/100‑1 (MQ=255)
                                                                            cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                    <  2:1717938/100‑1 (MQ=255)
                                                                            cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                    <  1:3246802/100‑1 (MQ=255)
                                                                            cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                    >  1:3042565/1‑100 (MQ=255)
                                                                            cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                    >  2:3462925/1‑100 (MQ=255)
                                                                            cgtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTa                    <  1:161716/100‑1 (MQ=255)
                                                                             gtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTac                   >  1:1013544/1‑100 (MQ=255)
                                                                             gtcgATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTac                   >  1:1173999/1‑100 (MQ=255)
                                                                                gATCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTAcaca                <  2:3266276/100‑1 (MQ=255)
                                                                                 aTCGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACaa               >  1:1660399/1‑100 (MQ=255)
                                                                                   cGTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAt             >  1:2317777/1‑100 (MQ=255)
                                                                                    gTCTGGTGTTCGAACTGCAGAGCAACCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTAGGACCGCCACGTACACAAAtt            >  1:895823/1‑100 (MQ=255)
                                                                                    gTCTGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAAtt            <  2:1218878/100‑1 (MQ=255)
                                                                                       tGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAt         >  2:3143441/1‑100 (MQ=255)
                                                                                       tGGTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAt         <  1:379922/100‑1 (MQ=255)
                                                                                        ggTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAtt        <  2:97917/100‑1 (MQ=255)
                                                                                        ggTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAtt        <  1:1308644/100‑1 (MQ=255)
                                                                                        ggTGTTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAtt        <  1:283445/100‑1 (MQ=255)
                                                                                        ggTGCTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTAtt        <  2:767931/100‑1 (MQ=255)
                                                                                           gtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt     >  2:3422677/1‑100 (MQ=255)
                                                                                           gtTCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGt     >  1:816608/1‑100 (MQ=255)
                                                                                             tCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAc   <  1:2811987/100‑1 (MQ=255)
                                                                                             tCGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAc   <  2:713513/100‑1 (MQ=255)
                                                                                              cGAACTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTAcc  <  2:530343/100‑1 (MQ=255)
                                                                                                 |                                                                                                
AAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACGATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACC  >  NC_000913/974986‑975179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑