Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 129,360 G→T intergenic (+24/+47) lpd → / ← yacH dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913129,3600GT97.1% 455.0 / NA 140intergenic (+24/+47)lpd/yacHdihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase/DUF3300 family protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  major base T (64/70);  minor base A (3/1);  total (69/71)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTG  >  NC_000913/129263‑129456
                                                                                                  |                                                                                                
cTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCa                                                                                                <  1:640081/100‑1 (MQ=255)
cTGTGGGCCTGGCGGCAGAAGTGTGCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCa                                                                                                <  2:2773166/100‑1 (MQ=255)
 tgtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCaa                                                                                               >  2:3147805/1‑100 (MQ=255)
 tgtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCaa                                                                                               <  2:2690052/100‑1 (MQ=255)
  gtgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAt                                                                                              <  2:2653521/100‑1 (MQ=255)
   tgGGCCTGGCGGTAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAtt                                                                                             >  2:3119581/1‑100 (MQ=255)
   tgGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAAtt                                                                                             >  2:1481692/1‑100 (MQ=255)
    gggCCTGGCGGCCGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTa                                                                                            <  1:2325271/100‑1 (MQ=255)
     ggCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCAGGACCATCCCTCAATTaa                                                                                           >  2:637925/1‑100 (MQ=255)
       ccTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaa                                                                                         >  2:1493319/1‑100 (MQ=255)
        cTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTaaaaa                                                                                        >  1:1941545/1‑100 (MQ=255)
          ggcggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTGAATTgaaaa                                                                                        >  1:1736862/1‑98 (MQ=255)
          ggcggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAg                                                                                      >  2:3277073/1‑100 (MQ=255)
            cggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCg                                                                                    <  2:3068093/100‑1 (MQ=255)
             ggcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCgg                                                                                   >  1:2191944/1‑100 (MQ=255)
              gcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGc                                                                                  <  1:3252326/100‑1 (MQ=255)
              gcAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTATCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGc                                                                                  <  2:2448233/100‑1 (MQ=255)
               cAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAAGCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCt                                                                                 >  1:1850809/1‑100 (MQ=255)
                aGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTa                                                                                <  1:1674354/100‑1 (MQ=255)
                  aaGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAAc                                                                              >  2:1314749/1‑100 (MQ=255)
                   aGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAAcc                                                                             >  1:1094142/1‑100 (MQ=255)
                   aGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAAcc                                                                             >  2:2640035/1‑100 (MQ=255)
                   aGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAAcc                                                                             >  1:465472/1‑100 (MQ=255)
                   aGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAAcc                                                                             <  1:452132/100‑1 (MQ=255)
                   aGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAAcc                                                                             >  1:1473222/1‑100 (MQ=255)
                      gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcg                                                                          >  1:540131/1‑100 (MQ=255)
                      gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcg                                                                          >  2:1157710/1‑100 (MQ=255)
                      gtTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcg                                                                          <  1:895901/100‑1 (MQ=255)
                       ttCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAc                                                                           <  1:624288/99‑1 (MQ=255)
                       ttCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGcgtggttagc                                                                         <  1:1189528/100‑10 (MQ=255)
                       ttCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgc                                                                         >  2:2565538/1‑100 (MQ=255)
                       ttCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgc                                                                         >  2:3468289/1‑100 (MQ=255)
                       ttCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgc                                                                         >  2:1653968/1‑100 (MQ=255)
                       ttCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgc                                                                         >  2:1625586/1‑100 (MQ=255)
                        tCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgcc                                                                        >  2:2436748/1‑100 (MQ=255)
                         cGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgccg                                                                       >  1:192370/1‑100 (MQ=255)
                          gAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCAcgccgc                                                                      >  2:2165732/1‑100 (MQ=255)
                           aaGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCt                                                                     <  2:2732524/100‑1 (MQ=255)
                            aGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGTCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtt                                                                    >  1:2432556/1‑100 (MQ=255)
                            aGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtt                                                                    <  2:3404080/100‑1 (MQ=255)
                              gTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCtttt                                                                  >  1:2863283/1‑100 (MQ=255)
                                 gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttttt                                                               <  1:900551/100‑1 (MQ=255)
                                 gCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCttttttt                                                               <  2:1831431/100‑1 (MQ=255)
                                  cATTACCGACCTGCCGAGCCCGAAAGCGAAGAAGCAGGAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                              <  2:621834/100‑1 (MQ=255)
                                  cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                              <  2:2625226/100‑1 (MQ=255)
                                  cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                              <  2:3205519/100‑1 (MQ=255)
                                  cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                              <  2:451004/100‑1 (MQ=255)
                                  cATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa                                                              <  2:615810/100‑1 (MQ=255)
                                   aTTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTAc                                                             <  1:3178937/100‑1 (MQ=255)
                                    ttACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACg                                                            <  1:2785044/100‑1 (MQ=255)
                                      atcGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc                                                          <  1:2101879/98‑1 (MQ=255)
                                        cGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTg                                                        <  2:2211377/100‑1 (MQ=255)
                                         gACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc                                                       >  2:2438713/1‑100 (MQ=255)
                                              gCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAAttt                                                  >  2:2621287/1‑100 (MQ=255)
                                               ccGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACAACGCCGCTTTTTTTACGTCTGCAAt                                                    >  1:87460/1‑97 (MQ=255)
                                                cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                >  2:150460/1‑100 (MQ=255)
                                                cGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAc                                                >  1:2041859/1‑100 (MQ=255)
                                                    cccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCttt                                            >  2:802102/1‑100 (MQ=255)
                                                     ccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTc                                           >  2:2829125/1‑100 (MQ=255)
                                                     ccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTc                                           <  2:2530021/100‑1 (MQ=255)
                                                     ccGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTc                                           >  1:1529541/1‑100 (MQ=255)
                                                      cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTAccagatc                                          >  2:1878343/1‑95 (MQ=255)
                                                      cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                          <  1:1242336/100‑1 (MQ=255)
                                                      cGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTcc                                          >  2:1005437/1‑100 (MQ=255)
                                                       gAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCa                                         <  2:3398613/100‑1 (MQ=255)
                                                        aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                        <  2:3023866/100‑1 (MQ=255)
                                                        aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                        <  1:2616985/100‑1 (MQ=255)
                                                        aaaGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAg                                        <  1:456614/100‑1 (MQ=255)
                                                          aGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtc                                      >  1:3265238/1‑100 (MQ=255)
                                                            cGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtctt                                    >  2:2308393/1‑100 (MQ=255)
                                                            cGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtctt                                    >  2:1301607/1‑100 (MQ=255)
                                                              aagaagaagTAATTTTTTTTTTTTTTTTACATCCGACAATTAAAAAAGCAGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                  >  1:712650/1‑100 (MQ=255)
                                                              aagaagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                  <  1:2882690/100‑1 (MQ=255)
                                                              aagaagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                  >  2:3191255/1‑100 (MQ=255)
                                                              aagaagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGtcttct                                  <  2:2143043/100‑1 (MQ=255)
                                                                 aagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGc                               <  1:802091/100‑1 (MQ=255)
                                                                 aagaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGc                               <  2:3425159/100‑1 (MQ=255)
                                                                  agaagTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCt                              >  2:740737/1‑100 (MQ=255)
                                                                     agTAATTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCa                           >  1:1603998/1‑100 (MQ=255)
                                                                       tAATTTTACGTATGCCGGAACATCCGACAATTAAAAAAGAGGCTAACCACGCCACATTTATTACGTCTGCAATTGACATTTCCAGTCTACTTGTACCACg                         >  1:1411418/1‑100 (MQ=255)
                                                                         aTTTTTCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGtt                       >  1:3187936/1‑100 (MQ=255)
                                                                            ttttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCa                     >  1:2895462/1‑100 (MQ=255)
                                                                             tttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCag                    <  2:3004124/100‑1 (MQ=255)
                                                                             tttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCag                    <  2:1993844/100‑1 (MQ=255)
                                                                              ttCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCaga                   <  1:822739/100‑1 (MQ=255)
                                                                               tCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCggag                  <  1:3220724/100‑1 (MQ=255)
                                                                               tCGTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagag                  <  2:2322862/100‑1 (MQ=255)
                                                                                 gTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagagag                <  2:540137/100‑1 (MQ=255)
                                                                                 gTTTGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCagagag                >  1:2709212/1‑100 (MQ=255)
                                                                                  tttGCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAgagaga               <  1:1314730/100‑1 (MQ=255)
                                                                                     gCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt            >  1:15537/1‑100 (MQ=255)
                                                                                     gCCGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGt            >  2:600532/1‑100 (MQ=255)
                                                                                      ccGGAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGtt           >  1:1446534/1‑100 (MQ=255)
                                                                                        ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg         >  1:1686985/1‑100 (MQ=255)
                                                                                        ggAACATCCGTCAATTAAAAAAGCGGCTAACCACGCCCCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCg         <  2:2076176/100‑1 (MQ=255)
                                                                                         gAACATCCGTCAATTAGAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCGTTCCATTCTTCTTGCTCCACGTTCAGAGAGACGTTCg         <  2:3318099/99‑1 (MQ=255)
                                                                                          aaCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGc        <  2:1155576/99‑1 (MQ=255)
                                                                                          aaCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCa       <  2:3324045/100‑1 (MQ=255)
                                                                                           aCATCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCAt      <  1:1036823/100‑1 (MQ=255)
                                                                                              tCCGTCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATAct   <  2:177103/100‑1 (MQ=255)
                                                                                                  |                                                                                                
CTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTG  >  NC_000913/129263‑129456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: