Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,017,296 A→C I335S (ATC→AGC)  ycbZ ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,017,2960AC99.2% 453.5 / ‑4.2 129I335S (ATC→AGC) ycbZputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (55/73);  total (55/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 131 total aligned reads displayed.

AAAGCGCGTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGATCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCATCAC  >  NC_000913/1017200‑1017392
                                                                                                |                                                                                                
aaaGCGGGTGGATGACCCGGAAATTCGATGACCGAAAGGGCGTTAAGTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGctct                                                                                               <  1:274089/100‑1 (MQ=255)
aaaGCGCGTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGctct                                                                                               <  2:1312375/100‑1 (MQ=255)
   gcgcGTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCAt                                                                                            <  2:585473/100‑1 (MQ=255)
     gcgTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATcc                                                                                          >  1:2735395/1‑100 (MQ=255)
       gTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCATGATTTGCTCCTGAAGGCTCTCATCCTg                                                                                        <  2:2037090/100‑1 (MQ=255)
       gTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTg                                                                                        <  1:2621693/100‑1 (MQ=255)
       gTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTg                                                                                        <  1:3100174/100‑1 (MQ=255)
        tGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGc                                                                                       <  1:732098/100‑1 (MQ=255)
        tGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGc                                                                                       <  2:3367187/100‑1 (MQ=255)
         ggATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGc                                                                                       <  2:1611780/99‑1 (MQ=255)
         ggATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCa                                                                                      <  2:1393885/100‑1 (MQ=255)
          gATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCAt                                                                                     <  2:2846618/100‑1 (MQ=255)
          gATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCAt                                                                                     <  1:2355467/100‑1 (MQ=255)
           aTGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATa                                                                                    >  1:2251732/1‑100 (MQ=255)
            tGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATAc                                                                                   <  1:1795882/100‑1 (MQ=255)
               cttccgatctCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGtt                                                                                <  2:904708/91‑1 (MQ=255)
               cccGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGtt                                                                                >  2:1903297/1‑100 (MQ=255)
               cccGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGtt                                                                                <  1:2472893/100‑1 (MQ=255)
                ccGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTc                                                                               >  2:2368473/1‑100 (MQ=255)
                ccGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTc                                                                               >  2:2235447/1‑100 (MQ=255)
                ccGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTc                                                                               >  1:219009/1‑100 (MQ=255)
                 cGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCATGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCa                                                                              <  1:1052783/100‑1 (MQ=255)
                 cGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCa                                                                              >  2:3035597/1‑100 (MQ=255)
                    aaaTTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCg                                                                           <  2:329234/100‑1 (MQ=255)
                     aaTTCAATGGCCGGTAGGGCGTTACTTTGCCCTCTGCGTTCGCCTTCGGTTTCGCTCAGGATTTGCTCCTGAAGGCGCTCCTCCTGCATACGTTcg                                                                              <  1:3334957/96‑2 (MQ=255)
                      aTTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAg                                                                         >  2:2353535/1‑100 (MQ=255)
                      aTTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAg                                                                         >  2:542760/1‑100 (MQ=255)
                       ttCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAgg                                                                        >  1:454693/1‑100 (MQ=255)
                        tCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAgg                                                                        <  1:1018059/99‑1 (MQ=255)
                         cAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGttagacggaaga                                                                      >  1:904696/1‑90 (MQ=255)
                         cAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGaa                                                                      >  1:2660393/1‑100 (MQ=255)
                         cAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGaa                                                                      >  2:1451935/1‑100 (MQ=255)
                          aaTGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGaaa                                                                     >  1:3458377/1‑100 (MQ=255)
                           aTGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGATCTCATCCTGCATACGTTCAGCGAGGAAAc                                                                    <  2:2979351/100‑1 (MQ=255)
                              aCCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCtt                                                                 >  1:1473861/1‑100 (MQ=255)
                              aCCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCtt                                                                 >  1:776663/1‑100 (MQ=255)
                               ccGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTc                                                                >  2:1020969/1‑100 (MQ=255)
                                cGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTcg                                                               <  1:419843/100‑1 (MQ=255)
                                 gAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTcgc                                                              >  1:475666/1‑100 (MQ=255)
                                 gAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTcgc                                                              >  2:3397925/1‑100 (MQ=255)
                                 gAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTcgc                                                              <  1:635575/100‑1 (MQ=255)
                                  aaaGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTcgcg                                                             >  2:3020675/1‑100 (MQ=255)
                                    aGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGCAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGcc                                                           >  1:2221543/1‑100 (MQ=255)
                                        cGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTc                                                       <  1:3035548/100‑1 (MQ=255)
                                        cGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTc                                                       >  2:3202465/1‑100 (MQ=255)
                                        cGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTc                                                       <  2:366483/100‑1 (MQ=255)
                                         gTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTc                                                       >  2:1542417/1‑99 (MQ=255)
                                         gTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCa                                                      >  2:1322843/1‑100 (MQ=255)
                                          ttAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCAc                                                     <  2:664530/100‑1 (MQ=255)
                                          ttAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCAc                                                     <  2:1280344/100‑1 (MQ=255)
                                           tAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACg                                                    <  2:3286224/100‑1 (MQ=255)
                                              tttGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACgctg                                                 <  1:2679131/100‑1 (MQ=255)
                                              tttGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACgctg                                                 >  2:2837613/1‑100 (MQ=255)
                                              tttGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACgctg                                                 <  2:382997/100‑1 (MQ=255)
                                                 gCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGctgctg                                              >  1:3184906/1‑100 (MQ=255)
                                                   ccGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTgca                                            <  2:1021897/100‑1 (MQ=255)
                                                    cGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTgcag                                           <  2:1729013/100‑1 (MQ=255)
                                                       tGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCAt                                        <  2:2536451/100‑1 (MQ=255)
                                                         cGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTa                                      <  1:1511507/100‑1 (MQ=255)
                                                          gTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTaa                                     >  1:512115/1‑100 (MQ=255)
                                                          gTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTaa                                     >  2:565953/1‑100 (MQ=255)
                                                            tCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGt                                   <  1:3397873/100‑1 (MQ=255)
                                                            tCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGt                                   <  2:1377164/100‑1 (MQ=255)
                                                              gCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGttt                                 <  2:1544166/100‑1 (MQ=255)
                                                              gCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGttt                                 <  2:1774369/100‑1 (MQ=255)
                                                              gCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGttt                                 <  2:1020107/100‑1 (MQ=255)
                                                              gCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGttt                                 <  1:3020626/100‑1 (MQ=255)
                                                               ccTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTa                                <  2:920881/100‑1 (MQ=255)
                                                                cTTCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAg                               <  1:782448/100‑1 (MQ=255)
                                                                 ttCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAgc                              <  2:306008/100‑1 (MQ=255)
                                                                  tCGGTTTCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAgct                             <  2:1633298/100‑1 (MQ=255)
                                                                       ttCAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCg                        <  1:2319191/100‑1 (MQ=255)
                                                                         cAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGcc                      >  2:2271460/1‑100 (MQ=255)
                                                                         cAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGcc                      >  1:2272150/1‑100 (MQ=255)
                                                                         cAATCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGcc                      >  1:2259171/1‑100 (MQ=255)
                                                                          aaTCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCg                     >  1:3407109/1‑100 (MQ=255)
                                                                          aaTCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCg                     <  2:660042/100‑1 (MQ=255)
                                                                          aaTCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCg                     >  1:186513/1‑100 (MQ=255)
                                                                           aTCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCgg                    <  2:249839/100‑1 (MQ=255)
                                                                           aTCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCgg                    <  2:703257/100‑1 (MQ=255)
                                                                            tCAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGga                   >  1:2815974/1‑100 (MQ=255)
                                                                            ncAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGga                   >  2:2217991/2‑100 (MQ=255)
                                                                             cAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGgag                  <  2:1265036/100‑1 (MQ=255)
                                                                             cAGGATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGgag                  >  1:1562092/1‑100 (MQ=255)
                                                                                gATTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAg               <  1:2200126/100‑1 (MQ=255)
                                                                                 aTTTGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAgg              <  2:2964583/100‑1 (MQ=255)
                                                                                   ttGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGgtg            <  1:3042172/100‑1 (MQ=255)
                                                                                    tGCTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGgtg            >  1:1769310/1‑99 (MQ=255)
                                                                                      cTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCg         <  2:1546296/100‑1 (MQ=255)
                                                                                      cTCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCg         <  1:2461605/100‑1 (MQ=255)
                                                                                       tCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCg         >  1:1013988/1‑99 (MQ=255)
                                                                                       tCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGc        >  1:661548/1‑100 (MQ=255)
                                                                                       tCCTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGc        >  1:2831714/1‑100 (MQ=255)
                                                                                         cTGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCGCGCCGGAGAAGGTGTCGCCa      <  2:1825788/100‑1 (MQ=255)
                                                                                          tGAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCAt     <  2:2780917/100‑1 (MQ=255)
                                                                                          tGAAGGCTCGCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCAt     <  1:1010173/100‑1 (MQ=255)
                                                                                           gAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCATc    <  2:482347/100‑1 (MQ=255)
                                                                                           gAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCATc    >  2:1199516/1‑100 (MQ=255)
                                                                                           gAAGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCATc    <  2:1993964/100‑1 (MQ=255)
                                                                                             aGGCTCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCATcac  <  2:884957/100‑1 (MQ=255)
                                                                                                |                                                                                                
AAAGCGCGTGGATGACCCGGAAATTCAATGACCGAAAGGGCGTTAATTTGCCCGATGCGTTCGCCTTCGGTTTCAATCAGGATTTGCTCCTGAAGGATCTCATCCTGCATACGTTCAGCGAGGAAACCTTCGCGCCATTCACGCTGCTGCAGCATTAAGTTTAGCTGCTCGCCGGAGAAGGTGTCGCCATCAC  >  NC_000913/1017200‑1017392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: