Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,197,739 A→G S167P (TCC→CCC)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,197,7390AG99.3% 492.1 / ‑4.8 145S167P (TCC→CCC) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (77/67);  total (77/68)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 168 total aligned reads displayed.

ACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTAC  >  NC_000913/1197642‑1197835
                                                                                                 |                                                                                                
actGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGgaa                                                                                                >  1:437618/1‑100 (MQ=255)
  tGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAAtt                                                                                              >  1:358157/1‑100 (MQ=255)
  tGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAAtt                                                                                              <  2:378054/100‑1 (MQ=255)
  tGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAAtt                                                                                              <  2:3444452/100‑1 (MQ=255)
    agaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTa                                                                                             <  1:1005052/99‑1 (MQ=255)
      aaTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTaa                                                                                            <  1:3282903/98‑1 (MQ=255)
      aaTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATg                                                                                          <  2:1382934/100‑1 (MQ=255)
       ataaTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAATATGAAAAATATCCATTTTTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                         <  1:796808/97‑1 (MQ=255)
       aTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                         <  2:1944589/100‑1 (MQ=255)
         gATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCaa                                                                                       >  1:3019276/1‑100 (MQ=255)
          aTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCAATTTAAAAAAAAAAAAAGATTATTAGGAAGGGAATTAATGCAAt                                                                                      <  1:2765716/100‑1 (MQ=255)
          aTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATAACCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCaa                                                                                       >  1:1818855/1‑99 (MQ=255)
           tAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAAtt                                                                                     <  2:2090580/100‑1 (MQ=255)
               aTTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCg                                                                                 <  2:293574/100‑1 (MQ=255)
               aTTACAAAAAAGAATAAAAATAGCAAAGAACCTACAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCg                                                                                 <  1:920191/100‑1 (MQ=255)
                   cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa                                                                             >  1:618045/1‑100 (MQ=255)
                   cAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaaaa                                                                             >  1:65136/1‑100 (MQ=255)
                    aaaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATTCAATTATAGAAAAt                                                                            >  1:155873/1‑100 (MQ=255)
                     aaaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAAtt                                                                           >  2:1356594/1‑100 (MQ=255)
                      aaaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTc                                                                          >  2:1388518/1‑100 (MQ=255)
                       aaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCa                                                                         >  2:2228025/1‑100 (MQ=255)
                         aGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                       <  2:1183340/100‑1 (MQ=255)
                             tAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAat                                                                   >  1:2013540/1‑100 (MQ=255)
                               aaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAatat                                                                 >  2:689016/1‑100 (MQ=255)
                               aaGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAatat                                                                 >  2:282602/1‑100 (MQ=255)
                                 gACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATcc                                                               >  1:2352768/1‑100 (MQ=255)
                                 gACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATcc                                                               <  1:2686998/100‑1 (MQ=255)
                                  aCAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCa                                                              >  1:1996905/1‑100 (MQ=255)
                                   cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa                                                             >  2:163224/1‑100 (MQ=255)
                                   cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaa                                                             <  2:808217/100‑1 (MQ=255)
                                   cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaa                                                            >  2:3288408/1‑100 (MQ=255)
                                     gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                           >  2:114300/1‑100 (MQ=255)
                                     gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                           >  2:903115/1‑100 (MQ=255)
                                     gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                           >  2:483696/1‑100 (MQ=255)
                                     gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                           >  1:110451/1‑100 (MQ=255)
                                      cAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                          >  2:1681741/1‑100 (MQ=255)
                                       aaaGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAAt                                                         >  2:2450341/1‑100 (MQ=255)
                                         aGAAACTTAAATATAAAAAATATCTATTTTTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAg                                                       <  1:2846811/100‑1 (MQ=255)
                                         aGAAACTGCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGAGTATTAGGAAGGGAATTAATGCAATTAAAAAAAAAAAAAAAAATATCCAAAAATAg                                                       <  1:1047286/100‑1 (MQ=255)
                                          gAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGt                                                      <  1:3153077/100‑1 (MQ=255)
                                           aaCCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGta                                                     <  1:1356575/100‑1 (MQ=255)
                                             ccTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtata                                                   >  2:1815201/1‑100 (MQ=255)
                                               ttCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACt                                                 >  2:2088491/1‑100 (MQ=255)
                                               ttCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACt                                                 >  2:3417199/1‑100 (MQ=255)
                                                 cAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACt                                                 <  1:2227993/98‑1 (MQ=255)
                                                 cAACATGAAAAATATCCATTTGTCCGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACt                                                 <  2:2891997/98‑1 (MQ=255)
                                                  aaCATGAAAAATATCCATTTGTTTGTAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCTAAAAATAGTATACtt                                                <  1:1377104/98‑1 (MQ=255)
                                                     aTGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTAt                                             <  1:1739506/98‑1 (MQ=255)
                                                      tGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                          >  2:2562700/1‑100 (MQ=255)
                                                       gAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa                                         >  1:1256435/1‑100 (MQ=255)
                                                       gAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa                                         >  1:455673/1‑100 (MQ=255)
                                                        aaaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAACTTCAAGAAATATCCGAAAATAGTATACTTTATTCCag                                        >  1:2339367/1‑100 (MQ=255)
                                                        aaaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                          <  1:328025/98‑1 (MQ=255)
                                                        aaaaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag                                        >  1:1464564/1‑100 (MQ=255)
                                                          aaaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCaga                                       <  1:163222/99‑1 (MQ=255)
                                                           aaTATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaag                                     <  1:3429867/100‑1 (MQ=255)
                                                           aaTATCCATTTGTTTGAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaag                                     <  1:836181/100‑1 (MQ=255)
                                                            atatCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaaga                                    >  2:1893475/1‑100 (MQ=255)
                                                              atCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGt                                  >  2:22523/1‑100 (MQ=255)
                                                                ccATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTc                                >  2:3114802/1‑100 (MQ=255)
                                                                 cATTTGTTTGCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCa                               <  2:3126252/100‑1 (MQ=255)
                                                                       tttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATaa                         >  1:505014/1‑100 (MQ=255)
                                                                        ttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAAt                        <  2:244698/100‑1 (MQ=255)
                                                                        ttgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAAt                        >  1:2555238/1‑100 (MQ=255)
                                                                         tgCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATg                       <  2:1540076/100‑1 (MQ=255)
                                                                          gCAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                      <  2:138664/100‑1 (MQ=255)
                                                                           cAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGtt                     <  1:3431683/100‑1 (MQ=255)
                                                                           cAAAAAAAGATTATTAGGAAGGGAATTAATGCAAATATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAAt                        >  1:1153843/1‑97 (MQ=255)
                                                                            aaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGttt                    <  1:1441087/100‑1 (MQ=255)
                                                                            aaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGttt                    >  1:2639531/1‑100 (MQ=255)
                                                                            aaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGttt                    >  1:2754598/1‑100 (MQ=255)
                                                                            aaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGttt                    >  2:2048699/1‑100 (MQ=255)
                                                                             aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                   <  1:2875561/100‑1 (MQ=255)
                                                                             aaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                   >  2:661589/1‑100 (MQ=255)
                                                                             aaaaaaGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                   <  2:2812841/100‑1 (MQ=255)
                                                                                aaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCt                <  2:420025/100‑1 (MQ=255)
                                                                                aaaGATTATTAGGAAGGGAATTAATGCAATTATAGAAAATTGGAAAAATATTAAAAAATAGTATACATAATAACAGAAGAGTTCAAaataatatattttt                >  1:3343295/1‑92 (MQ=255)
                                                                                 aaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCtt               >  2:3086896/1‑100 (MQ=255)
                                                                                 aaGATTATAAGGAAGGGAATTAATGCAATTATCGAAAATGCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCtt               <  1:630111/100‑1 (MQ=255)
                                                                                   gATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCa             <  2:149039/100‑1 (MQ=255)
                                                                                      tattaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtt          <  2:618052/100‑1 (MQ=255)
                                                                                      tattaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtt          >  1:1976233/1‑100 (MQ=255)
                                                                                        ttaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtttt        <  1:1676762/100‑1 (MQ=255)
                                                                                        ttaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtttt        <  1:2048668/100‑1 (MQ=255)
                                                                                          aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  1:1012502/100‑1 (MQ=255)
                                                                                          aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  2:29664/100‑1 (MQ=255)
                                                                                          aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  2:1367447/100‑1 (MQ=255)
                                                                                          aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  2:1159003/100‑1 (MQ=255)
                                                                                          aggaagggAATTAATGCAATTATCGAAAATTCAAAAAATATACAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  1:1815176/100‑1 (MQ=255)
                                                                                          aggaaggaAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc      <  2:476674/100‑1 (MQ=255)
                                                                                           ggaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCt     >  1:1326734/1‑100 (MQ=255)
                                                                                            gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    >  2:3062941/1‑100 (MQ=255)
                                                                                            gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    <  2:3286730/100‑1 (MQ=255)
                                                                                            gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    <  2:2004053/100‑1 (MQ=255)
                                                                                            gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    <  1:643272/100‑1 (MQ=255)
                                                                                            gaagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    <  1:2977100/100‑1 (MQ=255)
                                                                                            gaagggaATTAATGCAATTATCGAAAATTCAAAAAATAGCCAAAAATAGTATACTTTACTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCtt    <  1:1897320/100‑1 (MQ=255)
                                                                                             aagggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTa   >  1:1832549/1‑100 (MQ=255)
                                                                                              agggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTa   >  1:1949179/1‑99 (MQ=255)
                                                                                              agggaATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTAc  >  2:1910936/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTAC  >  NC_000913/1197642‑1197835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: