Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,292,944 T→C intergenic (‑22/‑583) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,9440TC98.5% 463.9 / ‑3.0 136intergenic (‑22/‑583)hns/tdkglobal DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (72/61);  total (73/63)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 136 total aligned reads displayed.

GCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCG  >  NC_000913/1292847‑1293039
                                                                                                 |                                                                                               
   tttCAAGTGTACATTCTCTTGCCTGCGCACGACGAGTACAGAAGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgggg                                                                                            <  1:2436159/100‑1 (MQ=255)
    ttCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggt                                                                                           >  2:1114500/1‑100 (MQ=255)
    ttCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggt                                                                                           >  1:2930578/1‑100 (MQ=255)
      cAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtgg                                                                                         >  2:964117/1‑100 (MQ=255)
      cAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtgg                                                                                         >  1:2478578/1‑100 (MQ=255)
        aGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGtt                                                                                       <  2:1928351/100‑1 (MQ=255)
        aGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGtt                                                                                       <  2:1102700/100‑1 (MQ=255)
        aGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGtt                                                                                       <  1:1731603/100‑1 (MQ=255)
        aGTGTACATTCTCTTGCCTGCGCACGAAGAATACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATAATGGGGTGGtt                                                                                       >  1:2284598/1‑100 (MQ=255)
          tgtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttg                                                                                     >  2:1522565/1‑100 (MQ=255)
          tgtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttg                                                                                     <  2:1407178/100‑1 (MQ=255)
           gtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgt                                                                                    >  2:3101466/1‑100 (MQ=255)
           gtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgt                                                                                    >  1:1935580/1‑100 (MQ=255)
              cATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGa                                                                                 <  1:721449/100‑1 (MQ=255)
               aTTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAg                                                                                <  1:3416074/100‑1 (MQ=255)
               aTTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAg                                                                                >  1:1571759/1‑100 (MQ=255)
                ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                               <  2:702722/100‑1 (MQ=255)
                ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                               <  2:1889065/100‑1 (MQ=255)
                ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                               <  2:2689885/100‑1 (MQ=255)
                 tctcTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGt                                                                              <  1:2719953/100‑1 (MQ=255)
                 tctcTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGt                                                                              >  2:2996418/1‑100 (MQ=255)
                  ctctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGta                                                                             >  2:2519198/1‑100 (MQ=255)
                   tctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaa                                                                            >  1:65099/1‑100 (MQ=255)
                    ctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaat                                                                           >  2:3470638/1‑100 (MQ=255)
                    ctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaat                                                                           <  1:462508/100‑1 (MQ=255)
                    ctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaat                                                                           <  2:2429163/100‑1 (MQ=255)
                        ccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAataata                                                                       <  1:90569/100‑1 (MQ=255)
                          tGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATaga                                                                     <  2:2708765/100‑1 (MQ=255)
                           gcgcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATagag                                                                    <  1:224063/100‑1 (MQ=255)
                            cgcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGc                                                                   <  1:186597/100‑1 (MQ=255)
                            cgcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGATTGTTGAGGTAATAATAGAGc                                                                   >  1:2874902/1‑100 (MQ=255)
                              cACGAGGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCt                                                                 >  2:403469/1‑100 (MQ=255)
                                cGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTa                                                               >  1:2814296/1‑100 (MQ=255)
                                  aaGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTaaa                                                             <  2:1211998/100‑1 (MQ=255)
                                   agagTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAAt                                                            <  2:38310/100‑1 (MQ=255)
                                    gagTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAAtt                                                           >  1:3290346/1‑100 (MQ=255)
                                         cGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGtt                                                      <  1:1511277/100‑1 (MQ=255)
                                          ggATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtg                                                     <  2:454249/100‑1 (MQ=255)
                                          ggATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtg                                                     <  2:3191270/100‑1 (MQ=255)
                                          ggAGGTTGTTCATAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtg                                                     <  2:1506371/100‑1 (MQ=255)
                                             tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                  <  1:209904/100‑1 (MQ=255)
                                             tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                  <  1:3086690/100‑1 (MQ=255)
                                             tgttgtTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                                  <  1:3101416/100‑1 (MQ=255)
                                              cttgttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCa                                                 <  2:2143530/99‑1 (MQ=255)
                                                tgttCAGAATTTTTAGTGCTTCGCTCATTGTAGTAATCACAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCaag                                               >  1:2143499/1‑99 (MQ=255)
                                                tgttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAAt                                               <  1:2980550/100‑1 (MQ=255)
                                                 gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAATTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGGCTTAAATTTAGTTGTGCAATa                                              >  1:630847/1‑100 (MQ=255)
                                                 gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATa                                              <  1:2128824/100‑1 (MQ=255)
                                                 gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTATGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAAt                                               >  1:1069536/1‑99 (MQ=255)
                                                  ttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAg                                             >  1:1497728/1‑100 (MQ=255)
                                                    cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcn                                           <  1:2804252/100‑2 (MQ=255)
                                                    cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                           >  1:1592071/1‑100 (MQ=255)
                                                    cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                           <  1:1291831/100‑1 (MQ=255)
                                                    cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                           >  2:1677180/1‑100 (MQ=255)
                                                    cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                           >  1:490201/1‑100 (MQ=255)
                                                     aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCANTAGCCa                                          >  2:2823911/1‑100 (MQ=255)
                                                     aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                          >  2:3451540/1‑100 (MQ=255)
                                                     aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                          >  2:2541997/1‑100 (MQ=255)
                                                      gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                         >  2:3436713/1‑100 (MQ=255)
                                                      gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                         >  1:1920701/1‑100 (MQ=255)
                                                      gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                         >  2:1698374/1‑100 (MQ=255)
                                                       aaTTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAAGTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAgg                                        >  2:2451946/1‑100 (MQ=255)
                                                       aaTTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAgg                                        >  2:540067/1‑100 (MQ=255)
                                                        aTTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGa                                       >  1:735722/1‑100 (MQ=255)
                                                          tttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGaa                                      >  1:1043396/1‑99 (MQ=255)
                                                          tttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAAt                                     >  1:1177535/1‑100 (MQ=255)
                                                            tAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGt                                   >  2:2955921/1‑100 (MQ=255)
                                                            tAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGt                                   <  2:2790173/100‑1 (MQ=255)
                                                               gTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAg                                <  1:1231640/100‑1 (MQ=255)
                                                                 gCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGa                              >  1:1147334/1‑100 (MQ=255)
                                                                 gCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGa                              >  2:3131183/1‑100 (MQ=255)
                                                                   ttCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAAt                            <  2:693759/100‑1 (MQ=255)
                                                                    tCGCTCATTGTAGTAATCTCAAACTTATACTGCGGTGGTTTGTTGAGGTAATAATAGAGCCTTATATCCAGTTGTGCAATAGCCAGGCATGTAAGGAAtt                           >  1:738030/1‑100 (MQ=255)
                                                                     cGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTc                          >  1:1955409/1‑100 (MQ=255)
                                                                     cGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTc                          >  1:569970/1‑100 (MQ=255)
                                                                      gCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCa                         >  2:469042/1‑100 (MQ=255)
                                                                      gCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCa                         >  2:651600/1‑100 (MQ=255)
                                                                      gCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCa                         >  1:2686212/1‑100 (MQ=255)
                                                                          cgatctgtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAAt                     <  2:1356072/94‑1 (MQ=255)
                                                                          aTTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATCATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAAt                     >  2:3101791/1‑100 (MQ=255)
                                                                          aTTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAAt                     >  1:1647983/1‑100 (MQ=255)
                                                                            tGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTg                   <  1:1361073/100‑1 (MQ=255)
                                                                            tGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTg                   >  2:2225729/1‑100 (MQ=255)
                                                                            tGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTg                   <  1:485906/100‑1 (MQ=255)
                                                                             gtagtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGt                  >  1:2636923/1‑100 (MQ=255)
                                                                             gtagtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGt                  >  2:2903668/1‑100 (MQ=255)
                                                                              tagtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGtt                 >  1:555710/1‑100 (MQ=255)
                                                                                gtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAAtagatcg               >  1:1356053/1‑94 (MQ=255)
                                                                                gtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCt               >  2:3175751/1‑100 (MQ=255)
                                                                                gtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCt               >  2:195169/1‑100 (MQ=255)
                                                                                 taATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCtn              <  2:2561578/100‑2 (MQ=255)
                                                                                 taATCACAATCGTATTCTGGGGTGGTTTGTTTTGGTATTAATAGAGTCTTAAATTCAGTTGTGCAATAGCTAGGAATGTAAGGTATTTAATATTGTTCtt              <  1:2256786/100‑1 (MQ=255)
                                                                                     ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt          >  1:681520/1‑100 (MQ=255)
                                                                                     ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt          >  1:1335980/1‑100 (MQ=255)
                                                                                     ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt          >  1:1088773/1‑100 (MQ=255)
                                                                                      tcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt         >  2:1029521/1‑100 (MQ=255)
                                                                                       cAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtttt        >  2:772712/1‑100 (MQ=255)
                                                                                           cTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTgc    >  2:2151304/1‑100 (MQ=255)
                                                                                            ttATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTgcc   <  1:2955873/100‑1 (MQ=255)
                                                                                             tataCTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTgccg  <  1:3470582/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCG  >  NC_000913/1292847‑1293039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: