BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,634	T→A	I338F (ATC→TTC)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,634	0	T	A	97.0%	447.3 / 2.5	135	I338F (ATC→TTC)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base T (3/1); new base A (67/64); total (70/65)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 135 total aligned reads displayed.

CGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGAC  >  NC_000913/1307539‑1307732

cgGAAGGATAATACTGACATCCTCCCCGCGCCGCGCCTCCGTGCAAAGCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACaaa                                                                                                <  1:2346190/100‑1 (MQ=255)

cgGAAGGATAATACTGACATCCACCCCGCGCTGCGCTGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACaaa                                                                                                >  1:242577/1‑100 (MQ=255)

cgGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACaaa                                                                                                >  2:2149345/1‑100 (MQ=255)

 ggAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAat                                                                                               >  2:438760/1‑100 (MQ=255)

    aGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATAtt                                                                                            <  1:3002103/100‑1 (MQ=255)

     ggATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATAATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTc                                                                                           >  1:2444510/1‑100 (MQ=255)

      gATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCa                                                                                          >  2:859986/1‑100 (MQ=255)

      gATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCa                                                                                          >  2:571099/1‑100 (MQ=255)

        taataCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCAcg                                                                                        <  1:31685/100‑1 (MQ=255)

          ataCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCAcgcg                                                                                      <  2:1171866/100‑1 (MQ=255)

          ataCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCAcgcg                                                                                      >  2:2208600/1‑100 (MQ=255)

            aCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACgcgc                                                                                     >  1:380611/1‑99 (MQ=255)

            aCTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGcc                                                                                    <  1:2196884/100‑1 (MQ=255)

             cTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCg                                                                                   >  1:3370304/1‑100 (MQ=255)

             cTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCg                                                                                   <  2:974830/100‑1 (MQ=255)

               gACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGaa                                                                                 >  1:2659600/1‑100 (MQ=255)

                 cATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATa                                                                               >  1:950784/1‑100 (MQ=255)

                  aTCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATaa                                                                              >  1:775658/1‑100 (MQ=255)

                  aTCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATaa                                                                              <  1:2198819/100‑1 (MQ=255)

                   tCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAGATATTCACGCGCCGAATAAg                                                                             <  1:2125858/100‑1 (MQ=255)

                   tCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAg                                                                             >  1:593212/1‑100 (MQ=255)

                   tCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAg                                                                             >  2:354462/1‑100 (MQ=255)

                    ccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGc                                                                            <  2:3103474/100‑1 (MQ=255)

                    ccACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGc                                                                            <  2:3446539/100‑1 (MQ=255)

                        cccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCa                                                                        >  2:30357/1‑100 (MQ=255)

                         ccGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAg                                                                       >  2:2207976/1‑100 (MQ=255)

                           gcgcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTc                                                                     <  2:631015/100‑1 (MQ=255)

                           gcgcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTc                                                                     <  2:310624/100‑1 (MQ=255)

                            cgcTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCa                                                                    <  1:2508153/100‑1 (MQ=255)

                               tGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCaatc                                                                 <  2:3178481/100‑2 (MQ=255)

                                gcgcCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCaataa                                                                >  2:2741395/1‑100 (MQ=255)

                                  gccgccGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAACGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATg                                                              >  1:2889317/1‑100 (MQ=255)

                                   ccgccgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGc                                                             <  2:3045828/100‑1 (MQ=255)

                                      ccgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTg                                                          <  2:1610870/100‑1 (MQ=255)

                                       cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                         <  1:3257845/100‑1 (MQ=255)

                                       cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                         <  2:463790/100‑1 (MQ=255)

                                       cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                         <  1:619870/100‑1 (MQ=255)

                                       cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                         <  2:1182547/100‑1 (MQ=255)

                                       cgTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTgg                                                         <  2:131841/100‑1 (MQ=255)

                                        gTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGt                                                        >  1:845222/1‑100 (MQ=255)

                                         tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                       >  2:1082247/1‑100 (MQ=255)

                                         tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                       >  1:2245491/1‑100 (MQ=255)

                                         tGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTg                                                       <  2:2473206/100‑1 (MQ=255)

                                          gCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGa                                                      >  1:2397247/1‑100 (MQ=255)

                                           cAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGaa                                                     <  2:2116994/100‑1 (MQ=255)

                                             aaTCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATg                                                   >  1:1263291/1‑100 (MQ=255)

                                             aaTCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATg                                                   >  1:1739858/1‑100 (MQ=255)

                                             aaTCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATg                                                   <  2:1089225/100‑1 (MQ=255)

                                              aTCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCATGGTGAATga                                                  >  1:2279704/1‑100 (MQ=255)

                                                cGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATgaga                                                <  2:2649019/100‑1 (MQ=255)

                                                cGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATgaga                                                <  2:815546/100‑1 (MQ=255)

                                                cGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAAAATTCACGCGCCGAATACGCCGCAGTCAATAATGCCAgg                                                         >  1:2782712/1‑91 (MQ=255)

                                                 gCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGAt                                               >  2:2823684/1‑100 (MQ=255)

                                                 gCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAACGCCTGGTGAATgag                                                 >  1:2030252/1‑98 (MQ=255)

                                                   aTGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCt                                             >  1:684763/1‑100 (MQ=255)

                                                   aTGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCt                                             >  1:684929/1‑100 (MQ=255)

                                                      aaGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTcc                                          <  2:508993/100‑1 (MQ=255)

                                                       aGTAAATCATCGCTTGGCACAAAGTAGGTCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCg                                         <  1:2257228/100‑1 (MQ=255)

                                                        gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                        <  1:2309901/100‑1 (MQ=255)

                                                        gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                        >  2:2049505/1‑100 (MQ=255)

                                                        gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                        <  1:2500546/100‑1 (MQ=255)

                                                        gTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCgg                                        <  1:1705720/100‑1 (MQ=255)

                                                          naaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGaa                                      >  1:539965/2‑100 (MQ=255)

                                                          aaaTCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGaa                                      >  2:3065703/1‑100 (MQ=255)

                                                          aaaTCATCGCTTGGCACAAAGGAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGaa                                      <  1:1390630/100‑1 (MQ=255)

                                                              catcGCTTGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGcc                                  >  2:2878811/1‑100 (MQ=255)

                                                                 cGCTTGGCACAAAGTAGGGCGTGGTCATGAATAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCggg                               <  2:1741257/100‑1 (MQ=255)

                                                                     tGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCa                           <  2:154140/100‑1 (MQ=255)

                                                                     tGGCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCa                           <  1:191488/100‑1 (MQ=255)

                                                                       gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCACTAATGCCTGATGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                         >  1:2441667/1‑100 (MQ=255)

                                                                       gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                         <  2:2659638/100‑1 (MQ=255)

                                                                       gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                         <  2:979647/100‑1 (MQ=255)

                                                                       gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                         <  1:593368/100‑1 (MQ=255)

                                                                       gCACAAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCATGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGa                         >  1:2004965/1‑100 (MQ=255)

                                                                        cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATACGCCGCAGTCAATAATGCCTGGTGAATGAAATCTTCCGGAAAGCCGGGGCCAGaa                        >  1:778799/1‑100 (MQ=255)

                                                                        cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                        >  1:1235024/1‑100 (MQ=255)

                                                                        cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                        >  2:3449976/1‑100 (MQ=255)

                                                                        cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGaa                        >  1:450310/1‑100 (MQ=255)

                                                                        cacaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGAAAAGCCGGGGCCAGaa                        >  1:2033922/1‑100 (MQ=255)

                                                                         acaAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAg                       >  2:2193753/1‑100 (MQ=255)

                                                                         acaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAg                       >  2:1441288/1‑100 (MQ=255)

                                                                         acaAAGTAGGGCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAg                       <  1:2248293/100‑1 (MQ=255)

                                                                          caAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGc                      >  2:891852/1‑100 (MQ=255)

                                                                                 gggCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtg               <  1:2955673/100‑1 (MQ=255)

                                                                                 gggCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtg               <  2:702517/100‑1 (MQ=255)

                                                                                 gggCGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtg               >  1:1953894/1‑100 (MQ=255)

                                                                                    cGTGGTCATGAACAAATATTCACGCGTCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGa            <  1:1480345/100‑1 (MQ=255)

                                                                                    cGTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATtgtgtt             <  1:143818/99‑2 (MQ=255)

                                                                                     gTGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGaa           <  1:204024/100‑1 (MQ=255)

                                                                                      tGGTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAAt          >  1:3159514/1‑100 (MQ=255)

                                                                                       tcttccgATCTAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATg         <  1:3453619/94‑1 (MQ=255)

                                                                                       ggTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATg         >  1:2632510/1‑100 (MQ=255)

                                                                                       ggTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATg         <  1:1220163/100‑1 (MQ=255)

                                                                                        gTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATgg        >  1:2769164/1‑100 (MQ=255)

                                                                                        gTCATGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATgg        >  2:2600216/1‑100 (MQ=255)

                                                                                           aTGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgtgt     <  1:3307627/100‑1 (MQ=255)

                                                                                           aTGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgtg      <  1:1366545/99‑1 (MQ=255)

                                                                                            tGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGtgtg    >  2:2274694/1‑100 (MQ=255)

                                                                                            tGAACAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCAGGGGCCAGAAGCAATTGTGTGAATGGtgtg    >  1:2370923/1‑100 (MQ=255)

                                                                                              aTCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGAc  >  2:2491646/1‑100 (MQ=255)

CGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGAC  >  NC_000913/1307539‑1307732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`