Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,435,737 G→A intergenic (‑118/+23) uspF ← / ← ompN stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,435,7370GA94.4% 370.9 / 7.0 127intergenic (‑118/+23)uspF/ompNstress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base A (56/63);  total (59/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 129 total aligned reads displayed.

CCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCA  >  NC_000913/1435640‑1435832
                                                                                                 |                                                                                               
ccGCATAAAGTGTAACGCCAGATGACACTTTTTATGTAATGACGGAGTTCACATTTTTAATTTAGATCAATGGAGGAAGAATAAGCAGAAAAAGCCCAcc                                                                                               >  1:2096080/1‑100 (MQ=255)
 cGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCccacca                                                                                              <  2:3292110/100‑1 (MQ=255)
     tAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAAc                                                                                          >  1:3042894/1‑100 (MQ=255)
     tAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAAc                                                                                          >  1:1711213/1‑100 (MQ=255)
           gtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCggg                                                                                    >  1:793917/1‑100 (MQ=255)
           gtAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCggg                                                                                    <  2:1274135/100‑1 (MQ=255)
             aaCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCa                                                                                  >  2:520433/1‑100 (MQ=255)
             aaCGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCa                                                                                  >  1:3226345/1‑100 (MQ=255)
                 ccAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCaggag                                                                              <  1:3438091/100‑1 (MQ=255)
                  cAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCaggagg                                                                             <  2:3224684/100‑1 (MQ=255)
                     aTGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGAtt                                                                          <  2:579708/100‑1 (MQ=255)
                      tGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGAttt                                                                         <  1:2197895/100‑1 (MQ=255)
                        acacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAg                                                                       <  2:3152074/100‑1 (MQ=255)
                         cacTTTTTGTTTTTTTATGTAGTTCACATTTTTAATTTAGATTAAAGGTGGTAGAATAAGCAGAAAAAGCTTACCATCACAGCGGTAAGGATGATTTat                                                                       >  1:1587744/1‑98 (MQ=255)
                         cacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGa                                                                      >  2:3159950/1‑100 (MQ=255)
                         cacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGa                                                                      >  2:1217242/1‑100 (MQ=255)
                         cacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGa                                                                      <  1:1996301/100‑1 (MQ=255)
                         cacTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGa                                                                      <  1:3083760/100‑1 (MQ=255)
                           cTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAAc                                                                    <  1:445386/100‑1 (MQ=255)
                            tttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACt                                                                   >  1:432757/1‑100 (MQ=255)
                             ttttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTg                                                                  <  1:1540087/100‑1 (MQ=255)
                              tttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTg                                                                  <  1:2805913/99‑1 (MQ=255)
                              tttGTGTAATGACGGAGTTCACATTTTTAATGTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGa                                                                 <  2:2615162/100‑1 (MQ=255)
                               ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                <  1:3449925/100‑1 (MQ=255)
                               ttGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAAAAAAAGAAAAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGAt                                                                <  1:1932595/100‑1 (MQ=255)
                                  tgtAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATaaa                                                             >  2:2275024/1‑100 (MQ=255)
                                   gtAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc                                                            >  1:1536884/1‑100 (MQ=255)
                                   gtAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAc                                                            >  2:1996492/1‑100 (MQ=255)
                                    tAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAcc                                                           >  2:1054325/1‑100 (MQ=255)
                                    tAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAAcc                                                           <  2:1225713/100‑1 (MQ=255)
                                        gACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc                                                       >  1:796243/1‑100 (MQ=255)
                                        gACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAc                                                       >  2:3382068/1‑100 (MQ=255)
                                         aCGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGAcc                                                      <  1:3159900/100‑1 (MQ=255)
                                          cGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCt                                                     >  1:507327/1‑100 (MQ=255)
                                          cGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCt                                                     >  1:3227687/1‑100 (MQ=255)
                                           ggAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTa                                                    >  1:492402/1‑100 (MQ=255)
                                           ggAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGACGAAAAAGCCCGCCATAACAGCGAGCAGGAGGATTTAGAGCGGATAAAc                                                            >  1:2766499/1‑92 (MQ=255)
                                              gTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAg                                                 <  1:1664150/100‑1 (MQ=255)
                                               ttCACATTTTTGATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                >  2:108004/1‑100 (MQ=255)
                                               ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                <  1:2160079/100‑1 (MQ=255)
                                               ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                >  2:2537702/1‑100 (MQ=255)
                                               ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                <  2:807647/100‑1 (MQ=255)
                                               ttCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGc                                                >  1:2425533/1‑100 (MQ=255)
                                                 cacaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGa                                              >  1:2644100/1‑100 (MQ=255)
                                                 cacaTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGa                                              >  1:921665/1‑100 (MQ=255)
                                                   caTTTTTAATTTATATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                            >  1:309052/1‑100 (MQ=255)
                                                   caTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                            >  1:787478/1‑100 (MQ=255)
                                                    aTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACaa                                           >  1:1663361/1‑100 (MQ=255)
                                                    aTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACaa                                           <  2:1344457/100‑1 (MQ=255)
                                                    aTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACaa                                           <  1:1720233/100‑1 (MQ=255)
                                                    aTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACaa                                           >  2:2387613/1‑100 (MQ=255)
                                                    aTTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACa                                            <  1:183828/99‑1 (MQ=255)
                                                      ttttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAata                                         >  1:412308/1‑100 (MQ=255)
                                                      ttttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAata                                         <  2:522050/100‑1 (MQ=255)
                                                       tttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAatat                                        <  2:3151821/100‑1 (MQ=255)
                                                       tttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAatat                                        <  2:3471745/100‑1 (MQ=255)
                                                        ttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatc                                       <  1:558925/100‑1 (MQ=255)
                                                         tAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatca                                      <  2:1013999/100‑1 (MQ=255)
                                                           aTTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATatcatc                                    >  2:2930104/1‑100 (MQ=255)
                                                              tAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGt                                 <  1:1094523/100‑1 (MQ=255)
                                                              tAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                <  1:3257594/100‑1 (MQ=255)
                                                                gATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTa                                >  1:1818119/1‑99 (MQ=255)
                                                                gATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTag                               <  1:765969/100‑1 (MQ=255)
                                                                gATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTag                               >  2:236301/1‑100 (MQ=255)
                                                                 aTCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTaga                              <  1:1996463/100‑1 (MQ=255)
                                                                 aTCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTaga                              <  1:3004265/100‑1 (MQ=255)
                                                                  tCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTagag                             >  2:1055650/1‑100 (MQ=255)
                                                                  tCAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTaga                              >  1:2260088/1‑99 (MQ=255)
                                                                   cAAAGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAgaga                            >  1:3424844/1‑100 (MQ=255)
                                                                     aaGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGTAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATg                          >  2:2521125/1‑100 (MQ=255)
                                                                     aaGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATg                          <  2:3165580/100‑1 (MQ=255)
                                                                     aaGGAGGAAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATg                          <  1:1879642/100‑1 (MQ=255)
                                                                       ggaggaAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGcc                        >  1:2773276/1‑100 (MQ=255)
                                                                       ggaggaAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGACTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGAAGAGATGcc                        >  1:194601/1‑100 (MQ=255)
                                                                         aggaAGAATAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCAt                      <  2:3212889/100‑1 (MQ=255)
                                                                            aagaaTAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGACTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTgg                   <  1:1369709/100‑1 (MQ=255)
                                                                            aagaaTAAGCAGAAAAAGCCCGCCACAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTgg                   <  2:3207961/100‑1 (MQ=255)
                                                                               aaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg                <  1:1188348/100‑1 (MQ=255)
                                                                               aaTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAg                <  1:2877549/100‑1 (MQ=255)
                                                                                aTAAGCAGAAAAAGCCCACCCTAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGc               >  1:2125849/1‑100 (MQ=255)
                                                                                aTAAGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGc               >  2:1539379/1‑100 (MQ=255)
                                                                                  aaGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGt             <  2:514060/100‑1 (MQ=255)
                                                                                   aGCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTa            >  1:2294368/1‑100 (MQ=255)
                                                                                    gCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg           >  2:1140673/1‑100 (MQ=255)
                                                                                    gCAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAg           >  2:1618671/1‑100 (MQ=255)
                                                                                     cAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa          >  2:389294/1‑100 (MQ=255)
                                                                                     cAGAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGa          <  2:2096111/100‑1 (MQ=255)
                                                                                       gAAAAAGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAg        <  2:1816679/100‑1 (MQ=255)
                                                                                        aaaaaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGc       <  1:230198/100‑1 (MQ=255)
                                                                                        aaaaaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGc       <  1:2553021/100‑1 (MQ=255)
                                                                                         aaaaGCCCACCATAACAGCGGGCCGGAGGCTTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGc       <  2:1896240/99‑1 (MQ=255)
                                                                                          aaaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTg     <  2:2792674/100‑1 (MQ=255)
                                                                                          aaaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTg     <  1:966430/100‑1 (MQ=255)
                                                                                          aaaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTg     >  1:471528/1‑100 (MQ=255)
                                                                                           aaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGt    <  2:1699786/100‑1 (MQ=255)
                                                                                           aaGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGt    <  2:1698578/100‑1 (MQ=255)
                                                                                            aGCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTc   >  1:2643601/1‑100 (MQ=255)
                                                                                             gCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCa  <  1:108003/100‑1 (MQ=255)
                                                                                             gCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCa  <  1:2494939/100‑1 (MQ=255)
                                                                                             gCCCACCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCa  <  2:793928/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGATGCCATTGGCAGCGTAGAAGCTGTCA  >  NC_000913/1435640‑1435832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: