Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,493,774 A→G L435L (TTA→TTG)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,493,7740AG98.5% 460.3 / ‑3.3 135L435L (TTA→TTG) trgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (64/68);  total (65/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 135 total aligned reads displayed.

GATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAT  >  NC_000913/1493677‑1493869
                                                                                                 |                                                                                               
gATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGAt                                                                                               <  2:1634545/100‑1 (MQ=255)
  tttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTg                                                                                             <  1:447068/100‑1 (MQ=255)
   ttGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTg                                                                                             <  1:2316661/99‑1 (MQ=255)
    tGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGAc                                                                                           <  1:494718/100‑1 (MQ=255)
     gCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGAcc                                                                                          <  2:171980/100‑1 (MQ=255)
     gCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGAcc                                                                                          <  1:1792810/100‑1 (MQ=255)
      ccGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCt                                                                                         >  2:2238540/1‑100 (MQ=255)
       cGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTg                                                                                        >  1:3379529/1‑100 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgg                                                                                       <  1:180770/100‑1 (MQ=255)
        gTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgg                                                                                       >  1:2304748/1‑100 (MQ=255)
          tGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgggg                                                                                     <  2:2937860/100‑1 (MQ=255)
          tGTCACCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTgggg                                                                                     >  2:1326396/1‑100 (MQ=255)
           gTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGt                                                                                    >  2:2203055/1‑100 (MQ=255)
             cGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCg                                                                                  >  1:477762/1‑100 (MQ=255)
              gCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCgg                                                                                 >  2:256606/1‑100 (MQ=255)
                   cGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAg                                                                            >  2:2617152/1‑100 (MQ=255)
                   cGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAg                                                                            >  2:1720169/1‑100 (MQ=255)
                    gAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCGGTCAGGTTGATTGACCTGGGGTCGGATGAgg                                                                           <  2:3054995/100‑1 (MQ=255)
                     aaGTACGGACACTCGCAAGTCGTAGCGCTCATGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGt                                                                          <  1:2339497/100‑1 (MQ=255)
                     aaGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGt                                                                          <  1:2692824/100‑1 (MQ=255)
                     aaGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGt                                                                          <  2:522931/100‑1 (MQ=255)
                      aGTACGGACACTCGCAAGTCGCAGCGCTAAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTg                                                                         <  1:1556828/100‑1 (MQ=255)
                       gTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTgg                                                                        >  2:3184297/1‑100 (MQ=255)
                       gTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTgg                                                                        <  1:1081775/100‑1 (MQ=255)
                       gTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTgg                                                                        <  1:2828964/100‑1 (MQ=255)
                         aCGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCa                                                                      >  1:199141/1‑100 (MQ=255)
                            gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACg                                                                   >  2:3029798/1‑100 (MQ=255)
                            gACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACg                                                                   >  2:884064/1‑100 (MQ=255)
                               acTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGcc                                                                >  1:2780594/1‑100 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                               >  1:1245721/1‑100 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                               >  2:177036/1‑100 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                               >  2:270842/1‑100 (MQ=255)
                                cTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                               >  1:365489/1‑100 (MQ=255)
                                 tCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCgg                                                              >  1:2977205/1‑100 (MQ=255)
                                 tCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCgg                                                              <  2:1046013/100‑1 (MQ=255)
                                  cGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCg                                                               <  1:1869777/98‑1 (MQ=255)
                                    cAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGaa                                                           >  2:1327543/1‑100 (MQ=255)
                                       gTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAAc                                                        >  2:1018786/1‑100 (MQ=255)
                                       gTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAAc                                                        >  2:728244/1‑100 (MQ=255)
                                       gTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAAc                                                        <  2:1259991/100‑1 (MQ=255)
                                         cGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCa                                                      >  2:2825950/1‑100 (MQ=255)
                                          gCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAt                                                     >  1:908493/1‑100 (MQ=255)
                                          gCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAt                                                     <  2:3051223/100‑1 (MQ=255)
                                          gCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAt                                                     >  2:2109139/1‑100 (MQ=255)
                                          gCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCAt                                                     >  1:17240/1‑100 (MQ=255)
                                           cAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATg                                                    <  2:2328898/100‑1 (MQ=255)
                                           cAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATg                                                    <  2:2537612/100‑1 (MQ=255)
                                           cAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATg                                                    <  1:2492475/100‑1 (MQ=255)
                                           cAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATg                                                    >  1:2045178/1‑100 (MQ=255)
                                             gcgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAg                                                  <  1:2246693/100‑1 (MQ=255)
                                              cgcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGc                                                 >  1:1524643/1‑100 (MQ=255)
                                               gcTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCa                                                <  2:469540/100‑1 (MQ=255)
                                                cTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCAc                                               <  1:160087/100‑1 (MQ=255)
                                                cTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCAc                                               <  2:1248532/100‑1 (MQ=255)
                                                  cAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTa                                             >  2:1863283/1‑100 (MQ=255)
                                                  cAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTa                                             >  1:866735/1‑100 (MQ=255)
                                                    ggcggcGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAtt                                           >  2:882340/1‑100 (MQ=255)
                                                       ggcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgtt                                        <  2:1657504/100‑1 (MQ=255)
                                                       ggcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGCGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgtt                                        <  1:1934067/100‑1 (MQ=255)
                                                        gggAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                       <  1:752814/98‑1 (MQ=255)
                                                        gcgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTAttgttg                                       <  2:2004781/100‑1 (MQ=255)
                                                         cgAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGa                                      >  2:2438139/1‑100 (MQ=255)
                                                          gAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACAATTGTTGAt                                     >  2:2162723/1‑100 (MQ=255)
                                                            aaGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGc                                   >  1:1665059/1‑100 (MQ=255)
                                                             agagATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGcc                                  <  1:2094993/100‑1 (MQ=255)
                                                             agagATTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGAAAAAAAAAACCATGAGCACTATTGTTGATGcc                                  <  1:144666/100‑1 (MQ=255)
                                                              gagaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGcag                                 >  2:1414095/1‑98 (MQ=255)
                                                              gagaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCg                                 >  2:2164579/1‑100 (MQ=255)
                                                                gaTTGAAGGCTTGATCAGTGAATCAGTGAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGt                                >  1:2915292/1‑99 (MQ=255)
                                                                gaTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTc                               >  2:3189640/1‑100 (MQ=255)
                                                                 aTTGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcg                              <  2:2977254/100‑1 (MQ=255)
                                                                  ttGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgc                             <  2:61250/100‑1 (MQ=255)
                                                                   tGAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTcgcg                            <  2:3215627/100‑1 (MQ=255)
                                                                    gAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGa                           >  1:1063478/1‑100 (MQ=255)
                                                                    gAAGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGa                           >  1:369137/1‑100 (MQ=255)
                                                                     aaGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAg                          <  1:1211098/100‑1 (MQ=255)
                                                                      aGGCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAgt                         <  2:3379581/100‑1 (MQ=255)
                                                                       ggCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAgtg                        >  2:1862096/1‑100 (MQ=255)
                                                                        gCTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAgtgt                       >  2:1166744/1‑100 (MQ=255)
                                                                         cTTGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTc                      <  2:3409367/100‑1 (MQ=255)
                                                                          ttGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTca                     <  2:2780638/100‑1 (MQ=255)
                                                                           tGATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAATCCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcac                    <  1:1863256/100‑1 (MQ=255)
                                                                            gATCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcaca                   <  2:2922905/100‑1 (MQ=255)
                                                                             aTCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacac                  <  1:2750329/100‑1 (MQ=255)
                                                                             aTCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacac                  <  2:2876439/100‑1 (MQ=255)
                                                                              tCAGTGAATCAGTCAGGTTGATTGACCTGGGGTTGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATACCGTCGCGAGTGTcacaca                 <  1:1952622/100‑1 (MQ=255)
                                                                              tCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacaca                 <  1:2202389/100‑1 (MQ=255)
                                                                              tCAGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTcacaca                 <  1:662819/100‑1 (MQ=255)
                                                                                aGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACata               <  1:676848/100‑1 (MQ=255)
                                                                                aGTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACata               >  1:800251/1‑100 (MQ=255)
                                                                                 gTGAATCAGTCAGGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATGGTTGATGCCGTCGCGAGTGTCACACata               >  1:3402673/1‑99 (MQ=255)
                                                                                      tgagtcagTTTGATTGACCTTGGTTCGGATGAGGTTGCATCGGGCGGGAAAACCATGAGCACTATTGTTGATGCGGTCGCGAGTGTCACACATATCATGc         <  1:846590/98‑1 (MQ=255)
                                                                                      tcagtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGc         >  1:2906430/1‑100 (MQ=255)
                                                                                       cagtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCa        <  1:1720146/100‑1 (MQ=255)
                                                                                       cagtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCa        <  1:2605811/100‑1 (MQ=255)
                                                                                        agtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAg       <  2:2664561/100‑1 (MQ=255)
                                                                                         gtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg      <  1:1402349/100‑1 (MQ=255)
                                                                                         gtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg      <  2:2739362/100‑1 (MQ=255)
                                                                                         gtcagGTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAgg      >  1:3337004/1‑100 (MQ=255)
                                                                                             ggTTGATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAt  >  2:2751289/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GATTTGCCGTTGTCGCCAGCGAAGTACGGACACTCGCAAGTCGCAGCGCTCAGGCGGCGAAAGAGATTGAAGGCTTGATCAGTGAATCAGTCAGGTTAATTGACCTGGGGTCGGATGAGGTGGCAACGGCCGGGAAAACCATGAGCACTATTGTTGATGCCGTCGCGAGTGTCACACATATCATGCAGGAAAT  >  NC_000913/1493677‑1493869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑