Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,547,395 A→G intergenic (‑226/‑6) yddG ← / → fdnG aromatic amino acid exporter/formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,547,3950AG99.3% 520.4 / ‑5.1 151intergenic (‑226/‑6)yddG/fdnGaromatic amino acid exporter/formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (68/81);  total (69/82)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 151 total aligned reads displayed.

TTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAA  >  NC_000913/1547299‑1547490
                                                                                                |                                                                                               
  ttttgGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCa                                                                                            >  2:1515314/4‑100 (MQ=255)
    gtgGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAAt                                                                                          >  2:214912/1‑100 (MQ=255)
      ggCAGACAGAAGTTCTTTTAGATTTCGCGCAAAAAGAGTCAGAATTTTACTGTTATGTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATgg                                                                                        >  1:2899176/1‑100 (MQ=255)
      ggCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATgg                                                                                        <  1:1468283/100‑1 (MQ=255)
       gCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGTGAAGCAATGGa                                                                                       >  1:2075522/1‑100 (MQ=255)
       gCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGa                                                                                       <  2:3261321/100‑1 (MQ=255)
       gCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGa                                                                                       >  2:2461598/1‑100 (MQ=255)
        cgaaCAGATGTTCTTTTGATTTCGCGCAAAAAGATTCAGAATATTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACg                                                                                     >  2:2351034/3‑100 (MQ=255)
        cAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGAc                                                                                      <  2:2297798/100‑1 (MQ=255)
         aaaCAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACg                                                                                     <  1:2259578/100‑1 (MQ=255)
          aaCAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCCGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGt                                                                                    <  2:2443031/100‑1 (MQ=255)
           aCAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTc                                                                                   <  1:1287549/100‑1 (MQ=255)
           aCAGATGTTCTTTTTGATTTCGCACAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTc                                                                                   <  1:3348831/100‑1 (MQ=255)
                tGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCg                                                                              >  2:543824/1‑100 (MQ=255)
                tGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCg                                                                              >  2:541429/1‑100 (MQ=255)
                tGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCg                                                                              <  2:2856103/100‑1 (MQ=255)
                 gTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGc                                                                             >  1:714666/1‑100 (MQ=255)
                 gTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGc                                                                             >  2:1282121/1‑100 (MQ=255)
                  ttCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCa                                                                            <  1:519850/100‑1 (MQ=255)
                  ttCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCa                                                                            <  1:825846/100‑1 (MQ=255)
                  ttCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCa                                                                            <  1:717980/100‑1 (MQ=255)
                   tCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAg                                                                           <  1:1169666/100‑1 (MQ=255)
                   tCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAg                                                                           <  2:124928/100‑1 (MQ=255)
                   tCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAg                                                                           >  1:2808076/1‑100 (MQ=255)
                     tttttGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGAc                                                                         >  2:3375188/1‑100 (MQ=255)
                          gATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAttt                                                                    >  1:370817/1‑100 (MQ=255)
                             ttCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttttt                                                                 >  2:3397483/1‑100 (MQ=255)
                             ttCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttttt                                                                 >  1:3216986/1‑100 (MQ=255)
                             ttCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAAtttttt                                                                 <  2:1054500/100‑1 (MQ=255)
                                    aaaaaGATTCAGAATTTTACTGTTAGATTCCTCGGTGAGTAATACTCGTTAAAAAAGAGAGACGCAATGGACGTTAGTCGCAGACAATGTAATaaaa                                                             >  1:2004365/1‑97 (MQ=255)
                                     aaaaGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTg                                                         >  2:3223838/1‑100 (MQ=255)
                                       aaGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTgcg                                                       >  1:301673/1‑100 (MQ=255)
                                        aGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTgcgc                                                      <  1:2530085/100‑1 (MQ=255)
                                        aGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTgcgc                                                      <  2:597052/100‑1 (MQ=255)
                                        aGATTCAGAATGTTACTGTTAGTTTCCTCGCGCAGTAATAAAAAAAAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTgcgc                                                      <  1:2891285/100‑1 (MQ=255)
                                         gATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGcgcg                                                     >  2:2620374/1‑100 (MQ=255)
                                         gATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGcgcg                                                     <  1:543818/100‑1 (MQ=255)
                                          aTTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgg                                                    >  1:2721293/1‑100 (MQ=255)
                                          aTTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgg                                                    >  1:1394713/1‑100 (MQ=255)
                                            tCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggc                                                  >  2:1884292/1‑100 (MQ=255)
                                            tCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggc                                                  <  2:2213815/100‑1 (MQ=255)
                                             cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg                                                 >  1:110339/1‑100 (MQ=255)
                                             cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg                                                 >  1:536834/1‑100 (MQ=255)
                                             cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcgggcg                                                 >  1:3159045/1‑100 (MQ=255)
                                             cAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCgcggg                                                   >  1:25115/1‑98 (MQ=255)
                                                aaTTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTa                                              <  1:1347399/100‑1 (MQ=255)
                                                       cTGTTAGTTTCCTCGTGCAGTAATACCCCCGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGCCAATTTTTTAAAATCTGCGCGGGCGGTAATGCTgg                                       <  1:3015285/100‑1 (MQ=255)
                                                       cTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTgg                                       <  2:8677/100‑1 (MQ=255)
                                                       cTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTgg                                       <  1:775355/100‑1 (MQ=255)
                                                        tGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGTAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGa                                      <  2:585008/100‑1 (MQ=255)
                                                         gTTAGTTTCCTCGCGCATTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGa                                      <  2:2899222/99‑1 (MQ=255)
                                                          ttAGTTTCCTCGCGCAGTAATACCCCTGATAAAAGAGAGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaac                                    <  1:2098265/100‑1 (MQ=255)
                                                          ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaac                                    <  2:194696/100‑1 (MQ=255)
                                                          ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaac                                    <  1:1536754/100‑1 (MQ=255)
                                                          ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaac                                    >  1:1804609/1‑100 (MQ=255)
                                                          ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaac                                    <  2:370822/100‑1 (MQ=255)
                                                          ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGa                                      <  2:2654464/98‑1 (MQ=255)
                                                           tAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaaca                                   <  2:1370247/100‑1 (MQ=255)
                                                            aGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaacaa                                  >  1:1935795/1‑100 (MQ=255)
                                                             gTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaacaac                                 >  2:2457594/1‑100 (MQ=255)
                                                             gTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaacaac                                 <  1:2658048/100‑1 (MQ=255)
                                                             gTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGaacaac                                 >  2:1908508/1‑100 (MQ=255)
                                                              tttCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAacaaca                                >  1:243335/1‑100 (MQ=255)
                                                               ttCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAg                               <  2:3006253/100‑1 (MQ=255)
                                                                tCCTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGt                              <  2:2588250/100‑1 (MQ=255)
                                                                  cTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAg                            >  2:1377658/1‑100 (MQ=255)
                                                                  cTCGCGCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAg                            >  2:1342936/1‑100 (MQ=255)
                                                                    cgcgCAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCg                          <  2:1163575/100‑1 (MQ=255)
                                                                      cgcAGTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCgac                        <  2:1394176/100‑3 (MQ=255)
                                                                          gTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTg                    >  2:716813/1‑100 (MQ=255)
                                                                          gTAATACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTg                    >  1:2472308/1‑100 (MQ=255)
                                                                             aTACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGc                 <  2:259776/100‑1 (MQ=255)
                                                                              tACCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCt                <  2:3021851/100‑1 (MQ=255)
                                                                               aCCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCTCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCtt               <  2:3313267/100‑1 (MQ=255)
                                                                               aCCCCTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCtt               <  2:1581830/100‑1 (MQ=255)
                                                                                ccccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCttt              >  1:2061834/1‑100 (MQ=255)
                                                                                ccccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCttt              <  2:1176226/100‑1 (MQ=255)
                                                                                ccccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCttt              >  1:2661463/1‑100 (MQ=255)
                                                                                 cccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCGGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTg             >  2:1427956/1‑100 (MQ=255)
                                                                                 cccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTg             >  1:366886/1‑100 (MQ=255)
                                                                                  ccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGc            >  2:1591390/1‑100 (MQ=255)
                                                                                  ccTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGc            <  1:2236545/100‑1 (MQ=255)
                                                                                   cTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcc           <  1:1122440/100‑1 (MQ=255)
                                                                                   cTGAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcc           <  1:1842717/100‑1 (MQ=255)
                                                                                     gAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcccc         >  1:885945/1‑100 (MQ=255)
                                                                                     gAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcccc         >  1:2216646/1‑100 (MQ=255)
                                                                                     gAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcccc         <  1:1905189/100‑1 (MQ=255)
                                                                                     gAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcccc         >  2:2228769/1‑100 (MQ=255)
                                                                                     gAAAAAAGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGcccc         >  2:719807/1‑100 (MQ=255)
                                                                                       aaaaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGa       <  1:332981/100‑1 (MQ=255)
                                                                                       aaaaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGa       >  2:1754260/1‑100 (MQ=255)
                                                                                        aaaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGTCCCGaa      <  1:1270245/100‑1 (MQ=255)
                                                                                        aaaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaa      <  2:1736146/100‑1 (MQ=255)
                                                                                         aaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGGGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaag     <  2:2371988/100‑1 (MQ=255)
                                                                                         aaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaag     <  1:54382/100‑1 (MQ=255)
                                                                                         aaaGAGGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaag     <  1:1531040/100‑1 (MQ=255)
                                                                                           agagGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaagca   <  1:2351002/100‑1 (MQ=255)
                                                                                           agagGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaagca   <  2:3317820/100‑1 (MQ=255)
                                                                                           agagGGAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaagca   >  2:355916/1‑100 (MQ=255)
                                                                                            gagGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGaagcaa  >  2:1606895/1‑100 (MQ=255)
                                                                                                |                                                                                               
TTTTGTGGCAAACAGATGTTCTTTTTGATTTCGCGCAAAAAGATTCAGAATTTTACTGTTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAA  >  NC_000913/1547299‑1547490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: