Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,555,324 A→G S116P (TCT→CCT)  maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,3240AG98.5% 459.7 / NA 133S116P (TCT→CCT) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (63/67);  minor base C (0/2);  total (63/70)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTT  >  NC_000913/1555227‑1555420
                                                                                                 |                                                                                                
ttAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGgaa                                                                                                <  1:2639528/100‑1 (MQ=255)
  aaTATTATGGTTCGGCACGTGTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGgaa                                                                                                <  2:427114/98‑1 (MQ=255)
    tattatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACg                                                                                            >  1:3110594/1‑100 (MQ=255)
     attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGc                                                                                           <  1:3383894/100‑1 (MQ=255)
     attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGc                                                                                           >  2:170281/1‑100 (MQ=255)
      ttatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCt                                                                                          <  2:1689788/100‑1 (MQ=255)
        atGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCCCGTGCACGGCGGTAGATCTCAGGAAAACGCTc                                                                                         >  1:837308/1‑99 (MQ=255)
         tGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcac                                                                                       <  2:1664262/100‑1 (MQ=255)
          ggTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTcaca                                                                                      >  1:2890447/1‑100 (MQ=255)
           gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAg                                                                                     <  1:369023/100‑1 (MQ=255)
               ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg                                                                                 >  1:90823/1‑100 (MQ=255)
               ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTg                                                                                 >  2:1975007/1‑100 (MQ=255)
                gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgc                                                                                >  1:249005/1‑100 (MQ=255)
                gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTgc                                                                                <  2:2736809/100‑1 (MQ=255)
                    gTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCg                                                                            >  1:844692/1‑100 (MQ=255)
                       ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACg                                                                         >  1:462615/1‑100 (MQ=255)
                       ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACg                                                                         <  1:3270915/100‑1 (MQ=255)
                        tGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACgg                                                                        >  1:587953/1‑100 (MQ=255)
                         gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACgg                                                                        >  1:3185060/1‑99 (MQ=255)
                          cAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGtt                                                                      <  2:2655733/100‑1 (MQ=255)
                           aGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTg                                                                     <  2:2701325/100‑1 (MQ=255)
                             ccgatcttCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGACGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTggg                                                                   <  1:2228894/93‑1 (MQ=255)
                             aaTATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTggg                                                                   <  2:587960/100‑1 (MQ=255)
                             aaTATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTggg                                                                   <  1:603748/100‑1 (MQ=255)
                             aaTATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTggg                                                                   <  1:1388980/100‑1 (MQ=255)
                              atatCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTgggg                                                                  <  2:1131049/100‑1 (MQ=255)
                              atatCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTgggg                                                                  <  2:2009030/100‑1 (MQ=255)
                               tctcgtcgATCTGGTGCCGGGTCTGGTAAGAGATAAGCACGCCGCGTGAACGGCGGTAGATCTCAGGTAAACGCGCACAGGCTGCGCCGACGGTTGGGGt                                                                 <  1:2776256/98‑1 (MQ=255)
                                atCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGta                                                                <  2:1302850/100‑1 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACCCGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              <  1:138779/100‑1 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              <  2:1216316/100‑1 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              >  2:509215/1‑100 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGtata                                                              >  2:2213327/1‑100 (MQ=255)
                                  cGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCCCACAGGCTGCGCCGACGGTTGGGGtata                                                              >  2:36315/1‑100 (MQ=255)
                                   gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataa                                                             >  1:185808/1‑100 (MQ=255)
                                   gTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGAGCCGACGGTTGGGGTataa                                                             >  1:1146193/1‑100 (MQ=255)
                                    tCCATATTGTGCCGGTCCTGGTAAGAGATAAGCACGCCGCGTGACCGGCGGTAGGTCTCAGCAAAACACTCGCAGCGCGCGCCGACGCTTGGGGtatagc                                                            <  1:3398441/100‑3 (MQ=255)
                                         atTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACa                                                       <  2:1370974/100‑1 (MQ=255)
                                          ttGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAg                                                      <  1:1534266/100‑1 (MQ=255)
                                            gtgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGc                                                    <  1:3364971/100‑1 (MQ=255)
                                              gCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcat                                                  >  1:1976395/1‑100 (MQ=255)
                                               ccGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatc                                                 <  2:2266918/100‑1 (MQ=255)
                                                 ggTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGAAGGTTGGGGTATAAATAACAGGcatcat                                               >  2:1159382/1‑100 (MQ=255)
                                                 ggTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCAGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatcat                                               >  2:2442964/1‑100 (MQ=255)
                                                   ttCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatct                                             <  2:1759575/100‑1 (MQ=255)
                                                   ttCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatct                                             >  1:3442010/1‑100 (MQ=255)
                                                     cTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctca                                           <  2:2656034/100‑1 (MQ=255)
                                                      tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                          <  2:3473196/100‑1 (MQ=255)
                                                      tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                          >  2:3044476/1‑100 (MQ=255)
                                                      tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                          >  1:1100766/1‑100 (MQ=255)
                                                      tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcan                                          <  1:869500/100‑2 (MQ=255)
                                                       ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                         >  1:1019379/1‑100 (MQ=255)
                                                       ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                         >  1:1370831/1‑100 (MQ=255)
                                                       ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                         >  1:1652341/1‑100 (MQ=255)
                                                       cttccgatctAAACACGCCGCGTGAACGGCTGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                         <  1:265347/91‑1 (MQ=255)
                                                        gTAAGAGATAAACACGCCGCGTGAACGGCGGTATATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                          <  1:2941022/98‑1 (MQ=255)
                                                        gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca                                        <  2:141147/100‑1 (MQ=255)
                                                        gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca                                        >  2:2546402/1‑100 (MQ=255)
                                                        gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGAGACGACGGTTGGGGTATAAATAACAAGCAtcatccca                                           >  1:2739826/1‑97 (MQ=255)
                                                          aaGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAg                                      >  2:396899/1‑100 (MQ=255)
                                                           agagATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCatcatccc                                            >  1:3152911/1‑91 (MQ=255)
                                                           agagATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAga                                     >  2:813180/1‑100 (MQ=255)
                                                           agagATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAga                                     <  1:1207670/100‑1 (MQ=255)
                                                            gagaTAAACACGCCGCGTGAACTGTGGTAGACCTCAGTAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat                                    <  1:2338050/100‑1 (MQ=255)
                                                            gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat                                    <  2:196871/100‑1 (MQ=255)
                                                            gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat                                    <  2:3231956/100‑1 (MQ=255)
                                                            gagaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgat                                    >  2:129780/1‑100 (MQ=255)
                                                             agaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatg                                   <  2:1898712/100‑1 (MQ=255)
                                                               aTAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatgat                                 >  2:53060/1‑100 (MQ=255)
                                                                tAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                >  2:2321721/1‑100 (MQ=255)
                                                                tAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                <  1:3477047/100‑1 (MQ=255)
                                                                 aaaCACGCCGCGTGAACGGCTGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatcagatcggaag                               >  2:265351/1‑91 (MQ=255)
                                                                 aaaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg                               <  1:1379960/100‑1 (MQ=255)
                                                                 aaaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTg                               >  1:1555548/1‑100 (MQ=255)
                                                                  aaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGt                              >  1:1232646/1‑100 (MQ=255)
                                                                  aaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGt                              >  1:273027/1‑100 (MQ=255)
                                                                  aaCACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGt                              >  1:2471627/1‑100 (MQ=255)
                                                                      cgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc                          <  1:1861641/100‑1 (MQ=255)
                                                                       gccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                         <  2:713515/100‑1 (MQ=255)
                                                                       gccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                         <  1:2469438/100‑1 (MQ=255)
                                                                           cgTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGAc                     >  1:3219630/1‑100 (MQ=255)
                                                                             tGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACgg                   <  1:2546368/100‑1 (MQ=255)
                                                                               aaCGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGATGGGGGATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTa                 >  1:1204820/1‑100 (MQ=255)
                                                                                 cggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTaga               >  2:2267338/1‑100 (MQ=255)
                                                                                 cggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTaga               <  2:3201455/100‑1 (MQ=255)
                                                                                 cggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTaga               <  1:2938867/100‑1 (MQ=255)
                                                                                  ggcggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaa              <  2:2471660/100‑1 (MQ=255)
                                                                                    cggTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaag             <  1:1331452/99‑1 (MQ=255)
                                                                                      gTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAgg          >  2:1567071/1‑100 (MQ=255)
                                                                                      gTAGATCTCAGCAAAACACTCCCAGGCTGCGCCGACGGGCAGGGTATAAATAACACGCATCATCTCATAAAGACTATTGTTTAACAGACGGTAGAACAgg          <  1:1436630/100‑1 (MQ=255)
                                                                                       tAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg         <  2:257010/100‑1 (MQ=255)
                                                                                        tgATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGt        <  2:1979978/99‑1 (MQ=255)
                                                                                         gATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGta       >  1:1979949/1‑99 (MQ=255)
                                                                                          aTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCGTCAAGATGATTGTTGACCAGACGGTAGAAGAGGGttt      >  1:2341026/1‑100 (MQ=255)
                                                                                          aTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGttt      >  2:2231347/1‑100 (MQ=255)
                                                                                           tctcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTc     >  2:33857/1‑100 (MQ=255)
                                                                                            ctcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg    >  2:3031335/1‑100 (MQ=255)
                                                                                             tcAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGt   >  2:2957985/1‑100 (MQ=255)
                                                                                              cAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGtt  >  2:2095142/1‑100 (MQ=255)
                                                                                              cAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGt   >  1:1886155/1‑99 (MQ=255)
                                                                                                 |                                                                                                
TTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTT  >  NC_000913/1555227‑1555420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: