Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,827,031 T→A D410V (GAT→GTT)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,0310TA99.2% 415.0 / NA 125D410V (GAT→GTT) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (58/66);  minor base C (0/1);  total (58/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCC  >  NC_000913/1826934‑1827126
                                                                                                 |                                                                                               
ctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                               <  2:3120355/100‑1 (MQ=255)
 tcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACgg                                                                                              <  2:722402/100‑1 (MQ=255)
  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCCGCAATTGCGGGTCGGCCAGAACGgc                                                                                             >  2:2637752/1‑100 (MQ=255)
    cgcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGGCGGCCAGAACGgc                                                                                             <  1:2144908/98‑1 (MQ=255)
     gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                          <  2:1071132/100‑1 (MQ=255)
     gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                          <  2:823758/100‑1 (MQ=255)
      cTGGAACGGATAAACCGAACCTAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagca                                                                                         >  2:3138919/1‑100 (MQ=255)
      cTGGAACGGATAAACCGAACCGAGATTCAGTTATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagca                                                                                         <  1:1123669/100‑1 (MQ=255)
       tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                        <  2:769998/100‑1 (MQ=255)
          aaCGGATAAACCGGACCGAGATTCAGTAATTGGCTCAATACATCCAGGGCTTCCCGCCATTGGCGCAGCAATTGTGGGCCGGCCAGAACGGCAGCAGCaa                                                                                     >  1:2147509/1‑100 (MQ=255)
          aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTaa                                                                                     >  2:2002619/1‑100 (MQ=255)
          aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTaa                                                                                     >  1:2452333/1‑100 (MQ=255)
          aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCCTCCCCCACTTCGCGCAGCAATTGTCGGTCGGCCAGAACGGCAGCAGCaa                                                                                     >  1:777302/1‑100 (MQ=255)
            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                   >  2:106456/1‑100 (MQ=255)
            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                   <  2:125332/100‑1 (MQ=255)
            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                   <  1:109475/100‑1 (MQ=255)
            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                   <  1:1474426/100‑1 (MQ=255)
             ggATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc                                                                                  <  1:2706818/100‑1 (MQ=255)
                  aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcg                                                                             >  2:469685/1‑100 (MQ=255)
                   accgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgc                                                                            <  2:1462162/100‑1 (MQ=255)
                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                          <  2:678515/100‑1 (MQ=255)
                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                          <  2:2933910/100‑1 (MQ=255)
                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                          <  1:1284975/100‑1 (MQ=255)
                        accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtag                                                                       <  2:2896359/100‑1 (MQ=255)
                        accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtag                                                                       <  2:901615/100‑1 (MQ=255)
                        accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtag                                                                       <  2:2381072/100‑1 (MQ=255)
                        accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtag                                                                       <  2:2130284/100‑1 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                      >  2:1458196/1‑100 (MQ=255)
                          cgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagta                                                                     <  2:612673/100‑1 (MQ=255)
                           gagaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTaa                                                                    >  2:1525465/1‑100 (MQ=255)
                             gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCGCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCTCGGTAGTAACg                                                                  <  1:1825852/100‑1 (MQ=255)
                             gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACg                                                                  >  1:3017513/1‑100 (MQ=255)
                             gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACg                                                                  >  2:2168642/1‑100 (MQ=255)
                             gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACg                                                                  >  2:505693/1‑100 (MQ=255)
                               ttCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGAt                                                                >  1:783465/1‑100 (MQ=255)
                                tCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATc                                                               <  2:281382/100‑1 (MQ=255)
                                 cAGTAATTGGCTCAATACTTCCAGTGCTTCCCGCCCTTCGCGCAGCAATGGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc                                                              <  1:2385628/100‑1 (MQ=255)
                                   gTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATccca                                                            >  2:2688506/1‑98 (MQ=255)
                                   gTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAc                                                            >  2:1794405/1‑100 (MQ=255)
                                    tAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGCTCGCGGTAGTAACGATCCAc                                                            <  1:2131817/99‑1 (MQ=255)
                                      aTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCa                                                         >  2:191022/1‑100 (MQ=255)
                                      aTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCa                                                         >  2:1849992/1‑100 (MQ=255)
                                      aTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCa                                                         >  1:1329296/1‑100 (MQ=255)
                                        tGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGt                                                       >  2:1482492/1‑100 (MQ=255)
                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc                                                      >  1:8172/1‑100 (MQ=255)
                                          gCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCa                                                     <  2:1413557/100‑1 (MQ=255)
                                          gCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCa                                                     >  2:91007/1‑100 (MQ=255)
                                          gCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCa                                                     >  1:1692248/1‑100 (MQ=255)
                                           cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt                                                    >  1:2529651/1‑100 (MQ=255)
                                           cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt                                                    >  2:874132/1‑100 (MQ=255)
                                            tCAATACATCCAGTGCTTCCCGCCCGTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAtt                                                   <  1:2930373/100‑1 (MQ=255)
                                             cAATACATCCAGCGCTTCCCGCCCTTCGCGCAGCAATTGGGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAtt                                                   <  2:777313/99‑1 (MQ=255)
                                              aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAGCGATCTACGCAGGCATTGa                                                 >  2:2406461/1‑100 (MQ=255)
                                               aTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGACCGGCAGCAGTACGGCGATCGCGGTCATAAAGATCCACACAGTCATTgcg                                                <  1:510121/100‑3 (MQ=255)
                                                tACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgc                                               >  2:2903719/1‑100 (MQ=255)
                                                tACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgc                                               <  2:2433640/100‑1 (MQ=255)
                                                 aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg                                              <  1:3058131/100‑1 (MQ=255)
                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                             >  1:2452775/1‑100 (MQ=255)
                                                   aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGAGTCGGCCAGAACGGCCGCAGTAAGGCGATCGCGGTAGTAACAATCCACCCAGTCATCGGgcg                                              >  1:2844408/1‑98 (MQ=255)
                                                    tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt                                           >  2:478125/1‑100 (MQ=255)
                                                    tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt                                           >  1:411669/1‑100 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                         <  2:650945/100‑1 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                         <  1:946121/100‑1 (MQ=255)
                                                        gTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCACCCAGTCATTGAGCGCATTAAAc                                      <  1:469680/100‑1 (MQ=255)
                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTTGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACa                                     <  2:181516/100‑1 (MQ=255)
                                                           cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                    >  1:2167138/1‑100 (MQ=255)
                                                           cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                    >  1:2122194/1‑100 (MQ=255)
                                                            ttCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGc                                   <  2:411674/100‑1 (MQ=255)
                                                            ttCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGc                                   <  1:492593/100‑1 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                  >  1:3002469/1‑100 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                  <  1:1525443/100‑1 (MQ=255)
                                                                cGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt                               >  2:2709607/1‑100 (MQ=255)
                                                                 gCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATc                              <  1:3138869/100‑1 (MQ=255)
                                                                   ccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                            >  2:1947632/1‑100 (MQ=255)
                                                                    cTTCGCGCAGCATTTGCTGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGTATTAAACTGCGTATCGtt                           <  1:819957/100‑1 (MQ=255)
                                                                    cTTCGCGCAGCAATTGCGGGTCGGCCCGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGtt                           >  2:2114366/1‑100 (MQ=255)
                                                                    cTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGtt                           <  2:2684360/100‑1 (MQ=255)
                                                                     ttCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtc                          <  1:1556527/100‑1 (MQ=255)
                                                                     ttCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtc                          <  2:3191894/100‑1 (MQ=255)
                                                                     ttCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtc                          <  1:3416039/100‑1 (MQ=255)
                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                        >  2:2296373/1‑100 (MQ=255)
                                                                        gcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatc                       <  2:1678767/100‑1 (MQ=255)
                                                                        gcgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatc                       <  2:2404882/100‑1 (MQ=255)
                                                                         cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                      <  2:1285035/100‑1 (MQ=255)
                                                                          gcagcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcat                     >  1:1693916/1‑100 (MQ=255)
                                                                             gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc                  >  1:2768117/1‑100 (MQ=255)
                                                                             gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc                  >  1:2049740/1‑100 (MQ=255)
                                                                                aTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc               <  1:2750673/100‑1 (MQ=255)
                                                                                  tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg             <  2:2260253/100‑1 (MQ=255)
                                                                                  tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg             <  1:1551391/100‑1 (MQ=255)
                                                                                    cGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGa           <  1:296452/100‑1 (MQ=255)
                                                                                     gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt          <  1:226615/100‑1 (MQ=255)
                                                                                      ggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAtt         <  2:489469/100‑1 (MQ=255)
                                                                                         cGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAc      <  2:774431/100‑1 (MQ=255)
                                                                                         cGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAc      >  2:104954/1‑100 (MQ=255)
                                                                                          ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc     >  2:3348211/1‑100 (MQ=255)
                                                                                          ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc     <  1:505688/100‑1 (MQ=255)
                                                                                           gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg    >  2:1223623/1‑100 (MQ=255)
                                                                                           gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg    <  1:1808186/100‑1 (MQ=255)
                                                                                             cAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATGGAGCGCAGTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGcc  >  1:2075754/1‑100 (MQ=255)
                                                                                                 |                                                                                               
CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCC  >  NC_000913/1826934‑1827126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: