Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,071,321 A→G intergenic (+110/‑218) yeeP → / → flu pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,071,3210AG98.6% 498.5 / ‑3.2 148intergenic (+110/‑218)yeeP/flupseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (72/73);  total (73/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 150 total aligned reads displayed.

TGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAAC  >  NC_000913/2071224‑2071417
                                                                                                 |                                                                                                
 gATGCCCTCCCTGACTCTGAGTCTGCTCATAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTTCAATAGCTCAATAATAGAATAAAACGATCGata                                                                                               <  1:2092807/100‑1 (MQ=255)
   tGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATc                                                                                             >  2:355152/1‑100 (MQ=255)
     ncctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTa                                                                                           >  1:2480390/2‑100 (MQ=255)
     ccctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTa                                                                                           >  1:1759703/1‑100 (MQ=255)
      cctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAt                                                                                          >  1:1139249/1‑100 (MQ=255)
       ctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAtt                                                                                         >  1:980197/1‑100 (MQ=255)
        tccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAttt                                                                                        <  2:3158172/100‑1 (MQ=255)
          cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTa                                                                                      <  2:811720/100‑1 (MQ=255)
          cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTa                                                                                      >  2:2773274/1‑100 (MQ=255)
             gACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcg                                                                                   <  2:560149/100‑1 (MQ=255)
             gACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCACAATAATAGAATAAAACGATCGATATCTATTTTatcg                                                                                   <  1:1868639/100‑1 (MQ=255)
               ctctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGACTCAAACGATCGATCTCTATTTTatc                                                                                    >  1:978813/1‑97 (MQ=255)
               ctctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcga                                                                                  <  1:2099554/99‑1 (MQ=255)
                  tGAGTCTTCTGACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcga                                                                                  <  1:1991852/96‑1 (MQ=255)
                  tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGt                                                                              >  1:654701/1‑100 (MQ=255)
                  tGAGTCTGCTCACAAAAGCACTGTTTGCGTTACTGTATCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGt                                                                              <  2:2100380/100‑1 (MQ=255)
                     gTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTta                                                                           <  2:3406933/100‑1 (MQ=255)
                       cTGCTTACAAAAGCTTTGTTTGCGGTACTGTCTCTCTTGTCCGTGCAATAGTTCAATAATAGAATAAAACGATCGATCTCTCTTTTATCGATCGttt                                                                            <  1:1505999/97‑1 (MQ=255)
                       cTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTtata                                                                         >  2:3252826/1‑100 (MQ=255)
                       cTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTtata                                                                         <  2:43200/100‑1 (MQ=255)
                        tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTatat                                                                        <  1:58911/100‑1 (MQ=255)
                        tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTatat                                                                        >  1:3098220/1‑100 (MQ=255)
                        tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTatat                                                                        >  1:2923715/1‑100 (MQ=255)
                        tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGAACGATATCTATGTTatggattatttta                                                                          >  2:2129451/1‑91 (MQ=255)
                         gCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatc                                                                       >  2:944739/1‑100 (MQ=255)
                         gCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatc                                                                       >  1:1153433/1‑100 (MQ=255)
                           tCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcga                                                                     >  2:2641313/1‑100 (MQ=255)
                            cacaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgat                                                                    >  1:2471501/1‑100 (MQ=255)
                             acaAAAGCACTGTTTTTGTTACTGTATCTCTTGTTCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgatc                                                                   <  1:1204560/100‑1 (MQ=255)
                             acaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgatc                                                                   <  1:925517/100‑1 (MQ=255)
                                aaaTCACTTTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATcgatcgat                                                                <  1:142428/100‑1 (MQ=255)
                                aaaGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATcgatcgat                                                                >  2:873386/1‑100 (MQ=255)
                                   gCACTGTTTTCGTTACTGTCTCTCTTGTCCGTTCAATAGTTTAATAATAGAATACAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATa                                                               <  1:2989216/98‑1 (MQ=255)
                                   gCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAg                                                             <  1:1592362/100‑1 (MQ=255)
                                    cACTGTTTTCGTTACCGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGc                                                            >  1:3282030/1‑100 (MQ=255)
                                        gTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaat                                                        >  2:2379271/1‑100 (MQ=255)
                                        gTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaat                                                        >  2:2613892/1‑100 (MQ=255)
                                        gTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATAGTTTATAACGATCGATACACtaat                                                        >  1:2769327/1‑100 (MQ=255)
                                         ttttCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaata                                                       <  1:777473/100‑1 (MQ=255)
                                            tCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataata                                                    <  1:1230531/100‑1 (MQ=255)
                                            tCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataata                                                    >  2:1344288/1‑100 (MQ=255)
                                                 aCTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCtt                                               >  2:1276954/1‑100 (MQ=255)
                                                   tGTCTCTCTTGTCCGTGCAATAGCTTAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                             <  1:2447634/100‑1 (MQ=255)
                                                   tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                             >  2:2491852/1‑100 (MQ=255)
                                                   tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                             >  1:3442998/1‑100 (MQ=255)
                                                   tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                             <  2:1153448/100‑1 (MQ=255)
                                                   tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATAACGATCGATAAGCTAATAATAACCttt                                              >  1:1648388/1‑99 (MQ=255)
                                                   tGTCTCTCTTGTCCGTGCAATAGCCCAATAATAGAATAAAACGATCGATATCTATTTTATCGCTCGTTTATATCGATCGATCAGCTAATAATAACCTTTg                                             >  2:2901074/1‑100 (MQ=255)
                                                    gTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGt                                            >  2:2217220/1‑100 (MQ=255)
                                                     tctctcTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTc                                           >  2:20007/1‑100 (MQ=255)
                                                      ctctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                          <  2:1643954/100‑1 (MQ=255)
                                                      ctctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                          <  1:2773230/100‑1 (MQ=255)
                                                       tctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAg                                         <  2:2842955/100‑1 (MQ=255)
                                                       tctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAg                                         <  2:1757673/100‑1 (MQ=255)
                                                        ctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGt                                        >  1:2496697/1‑100 (MQ=255)
                                                        ctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGt                                        <  2:2644997/100‑1 (MQ=255)
                                                         tctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTa                                       <  1:202507/100‑1 (MQ=255)
                                                           ttGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAAc                                     <  1:584862/100‑1 (MQ=255)
                                                           ttGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAAc                                     <  2:1643387/100‑1 (MQ=255)
                                                            tGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACa                                    <  2:368334/100‑1 (MQ=255)
                                                             gTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGCTCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACAt                                   >  1:686520/1‑100 (MQ=255)
                                                             gTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACAt                                   >  1:1754163/1‑100 (MQ=255)
                                                               ccGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGc                                 >  1:857077/1‑100 (MQ=255)
                                                                cGTGCAATAGCTCAATAATAGCATAAAACGATCCATATCTATTTTATCCATCGTTTATATCGATCGATAAGCTCATAATACCCTTTGTCAGTAACATGca                                <  1:2901028/100‑1 (MQ=255)
                                                                  tGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGcaca                              <  1:13245/100‑1 (MQ=255)
                                                                  tGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGcaca                              <  1:3420778/100‑1 (MQ=255)
                                                                   gCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAg                             <  2:2889916/100‑1 (MQ=255)
                                                                   gCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAg                             >  1:1833413/1‑100 (MQ=255)
                                                                        aGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACg                        <  2:1889718/100‑1 (MQ=255)
                                                                         gCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGt                       <  2:1789164/100‑1 (MQ=255)
                                                                          cTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTa                      >  1:2302784/1‑100 (MQ=255)
                                                                          cTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTa                      <  1:3271950/100‑1 (MQ=255)
                                                                          cTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTa                      >  2:3308381/1‑100 (MQ=255)
                                                                           tCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTAc                     <  2:404505/100‑1 (MQ=255)
                                                                            cAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACa                    >  2:2399721/1‑100 (MQ=255)
                                                                             aataatAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGAGCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTAc                     <  1:1131745/98‑1 (MQ=255)
                                                                              ataataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGa                  <  1:1162830/100‑1 (MQ=255)
                                                                               taataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTCGTAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaa                 >  1:923643/1‑100 (MQ=255)
                                                                               taataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaa                 >  1:1598127/1‑100 (MQ=255)
                                                                                aataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaaa                <  2:1144592/100‑1 (MQ=255)
                                                                                aataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaaa                <  2:1517652/100‑1 (MQ=255)
                                                                                aataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaaa                <  2:596205/100‑1 (MQ=255)
                                                                                aataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaaa                <  1:2217188/100‑1 (MQ=255)
                                                                                 ataGAATAACACGATCGATATCTATTTTATCGATCGTTTATATAAATCGATAAGCTAATAATAGCCTTAGACAGTAACATGCAAAAATACATACAGAAAg               >  1:2966801/1‑100 (MQ=255)
                                                                                   aGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGAc             >  2:33551/1‑100 (MQ=255)
                                                                                   aGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGAc             <  1:2391364/100‑1 (MQ=255)
                                                                                    gAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACa            >  2:3137981/1‑100 (MQ=255)
                                                                                    gAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACa            >  1:2200638/1‑100 (MQ=255)
                                                                                    gAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACa            >  2:258885/1‑100 (MQ=255)
                                                                                    gAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACa            >  2:152801/1‑100 (MQ=255)
                                                                                        aaaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCa        >  1:859090/1‑100 (MQ=255)
                                                                                         aaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCa        <  1:1274384/99‑1 (MQ=255)
                                                                                         aaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAg       <  2:1897440/100‑1 (MQ=255)
                                                                                         aaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAg       >  1:1643784/1‑100 (MQ=255)
                                                                                         aaaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGAAATTCAg       >  2:3116913/1‑100 (MQ=255)
                                                                                         aaaCGATCGATATATATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAg       <  2:2756322/100‑1 (MQ=255)
                                                                                          aaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAgg      <  1:801079/100‑1 (MQ=255)
                                                                                           aCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAggg     <  2:2021969/100‑1 (MQ=255)
                                                                                           aCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAggg     <  2:859101/100‑1 (MQ=255)
                                                                                              aTCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGaac  <  1:3245481/100‑1 (MQ=255)
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TGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAAC  >  NC_000913/2071224‑2071417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: