Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,159,354 C→T R323R (CGC→CGT)  mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,159,3540CT98.5% 430.2 / NA 131R323R (CGC→CGT) mdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (64/64);  minor base G (0/2);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGC  >  NC_000913/2159258‑2159447
                                                                                                |                                                                                             
cAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTcc                                                                                            >  2:3472609/1‑100 (MQ=255)
cAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTcc                                                                                            >  1:2976723/1‑100 (MQ=255)
   tattatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTccccc                                                                                         >  2:2194981/1‑100 (MQ=255)
     ttatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTccccccc                                                                                       >  2:3032462/1‑98 (MQ=255)
     ttatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccac                                                                                       <  2:933729/100‑1 (MQ=255)
     ttatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccac                                                                                       <  2:2573478/100‑1 (MQ=255)
     ttatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTACCCAGGATCGTTCCCccac                                                                                       <  2:1165440/100‑1 (MQ=255)
      tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccacc                                                                                      <  2:2300549/100‑1 (MQ=255)
       ttccgatctgTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccacca                                                                                     <  1:1205394/91‑1 (MQ=255)
        tCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCAt                                                                                    >  1:1130627/1‑100 (MQ=255)
            gACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTcgc                                                                                >  2:1246049/1‑100 (MQ=255)
             aCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTcgcg                                                                               <  2:1859196/100‑1 (MQ=255)
             aCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTcgcg                                                                               <  2:171789/100‑1 (MQ=255)
              cGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCgcgc                                                                              >  2:2727792/1‑100 (MQ=255)
                 ttGACAGCATCCGGGGAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTc                                                                           <  1:468469/100‑1 (MQ=255)
                   gACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGc                                                                         >  1:942256/1‑100 (MQ=255)
                   gACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGc                                                                         <  2:198877/100‑1 (MQ=255)
                    aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCCAATTGCCCCGGATCGTTCCCCCACCATTCGCGCCTCGCt                                                                        >  2:121985/1‑100 (MQ=255)
                      aGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTgg                                                                      <  1:414691/100‑1 (MQ=255)
                      aGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTgg                                                                      <  1:109177/100‑1 (MQ=255)
                       gCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCCCGCCTCGCTGga                                                                     <  2:2279752/100‑1 (MQ=255)
                        cATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaa                                                                    >  2:138179/1‑100 (MQ=255)
                        cATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaa                                                                    >  2:234875/1‑100 (MQ=255)
                        cATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaa                                                                    >  2:304559/1‑100 (MQ=255)
                         aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag                                                                   <  1:1768258/100‑1 (MQ=255)
                         aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag                                                                   <  2:2523790/100‑1 (MQ=255)
                         aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag                                                                   <  1:1194851/100‑1 (MQ=255)
                         aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCAGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag                                                                   <  2:1162172/100‑1 (MQ=255)
                             gggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGt                                                               <  2:1358746/100‑1 (MQ=255)
                             gggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGt                                                               <  1:1089099/100‑1 (MQ=255)
                              ggCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTc                                                              >  2:1500388/1‑100 (MQ=255)
                              ggCAAAATTACCGGAGTTGCAGGAAACCATACCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTc                                                              >  1:1011423/1‑100 (MQ=255)
                               gCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCg                                                             >  2:960580/1‑100 (MQ=255)
                               gCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCg                                                             <  1:2194949/100‑1 (MQ=255)
                                cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa                                                            >  1:2464321/1‑100 (MQ=255)
                                cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa                                                            <  2:157655/100‑1 (MQ=255)
                                cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa                                                            >  2:215364/1‑100 (MQ=255)
                                  aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                          <  2:169826/100‑1 (MQ=255)
                                  aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                          <  1:889008/100‑1 (MQ=255)
                                  aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                          <  2:1533038/100‑1 (MQ=255)
                                  aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                          >  1:2297119/1‑100 (MQ=255)
                                   aaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCa                                                         >  2:1973733/1‑100 (MQ=255)
                                   aaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCa                                                         >  1:803210/1‑100 (MQ=255)
                                    aTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCa                                                         >  1:488437/1‑99 (MQ=255)
                                      tACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAAc                                                      >  2:160291/1‑100 (MQ=255)
                                        ccGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACg                                                     >  2:157880/1‑99 (MQ=255)
                                        ccGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGc                                                    >  1:401403/1‑100 (MQ=255)
                                          ggAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTg                                                  <  1:225717/100‑1 (MQ=255)
                                          ggAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTg                                                  >  2:192853/1‑100 (MQ=255)
                                          ggAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTg                                                  >  1:2681109/1‑100 (MQ=255)
                                           gAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGa                                                 <  1:132377/100‑1 (MQ=255)
                                             gTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                               >  1:1479825/1‑100 (MQ=255)
                                               tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                             >  1:2719430/1‑100 (MQ=255)
                                               tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                             >  2:667622/1‑100 (MQ=255)
                                               tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                             >  2:1742567/1‑100 (MQ=255)
                                                 cAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtct                                           >  2:1245594/1‑100 (MQ=255)
                                                   ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                         >  2:150115/1‑100 (MQ=255)
                                                   ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                         >  2:444673/1‑100 (MQ=255)
                                                   ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                         <  1:1920226/100‑1 (MQ=255)
                                                     aaaCCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGt                                       <  2:1479847/100‑1 (MQ=255)
                                                      aaCCAGTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTg                                      <  1:3386272/100‑1 (MQ=255)
                                                       aCCATTCCGGCGGCGATTGATCTGCAGCTTGCCCAGGATCGTACCCCCCCCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTgg                                     <  1:3252422/100‑1 (MQ=255)
                                                        ccATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTgg                                     >  1:1882044/1‑99 (MQ=255)
                                                        ccATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgc                                    <  2:3156370/100‑1 (MQ=255)
                                                         cATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCACACCATTCGCGACTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcg                                   >  1:145105/1‑100 (MQ=255)
                                                          aTTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcgc                                  >  2:2620313/1‑100 (MQ=255)
                                                           ttCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcg                                   >  1:2811567/1‑98 (MQ=255)
                                                             ccGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTgg                               >  2:1409229/1‑100 (MQ=255)
                                                             ccGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTgg                               >  1:1739195/1‑100 (MQ=255)
                                                              cggcggCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                              >  2:2093338/1‑100 (MQ=255)
                                                              cggcggCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                              <  1:188859/100‑1 (MQ=255)
                                                              ccgatctgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                              <  1:3033483/93‑1 (MQ=255)
                                                                 cggctATTGATCTGCAAATTGCCCAGCATCGGTTCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAGTATCTCGGTGGCGCTgg                               <  1:626706/96‑1 (MQ=255)
                                                                 cggcgATTGATCTGCAAATTGCCCAGGCTCGTTCCCCCACCATTCGCGCCTCGTTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGAt                           <  1:1925414/100‑1 (MQ=255)
                                                                 cggcgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGAt                           <  2:3361729/100‑1 (MQ=255)
                                                                 cggcgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCCGGt                              >  1:1659018/1‑97 (MQ=255)
                                                                    cgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt                        >  2:787649/1‑100 (MQ=255)
                                                                    cgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt                        >  1:3226494/1‑100 (MQ=255)
                                                                      aTTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtgg                      <  2:2756522/100‑1 (MQ=255)
                                                                       ttggtatGCAAAGTGCCCAAGATCGGTGCCCCTCCATTTGCGCCTCGCCGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtgg                      <  1:3041127/93‑1 (MQ=255)
                                                                       ttGATCTGTAAATTGCCCAGGTTCGTGCCCCCACCATTCGTGCCTCGGTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggt                     <  1:2200993/100‑1 (MQ=255)
                                                                        tGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtg                    <  2:2329970/100‑1 (MQ=255)
                                                                         gATCTGCAAATTGCCCAGGATCGTTCCCCCTCCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtgg                   <  1:871462/100‑1 (MQ=255)
                                                                         gATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtgg                   >  1:3104795/1‑100 (MQ=255)
                                                                          aTCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTggtggt                  >  2:2299508/1‑100 (MQ=255)
                                                                            cTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGgtgt                <  2:1851916/100‑1 (MQ=255)
                                                                            cTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGgtgt                >  2:883842/1‑100 (MQ=255)
                                                                               cAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtttt             <  2:2297151/100‑1 (MQ=255)
                                                                               cAAATTGCCCAGGATCGTTCCCCCACCATGCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtttt             <  2:1882071/100‑1 (MQ=255)
                                                                                aaaTTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttttt            >  2:3039403/1‑100 (MQ=255)
                                                                                aaaTTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttttt            <  2:2520431/100‑1 (MQ=255)
                                                                                   ttGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCGGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTAtt         <  1:401059/100‑1 (MQ=255)
                                                                                     gCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTcc       >  2:2747814/1‑100 (MQ=255)
                                                                                      nccAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCt      >  2:2683792/2‑100 (MQ=255)
                                                                                       ccAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg     >  2:607816/1‑100 (MQ=255)
                                                                                       ccAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg     <  2:2493981/100‑1 (MQ=255)
                                                                                       ccAGGATCGTCCCCCCACCTTTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTcc       <  1:599806/98‑1 (MQ=255)
                                                                                        cAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg     <  1:2851271/99‑1 (MQ=255)
                                                                                          ggATGGTTCCGCCACCCTTCGCGCCGCGGTGGAAGAAGTCGAGCGAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg     <  1:1112085/97‑1 (MQ=255)
                                                                                          ggATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTgccc  <  2:2347094/100‑3 (MQ=255)
                                                                                                |                                                                                             
CAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGC  >  NC_000913/2159258‑2159447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑