Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,161,946 A→G A161A (GCA→GCG iceT → putative citrate/iron‑citrate/zinc‑citrate efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,161,9460AG98.4% 426.6 / ‑6.6 123A161A (GCA→GCGiceTputative citrate/iron‑citrate/zinc‑citrate efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (58/63);  total (59/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

GCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCG  >  NC_000913/2161849‑2162041
                                                                                                 |                                                                                               
gcgAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTc                                                                                               <  1:3079165/100‑1 (MQ=255)
  gggCAATATATGGCGGCGAGGACCGTTGTCACGTGCGCCGGTCAGGTCGGGCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTTCGcgtcgt                                                                                             <  1:498540/98‑1 (MQ=255)
  gAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGcgtcgt                                                                                             >  1:3281915/1‑100 (MQ=255)
   aGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTg                                                                                            >  2:718354/1‑100 (MQ=255)
   aGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTg                                                                                            <  1:3207536/100‑1 (MQ=255)
     cAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGc                                                                                          <  2:3028983/100‑1 (MQ=255)
     cAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGc                                                                                          >  1:1669021/1‑100 (MQ=255)
     cAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGc                                                                                          <  1:1301096/100‑1 (MQ=255)
        tagatGGCGGCGATGCCCTTTGTCACGTTGCCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACt                                                                                       <  2:3119411/97‑1 (MQ=255)
          tatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTgg                                                                                     <  2:1814549/100‑1 (MQ=255)
           atGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGa                                                                                    >  1:843801/1‑100 (MQ=255)
            tGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGAt                                                                                   <  1:2694990/100‑1 (MQ=255)
             ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATc                                                                                  >  2:2747682/1‑100 (MQ=255)
                   gATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTg                                                                            <  1:2928391/100‑1 (MQ=255)
                   gATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTg                                                                            <  1:2415899/100‑1 (MQ=255)
                   gATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTg                                                                            <  2:27463/100‑1 (MQ=255)
                   gATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTg                                                                            <  2:843812/100‑1 (MQ=255)
                    aTGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGa                                                                           <  1:2005971/100‑1 (MQ=255)
                      gACCCTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATc                                                                         <  2:1408670/100‑1 (MQ=255)
                       aCCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCa                                                                        <  1:1767584/100‑1 (MQ=255)
                       aCCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCa                                                                        <  1:3328386/100‑1 (MQ=255)
                        ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                       >  2:2813647/1‑100 (MQ=255)
                         cTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTTATCAAc                                                                      <  1:708537/100‑1 (MQ=255)
                         cTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAAc                                                                      >  2:869000/1‑100 (MQ=255)
                         cTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAAc                                                                      >  2:1099198/1‑100 (MQ=255)
                          tttGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACa                                                                     >  1:1519958/1‑100 (MQ=255)
                           ttGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACAt                                                                    <  1:3154669/100‑1 (MQ=255)
                           ttGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACAt                                                                    >  2:1221064/1‑100 (MQ=255)
                            tGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACAtt                                                                   >  2:735472/1‑100 (MQ=255)
                              tCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTcc                                                                 >  2:2944464/1‑100 (MQ=255)
                               cACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCg                                                                >  2:1968742/1‑100 (MQ=255)
                               cACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTGTTGAGCAACATTCCg                                                                >  1:3396829/1‑100 (MQ=255)
                                tcGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCgg                                                               <  1:354528/99‑1 (MQ=255)
                                aCGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCgg                                                               >  1:1026194/1‑100 (MQ=255)
                                 cGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGt                                                              >  1:1486441/1‑100 (MQ=255)
                                  gTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTg                                                             <  2:2307202/100‑1 (MQ=255)
                                    tACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTggg                                                           <  2:48478/100‑1 (MQ=255)
                                     aCCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTgggg                                                          >  1:1835091/1‑100 (MQ=255)
                                      cccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGa                                                         >  2:2582097/1‑100 (MQ=255)
                                       ccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGAt                                                        <  1:2637237/100‑1 (MQ=255)
                                       ccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGAt                                                        <  2:2484991/100‑1 (MQ=255)
                                        cGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTggg                                                           <  1:1365852/96‑1 (MQ=255)
                                        cGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTGTTTGATCAACATTCCGGTGGGGAtt                                                       >  1:2217801/1‑100 (MQ=255)
                                         ggTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTa                                                      <  2:483364/100‑1 (MQ=255)
                                         ggTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTa                                                      >  2:2270229/1‑100 (MQ=255)
                                           tCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTTGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATc                                                    <  1:1481131/100‑1 (MQ=255)
                                            cAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCg                                                   <  1:2813603/100‑1 (MQ=255)
                                              ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGTTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                 >  2:2518725/1‑100 (MQ=255)
                                              ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                 >  1:1665710/1‑100 (MQ=255)
                                              ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                 >  1:749844/1‑100 (MQ=255)
                                               gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                <  2:1843803/100‑1 (MQ=255)
                                                   gtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGAt                                            >  2:2562777/1‑100 (MQ=255)
                                                   gtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGAt                                            >  1:452344/1‑100 (MQ=255)
                                                    tcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATc                                           >  1:801323/1‑100 (MQ=255)
                                                      cGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTTGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCg                                          <  2:946212/99‑1 (MQ=255)
                                                       gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTTCGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGcc                                        <  1:1897037/100‑1 (MQ=255)
                                                       gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGcc                                        <  2:2571003/100‑1 (MQ=255)
                                                          gctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCcaca                                     <  1:732883/100‑1 (MQ=255)
                                                          gctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCcaca                                     >  1:2814370/1‑100 (MQ=255)
                                                          gctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCcaca                                     >  2:169029/1‑100 (MQ=255)
                                                             cGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACAt                                    >  1:684247/1‑98 (MQ=255)
                                                              ggTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGc                                 <  1:1666785/100‑1 (MQ=255)
                                                                tCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTg                               <  2:1026207/100‑1 (MQ=255)
                                                                tCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTg                               <  2:248364/100‑1 (MQ=255)
                                                                 ccGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTg                               <  1:1367827/99‑1 (MQ=255)
                                                                 ccGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGt                              >  2:1241644/1‑100 (MQ=255)
                                                                 ccGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGt                              >  1:1876132/1‑100 (MQ=255)
                                                                  cGGCGCTCGGCGGTCTGTTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTTCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGtt                             <  1:1495984/100‑1 (MQ=255)
                                                                  cGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGtt                             <  2:1650724/100‑1 (MQ=255)
                                                                      gcTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATg                         <  1:1259005/100‑1 (MQ=255)
                                                                      gcTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGAGCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAAt                          <  1:48467/99‑1 (MQ=255)
                                                                         cggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGTTCGCCACATTGCTGTTAATGcc                       <  1:2017734/99‑1 (MQ=255)
                                                                         cggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCg                      <  1:1974460/100‑1 (MQ=255)
                                                                          ggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGa                     <  2:1427428/100‑1 (MQ=255)
                                                                           ggggTCTGCTGGTAGAGTACGCGTCGTGGCAGTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGaa                    >  2:2339483/3‑100 (MQ=255)
                                                                           gcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGaa                    <  1:1349406/100‑1 (MQ=255)
                                                                            cggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                   >  1:1810112/1‑100 (MQ=255)
                                                                            cggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                   <  2:2293291/100‑1 (MQ=255)
                                                                             ggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACt                  >  2:899138/1‑100 (MQ=255)
                                                                              gTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTa                 >  2:935927/1‑100 (MQ=255)
                                                                               tCTGCTGGTGGAGTACGCGTCGTGGCCCTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAGTac                >  1:2648450/1‑100 (MQ=255)
                                                                                ctgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTaca               <  2:2814414/100‑1 (MQ=255)
                                                                                ctgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTaca               <  2:362410/100‑1 (MQ=255)
                                                                                ctgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTaca               >  2:2291463/1‑100 (MQ=255)
                                                                                 tgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTacac              >  1:3370615/1‑100 (MQ=255)
                                                                                 tgctgGTGGAGTACGCGTCGTGGCACTGGATCTATTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTacac              >  1:2261946/1‑100 (MQ=255)
                                                                                   ctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACAcc             >  1:3128334/1‑99 (MQ=255)
                                                                                   ctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCa            <  2:3246202/100‑1 (MQ=255)
                                                                                   ctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCa            >  2:2626393/1‑100 (MQ=255)
                                                                                   ctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCa            >  1:1623797/1‑100 (MQ=255)
                                                                                   ctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCa            >  1:470227/1‑100 (MQ=255)
                                                                                    tggtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCAt           <  1:2265247/100‑1 (MQ=255)
                                                                                     ggtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATg          >  2:15333/1‑100 (MQ=255)
                                                                                     ggtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATg          >  1:2718320/1‑100 (MQ=255)
                                                                                      gtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGc         <  1:1222077/100‑1 (MQ=255)
                                                                                      gtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGc         <  1:455095/100‑1 (MQ=255)
                                                                                      gtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGc         <  1:1132879/100‑1 (MQ=255)
                                                                                        ggAGTACGCGTCGTGGCACTGGATCTTTTTTTTTTTTATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCAttc         >  1:2772993/1‑96 (MQ=255)
                                                                                         gAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGa      <  2:484603/100‑1 (MQ=255)
                                                                                         gAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGa      <  2:753735/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCG  >  NC_000913/2161849‑2162041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: