Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,076 C→A pseudogene (287/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,0760CA97.5% 408.5 / ‑0.1 122pseudogene (287/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (51/68);  total (52/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 122 total aligned reads displayed.

TGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCC  >  NC_000913/2172980‑2173171
                                                                                                |                                                                                               
tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGcc                                                                                              >  1:1205054/1‑100 (MQ=255)
 gAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGccc                                                                                             <  1:2729366/100‑1 (MQ=255)
  aaaaTACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCt                                                                                            >  2:722976/1‑100 (MQ=255)
      tACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATc                                                                                        <  2:281657/100‑1 (MQ=255)
        ccGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCa                                                                                      <  1:546383/100‑1 (MQ=255)
         cGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAt                                                                                     >  2:168425/1‑100 (MQ=255)
         cGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAt                                                                                     >  1:1342674/1‑100 (MQ=255)
          ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAtt                                                                                    <  1:1127715/100‑1 (MQ=255)
          ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCAtt                                                                                    <  2:3144786/100‑1 (MQ=255)
          ggTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATAATGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGGACAGCCCTGATCCAtt                                                                                    >  1:2192730/1‑100 (MQ=255)
           gTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTg                                                                                   <  1:2744253/100‑1 (MQ=255)
           gTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTg                                                                                   <  2:1803476/100‑1 (MQ=255)
           gTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAACCCTGATCCATTg                                                                                   <  1:2245992/100‑1 (MQ=255)
              aGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAg                                                                                <  1:1842512/100‑1 (MQ=255)
               gATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGc                                                                               >  1:3370556/1‑100 (MQ=255)
               gATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGc                                                                               >  1:95511/1‑100 (MQ=255)
               gATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGc                                                                               >  2:2206389/1‑100 (MQ=255)
                aTAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGcc                                                                              <  2:1772108/100‑1 (MQ=255)
                   aaTTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGccacc                                                                           >  1:1729863/1‑100 (MQ=255)
                   aaTTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGccacc                                                                           <  2:2396411/100‑1 (MQ=255)
                   aaTTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGccacc                                                                           <  1:2657353/100‑1 (MQ=255)
                    aTTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGccacca                                                                          <  2:590296/100‑1 (MQ=255)
                     ttGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCAt                                                                         <  1:2557527/100‑1 (MQ=255)
                     ttGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCCGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCAt                                                                         <  2:902122/100‑1 (MQ=255)
                      tGCACCGATAATAATGAAACCGGGAATCTTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGCTCCATTGAAGCCACCATa                                                                        <  2:868218/100‑1 (MQ=255)
                      tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATa                                                                        >  1:662175/1‑100 (MQ=255)
                      tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATa                                                                        >  2:390956/1‑100 (MQ=255)
                      tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATa                                                                        <  1:1277420/100‑1 (MQ=255)
                       gCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAc                                                                       >  2:1035268/1‑100 (MQ=255)
                       gCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAc                                                                       <  2:1886271/100‑1 (MQ=255)
                        cACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAcc                                                                      >  2:417789/1‑100 (MQ=255)
                        cACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAcc                                                                      <  1:1594960/100‑1 (MQ=255)
                        cACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAcc                                                                      <  1:2870474/100‑1 (MQ=255)
                          ccGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATCGGAGAGCAGCCCTGATCCATTGAAGCCACCATAcccc                                                                    >  1:2340899/1‑100 (MQ=255)
                           cGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATAccccc                                                                   >  2:2719418/1‑100 (MQ=255)
                             ataataATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCgg                                                                 <  2:1501881/100‑1 (MQ=255)
                              taataaTGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGc                                                                <  2:1264304/100‑1 (MQ=255)
                               aataatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCt                                                               <  2:2992189/100‑1 (MQ=255)
                                  aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCtttt                                                            <  1:1474015/100‑1 (MQ=255)
                                  aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCtttt                                                            <  2:1205069/100‑1 (MQ=255)
                                  aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCtttt                                                            <  1:114916/100‑1 (MQ=255)
                                  aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCtttt                                                            >  1:1304072/1‑100 (MQ=255)
                                   atGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTa                                                           >  2:2493178/1‑100 (MQ=255)
                                   atGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTa                                                           <  1:3457879/100‑1 (MQ=255)
                                   atGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTa                                                           >  2:3434606/1‑100 (MQ=255)
                                   aggAAACCGGGAATATTTTGCGGGGGGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTa                                                           <  1:2808934/98‑1 (MQ=255)
                                    tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCttttta                                                          <  2:1259565/100‑3 (MQ=255)
                                    tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTCaa                                                          >  1:1039554/1‑100 (MQ=255)
                                      aaaCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCTCCCATACCTCCGGCTTCAAACg                                                        >  1:2886173/1‑100 (MQ=255)
                                      aaaCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACg                                                        >  2:2699893/1‑100 (MQ=255)
                                      aaaCCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACg                                                        <  1:913291/100‑1 (MQ=255)
                                         ccGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGcc                                                      <  1:1700300/99‑1 (MQ=255)
                                            ggAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAg                                                  >  1:2926863/1‑100 (MQ=255)
                                              aaTATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCa                                                <  1:2206357/100‑1 (MQ=255)
                                                tatTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCAtt                                              <  1:2589003/100‑1 (MQ=255)
                                                tatTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCAtt                                              <  1:186380/100‑1 (MQ=255)
                                                   tttGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgc                                           >  1:421645/1‑100 (MQ=255)
                                                     tGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgccg                                         <  1:2984714/100‑1 (MQ=255)
                                                      gCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgccgc                                        <  1:248527/100‑1 (MQ=255)
                                                       cGGGGAGAAAACCTGAGTCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAgccgcc                                       <  1:1273848/100‑1 (MQ=255)
                                                        ggggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCa                                      <  2:844339/100‑1 (MQ=255)
                                                         gggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAg                                     >  1:1269924/1‑100 (MQ=255)
                                                         gggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAg                                     <  2:1304091/100‑1 (MQ=255)
                                                          ggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGt                                    >  1:733498/1‑100 (MQ=255)
                                                            agaAAACCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTg                                  <  2:3278437/100‑1 (MQ=255)
                                                              aaaaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTggg                                <  1:3340441/100‑1 (MQ=255)
                                                              aaaaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTggg                                <  2:1273769/100‑1 (MQ=255)
                                                               aaaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGgt                               >  1:675761/1‑100 (MQ=255)
                                                                aaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGgtg                              <  2:1729887/100‑1 (MQ=255)
                                                                aaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGgtg                              <  2:2261592/100‑1 (MQ=255)
                                                                aaCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGgt                               >  2:741070/1‑99 (MQ=255)
                                                                 aCCTGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGgtgt                             >  2:1376406/1‑100 (MQ=255)
                                                                    tGAATCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCa                          <  2:3370608/100‑1 (MQ=255)
                                                                       aTCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAc                       >  2:233740/1‑100 (MQ=255)
                                                                       aTCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAc                       <  1:1995508/100‑1 (MQ=255)
                                                                        tCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAcc                      <  2:2926910/100‑1 (MQ=255)
                                                                        tCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAcc                      >  1:2854818/1‑100 (MQ=255)
                                                                        tCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAcc                      <  2:2779462/100‑1 (MQ=255)
                                                                        tCAGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAcc                      <  1:1684529/100‑1 (MQ=255)
                                                                          aGTAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCaa                    >  2:1270376/1‑100 (MQ=255)
                                                                            tAACCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATa                  >  2:2663523/1‑100 (MQ=255)
                                                                             aaCCAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAg                 <  2:95512/100‑1 (MQ=255)
                                                                               ccAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAActattggtt               <  1:2043084/100‑9 (MQ=255)
                                                                                cAGGTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGttt              >  2:1808285/1‑100 (MQ=255)
                                                                                  ggTAATGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGc            <  2:607907/100‑1 (MQ=255)
                                                                                     aaTGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTc         >  1:1419020/1‑100 (MQ=255)
                                                                                       tGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAgatcggaagag       >  1:1400035/1‑90 (MQ=255)
                                                                                       tGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc       >  2:861106/1‑100 (MQ=255)
                                                                                       tGGGAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTcgc       <  1:1616838/100‑1 (MQ=255)
                                                                                        gggAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACTATTTGTTTGcacaccc      <  2:2288123/100‑7 (MQ=255)
                                                                                         ggAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGa     >  2:2984500/1‑100 (MQ=255)
                                                                                         ggAGAACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGa     <  2:1817845/100‑1 (MQ=255)
                                                                                          gagaACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGAt    >  2:405062/1‑100 (MQ=255)
                                                                                           agaACAGCCCTGATCCATTGAAGCCACCATACCCACGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATc   <  2:2851892/100‑1 (MQ=255)
                                                                                            gaACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGTTcc  <  1:104922/100‑1 (MQ=255)
                                                                                            gaACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc  >  1:1623484/1‑100 (MQ=255)
                                                                                            gaACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc  <  1:1446931/100‑1 (MQ=255)
                                                                                            gaACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc  <  1:2961737/100‑1 (MQ=255)
                                                                                            gaACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc  >  1:878231/1‑100 (MQ=255)
                                                                                            gaACAGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATcc  <  2:111465/100‑1 (MQ=255)
                                                                                                |                                                                                               
TGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCC  >  NC_000913/2172980‑2173171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: