Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,927,735 Δ1 bp 50.4% coding (581/993 nt) asnA → asparagine synthetase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,927,7340G.50.4% 206.8 / inf 129coding (580/993 nt)asnAasparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base . (31/34);  total (63/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

CTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGC  >  NC_000913/3927635‑3927833
                                                                                                   |                                                                                                   
cTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTg                                                                                                     >  2:2662063/1‑100 (MQ=255)
 tGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgg                                                                                                    >  1:2549803/1‑100 (MQ=255)
    cGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGgcgc                                                                                                 >  2:3270056/1‑100 (MQ=255)
     ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGcgcg                                                                                                >  2:521524/1‑100 (MQ=255)
     ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGcgcg                                                                                                >  2:3178315/1‑100 (MQ=255)
     ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGACAGCGAAAGATCTTGGcgcg                                                                                                <  2:966450/100‑1 (MQ=255)
        tCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTa                                                                                             <  1:337470/100‑1 (MQ=255)
        tCAGATCCACTTCGGACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTAt                                                                                            <  1:1038619/100‑1 (MQ=255)
         cAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTAtt                                                                                           >  1:950141/1‑100 (MQ=255)
          aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTAtt                                                                                           >  2:3057123/1‑100 (MQ=255)
          aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTc                                                                                          >  2:811173/1‑100 (MQ=255)
           gATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTcc                                                                                         >  1:251702/1‑100 (MQ=255)
            aTCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTcc                                                                                         <  2:2669223/100‑1 (MQ=255)
            aTCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCt                                                                                        >  1:1992647/1‑100 (MQ=255)
            aTCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCt                                                                                        <  2:415795/100‑1 (MQ=255)
             tCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCtt                                                                                       >  2:648273/1‑100 (MQ=255)
              ccACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCt                                                                                        <  2:142649/99‑1 (MQ=255)
              ccACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTg                                                                                      <  1:263829/100‑1 (MQ=255)
               cACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTg                                                                                      >  1:722660/1‑100 (MQ=255)
               cACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTg                                                                                      >  2:2491929/1‑100 (MQ=255)
               cACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGt                                                                                     <  2:593065/100‑1 (MQ=255)
                aCTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGTCGCGGTATTCCTTGt                                                                                     >  2:3336119/1‑100 (MQ=255)
                 cTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTc                                                                                    <  2:138710/100‑1 (MQ=255)
                  ttCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGTGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                                  <  1:1829357/100‑1 (MQ=255)
                  ttCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                                  >  2:2781169/1‑100 (MQ=255)
                  ttCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                                  <  2:1082171/100‑1 (MQ=255)
                    cGTACACAGCCAGGAGTTGCTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGCTAGCGAAAGATCTTGGCGCGGTATTCCTTGTCg                                                                                   <  1:2240124/98‑1 (MQ=255)
                    cGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGTGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCggg                                                                                 <  2:192794/100‑1 (MQ=255)
                      tACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGAt                                                                               >  2:1012712/1‑100 (MQ=255)
                        cactGCCGGGGGTTACTGTCGCGTGATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTg                                                                             <  1:1457711/100‑1 (MQ=255)
                        cacaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTg                                                                             >  2:236134/1‑100 (MQ=255)
                        cacaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTgg                                                                            <  1:1507404/100‑1 (MQ=255)
                         acaGCCAGGAGTTACTGTCTCGTTATCCGGATTTTGATGCCAAAGGGCGTGAGCGTGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCTGGATTg                                                                             <  1:986156/99‑1 (MQ=255)
                          caggcaggAGTTACTGTCTCGTTAGCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcg                                                                          <  1:2139871/100‑1 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCggagatcgg                                                                           >  1:1193551/1‑93 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggc                                                                           >  2:3240311/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGCAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcgg                                                                         >  2:3210631/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcgg                                                                         <  1:3113023/100‑1 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcgg                                                                         >  1:1467253/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcgg                                                                         >  1:2269017/1‑100 (MQ=255)
                            gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcgg                                                                         >  1:1618319/1‑100 (MQ=255)
                               aGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCaa                                                                      <  1:529852/100‑1 (MQ=255)
                               aGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAg                                                                     >  1:3192359/1‑100 (MQ=255)
                                ggAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAg                                                                     <  2:3053290/100‑1 (MQ=255)
                                 gAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCt                                                                   >  2:449705/1‑100 (MQ=255)
                                  aGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCt                                                                   <  1:1012709/100‑1 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                               <  2:888540/100‑1 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                               <  1:760029/100‑1 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                               >  1:537068/1‑100 (MQ=255)
                                      aCTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                               <  2:2836932/100‑1 (MQ=255)
                                        tGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGa                                                             >  1:302198/1‑100 (MQ=255)
                                         gTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATg                                                           <  2:395896/100‑1 (MQ=255)
                                          tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                          <  2:2504522/100‑1 (MQ=255)
                                           ctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                          <  1:667048/100‑1 (MQ=255)
                                           ctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                          <  1:2662041/100‑1 (MQ=255)
                                           ctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                          <  1:1143317/99‑1 (MQ=255)
                                            tcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGt                                                         >  1:2494566/1‑100 (MQ=255)
                                              gTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCa                                                       <  1:521521/100‑1 (MQ=255)
                                                tATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCAtag                                                    >  1:402513/1‑98 (MQ=255)
                                                   ccGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCa                                                 >  1:1836/1‑100 (MQ=255)
                                                    cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCac                                                >  2:3034795/1‑100 (MQ=255)
                                                    cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCac                                                >  1:1750174/1‑100 (MQ=255)
                                                    cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCa                                                 >  1:1416801/1‑99 (MQ=255)
                                                      gATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCac                                                <  1:1625428/98‑1 (MQ=255)
                                                        tCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgac                                             >  2:889223/1‑100 (MQ=255)
                                                        tCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgacg                                            <  1:2442980/100‑1 (MQ=255)
                                                         cTTGATGCCAAAGGGCGTGAGCGGGCGAGAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgacg                                            <  1:190475/99‑1 (MQ=255)
                                                          ttGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGt                                           >  2:2352838/1‑100 (MQ=255)
                                                          ttGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTg                                          >  2:810268/1‑100 (MQ=255)
                                                          ttGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTg                                          <  2:2615787/100‑1 (MQ=255)
                                                             aTGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgc                                       >  2:1689845/1‑100 (MQ=255)
                                                              tGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgc                                       >  2:1816333/1‑100 (MQ=255)
                                                              tGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgc                                       >  2:1789775/1‑99 (MQ=255)
                                                               gCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgcg                                      >  1:2313376/1‑100 (MQ=255)
                                                               gCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgcg                                      <  2:555612/100‑1 (MQ=255)
                                                               gCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgcgc                                     <  2:1620231/100‑1 (MQ=255)
                                                                 cAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCa                                    >  1:924910/1‑100 (MQ=255)
                                                                 cAAAGGGCGAGAGCGGGCGATTGCGAAAGATCTTCGCGCGGTATTCCTTGTCGGGCTGGGCGGCAAGCTGTGCGATGGTCATCGCCTCGACGTGCGCTCa                                    >  1:209413/1‑100 (MQ=255)
                                                                     gggCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGa                               <  2:1836/100‑1 (MQ=255)
                                                                      ggCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAt                              <  2:2108492/100‑1 (MQ=255)
                                                                         gTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTat                           >  2:2185026/1‑100 (MQ=255)
                                                                          tGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatg                          <  1:2384665/100‑1 (MQ=255)
                                                                            aGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                        <  1:900990/100‑1 (MQ=255)
                                                                              cGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGTGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                        <  2:2549823/99‑1 (MQ=255)
                                                                               gggCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGAGACAAAATAAGAGATGGTCATCGCCACGACGTGCGCGCACCGGATTAtgatga                      <  1:3336091/100‑1 (MQ=255)
                                                                               gggCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGAc                     >  2:222778/1‑100 (MQ=255)
                                                                                 gCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg                   <  2:835732/100‑1 (MQ=255)
                                                                                 gCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg                   >  2:1403998/1‑100 (MQ=255)
                                                                                 gCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg                   <  1:983087/100‑1 (MQ=255)
                                                                                    aTAGCGAAAGATCTTGGCGCGTTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCTCCACGACGTGCGCGCACCGGATTATGATGACTGGa                 <  1:2270627/100‑1 (MQ=255)
                                                                                      aGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGc               <  2:722663/100‑1 (MQ=255)
                                                                                         gAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcc            <  1:1922952/100‑1 (MQ=255)
                                                                                         gAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcc            <  2:76313/100‑1 (MQ=255)
                                                                                         gAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcc            <  1:212993/100‑1 (MQ=255)
                                                                                         gAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAc             >  1:57609/1‑99 (MQ=255)
                                                                                         gAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAccc           >  2:3201638/1‑100 (MQ=255)
                                                                                          aaaGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAccc           <  2:2808393/100‑1 (MQ=255)
                                                                                           aaGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCg         >  2:1105209/1‑100 (MQ=255)
                                                                                             gATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTc       <  2:1629510/100‑1 (MQ=255)
                                                                                                   ggCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGc  >  1:2730894/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGC  >  NC_000913/3927635‑3927833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑