Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,500,814 Δ1 bp 100% intergenic (‑23/‑446) yjgX ← / → yjgZ pseudogene fragment/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,500,8110T.63.0% 91.9 / 170.6 100intergenic (‑20/‑449)yjgX/yjgZpseudogene fragment/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (13/23);  new base . (31/32);  total (44/56)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGG  >  NC_000913/4500713‑4500906
                                                                                                  |                                                                                               
tCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtt                                                                                                <  2:1745502/100‑1 (MQ=255)
 ccGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGttt                                                                                               >  1:3191330/1‑100 (MQ=255)
   gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct                                                                                             >  2:2592876/1‑98 (MQ=255)
   gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTc                                                                                             >  1:2551307/1‑100 (MQ=255)
   gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCACAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTc                                                                                             <  1:1753331/100‑1 (MQ=255)
    ccAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCt                                                                                            >  2:739266/1‑100 (MQ=255)
     cAGAAAATCACCACGAATACCAGAATCGCCGCACTGACCAGAAAATGATAAACAGGAAACGCGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTg                                                                                           <  1:1959775/100‑1 (MQ=255)
       gAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTa                                                                                         >  1:1476500/1‑100 (MQ=255)
       gAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAg                                                                                        <  2:199415/100‑1 (MQ=255)
         aaaTCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGt                                                                                      <  1:2373610/100‑1 (MQ=255)
           aTCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGt                                                                                      >  1:2122266/1‑99 (MQ=255)
            tCACCACGAATACCAGAAGCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCt                                                                                   <  2:2800020/100‑1 (MQ=255)
             caccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTc                                                                                    <  1:774885/98‑1 (MQ=255)
             caccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTg                                                                                  <  2:717795/100‑1 (MQ=255)
              accacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGa                                                                                 >  2:3084518/1‑100 (MQ=255)
                cacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAc                                                                               <  2:516581/100‑1 (MQ=255)
                 acGAATACCAGAATCGCGGCACTGACCCGAAAATGCTAAACAGGACACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAAc                                                                               <  2:3177835/100‑1 (MQ=255)
                 acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAAc                                                                               <  2:3072187/100‑1 (MQ=255)
                 acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGCTCTGAAc                                                                               <  2:1773521/100‑1 (MQ=255)
                 acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAcc                                                                              >  2:2054216/1‑100 (MQ=255)
                   gAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCgg                                                                            <  2:2492375/100‑1 (MQ=255)
                   gAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCgg                                                                            <  2:1692114/100‑1 (MQ=255)
                    aaTACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCgg                                                                            <  2:1061851/100‑1 (MQ=255)
                    aaTACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCATGTAGTGTTTTCTGTAGGTTCTGAACCgg                                                                            <  1:2908480/100‑1 (MQ=255)
                     aTACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGtt                                                                          <  2:3188731/100‑1 (MQ=255)
                       aCCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTc                                                                         >  1:1081264/1‑99 (MQ=255)
                        ccAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTc                                                                         <  1:1149828/99‑1 (MQ=255)
                        ccAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTa                                                                       >  2:919094/1‑100 (MQ=255)
                          aGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTa                                                                       >  1:901871/1‑99 (MQ=255)
                                gcgGCACTGACCAGAAAATGATAAACAGGAATCACGGTGCGGTTCTCTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGtt                                                               <  2:1521946/100‑1 (MQ=255)
                                gcgGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGAGTTTTCTGTAGGTTCTGAACCGGTTCTAGCgggg                                                                 >  1:3162030/1‑99 (MQ=255)
                                   gCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGATTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtgtg                                                            >  1:1384206/1‑100 (MQ=255)
                                    cACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtgtg                                                            <  2:2124339/100‑1 (MQ=255)
                                    cACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGa                                                           <  2:28866/100‑1 (MQ=255)
                                     aCTGACCAGAAAATGATAAACAGGGAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGCTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGa                                                           <  1:1495514/100‑1 (MQ=255)
                                     aCTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAg                                                          <  2:704864/100‑1 (MQ=255)
                                      cTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAg                                                          >  2:1975202/1‑100 (MQ=255)
                                      cTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAgg                                                         >  1:250706/1‑100 (MQ=255)
                                      cTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAg                                                          <  1:338818/99‑1 (MQ=255)
                                        gACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGc                                                        <  1:739263/100‑1 (MQ=255)
                                        gACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCt                                                       >  1:839901/1‑100 (MQ=255)
                                          ccAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTg                                                      <  2:2551327/100‑1 (MQ=255)
                                          ccAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGa                                                     >  2:2193189/1‑100 (MQ=255)
                                          ccAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGa                                                     >  2:2647319/1‑100 (MQ=255)
                                          ccAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCGTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGGTGTGAGGCTGa                                                     >  2:66814/1‑100 (MQ=255)
                                          ccAGAAAATGATAAACAGGAAACACGGGGCGATTCACTCTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCt                                                       <  1:2375734/99‑1 (MQ=255)
                                            aGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATg                                                   >  1:1157266/1‑100 (MQ=255)
                                             gAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg                                                  <  1:2704448/100‑1 (MQ=255)
                                             gAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg                                                  <  2:47785/100‑1 (MQ=255)
                                              aaaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg                                                  <  1:3317622/100‑1 (MQ=255)
                                              aaaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGc                                                 <  1:1098332/100‑1 (MQ=255)
                                               aaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGc                                                 <  2:1890696/100‑1 (MQ=255)
                                               aaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCa                                                >  2:2238780/1‑100 (MQ=255)
                                                 aTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCaa                                               <  2:1152445/100‑1 (MQ=255)
                                                 aTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAAt                                              >  2:626466/1‑100 (MQ=255)
                                                   gATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATc                                             <  2:2203425/100‑1 (MQ=255)
                                                    aTAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGc                                           >  1:2785096/1‑100 (MQ=255)
                                                     tAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGcc                                          >  2:1708349/1‑100 (MQ=255)
                                                        aCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCgg                                        <  2:429664/100‑1 (MQ=255)
                                                        aCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCggg                                       >  2:2333574/1‑100 (MQ=255)
                                                        aCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCggg                                       >  2:3111064/1‑100 (MQ=255)
                                                         cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCggg                                       >  1:952100/1‑100 (MQ=255)
                                                           ggAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGctc                                    >  1:1514667/1‑100 (MQ=255)
                                                            gAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGctct                                   >  2:2337414/1‑100 (MQ=255)
                                                               acacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc                                <  2:43819/100‑1 (MQ=255)
                                                               acacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc                                <  2:2475627/100‑1 (MQ=255)
                                                                cacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc                                >  2:2912020/1‑100 (MQ=255)
                                                                cacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTcc                                <  2:2742662/100‑1 (MQ=255)
                                                                cacGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGTGGCTGATGGCAATCGCCGGGCTCTTCCt                               >  1:3154728/1‑100 (MQ=255)
                                                                  cGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGc                             <  2:678270/100‑1 (MQ=255)
                                                                   tccgatcttCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCa                            <  2:2670604/93‑1 (MQ=255)
                                                                   ggggCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCa                            <  1:1438277/100‑1 (MQ=255)
                                                                   ggggCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCa                            <  2:2813250/100‑1 (MQ=255)
                                                                     ggCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATg                          <  2:1193365/100‑1 (MQ=255)
                                                                       cGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGt                         >  1:2829/1‑100 (MQ=255)
                                                                        ggTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGtt                        >  2:2790204/1‑100 (MQ=255)
                                                                        ggTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGtt                        <  2:414887/100‑1 (MQ=255)
                                                                         gTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTac                      >  2:2583530/1‑100 (MQ=255)
                                                                           tCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCaagatcgga                    >  1:2670582/1‑92 (MQ=255)
                                                                           tCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTacac                    >  1:1362076/1‑100 (MQ=255)
                                                                           tCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTacac                    >  1:1360940/1‑100 (MQ=255)
                                                                             agtttGCTTTCTCCTGGAGTGTTTTCTGTAGGTTCTCAACCGGTTCTAGCGAAGTTGTGAGGCTGATGGCAATCGCCGGGCACTTCCTGCAGGTTacaca                   <  1:1832430/98‑2 (MQ=255)
                                                                             aCTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACt                   <  2:1231139/100‑1 (MQ=255)
                                                                             aCTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTg                  <  2:1293226/100‑1 (MQ=255)
                                                                              cTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 >  2:173387/1‑100 (MQ=255)
                                                                              cTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 >  2:2006531/1‑100 (MQ=255)
                                                                              cTTTGCTTTCTCCTGAAGTGTTTTCTGTAGGTTCTGATCCGGCTCTAGCGGGGTTGTTAGGCTGATGGCAAACGCCGGGCTCTTCCTGCATGTTACACTg                  >  1:2292422/1‑100 (MQ=255)
                                                                               tttGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 >  2:1410477/1‑100 (MQ=255)
                                                                                  gCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACt             >  2:2097138/1‑100 (MQ=255)
                                                                                     ttCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGc           <  2:1476506/100‑1 (MQ=255)
                                                                                     ttCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCa          >  1:2132731/1‑100 (MQ=255)
                                                                                      tctcCTGTAGTGTTTCTGTAGGATCTGAACCGGTTCTTGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCaa         <  1:2285983/100‑1 (MQ=255)
                                                                                       ctcCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAag        <  1:2193174/100‑1 (MQ=255)
                                                                                        tcCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAaga       >  2:3061744/1‑100 (MQ=255)
                                                                                         ccTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAagag      >  2:2760806/1‑100 (MQ=255)
                                                                                          cTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgg     <  2:1117843/100‑1 (MQ=255)
                                                                                           tGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggg    <  2:93914/100‑1 (MQ=255)
                                                                                            gTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGGGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCATGCATGTTACACTGGTACTGCAAGAgggg   >  1:2031691/1‑100 (MQ=255)
                                                                                            gTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCACGAgggg   <  1:919091/100‑1 (MQ=255)
                                                                                              aGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggggg  <  2:1645260/100‑1 (MQ=255)
                                                                                                  |                                                                                               
TCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGG  >  NC_000913/4500713‑4500906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: