Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,170,338 (A)6→5 100% coding (37/963 nt) ldtC ← L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,170,3330A.56.5% 24.9 / 224.1 108coding (42/963 nt)ldtCL,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base . (23/38);  total (47/61)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 116 total aligned reads displayed.

ATGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGG  >  NC_000913/1170234‑1170431
                                                                                                   |                                                                                                  
aTGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTa                                                                                                    <  2:1295969/100‑1 (MQ=255)
  gAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaa                                                                                                  <  1:1363749/100‑1 (MQ=255)
  gAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaa                                                                                                  >  1:2389396/1‑100 (MQ=255)
  gAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaa                                                                                                  >  2:2343666/1‑100 (MQ=255)
   aaaTTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaa                                                                                                 >  2:2918906/1‑100 (MQ=255)
       ttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacgt                                                                                             <  1:1800545/100‑4 (MQ=255)
       ttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACg                                                                                             <  2:3363763/100‑1 (MQ=255)
       ttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACg                                                                                             >  1:2884165/1‑100 (MQ=255)
        tGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacgtt                                                                                            <  1:1171762/100‑5 (MQ=255)
         gTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGtt                                                                                           >  2:1610801/1‑100 (MQ=255)
          ttttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTa                                                                                          <  1:2680770/100‑1 (MQ=255)
            ttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAGAACGTTAGcc                                                                                       <  2:689896/100‑1 (MQ=255)
            ntCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGc                                                                                        >  1:309674/2‑100 (MQ=255)
             tCGCCAACCAGACGACGGCCCGCTGGCGGCATCGGCCAGGTGTGGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGc                                                                                        <  1:377678/99‑1 (MQ=255)
                ccAACCAGACGACTGCCCACTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGc                                                                                    <  1:3250968/100‑1 (MQ=255)
                 cAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCga                                                                                  >  1:2886545/1‑100 (MQ=255)
                  aaCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCga                                                                                  >  1:1923971/1‑100 (MQ=255)
                  aaCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCgag                                                                                 >  1:1692413/1‑100 (MQ=255)
                  aaCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCgag                                                                                 >  1:508384/1‑100 (MQ=255)
                     cAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGaaa                                                                              <  1:1313582/100‑1 (MQ=255)
                      aGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGaaaa                                                                             <  1:1555682/100‑1 (MQ=255)
                       gacgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGaaaa                                                                             >  1:1744842/1‑100 (MQ=255)
                         cgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCATGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAAc                                                                           >  1:813650/1‑100 (MQ=255)
                           ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAAcgc                                                                         >  1:2043316/2‑100 (MQ=255)
                           ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGAGCCACGGCAGCGGGGAACGTAAAAACGTTAGTCAGCGTGAAAAAcgcg                                                                        >  1:2231017/2‑100 (MQ=255)
                           acTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                        <  2:2343996/100‑1 (MQ=255)
                            cTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                        >  2:2168208/1‑100 (MQ=255)
                             tggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGCACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGt                                                                       >  2:2607009/4‑100 (MQ=255)
                              gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGtt                                                                      >  2:1164231/1‑100 (MQ=255)
                              gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGtt                                                                      >  2:2083092/1‑100 (MQ=255)
                              gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgc                                                                         >  1:3258752/1‑96 (MQ=255)
                               cccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGtttt                                                                    <  2:1975590/100‑1 (MQ=255)
                                  gCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGat                                                                 <  2:2325270/100‑1 (MQ=255)
                                   cTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGat                                                                 <  1:2441386/100‑1 (MQ=255)
                                   cTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGat                                                                 <  2:1939808/100‑1 (MQ=255)
                                     ggcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatca                                                               <  1:646184/100‑1 (MQ=255)
                                     ggcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcat                                                              >  1:3177736/1‑100 (MQ=255)
                                      gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc                                                             <  2:551956/100‑1 (MQ=255)
                                      gcggcAGCGGCCAGGTGTTGGCATTTGACGATAGCTCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatc                                                                <  1:3206330/98‑1 (MQ=255)
                                         gcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCAc                                                           >  1:2664030/1‑100 (MQ=255)
                                          cAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAATGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCAcc                                                          <  2:2389413/100‑1 (MQ=255)
                                           aGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaa                                                        >  1:3250820/1‑100 (MQ=255)
                                           aGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTAAAAAAAAAAAAAAAACGCGTTTTGATCATCACCa                                                         <  1:1900675/99‑1 (MQ=255)
                                            gCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCGGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaa                                                        <  2:2699125/100‑1 (MQ=255)
                                             cGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaaa                                                       <  2:1557557/100‑1 (MQ=255)
                                                 cAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATc                                                   >  1:565007/1‑100 (MQ=255)
                                                 cAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATc                                                   <  1:1907124/99‑1 (MQ=255)
                                                  aGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAATACGTAAAAAAAAAAAAAAAACGCGTTTTGATCATCACCAAAAATCCa                                                 <  1:44910/100‑1 (MQ=255)
                                                  aGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa                                                 <  2:48037/100‑1 (MQ=255)
                                                  aGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa                                                 <  1:2184783/100‑1 (MQ=255)
                                                    gtgtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCata                                               <  2:2094080/100‑1 (MQ=255)
                                                     tgtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCata                                               <  2:1434902/100‑1 (MQ=255)
                                                     tgtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataa                                              >  2:1425938/1‑100 (MQ=255)
                                                      gtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat                                             >  1:1196894/1‑100 (MQ=255)
                                                      gtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGGTTTGATCATCACCAAAAATCCataat                                             >  1:1049297/1‑100 (MQ=255)
                                                       ttGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat                                             <  1:149227/100‑1 (MQ=255)
                                                       ttGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataata                                            >  2:1961325/1‑100 (MQ=255)
                                                        tGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCAtaataa                                           >  2:626020/1‑100 (MQ=255)
                                                         ggCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCAtaataa                                           >  1:849572/1‑100 (MQ=255)
                                                          gCTTTTGCCGGTAGCGCCAGCGCCACGGCAACGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaat                                         <  1:2903874/100‑1 (MQ=255)
                                                           cTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata                                        <  1:900064/100‑1 (MQ=255)
                                                           cTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata                                        <  2:1147463/100‑1 (MQ=255)
                                                           cTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata                                        >  2:1865714/1‑100 (MQ=255)
                                                           cTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata                                        >  1:2512435/1‑100 (MQ=255)
                                                           cTTTTGCCAGTACGGCCAGTCCCACTGCATCGGCCAACGTCAAAAACGTTAGCCAGCGAGAAAGACTCGTTTTGATCATCACCAAAAATCCATaataaa                                          <  1:780092/99‑1 (MQ=255)
                                                            ttttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCAAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaata                                        <  1:2439043/99‑1 (MQ=255)
                                                              ttGCCGGCAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAAtata                                      >  2:1777556/1‑100 (MQ=255)
                                                               tGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTGAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCATAAATCCATAAGAAATATAAg                                    <  1:3084021/100‑1 (MQ=255)
                                                               tGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg                                    <  2:1858260/100‑1 (MQ=255)
                                                               tGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg                                    >  1:2694716/1‑100 (MQ=255)
                                                                gCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAACTATAAg                                    <  1:51940/100‑1 (MQ=255)
                                                                    gTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAt                               <  1:1619135/100‑1 (MQ=255)
                                                                      agcgccagcgccACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg                             <  2:3118765/100‑1 (MQ=255)
                                                                      agcgccagcgccACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg                             >  1:1564221/1‑100 (MQ=255)
                                                                       gcgccagcgccaCGGCAGCGGCGAACGTAAAAAACGTTAGCCAGGGAGAAAAACGCGTTTGGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg                             <  2:2964072/100‑1 (MQ=255)
                                                                       gcgccagcgccaCGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg                             <  1:1234400/100‑1 (MQ=255)
                                                                          ccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCAAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaat                         <  1:2044910/100‑1 (MQ=255)
                                                                            agcgccaCGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaata                        >  2:1829106/1‑100 (MQ=255)
                                                                              cgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                     <  2:616328/100‑1 (MQ=255)
                                                                              cgccaCGGCAGCGGCGAACGTAAAAACGTTAGCAAGCGATAATAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                     <  1:2377140/100‑1 (MQ=255)
                                                                               gccaCGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                     <  2:1380705/100‑1 (MQ=255)
                                                                               gccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa                    <  2:1279906/100‑1 (MQ=255)
                                                                                ccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATAACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAg                   <  1:690064/100‑1 (MQ=255)
                                                                                ccaCGGAAGCGGCGAACGTCAAAACGTTACACAGCCAGAAAAAAGCGCTATGATCATCACCAAAAATCCATAATAAATATAAGGTCATTGTAATAAAc                     <  1:152836/98‑1 (MQ=255)
                                                                                   cGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATa                >  2:2751430/1‑100 (MQ=255)
                                                                                       aGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGATATTGTAATAAACAGATAAGc             >  1:1876720/1‑100 (MQ=255)
                                                                                       aGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGcc            <  2:1275765/100‑1 (MQ=255)
                                                                                        gcggcgAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGcc            <  1:382525/100‑1 (MQ=255)
                                                                                        gcggcgAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGcc            <  2:822224/100‑1 (MQ=255)
                                                                                         cggcgAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGAAAAGcc            >  1:2861903/1‑99 (MQ=255)
                                                                                          ggcgAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTg          >  2:420241/1‑100 (MQ=255)
                                                                                           gcgAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTgga        <  2:2743155/100‑3 (MQ=255)
                                                                                           gcgAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGaa        <  1:2445151/100‑1 (MQ=255)
                                                                                           gcgAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGaa        <  2:722692/100‑1 (MQ=255)
                                                                                            cgAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGaa        >  1:882478/1‑100 (MQ=255)
                                                                                             gAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTAATTGTAATAAACAGATAAGCCTGAAtt      >  2:3059238/1‑100 (MQ=255)
                                                                                              aaCGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAtt      <  1:292475/100‑1 (MQ=255)
                                                                                              aaCGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTa     <  2:1034336/100‑1 (MQ=255)
                                                                                                 gTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTatg   <  1:1563086/100‑1 (MQ=255)
                                                                                                  tAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTatgg  >  2:2831009/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
ATGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGG  >  NC_000913/1170234‑1170431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: