Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,362 Δ1 bp 16.0% intergenic (+156/+16) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3620A.16.0% 284.9 / 52.6 100intergenic (+156/+16)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base A (41/43);  new base . (11/5);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 105 total aligned reads displayed.

CTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAA  >  NC_000913/1446263‑1446459
                                                                                                    |                                                                                                 
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                   >  1:102878/1‑100 (MQ=255)
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                   >  2:1829983/1‑100 (MQ=255)
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                   >  2:306840/1‑100 (MQ=255)
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                   >  1:133231/1‑100 (MQ=255)
 tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                                  <  2:3313084/100‑1 (MQ=255)
  gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                 <  1:3350022/100‑1 (MQ=255)
  gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                 >  1:1287957/1‑100 (MQ=255)
  gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGAGTTGCAGTACAt                                                                                                  >  1:2329889/1‑99 (MQ=255)
  gAAACAAAGTGAATATTCAAAAACGCCTGTTTAATTTCCAAATGACAAAAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                 <  1:815560/100‑1 (MQ=255)
   aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                                <  1:2811767/100‑1 (MQ=255)
    aaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCa                                                                                               <  1:1711213/100‑1 (MQ=255)
      cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                             <  2:475164/100‑1 (MQ=255)
      cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                             >  2:2049225/1‑100 (MQ=255)
      cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                             <  1:1793026/100‑1 (MQ=255)
      cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                             <  1:2023874/100‑1 (MQ=255)
        aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAACTATCTGCTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                             <  1:2023840/98‑1 (MQ=255)
        aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGc                                                                                           <  1:883745/100‑1 (MQ=255)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                          <  2:44181/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                          <  2:2909581/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                          <  2:3156099/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                          >  2:1504505/1‑100 (MQ=255)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                          >  1:122065/1‑100 (MQ=255)
           tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCaa                                                                                        <  1:2006913/100‑1 (MQ=38)
           tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCaa                                                                                        >  2:1384607/1‑100 (MQ=255)
            gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAt                                                                                       >  1:2349861/1‑100 (MQ=255)
             aaTATGAAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATATTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                      <  1:775854/100‑1 (MQ=255)
              atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                     <  2:2498116/100‑1 (MQ=255)
               tatTCAAAAACTCCTGTCAAATTGCCTTTTGCTCTGAAAAATGCATAGTCAAATATCTGTTTTACCTAATTGGCGTTGCAGTACATGCAACGCCAAttag                                                                                    >  1:2405353/1‑100 (MQ=255)
               tatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAttag                                                                                    <  1:1557171/100‑1 (MQ=37)
               tatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                     <  2:1185913/99‑1 (MQ=255)
                  tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtta                                                                                 >  2:2104735/1‑100 (MQ=255)
                  tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                                  <  1:930028/99‑1 (MQ=255)
                     aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                              <  2:1507033/100‑1 (MQ=255)
                           ccTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAttt                                                                        >  1:249859/1‑100 (MQ=255)
                            cTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTa                                                                       >  1:2809698/1‑100 (MQ=255)
                             tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTAc                                                                      >  2:1423346/1‑100 (MQ=255)
                             tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATGGGCGTTGCAGTACATACAACGCCAATTAGTTAGCGGAATTTa                                                                       >  1:1124319/1‑99 (MQ=255)
                                cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                   <  2:3081804/100‑1 (MQ=255)
                                    ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa                                                               <  2:1585041/100‑1 (MQ=255)
                                        cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                           <  2:2214669/100‑1 (MQ=255)
                                        cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                           >  2:1975943/1‑100 (MQ=255)
                                             gCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCt                                                        >  1:2800587/1‑98 (MQ=255)
                                              cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                     <  2:1065282/100‑1 (MQ=255)
                                              cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                     >  2:693447/1‑100 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                    <  2:207774/100‑1 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                    <  2:2758061/100‑1 (MQ=255)
                                                 tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTc                                                  <  1:112089/100‑1 (MQ=255)
                                                   aaaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCAt                                                >  2:1176858/1‑100 (MQ=255)
                                                     aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATcc                                              <  2:946823/100‑1 (MQ=255)
                                                     aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATcc                                              <  1:3111010/100‑1 (MQ=255)
                                                      aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGACGTCATccc                                             >  2:3383351/1‑100 (MQ=255)
                                                      aaTGCATAGTCAAATATCTGTTTTAACTAAGTGGCGTTGCAGTACATGCAACGCCAAGTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATccc                                             <  2:1433410/100‑1 (MQ=255)
                                                          cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                         <  2:2770975/100‑1 (MQ=255)
                                                          cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                         <  2:249860/100‑1 (MQ=255)
                                                           aTAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                        <  1:2593970/100‑1 (MQ=255)
                                                           aTAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                        <  1:2575412/100‑1 (MQ=255)
                                                            tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                       >  2:38095/1‑100 (MQ=255)
                                                            tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                       >  2:3308597/1‑100 (MQ=255)
                                                               tCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTg                                    <  1:873748/100‑1 (MQ=255)
                                                                 aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCTAATTAGTTAGCGGAATTTACGTCGACACTCGCCTGGCGTCATCCCAAAGCGTTGc                                   >  1:1369761/1‑99 (MQ=255)
                                                                 aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                  <  2:542856/100‑1 (MQ=255)
                                                                 aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                  <  1:3161147/100‑1 (MQ=255)
                                                                     atCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                              <  2:2232354/100‑1 (MQ=255)
                                                                     atCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                              >  1:1637354/1‑100 (MQ=255)
                                                                      tCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAAt                             <  2:1124322/100‑1 (MQ=255)
                                                                       cTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATa                            <  1:2452556/100‑1 (MQ=255)
                                                                        tGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                           >  2:2892153/1‑100 (MQ=255)
                                                                        tGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTAGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                           >  1:2697591/1‑100 (MQ=255)
                                                                         gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTGCGTCGATACGCGCCTGGAGTCATCCCAAAGCTGTACTTAAATAcc                          >  1:641682/1‑100 (MQ=38)
                                                                         gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                          >  1:1006338/1‑100 (MQ=255)
                                                                         gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                          >  2:1296187/1‑100 (MQ=255)
                                                                         gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTAATCCCAAAGCGTTGCTTAAATAcc                          >  1:2885213/1‑100 (MQ=255)
                                                                          ttttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAACTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg                         >  2:2178475/1‑100 (MQ=255)
                                                                          cgctAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg                         >  2:1561109/4‑100 (MQ=25)
                                                                           gctAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTAAGTAGGTAGCGCCCAGGGGTCTGCCCAAAGCGTTGCTTGAATACCGt                        >  1:1940196/3‑100 (MQ=9)
                                                                            ttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGtt                       >  2:1471294/1‑100 (MQ=255)
                                                                             tAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                      <  1:3005980/100‑1 (MQ=255)
                                                                                 tAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaa                  <  2:2020163/100‑1 (MQ=255)
                                                                                  aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa                 >  2:2257339/1‑100 (MQ=255)
                                                                                   aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                >  2:880485/1‑100 (MQ=38)
                                                                                   aTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaa                  >  2:2983001/1‑98 (MQ=255)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa              >  1:3195099/1‑100 (MQ=38)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa              >  2:265737/1‑100 (MQ=38)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa              >  2:928287/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTAGGTCGATACTGGCCTGGCGTTATCCGAAAGCATGGCGTAAATAGCGTTGAAAAATGAc             >  1:831876/1‑100 (MQ=21)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc             >  2:1100853/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc             >  1:1467322/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc             >  1:3315487/1‑100 (MQ=38)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc             <  2:3074783/100‑1 (MQ=255)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc             >  2:547864/1‑100 (MQ=255)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc             >  2:1880680/1‑100 (MQ=255)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc             >  2:2011679/1‑100 (MQ=255)
                                                                                           tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg        <  1:1874067/100‑1 (MQ=255)
                                                                                           tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg        <  1:2248071/100‑1 (MQ=255)
                                                                                            gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt       <  2:360896/100‑1 (MQ=255)
                                                                                             cAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCACCTGGCGTCATCCCAAAGCGTTGCTTAAATACCCTTGAAAAATGACTCTGGTc      >  1:168005/1‑100 (MQ=255)
                                                                                              aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa     >  1:3273894/1‑100 (MQ=255)
                                                                                              aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa     >  2:2049546/1‑100 (MQ=255)
                                                                                              aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCGGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa     >  1:999525/1‑100 (MQ=255)
                                                                                                  aCATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCTCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaa  <  1:1167561/100‑1 (MQ=255)
                                                                                                    |                                                                                                 
CTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAA  >  NC_000913/1446263‑1446459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: