Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,469,656:1 (T)7→8 100% coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,6491.T94.9% 511.3 / 19.1 157coding (519/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (3/5);  new base T (71/78);  total (74/83)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 175 total aligned reads displayed.

AATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469550‑2469743
                                                                                                    |                                                                                                
aaTGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTa                                                                                                   >  2:1868350/1‑100 (MQ=255)
 aTGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAt                                                                                                 >  2:551298/1‑100 (MQ=255)
       gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         <  2:869120/100‑1 (MQ=255)
         atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttc                                                                                       <  2:2559328/100‑3 (MQ=255)
         atcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttc                                                                                       <  2:2823042/100‑3 (MQ=255)
          tcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcg                                                                                      <  2:1513377/100‑4 (MQ=255)
           catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgt                                                                                     <  2:567989/100‑5 (MQ=255)
           catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgt                                                                                     <  1:2026535/100‑5 (MQ=255)
           catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgt                                                                                     <  2:2664679/100‑5 (MQ=255)
           catTCTATCAAGAGCAAATAGTTATAATTTTCCTTCAATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgt                                                                                     >  1:2485855/1‑96 (MQ=255)
            atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgtt                                                                                    >  2:1207985/1‑95 (MQ=255)
            atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgtt                                                                                    <  2:774056/100‑6 (MQ=255)
              tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTTGTGTATTAAAAAAAATAAAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   <  1:1020407/100‑1 (MQ=255)
              tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   <  2:394851/100‑1 (MQ=255)
               cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  >  2:2927501/1‑100 (MQ=255)
               cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  <  1:2385821/100‑1 (MQ=255)
                tATCAAGGGCAAAGATTTATAATGTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCta                                                                                 <  1:3355462/100‑1 (MQ=255)
                tATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCta                                                                                 >  2:1827805/1‑100 (MQ=255)
                    aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                             >  1:1760359/1‑100 (MQ=255)
                    aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTCTTCGTTGCtaataa                                                                             >  1:2238033/1‑100 (MQ=255)
                     agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTactaat                                                                            >  1:919051/1‑100 (MQ=255)
                     agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            >  2:525543/1‑100 (MQ=255)
                     agagCAAATAGTTACAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            <  1:961243/100‑1 (MQ=255)
                     aaaacAAATAGTTATAATTTTCCTTCCAATTTTTGTGTATTCATAAAAAAACAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                             <  1:492593/95‑1 (MQ=255)
                      gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                           <  2:3308381/100‑1 (MQ=255)
                       agCAAATAGTTATAATTTTCCTTCCATTTTTTTTTTTTTTTTTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTTCTAATTatat                                                                          >  1:3222676/1‑100 (MQ=255)
                        gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATc                                                                         <  1:3120793/100‑1 (MQ=255)
                        gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATc                                                                         >  1:1355795/1‑100 (MQ=255)
                        gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCt                                                                        <  2:1267523/100‑1 (MQ=255)
                         naaaTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTCTAATATTATCt                                                                        >  1:371737/2‑100 (MQ=255)
                         cAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCt                                                                        >  1:1288605/1‑100 (MQ=255)
                             tAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTg                                                                    <  2:2658001/100‑1 (MQ=255)
                              aGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGc                                                                   >  2:2216480/1‑100 (MQ=255)
                                ttATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTa                                                                 <  1:3019608/100‑1 (MQ=255)
                                 tataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaa                                                                >  2:3206160/1‑100 (MQ=255)
                                  ataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaa                                                               <  1:2706284/100‑1 (MQ=255)
                                   taATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaa                                                                >  1:3191669/1‑98 (MQ=255)
                                     aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  2:885877/100‑1 (MQ=255)
                                     aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:1940252/100‑1 (MQ=255)
                                     aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTAGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:1855265/100‑1 (MQ=255)
                                     aTTTTACTCCCATTTTTTGTTTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:1555273/100‑1 (MQ=255)
                                      ttttCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATc                                                           <  1:702464/100‑1 (MQ=255)
                                       tttCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCa                                                          >  2:3138095/1‑100 (MQ=255)
                                       cttccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTGGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCa                                                          >  1:1482445/2‑100 (MQ=255)
                                         tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAt                                                        <  2:3108658/100‑1 (MQ=255)
                                         tccttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTACTAATAATATCTACTGCTAAAAATCatt                                                        >  1:3128278/1‑98 (MQ=255)
                                             tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtaa                                                    >  2:2724454/1‑99 (MQ=255)
                                              ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  1:2053297/100‑1 (MQ=255)
                                                aTTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATAtt                                                 >  2:23285/1‑100 (MQ=255)
                                                     tGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAccc                                            >  2:1783522/1‑100 (MQ=255)
                                                       gtgtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCa                                          <  1:2824083/100‑1 (MQ=255)
                                                         gtATTCATTAACATGCAAAAACAATAAATCTATTCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        <  1:1131491/100‑1 (MQ=255)
                                                         gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTATTAATATCTACTGCTAAAAATCAATTTATATTAACTCCACt                                        >  1:2903261/1‑100 (MQ=255)
                                                         gtATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACt                                        <  2:756620/100‑1 (MQ=255)
                                                          tATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTTCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCTCACTa                                       >  1:2568825/1‑100 (MQ=255)
                                                             tCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    <  1:166621/100‑1 (MQ=255)
                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATa                                    >  2:1036260/1‑99 (MQ=255)
                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  1:3291775/1‑100 (MQ=255)
                                                               aTTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   <  2:396978/99‑1 (MQ=255)
                                                               aTTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   >  2:2418505/1‑99 (MQ=255)
                                                                ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtg                                 <  2:2768873/100‑1 (MQ=255)
                                                                ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtg                                 <  2:346673/100‑1 (MQ=255)
                                                                   ataTGCAAAATTAATTAATCTATTCTATTGCTATTTTTTTTCGTTGCTAATAATATCTAACACTAAAAATCAATTTATATTAACCCCACTAATAGTGTa                               <  1:2320728/97‑1 (MQ=255)
                                                                   aCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                              >  2:146801/1‑100 (MQ=255)
                                                                    cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTTTTAATATCTAATGCTAAAAATCAATTTTTATTAACCCCACTAATAGTGTAtt                             >  1:210747/1‑100 (MQ=255)
                                                                    cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             >  2:16433/1‑100 (MQ=255)
                                                                    cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             <  2:14186/100‑1 (MQ=255)
                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             >  1:54683/1‑98 (MQ=255)
                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           >  2:2995570/1‑100 (MQ=255)
                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           <  1:2985809/100‑1 (MQ=255)
                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           <  1:1317174/100‑1 (MQ=255)
                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           <  2:789415/100‑1 (MQ=255)
                                                                      tGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           >  2:1706789/1‑100 (MQ=255)
                                                                       gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCat                          >  2:1943848/1‑100 (MQ=255)
                                                                       gCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCa                           <  2:2840793/99‑1 (MQ=255)
                                                                        cAAAAACAATAAGTCTATGCTTTTGCTATATGTTTTCGTTGCTAATAATATCTAATGCTGAAAATCAATTTATAGGAACCCAACAATGAGTGTGTTCa                           <  1:2092216/98‑1 (MQ=255)
                                                                        cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  2:1576905/1‑100 (MQ=255)
                                                                        cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         <  2:665664/100‑1 (MQ=255)
                                                                        cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         >  1:2182992/1‑100 (MQ=255)
                                                                        cAAAAACAATAAATCTATGCTATTGATATTTTTTTTAGTTTCTAATGATATTTAATGCTACAAATCATTTTATATTAAAACCACTAATACGGTATTCata                         >  1:673006/1‑100 (MQ=255)
                                                                         aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        >  1:1051997/1‑100 (MQ=255)
                                                                         aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        >  1:2433346/1‑100 (MQ=255)
                                                                         aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCata                         <  1:1028012/99‑1 (MQ=255)
                                                                         aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatt                       >  1:2434417/1‑100 (MQ=255)
                                                                          aaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatt                       <  1:3048732/100‑1 (MQ=255)
                                                                          aaaaCAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatatta                      <  1:1607748/100‑1 (MQ=255)
                                                                                 tAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTCTTCATATTATAtttt                >  2:3343411/1‑100 (MQ=255)
                                                                                 tAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttt                >  1:150080/1‑100 (MQ=255)
                                                                                   aaTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt              >  1:1678160/1‑100 (MQ=255)
                                                                                   aaTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt              <  1:2216465/100‑1 (MQ=255)
                                                                                     tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  2:3014144/100‑1 (MQ=255)
                                                                                     tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  2:1887348/100‑1 (MQ=255)
                                                                                     tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  2:1094145/100‑1 (MQ=255)
                                                                                      cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  2:2813686/100‑1 (MQ=255)
                                                                                         tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  1:2821805/100‑1 (MQ=255)
                                                                                          gCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg       >  2:472484/1‑100 (MQ=255)
                                                                                           cTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa      <  1:385351/100‑1 (MQ=255)
                                                                                            ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGa      <  1:1416857/99‑1 (MQ=255)
                                                                                               tGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCGTATTATATTTTTTTTGATAGAcaca  <  1:1946772/100‑1 (MQ=255)
                                                                                               tGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGAcaca  <  2:1760370/100‑1 (MQ=255)
                                                                                                    |                                                                                                
AATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469550‑2469743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: