BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,563,518:1	(A)_5→6	100%	intergenic (+401/‑74)	yffP → / → yffQ	CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,563,513	1	.	A	96.1%	429.4 / 9.0	127	intergenic (+396/‑79)	yffP/yffQ	CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand): ref base . (4/1); new base A (65/57); total (69/58)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑A‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAA  >  NC_000913/2563415‑2563604

ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a                                                                                               <  2:2778780/100‑1 (MQ=255)

ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a                                                                                               >  1:1441854/1‑100 (MQ=255)

ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a                                                                                               >  1:1820608/1‑100 (MQ=255)

  tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a‑a‑a                                                                                           >  1:117276/1‑100 (MQ=255)

  tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAAAAAAATAAAGATGGGAGGT‑a‑a‑a                                                                                           >  1:544311/1‑100 (MQ=255)

       ttttTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGAAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a‑a‑a‑aaatg                                                                                     <  1:2312431/100‑4 (MQ=255)

       tttGTGATGCAGATCGCTTTTTCCCTGCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a‑a‑a‑aaatg                                                                                     <  1:1033103/100‑4 (MQ=255)

        ttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a‑a‑a‑aaatga                                                                                    <  1:507620/100‑5 (MQ=255)

        ttGTGATGCAGATCGCTTTTTCACTTCAGGCATTTTTTTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑AGA‑A‑AATGa                                                                                     >  1:1815249/1‑100 (MQ=255)

         tgtgATGCAGGTCGCTTTTTCCCTTCAGGCATTTTAGTTTAATGCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a‑a‑a‑aaatgaa                                                                                   <  1:298247/100‑6 (MQ=255)

         tgtgATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTACCCAAAAACAAAATAAAGATGGGAGGT‑a‑a‑a‑aaatgan                                                                                   <  1:1815504/100‑6 (MQ=255)

         tgtgATGCAGATCGCTTTTTCCCTTCAGGCATCTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑a‑a‑a‑aaatgaa                                                                                   <  1:609773/100‑6 (MQ=255)

          gtgATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGaaa                                                                                   >  2:1376946/1‑100 (MQ=255)

             aTGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACgg                                                                                <  2:1441860/100‑1 (MQ=255)

              tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGt                                                                               >  1:2709580/1‑100 (MQ=255)

              tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGt                                                                               >  2:2026498/1‑100 (MQ=255)

              tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGt                                                                               >  2:2566766/1‑100 (MQ=255)

               gCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTg                                                                              <  1:1229254/100‑1 (MQ=255)

                cAGATCGTTTTTTTCCTTAAGGCATTTTTGTTTAATTCAATCAATGAAAGGCAGTTCTCAATATCAAAATAAAGATGGGAGGT‑A‑ATA‑AATGAAACGGTg                                                                              >  1:2394403/1‑99 (MQ=255)

                cAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTgg                                                                             <  1:791900/100‑1 (MQ=255)

                 aGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCAAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACg                                                                                 >  1:1276865/1‑95 (MQ=255)

                  gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGa                                                                           >  2:1182261/1‑100 (MQ=255)

                  gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGa                                                                           >  2:1609507/1‑100 (MQ=255)

                   aTCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGaa                                                                          <  2:451697/100‑1 (MQ=255)

                      gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTg                                                                       >  2:1711539/1‑100 (MQ=255)

                      gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTg                                                                       <  1:2508874/100‑1 (MQ=255)

                      gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTg                                                                       >  2:1711553/1‑100 (MQ=255)

                       cTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTgg                                                                      >  2:701358/1‑100 (MQ=255)

                             cccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTAc                                                                >  1:804771/1‑100 (MQ=255)

                             cccTTCAGGCATTTTTGTTTAATTCAAGCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTAc                                                                >  1:805016/1‑100 (MQ=255)

                              ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACt                                                               >  2:2037062/1‑100 (MQ=255)

                               cTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTg                                                              >  2:2119190/1‑100 (MQ=255)

                                 tCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTAc                                                                <  1:2856052/96‑1 (MQ=255)

                                 tCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGt                                                            <  1:930114/100‑1 (MQ=255)

                                  cAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                           <  1:3262002/100‑1 (MQ=255)

                                   aGGCATTTTTGTTTAAATCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTg                                                           <  2:3034693/99‑1 (MQ=255)

                                    ggCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGAt                                                         <  2:39066/100‑1 (MQ=255)

                                    ggCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGAt                                                         >  2:2551698/1‑100 (MQ=255)

                                     gCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATg                                                        <  2:1606464/100‑1 (MQ=255)

                                      cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGAGAAGTGGCTTTACTGGTGATgg                                                       >  1:2872206/1‑100 (MQ=255)

                                       aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCTACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGt                                                      <  2:3226644/100‑1 (MQ=255)

                                       aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATg                                                        <  1:1771672/98‑1 (MQ=255)

                                       aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGt                                                      <  2:635418/100‑1 (MQ=255)

                                          tttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAAt                                                   >  2:343650/1‑100 (MQ=255)

                                              tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg                                               >  2:139875/1‑100 (MQ=255)

                                               ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                              <  1:953290/100‑1 (MQ=255)

                                               ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                              >  1:310124/1‑100 (MQ=255)

                                               ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                              >  2:446157/1‑100 (MQ=255)

                                               ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                              <  2:531638/100‑1 (MQ=255)

                                                 aaTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGtt                                            >  2:569037/1‑100 (MQ=255)

                                                  aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt                                           <  2:2849188/100‑1 (MQ=255)

                                                  aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGAAGGTAATGTTGGttt                                           >  1:1940735/1‑100 (MQ=255)

                                                  aTTCAAGCAACGAAAGGCAGTTCCCAAGAACAAAAAAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt                                           <  1:2146689/100‑1 (MQ=255)

                                                   ttCCATCAACGAAAGGCATTTCCCAACAACAAAAGAAAGATGGGAGGG‑A‑A‑ACAATGAAACGGTGGGAAGTGGCTTTACTGGTGACGGTAATGTGGGttt                                           >  1:159122/1‑99 (MQ=255)

                                                   ttCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTg                                          <  2:787661/100‑1 (MQ=255)

                                                   ttCAAGCAAAGAAAGGCAGTGCCCAACAACAAAAGAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgggt                                            <  1:2623950/98‑3 (MQ=255)

                                                    tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc                                         <  1:2522066/100‑1 (MQ=255)

                                                     caatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCt                                        >  2:1545244/1‑100 (MQ=255)

                                                     caatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGAGGGGAGGTAA‑A‑A‑AATGAAACGGGGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCt                                        >  2:154934/1‑100 (MQ=255)

                                                      aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                       <  2:1397421/100‑1 (MQ=255)

                                                      aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                       <  2:2050153/100‑1 (MQ=255)

                                                      aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                       >  1:1688150/1‑100 (MQ=255)

                                                      aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                       >  2:672850/1‑100 (MQ=255)

                                                       atcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGt                                      >  1:1072085/1‑100 (MQ=255)

                                                       atcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGt                                      <  2:2447959/100‑1 (MQ=255)

                                                        tcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTa                                     <  2:52117/100‑1 (MQ=255)

                                                         caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                    >  1:3374453/1‑100 (MQ=255)

                                                          aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                   <  2:862843/100‑1 (MQ=255)

                                                          aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                   >  2:3305745/1‑100 (MQ=255)

                                                          aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                   >  1:2083166/1‑100 (MQ=255)

                                                          aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                   >  1:3046778/1‑100 (MQ=255)

                                                           aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGGGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                   <  1:2117331/99‑1 (MQ=255)

                                                           aCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑A‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGa                                 <  2:3013735/100‑1 (MQ=255)

                                                              aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc                               <  2:1805946/100‑1 (MQ=255)

                                                                aGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtg                             <  2:1718897/100‑1 (MQ=255)

                                                                 ggCAGTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgt                            >  2:1377707/1‑100 (MQ=255)

                                                                     gTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAag                        >  2:1820376/1‑100 (MQ=255)

                                                                     gTTCCCAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAag                        <  1:701355/100‑1 (MQ=255)

                                                                        cccAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                     >  2:263841/1‑100 (MQ=255)

                                                                        cccAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                     <  2:102015/100‑1 (MQ=255)

                                                                        cccAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                     >  1:2442781/1‑100 (MQ=255)

                                                                        cccAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                     <  2:303114/100‑1 (MQ=255)

                                                                         ccAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg                    >  2:1092397/1‑100 (MQ=255)

                                                                         ccAACAACAAAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg                    >  1:57365/1‑100 (MQ=255)

                                                                          caacaacaaAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAggagga                   >  1:1092286/1‑100 (MQ=255)

                                                                             caacaaAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc                >  1:276727/1‑100 (MQ=255)

                                                                             caacaaAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc                >  1:2646938/1‑100 (MQ=255)

                                                                               acaaAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa              <  1:407205/100‑1 (MQ=255)

                                                                               acaaAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa              <  1:154934/100‑1 (MQ=255)

                                                                               acaaAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACGGTGAAGGAGGACGCCa              >  1:1980270/1‑100 (MQ=255)

                                                                                caaAATAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt             <  2:2865464/100‑1 (MQ=255)

                                                                                 aaaaTAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATg            <  2:2709602/100‑1 (MQ=255)

                                                                                  aaaTAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGa           >  2:2187419/1‑100 (MQ=255)

                                                                                   aaTAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGaa          >  2:1618166/1‑100 (MQ=255)

                                                                                     tAAAGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc        <  2:3256525/100‑1 (MQ=255)

                                                                                       aaGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtt      >  1:16182/1‑100 (MQ=255)

                                                                                       aaGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtt      >  2:687468/1‑100 (MQ=255)

                                                                                        aGATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACttt     <  2:478443/100‑1 (MQ=255)

                                                                                         gATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTTAACTGTGAAGGAGGACGCCATGAAACtttt    >  1:395268/1‑100 (MQ=255)

                                                                                         gATGGGAGGTAA‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt    <  1:1376940/100‑1 (MQ=255)

GGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGT‑A‑A‑A‑AATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAA  >  NC_000913/2563415‑2563604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`