Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 497,139:1 (T)5→6 100% intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913497,1341.T93.0% 367.8 / 20.2 115intergenic (+140/‑41)htpG/adkprotein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
Reads supporting (aligned to +/- strand):  ref base . (2/6);  new base T (41/66);  total (43/72)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497035‑497228
                                                                                                     |                                                                                               
tttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGc                                                                                                  <  1:3353929/100‑1 (MQ=255)
 ttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCt                                                                                                <  2:3117565/100‑1 (MQ=255)
  tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTCTCGCtt                                                                                               >  1:487689/1‑100 (MQ=255)
  tcatcaTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtt                                                                                               <  1:1518861/100‑1 (MQ=255)
   catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttt                                                                                             >  2:110043/1‑100 (MQ=255)
    atcatcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttt                                                                                            >  2:1625375/1‑100 (MQ=255)
     tcatcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttt                                                                                           <  2:1160689/100‑1 (MQ=255)
       atctcgaCTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttttt                                                                                          <  1:3079033/93‑2 (MQ=255)
       atcTGCACTTTCCTCAGATTAATTCGCCATTAACCGTTTCAGCGCCAGGTGCGTTTCTTGAGGCAATCGCCTGTTAGTGGTATCGTTTAGCGCttttttc                                                                                         <  1:2040955/100‑3 (MQ=255)
        tcggCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTTCCTTTCTTGCGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtcttttc                                                                                         >  1:3214522/4‑97 (MQ=255)
         cTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaa                                                                                       >  1:2293900/1‑96 (MQ=255)
          tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                      >  1:102114/1‑95 (MQ=255)
          tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                      <  2:1612438/100‑6 (MQ=255)
          tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                      <  2:1442580/100‑6 (MQ=255)
           gCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCACTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCaaaa                                                                                      >  1:1514266/1‑100 (MQ=255)
           gCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCaaaa                                                                                      <  2:848661/100‑1 (MQ=255)
           gCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTcaaaa                                                                                     <  1:2270674/100‑1 (MQ=255)
              cTTTCCGTAAATTATCTTGTCATTATCCGTTTCAGCCCCTGGTGTCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTcaaaa                                                                                     <  1:2796230/97‑1 (MQ=255)
              cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   <  1:1755745/100‑1 (MQ=255)
              cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   >  1:2682366/1‑100 (MQ=255)
              cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   >  1:2655978/1‑100 (MQ=255)
              cTTTCCGAAAATTATCTCGCCATTAACCGTTTCAGACAAAGGTGCCTTTCTTGAGGCAATCGACTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAAt                                                                                   <  1:2585479/100‑1 (MQ=255)
                ttCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTc                                                                                 <  1:1349871/100‑1 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGTCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  2:1647650/100‑1 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                >  1:1381369/1‑100 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  2:1535573/100‑1 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  2:1632438/100‑1 (MQ=255)
                 tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  1:1398034/100‑1 (MQ=255)
                   ngCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGaca                                                                             >  2:1512467/2‑100 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                >  1:2051283/1‑98 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              <  2:503175/100‑1 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              >  2:2251683/1‑100 (MQ=255)
                   cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              <  1:3031397/100‑1 (MQ=255)
                         ttATCTCGCCATTAACCGTTTCAGCCCCAGGTGCTTTTCTTGAGGTAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAttt                                                                        <  1:244640/100‑1 (MQ=255)
                         ttATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAttt                                                                        <  2:723379/100‑1 (MQ=255)
                         ttATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACAttt                                                                        >  2:1681069/1‑100 (MQ=255)
                           aTCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTa                                                                      <  2:200717/100‑1 (MQ=255)
                              tcGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAgg                                                                   <  1:2004133/100‑1 (MQ=255)
                               cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAggg                                                                  >  1:2548726/1‑100 (MQ=255)
                                gCCATTAACCGGTTCAGCCCCATGTGCCTTTCTTGAGGCAATCGCCTGGTGGTGGGATCGTTTATCGCTTTTTTCAGAAAATTCGACACATTTTAAtggg                                                                 <  1:2892584/100‑1 (MQ=255)
                                     tAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAtttt                                                            >  2:1174209/1‑100 (MQ=255)
                                      aaCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           <  2:429716/100‑1 (MQ=255)
                                      aaCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           <  2:277262/100‑1 (MQ=255)
                                      aaCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           >  2:2524390/1‑100 (MQ=255)
                                       tctgTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCg                                                          <  2:875737/97‑1 (MQ=255)
                                        ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCg                                                          <  2:3377481/99‑1 (MQ=255)
                                        ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  1:2347540/100‑1 (MQ=255)
                                         cGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCa                                                        <  2:1848114/100‑1 (MQ=255)
                                         cGTATCAGCCCCAGGTGCCCTTCTCGAGGCAAAAGCCTGTTGGTGGTATCGTTTATAAAAAAAAAAAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  1:644968/99‑1 (MQ=255)
                                          gTTTCAGCCCTAGGTGCCTTTGTTTAGGTAATCGCCTGTTGGTGGTATCTTTTAGCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCa                                                        <  1:3198150/99‑1 (MQ=255)
                                          gTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCgaga                                                       >  1:875734/1‑97 (MQ=255)
                                          gTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         >  1:963158/1‑98 (MQ=255)
                                           tttCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATg                                                     <  1:1751937/100‑1 (MQ=255)
                                             tCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCTTAAAATTTGACACATTTTAAGGGGATTTTCGCAATGc                                                    >  1:2607077/1‑100 (MQ=255)
                                             tCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGc                                                    >  2:724274/1‑100 (MQ=255)
                                             tCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGc                                                    <  2:1434186/100‑1 (MQ=255)
                                              cagatccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  1:419465/95‑1 (MQ=255)
                                              cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAGAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  1:1183854/100‑1 (MQ=255)
                                              cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  2:1910908/100‑1 (MQ=255)
                                               aGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGt                                                  >  1:2832598/1‑100 (MQ=255)
                                                 ccccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTAt                                                <  2:3037730/100‑1 (MQ=255)
                                                   ccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCa                                              >  1:1652563/1‑100 (MQ=255)
                                                         gCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGc                                        >  2:349390/1‑100 (MQ=255)
                                                           cTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCtt                                      >  2:555532/1‑100 (MQ=255)
                                                           cTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCtt                                      <  2:1731781/100‑1 (MQ=255)
                                                             ttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTgg                                    <  1:3360606/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  2:1733298/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  1:1701538/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  2:2113368/100‑1 (MQ=255)
                                                              tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATCTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  1:154635/100‑1 (MQ=255)
                                                               cTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCTTTATTTTTTACACTTTTTTAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg                                  >  2:482314/1‑100 (MQ=255)
                                                               cTTGAGGCAATCGCCTGTTGCTGGTATCTTGTATCGCTTCTTTCCACAAATTCGACACATTGTAAGCGCATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  1:1376963/99‑1 (MQ=255)
                                                                ttGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcgc                                 >  1:1267682/1‑100 (MQ=255)
                                                                    ggCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCg                             <  1:925690/100‑1 (MQ=255)
                                                                      naaTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCggg                           >  2:690945/2‑100 (MQ=255)
                                                                      cAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCgg                            >  1:1389785/1‑99 (MQ=255)
                                                                        aTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCggg                           >  1:1826498/1‑98 (MQ=255)
                                                                         tCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcgc                        <  2:1699066/100‑1 (MQ=255)
                                                                          cGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGcgcg                       >  2:41851/1‑100 (MQ=255)
                                                                           gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      <  1:227287/100‑1 (MQ=255)
                                                                            ccgatctgtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCggg                     <  2:2793864/93‑1 (MQ=255)
                                                                            ccTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCggg                     <  2:2802283/100‑1 (MQ=255)
                                                                            ccTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCggg                     <  2:3208386/100‑1 (MQ=255)
                                                                             cTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggg                    >  2:972044/1‑100 (MQ=255)
                                                                               gTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGaa                  >  2:2197710/1‑100 (MQ=255)
                                                                                 tggtggTATCGTTTATCGCTTTTTTCAAAAAATTCGGCCCATTTTAAGGGGATTTGCGCAAGGCGTAGCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAg                <  1:546148/100‑1 (MQ=255)
                                                                                  ggtggtATCGTTTATCGCTTTTTTCCAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               <  2:410529/100‑1 (MQ=255)
                                                                                  ggtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               <  2:2746446/100‑1 (MQ=255)
                                                                                  ggtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAgg               <  2:924108/100‑1 (MQ=255)
                                                                                   gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggagatcgg              >  1:2793841/1‑93 (MQ=255)
                                                                                      gtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACt           <  1:2673335/100‑1 (MQ=255)
                                                                                      gtATCGTTTATCGCTTTTTTCAAAAAATTCGACACAGTTTAAGGCGATTGTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACt           <  1:691483/100‑1 (MQ=255)
                                                                                         tCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAg        <  1:110043/100‑1 (MQ=255)
                                                                                          cGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAgg       <  2:253660/100‑1 (MQ=255)
                                                                                           gTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGc      <  1:1988920/100‑1 (MQ=255)
                                                                                           gTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGACTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGCAAGGGACTCAg        >  1:2944121/1‑98 (MQ=255)
                                                                                             ttATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTc    <  2:833787/100‑1 (MQ=255)
                                                                                              tATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCa   >  1:1722132/1‑100 (MQ=255)
                                                                                               aTCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAg  >  2:3332594/1‑100 (MQ=255)
                                                                                               aTCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAg  <  1:507544/100‑1 (MQ=255)
                                                                                                     |                                                                                               
TTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497035‑497228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: