Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 922,316:1 +C 16.2% intergenic (+23/+50) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913922,3141.C16.2% 367.0 / 78.0 111intergenic (+21/+52)macB/cspDmacrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base . (39/54);  new base C (9/9);  total (48/63)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-02

Only 100 of 113 total aligned reads displayed.

CCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTG  >  NC_000913/922217‑922413
                                                                                                  |                                                                                                   
ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGcc                                                                                                   <  2:627871/100‑1 (MQ=255)
      ttttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCgg                                                                                             >  2:1818854/1‑100 (MQ=255)
      ttttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCgg                                                                                             >  2:376828/1‑100 (MQ=255)
         tATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCt                                                                                          <  1:1092095/100‑1 (MQ=255)
           tttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTgg                                                                                        >  1:3054769/1‑100 (MQ=255)
           tttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTgg                                                                                        >  2:3145376/1‑100 (MQ=255)
            ttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGc                                                                                       <  2:9587/100‑1 (MQ=255)
             tGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCa                                                                                      >  1:1728709/1‑100 (MQ=255)
             tGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCa                                                                                      <  1:2316896/100‑1 (MQ=255)
             tGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCa                                                                                      >  2:2478093/1‑100 (MQ=255)
              ggCTGGTTACCCGCACGAAATGCGGCACGACTGGATGCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAt                                                                                     <  1:1493646/100‑1 (MQ=255)
               gCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtt                                                                                    <  2:805464/100‑1 (MQ=39)
               gCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAtt                                                                                    >  2:455287/1‑100 (MQ=255)
                ctagTTACCCGCACGAAATGCGGCACTACTTGATCCAGTAGATGCTCTCGCACGAGAGTAATTTTTGAAATAAAAATGCCAGCCGATCGCGCTGGCAt                                                                                     <  1:2821066/95‑1 (MQ=255)
                cTGGTTACCCGCACGAAGTGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAttt                                                                                   <  1:869123/100‑1 (MQ=38)
                cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAttt                                                                                   <  2:1759106/100‑1 (MQ=39)
                 tGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttt                                                                                  <  2:1384123/100‑1 (MQ=39)
                  ggTTACCCGCACGAAATGCGGCACGACTGGGTCCAGTAGATGCTCGGGCACGAGAGTAGTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                 <  1:1283884/100‑1 (MQ=255)
                  ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                 <  2:2098324/100‑1 (MQ=255)
                  ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                 <  2:575304/100‑1 (MQ=255)
                  ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                 <  2:1564798/100‑1 (MQ=255)
                  ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                 <  2:3311775/100‑1 (MQ=255)
                   gTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAttttta                                                                                <  2:3270562/100‑3 (MQ=38)
                    ttACCCGCACGAAATGCGGCACGACTGGATCCAGTATATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGc                                                                                <  1:1659427/99‑1 (MQ=255)
                    ttACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGcc                                                                               <  1:3350508/100‑1 (MQ=255)
                     tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatc                                                                              <  1:2814471/100‑5 (MQ=38)
                     tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGcc                                                                               <  2:3006748/99‑1 (MQ=255)
                       cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatctc                                                                            <  2:177543/100‑7 (MQ=37)
                       cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCttt                                                                            >  2:603779/1‑100 (MQ=255)
                        ccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTa                                                                           <  2:2178751/100‑1 (MQ=255)
                          gCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAgg                                                                         <  2:530288/100‑1 (MQ=255)
                                aaTGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTac                                                                   <  2:2855990/100‑1 (MQ=255)
                                   gCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTAcaca                                                                >  2:1819132/1‑100 (MQ=255)
                                      gCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATg                                                             >  2:376540/1‑100 (MQ=255)
                                       cACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATga                                                            >  1:508982/1‑100 (MQ=255)
                                       cACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATga                                                            <  2:3022352/100‑1 (MQ=255)
                                          gACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGAc                                                         <  2:143991/100‑1 (MQ=255)
                                             tGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACaga                                                      >  2:2836772/1‑100 (MQ=255)
                                             tGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACaga                                                      >  1:1020552/1‑100 (MQ=255)
                                              ggATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaa                                                     >  1:3141072/1‑100 (MQ=255)
                                               naTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaag                                                    >  1:435064/2‑100 (MQ=255)
                                               gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaag                                                    <  1:1551759/100‑1 (MQ=255)
                                               gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaag                                                    <  1:289973/100‑1 (MQ=255)
                                               gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaag                                                    >  2:1398234/1‑100 (MQ=255)
                                                aTCTAGTAGATGCGCTGGCACGAGCGTCATTTTTGAGATAAAAATGCCCGCCGGTCGGGCTGGCATTTTGCCTTTAGGTTGTACACAATTAGAAagaaga                                                   <  1:1518792/100‑1 (MQ=255)
                                                aTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                   >  2:2214835/1‑100 (MQ=255)
                                                aTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                   <  2:150462/100‑1 (MQ=255)
                                                   cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCt                                                >  1:1043364/1‑100 (MQ=255)
                                                   cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCt                                                <  2:2507967/100‑1 (MQ=255)
                                                   cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCt                                                >  1:923303/1‑100 (MQ=255)
                                                   cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCt                                                <  1:998191/100‑1 (MQ=255)
                                                   cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCATTTACGATGTACACAATGAGACAGAAGAGCt                                                >  1:2133776/1‑100 (MQ=255)
                                                      tAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATg                                             <  1:1899025/100‑1 (MQ=255)
                                                       aGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATg                                             <  1:1898676/99‑1 (MQ=255)
                                                       aGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGc                                            <  1:1707054/100‑1 (MQ=255)
                                                       aGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGc                                            <  2:1711904/100‑1 (MQ=255)
                                                        gATGTTCTGGCACGAGAGTAATTTTTGAGATAAAAATGGCAGACGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATg                                             <  1:2316927/98‑1 (MQ=255)
                                                          tGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGAc                                         <  2:1814446/100‑1 (MQ=255)
                                                          tGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGAc                                         <  2:2805715/100‑1 (MQ=255)
                                                             tctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGc                                      >  1:1484187/1‑100 (MQ=255)
                                                                  cACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCtt                                 >  2:2083486/1‑100 (MQ=255)
                                                                    cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                               <  2:435066/100‑1 (MQ=255)
                                                                    cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                               <  1:507055/100‑1 (MQ=255)
                                                                    cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGAGCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                               >  1:3379970/1‑100 (MQ=255)
                                                                        agTAATTTTTGAGATACAAATGACAGACGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                               <  1:2059061/96‑1 (MQ=255)
                                                                        agTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACtt                           <  1:3116750/100‑1 (MQ=255)
                                                                         gTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTc                          <  2:2706804/100‑1 (MQ=255)
                                                                         gTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTc                          >  2:1967094/1‑100 (MQ=255)
                                                                          tAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCg                         >  1:2005992/1‑100 (MQ=255)
                                                                            aTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGAc                       <  1:3327088/100‑1 (MQ=255)
                                                                             tttttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACg                      <  1:845184/100‑1 (MQ=255)
                                                                                ttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGc                   <  1:1760668/100‑1 (MQ=255)
                                                                                ttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGc                   <  1:2861550/100‑1 (MQ=255)
                                                                                 tGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGca                  >  2:839382/1‑100 (MQ=255)
                                                                                 tGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGca                  <  2:1497922/100‑1 (MQ=255)
                                                                                  gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACTATGGGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGcac                 <  1:2633221/100‑1 (MQ=255)
                                                                                   agaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGcaca                >  2:954280/1‑100 (MQ=255)
                                                                                    aggcaaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACaa               >  2:3275410/5‑100 (MQ=38)
                                                                                     ggcaaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAAt              >  2:949604/4‑100 (MQ=38)
                                                                                     aTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAAt              >  1:65651/1‑100 (MQ=255)
                                                                                      tAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATa             <  1:1660551/100‑1 (MQ=255)
                                                                                       caaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa            >  2:3310344/2‑100 (MQ=38)
                                                                                       aaaaaTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa            >  2:3364433/1‑100 (MQ=255)
                                                                                       aaaaaTGCCAGCCGATCGGGCTGGCATTTGGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa            <  1:1667970/100‑1 (MQ=255)
                                                                                        aaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAac           >  1:840238/1‑100 (MQ=39)
                                                                                        aaaaTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAac           <  2:2384703/100‑1 (MQ=255)
                                                                                         aaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAaca          >  2:2836882/1‑100 (MQ=39)
                                                                                          aaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAacac         <  2:840241/100‑1 (MQ=39)
                                                                                          aaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAacac         >  2:265760/1‑100 (MQ=39)
                                                                                            tGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTg       >  2:261136/1‑100 (MQ=255)
                                                                                            tGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTg       >  2:1672642/1‑100 (MQ=255)
                                                                                             gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt        <  1:2607508/98‑1 (MQ=255)
                                                                                             gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg      <  1:306751/100‑1 (MQ=255)
                                                                                             gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg      >  2:829449/1‑100 (MQ=255)
                                                                                              ccAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGc     >  2:1026113/1‑100 (MQ=255)
                                                                                              ccAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGc     >  1:2753611/1‑100 (MQ=255)
                                                                                              ccAGCCGATCGGGCTGGCATTTGGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGc     >  2:2367042/1‑100 (MQ=255)
                                                                                               cAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCg    <  2:1088530/100‑1 (MQ=255)
                                                                                               cAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCg    >  2:1238557/1‑100 (MQ=255)
                                                                                                 gCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACCGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTg  <  1:2083473/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTG  >  NC_000913/922217‑922413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: