Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,357:1 +T 15.2% intergenic (+151/+21) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3571.T15.2% 354.0 / 65.2 105intergenic (+151/+21)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base . (45/44);  new base T (11/5);  total (56/49)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 110 total aligned reads displayed.

TTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA  >  NC_000913/1446259‑1446456
                                                                                                   |                                                                                                   
tttCCTGAAAAAAGGTGAATATTCAAAAACTCCTGTCAAATTGAAAAAAAAAAAAAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAg                                                                                                    <  1:2428211/100‑1 (MQ=255)
  tCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTAAAAAAAAAAAAAAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAg                                                                                                    <  1:666532/98‑1 (MQ=255)
   ccTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTAc                                                                                                 >  2:2190427/1‑100 (MQ=255)
    cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                >  1:102878/1‑100 (MQ=255)
    cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                >  2:306840/1‑100 (MQ=255)
    cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                >  2:1829983/1‑100 (MQ=255)
    cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                <  2:1547076/100‑1 (MQ=255)
    cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                >  1:133231/1‑100 (MQ=255)
     tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                               <  2:3313084/100‑1 (MQ=255)
     tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                               >  2:284253/1‑100 (MQ=255)
      gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTaaa                                                                                                >  2:1028749/1‑96 (MQ=255)
      gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                              >  1:1287957/1‑100 (MQ=255)
      gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                              <  1:3350022/100‑1 (MQ=255)
      gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGAGTTGCAGTACAt                                                                                               >  1:2329889/1‑99 (MQ=255)
      gAAACAAAGTGAATATTCAAAAACGCCTGTTTAATTTCCAAATGACAAAAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                              <  1:815560/100‑1 (MQ=255)
       aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                             <  1:2811767/100‑1 (MQ=255)
       aaaCAAGGTGAATATTCAAAAACTACTGTCAAATTGACTTTTGGCCTGAAAAAGGTTTAGTCAATTATCGGCTTTAACAAATGGGAGCTGCAGTAc                                                                                                 <  1:3184922/96‑1 (MQ=255)
        aaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCa                                                                                            <  1:1711213/100‑1 (MQ=255)
          cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                          >  2:2049225/1‑100 (MQ=255)
          cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                          <  1:2023874/100‑1 (MQ=255)
          cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                          <  1:1793026/100‑1 (MQ=255)
          cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                          <  2:475164/100‑1 (MQ=255)
            aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAACTATCTGCTTTAACTAATTGGCGTTGCAGTACATGCAAc                                                                                          <  1:2023840/98‑1 (MQ=255)
            aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGc                                                                                        <  1:883745/100‑1 (MQ=255)
             ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  2:2909581/100‑1 (MQ=38)
             ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  2:44181/100‑1 (MQ=38)
             ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  2:3156099/100‑1 (MQ=38)
             ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                       >  2:1504505/1‑100 (MQ=255)
             ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                       >  1:122065/1‑100 (MQ=255)
               tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCaa                                                                                     <  1:2006913/100‑1 (MQ=38)
               tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCaa                                                                                     >  2:1384607/1‑100 (MQ=255)
                gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAt                                                                                    >  1:2349861/1‑100 (MQ=255)
                 aaTATGAAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATATTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                   <  1:775854/100‑1 (MQ=255)
                  atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                  <  2:2498116/100‑1 (MQ=255)
                   tatTCAAAAACTCCTGTCAAATTGCCTTTTGCTCTGAAAAATGCATAGTCAAATATCTGTTTTACCTAATTGGCGTTGCAGTACATGCAACGCCAAttag                                                                                 >  1:2405353/1‑100 (MQ=255)
                   tatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAttag                                                                                 <  1:1557171/100‑1 (MQ=37)
                   tatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                  <  2:1185913/99‑1 (MQ=255)
                      tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtta                                                                              >  2:2104735/1‑100 (MQ=255)
                      tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                               <  1:930028/99‑1 (MQ=255)
                               ccTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAttt                                                                     >  1:249859/1‑100 (MQ=255)
                                 tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTAc                                                                   >  2:1423346/1‑100 (MQ=255)
                                 tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATGGGCGTTGCAGTACATACAACGCCAATTAGTTAGCGGAATTTa                                                                    >  1:1124319/1‑99 (MQ=255)
                                    cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                <  2:3081804/100‑1 (MQ=255)
                                        ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa                                                            <  2:1585041/100‑1 (MQ=255)
                                            cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                        >  2:1975943/1‑100 (MQ=255)
                                            cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                        <  2:2214669/100‑1 (MQ=255)
                                                tGCCCTAAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATATTCGCCTg                                                    <  1:2190412/100‑1 (MQ=255)
                                                 gCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCt                                                     >  1:2800587/1‑98 (MQ=255)
                                                  cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                  >  2:693447/1‑100 (MQ=255)
                                                   ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                 <  2:2758061/100‑1 (MQ=255)
                                                     tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTc                                               <  1:112089/100‑1 (MQ=255)
                                                       aaaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCAt                                             >  2:1176858/1‑100 (MQ=255)
                                                         aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATcc                                           <  2:946823/100‑1 (MQ=255)
                                                         aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATcc                                           <  1:3111010/100‑1 (MQ=255)
                                                          aaTGCATAGTCAAATATCTGTTTTAACTAAGTGGCGTTGCAGTACATGCAACGCCAAGTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATccc                                          <  2:1433410/100‑1 (MQ=255)
                                                              cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                      <  2:249860/100‑1 (MQ=255)
                                                               aTAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                     <  1:2593970/100‑1 (MQ=255)
                                                               aTAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                     <  1:2575412/100‑1 (MQ=255)
                                                                tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                    >  2:3308597/1‑100 (MQ=255)
                                                                tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                    >  2:38095/1‑100 (MQ=255)
                                                                   tCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTg                                 <  1:873748/100‑1 (MQ=255)
                                                                     aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCTAATTAGTTAGCGGAATTTACGTCGACACTCGCCTGGCGTCATCCCAAAGCGTTGc                                >  1:1369761/1‑99 (MQ=255)
                                                                     aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                               <  2:542856/100‑1 (MQ=255)
                                                                     aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                               <  1:3161147/100‑1 (MQ=255)
                                                                         atCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                           >  1:1637354/1‑100 (MQ=255)
                                                                         atCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                           <  2:2232354/100‑1 (MQ=255)
                                                                          tCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAAt                          <  2:1124322/100‑1 (MQ=255)
                                                                           cTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATa                         <  1:2452556/100‑1 (MQ=255)
                                                                            tGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                        >  2:2892153/1‑100 (MQ=255)
                                                                            tGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTAGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                        >  1:2697591/1‑100 (MQ=255)
                                                                             gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTGCGTCGATACGCGCCTGGAGTCATCCCAAAGCTGTACTTAAATAcc                       >  1:641682/1‑100 (MQ=38)
                                                                             gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                       >  2:1296187/1‑100 (MQ=255)
                                                                             gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                       >  1:1006338/1‑100 (MQ=255)
                                                                             gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTAATCCCAAAGCGTTGCTTAAATAcc                       >  1:2885213/1‑100 (MQ=255)
                                                                              ttttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAACTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg                      >  2:2178475/1‑100 (MQ=255)
                                                                              cgctAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg                      >  2:1561109/4‑100 (MQ=25)
                                                                               gctAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTAAGTAGGTAGCGCCCAGGGGTCTGCCCAAAGCGTTGCTTGAATACCGt                     >  1:1940196/3‑100 (MQ=9)
                                                                                ttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGtt                    >  2:1471294/1‑100 (MQ=255)
                                                                                 tAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                   <  1:3005980/100‑1 (MQ=255)
                                                                                     tAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaa               <  2:2020163/100‑1 (MQ=255)
                                                                                      aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa              >  2:2257339/1‑100 (MQ=255)
                                                                                       aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt             >  2:880485/1‑100 (MQ=38)
                                                                                       aTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaa               >  2:2983001/1‑98 (MQ=255)
                                                                                         tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa           >  2:265737/1‑100 (MQ=38)
                                                                                         tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa           >  1:3195099/1‑100 (MQ=38)
                                                                                         tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa           >  2:928287/1‑100 (MQ=38)
                                                                                          ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTAGGTCGATACTGGCCTGGCGTTATCCGAAAGCATGGCGTAAATAGCGTTGAAAAATGAc          >  1:831876/1‑100 (MQ=21)
                                                                                          ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:1100853/1‑100 (MQ=38)
                                                                                          ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  1:1467322/1‑100 (MQ=38)
                                                                                          ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  1:3315487/1‑100 (MQ=38)
                                                                                          ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          <  2:3074783/100‑1 (MQ=255)
                                                                                          ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:1880680/1‑100 (MQ=255)
                                                                                          ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:547864/1‑100 (MQ=255)
                                                                                          ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:2011679/1‑100 (MQ=255)
                                                                                               tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg     <  1:1874067/100‑1 (MQ=255)
                                                                                                gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt    <  2:360896/100‑1 (MQ=255)
                                                                                                 cAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCACCTGGCGTCATCCCAAAGCGTTGCTTAAATACCCTTGAAAAATGACTCTGGTc   >  1:168005/1‑100 (MQ=255)
                                                                                                  aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  >  1:3273894/1‑100 (MQ=255)
                                                                                                  aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  >  2:2049546/1‑100 (MQ=255)
                                                                                                  aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCGGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  >  1:999525/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA  >  NC_000913/1446259‑1446456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: