Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,665,195:1 (T)7→8 100% intergenic (+75/‑120) dmsD → / → clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,665,1881.T93.6% 250.6 / 10.3 78intergenic (+68/‑127)dmsD/clcBtwin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base T (28/45);  total (31/47)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑TTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCA  >  NC_000913/1665090‑1665286
                                                                                                    |                                                                                                    
tCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTTCAGTGTCACG‑‑t                                                                                                    <  1:1774592/100‑1 (MQ=255)
 ccTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑tt                                                                                                   >  1:3113172/1‑100 (MQ=255)
    gTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGCTTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑ttttt                                                                                                >  1:303091/1‑100 (MQ=255)
     tCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑tttttt                                                                                               <  2:2504211/100‑1 (MQ=255)
      cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑ttttttt                                                                                              >  1:105791/1‑100 (MQ=255)
      cgcgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑ttttttt                                                                                              >  1:275871/1‑100 (MQ=255)
        cgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑tttttttta                                                                                            <  2:1176612/100‑3 (MQ=255)
        cgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑tttttttta                                                                                            <  1:135759/100‑3 (MQ=255)
        cgGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑tttttttta                                                                                            <  2:2884793/100‑3 (MQ=255)
          gTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑ttttttttatc                                                                                          <  1:2122814/100‑5 (MQ=255)
          gTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑ttttttttatc                                                                                          <  2:2610387/100‑5 (MQ=255)
            tAAACCGCTGTTTCGATAGGATCACCGTAATATTGCTGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTAtctc                                                                                        >  2:2385216/1‑100 (MQ=255)
            tAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTAtctc                                                                                        <  2:1894319/100‑1 (MQ=255)
            tAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑tttttttt                                                                                             >  1:129896/1‑94 (MQ=255)
              aaCCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCtt                                                                                      >  2:3331871/1‑100 (MQ=255)
                ccGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTaa                                                                                    <  1:263216/100‑1 (MQ=255)
                ccGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTaa                                                                                    <  1:441011/100‑1 (MQ=255)
                ccGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTaa                                                                                    >  2:2467277/1‑100 (MQ=255)
                ccGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTaa                                                                                    >  2:2124998/1‑100 (MQ=255)
                  gCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAg                                                                                  <  2:2116740/100‑1 (MQ=255)
                   cTGTTTCGATAGGATCACGATAATATTGCCGGATGGTGATGATTACGCGCAATCCGGTATTAATATGTTACAGTGTCACGTTTTTTTTATCGCTTAAAg                                                                                  <  1:2137039/99‑1 (MQ=255)
                    tGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTCTCACGTTTTTTTTATCTCTTAAAgca                                                                                <  2:2057721/100‑1 (MQ=255)
                     gTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcac                                                                               <  1:529142/100‑1 (MQ=255)
                     gTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcac                                                                               >  2:489041/1‑100 (MQ=255)
                     acgctcttccgATCTCCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcac                                                                               <  1:119697/90‑1 (MQ=255)
                       ttCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcacgc                                                                             >  1:2603109/1‑100 (MQ=255)
                         cGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcacgcac                                                                           <  2:2023898/100‑1 (MQ=255)
                         cGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcacgcac                                                                           >  2:1537478/1‑100 (MQ=255)
                         cGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAgcacgcac                                                                           <  2:275872/100‑1 (MQ=255)
                          gtaagactCAACGTAACATTGCAGGATGGTGAAGATTAAGATAAATCTGACAATTATAGGTTACAATGTCACGTTTTTTTTATCTCTTAAAgcacgcac                                                                           <  1:1683093/92‑1 (MQ=255)
                             aGGTTCACCTTAAAATTGCCGGTTGGGGACGATTACGCGAAACCCGGCAATAATAGGTTACGGTGTCCCGTTTTTTTTATCTCTTAAAGCACGCACTGc                                                                        <  2:129896/99‑1 (MQ=255)
                              ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                      >  1:1570710/1‑100 (MQ=255)
                              ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                      <  2:1412652/100‑1 (MQ=255)
                              ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                      >  1:1328850/1‑100 (MQ=255)
                              ggATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                      <  2:2677764/100‑1 (MQ=255)
                              ggATCACCGTAATATGGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtt                                                                      <  2:545421/100‑1 (MQ=255)
                               gATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTACAGCACGCACTGCttt                                                                     >  1:1044083/1‑100 (MQ=255)
                                aTCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCtttt                                                                    <  2:3074204/100‑1 (MQ=255)
                                 tCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTg                                                                   <  2:1325506/100‑1 (MQ=255)
                                   aCCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGc                                                                  >  1:3150510/1‑99 (MQ=255)
                                   aCCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCg                                                                 <  2:429200/100‑1 (MQ=255)
                                   aCCGTAATATTACCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACATTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCg                                                                 <  2:2391142/100‑1 (MQ=255)
                                    ccGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg                                                                >  1:1177511/1‑100 (MQ=255)
                                        aaTATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTgg                                                            <  1:443032/100‑1 (MQ=255)
                                        aaTATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTgg                                                            <  1:1287192/100‑1 (MQ=255)
                                          tatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcc                                                          <  1:1355573/100‑1 (MQ=255)
                                           atTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCct                                                         >  2:2557477/1‑100 (MQ=255)
                                              gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtt                                                      <  1:635456/100‑1 (MQ=255)
                                               ccGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCttt                                                     <  2:2265007/100‑1 (MQ=255)
                                                cGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtttt                                                    <  1:3306824/100‑1 (MQ=255)
                                                    tGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGccn                                                <  2:2450351/100‑2 (MQ=255)
                                                        gACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGTACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaa                                            <  1:1809873/100‑1 (MQ=255)
                                                        gACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaa                                            >  2:1153753/1‑100 (MQ=255)
                                                        gACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaa                                            <  1:2467258/100‑1 (MQ=255)
                                                         aCGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaaa                                           >  2:723435/1‑100 (MQ=255)
                                                          cGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAAt                                          >  2:982788/1‑100 (MQ=255)
                                                           gATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATa                                         <  2:739250/100‑1 (MQ=255)
                                                             ttACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt                                       >  2:1359895/1‑100 (MQ=255)
                                                               aCGCGCAAGCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCg                                     <  2:753162/100‑1 (MQ=255)
                                                                cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc                                    >  2:2665969/1‑100 (MQ=255)
                                                                 gcgcAATCCGGCAATAATAGGTTACAGAGTCACGG‑‑TTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGct                                   >  2:2698936/1‑99 (MQ=255)
                                                                  cgcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGccc                                  <  1:1258309/100‑1 (MQ=255)
                                                                  cgcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGccc                                  >  2:1002694/1‑100 (MQ=255)
                                                                   gcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCg                                 <  2:3316540/100‑1 (MQ=255)
                                                                    ccaTCCGGCAATAATAGGTTACTGTGTCACGTTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGccc                                  <  2:234639/97‑1 (MQ=255)
                                                                      aTCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCgtgt                              >  2:1758799/1‑100 (MQ=255)
                                                                          ggCAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTTCCGCAAAATAGTCGCCCGTGTTTCa                          >  1:2659959/1‑100 (MQ=255)
                                                                            cAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAtt                        >  2:1232054/1‑100 (MQ=255)
                                                                            cAATAATAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAt                         >  1:798906/1‑99 (MQ=255)
                                                                             aataatAGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTg                       <  1:2455467/100‑1 (MQ=255)
                                                                              ataataGGTTACAGTGTCACGTTTTTTTTTTTTTTTTAAACACGCACTGCTCTTCCGGCTTGCCTCTTTTGCCGGAAACTAGTCGCCTGTGTTTCATTGc                      >  1:970495/1‑100 (MQ=255)
                                                                              ataataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGc                      <  2:2719725/100‑1 (MQ=255)
                                                                               taataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGTTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTggc                     <  2:2292685/100‑3 (MQ=255)
                                                                               taataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGcc                     <  2:2147920/100‑1 (MQ=255)
                                                                                 ataGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCa                   <  1:3331843/100‑1 (MQ=255)
                                                                                   aGGTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCAtt                 >  2:1818639/1‑100 (MQ=255)
                                                                                    ggTTACAGTGTCACG‑‑TTTTTTTAATCTCTTAAAGCACGCACTGCTTTTGCGGCTGACCTCTTTTGCCGAAAAATAGTCGCCCATGTTTAATTGcccct                  >  1:1215889/1‑96 (MQ=255)
                                                                                     gTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc               <  2:2484740/100‑1 (MQ=255)
                                                                                     gTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc               >  2:342743/1‑100 (MQ=255)
                                                                                     gTTACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc               <  2:2159019/100‑1 (MQ=255)
                                                                                       tACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg             >  2:1305370/1‑100 (MQ=255)
                                                                                       tACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg             >  2:84210/1‑100 (MQ=255)
                                                                                       tACAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg             <  2:963061/100‑1 (MQ=255)
                                                                                        aCAGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc            <  1:1286275/100‑1 (MQ=255)
                                                                                          aGTGTCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTc          <  1:668320/100‑1 (MQ=255)
                                                                                           gtgtCACG‑‑TTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCAt        >  1:1480660/1‑100 (MQ=255)
                                                                                            tgtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTc          <  2:2633432/98‑1 (MQ=255)
                                                                                            tgtCACG‑‑TTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg       <  2:1072197/100‑1 (MQ=255)
                                                                                             gtCACGTTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg       <  1:982785/100‑1 (MQ=255)
                                                                                                 cG‑‑TTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGcatca  <  1:2686858/100‑1 (MQ=255)
                                                                                                    |                                                                                                    
TCCTGTCGCGGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑‑TTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCA  >  NC_000913/1665090‑1665286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: