BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,946,790	T→G	60.1%	G180G (GGT→GGG)	yebB →	DUF830 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,790	0	T	G	60.1%	102.2 / 117.2	105	G180G (GGT→GGG)	yebB	DUF830 family protein
Reads supporting (aligned to +/- strand): ref base T (24/18); new base G (28/35); total (52/53)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

AATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAG  >  NC_000913/1946695‑1946884

aaTGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTgg                                                                                            <  1:831821/100‑1 (MQ=255)

  tGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGgtg                                                                                          <  2:2021168/100‑1 (MQ=255)

   gCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGgtgt                                                                                         <  2:754005/100‑1 (MQ=255)

    cAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGtt                                                                                        >  2:1642420/1‑100 (MQ=255)

    cAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGtt                                                                                        >  1:472950/1‑100 (MQ=255)

    cAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGtt                                                                                        <  1:48166/100‑1 (MQ=255)

    cAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGtt                                                                                        <  2:2303728/100‑1 (MQ=255)

      aaaCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGa                                                                                      <  1:2962730/100‑1 (MQ=255)

      aaaCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGa                                                                                      <  2:2171617/100‑1 (MQ=255)

       aaCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGa                                                                                      <  1:2324013/99‑1 (MQ=255)

       aaCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGa                                                                                      >  1:3050134/1‑99 (MQ=255)

        aCTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACTCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGAt                                                                                     <  1:1733309/99‑1 (MQ=255)

           cACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGAGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCAt                                                                                 >  1:1810939/1‑100 (MQ=255)

             cTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGc                                                                               >  1:899384/1‑100 (MQ=255)

               ttCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCgg                                                                             <  2:2653779/100‑1 (MQ=255)

                   ggAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCggaggg                                                                         >  1:1366834/1‑100 (MQ=255)

                   ggAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCggaggg                                                                         >  2:453407/1‑100 (MQ=255)

                   ggAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCggaggg                                                                         <  1:935433/100‑1 (MQ=255)

                   ggAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGATGGTGTTGATTCATGCggaggg                                                                         >  1:1683137/1‑100 (MQ=255)

                      aaTTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCAAGTTTGCGCCATCATCCGGGGTTGGTGTTGACTCCTGCGGTGGGAGt                                                                      >  1:973215/1‑100 (MQ=255)

                        ttCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTgg                                                                    >  2:690670/1‑100 (MQ=255)

                          cTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGaa                                                                  <  2:634675/100‑1 (MQ=255)

                                  tGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCa                                                          >  2:1187311/1‑100 (MQ=255)

                                   gggTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAg                                                         <  2:3050160/100‑1 (MQ=255)

                                   gggTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAg                                                         <  2:1062332/100‑1 (MQ=255)

                                   gggTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAg                                                         <  1:2264804/100‑1 (MQ=255)

                                     gTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGaaagatcggaaga                                                       >  2:2011749/1‑90 (MQ=255)

                                      ttCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCg                                                      <  2:3234862/100‑1 (MQ=255)

                                       tCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCgg                                                     >  1:2672419/1‑100 (MQ=255)

                                        cTATTGCGTGGGATCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGTAAACGCCTCAGCCGGa                                                    <  1:2713774/100‑1 (MQ=255)

                                        cTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGa                                                    <  2:2982043/100‑1 (MQ=255)

                                        cTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGa                                                    >  2:2964632/1‑100 (MQ=255)

                                        cTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGa                                                    >  1:2986698/1‑100 (MQ=255)

                                          aTTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATGATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAAc                                                  >  2:2384929/1‑100 (MQ=255)

                                          aTTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAAc                                                  >  1:3073469/1‑100 (MQ=255)

                                          aTTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAAc                                                  <  2:2823375/100‑1 (MQ=255)

                                          aTTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAAc                                                  <  1:840068/100‑1 (MQ=255)

                                           ttCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACt                                                 <  2:3329155/100‑1 (MQ=255)

                                           ttCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACt                                                 >  2:2903157/1‑100 (MQ=255)

                                            tCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTg                                                >  2:1809665/1‑100 (MQ=255)

                                              cGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGAc                                              >  2:2145336/1‑100 (MQ=255)

                                               gTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGAcc                                             <  1:3011396/100‑1 (MQ=255)

                                               gTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGAcc                                             <  1:2388193/100‑1 (MQ=255)

                                               gTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGAcc                                             <  1:2388113/100‑1 (MQ=255)

                                               gTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGAcc                                             >  1:1438314/1‑100 (MQ=255)

                                                tGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCg                                            >  1:1112310/1‑100 (MQ=255)

                                                   gAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAgg                                         >  1:1261721/1‑100 (MQ=255)

                                                    aGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGc                                        <  1:2491581/100‑1 (MQ=255)

                                                    aGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGc                                        >  1:1203489/1‑100 (MQ=255)

                                                      cGTAAACCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGCTTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGACCTGACCGAg                                          >  1:2797467/1‑96 (MQ=255)

                                                      cGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCgg                                      <  1:666724/100‑1 (MQ=255)

                                                      cGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCgg                                      >  1:232152/1‑100 (MQ=255)

                                                      cGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCgg                                      >  2:23911/1‑100 (MQ=255)

                                                      cGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCgg                                      <  1:3157698/100‑1 (MQ=255)

                                                      cGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCgg                                      <  2:1203493/100‑1 (MQ=255)

                                                       gTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGt                                     >  2:262734/1‑100 (MQ=255)

                                                       gTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGt                                     <  2:1056287/100‑1 (MQ=255)

                                                        tAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGta                                    <  2:1186834/100‑1 (MQ=255)

                                                        tAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGta                                    >  1:2544655/1‑100 (MQ=255)

                                                         aaaaCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGtat                                   >  1:2537615/1‑100 (MQ=255)

                                                         aaaaCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGtat                                   >  2:2042718/1‑100 (MQ=255)

                                                            aCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAAc                                <  2:1102414/100‑1 (MQ=255)

                                                             ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACt                               <  2:3138950/100‑1 (MQ=255)

                                                             ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGCGGGAGTGGAAACGCCCCAGCCGGAACTGACCGAGGCGGTATAACt                               >  1:3062307/1‑100 (MQ=255)

                                                             ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACt                               <  1:3147422/100‑1 (MQ=255)

                                                             ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACt                               <  1:1598096/100‑1 (MQ=255)

                                                             ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACt                               <  2:3274647/100‑1 (MQ=255)

                                                              cGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACtt                              <  2:2528018/100‑1 (MQ=255)

                                                               gTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTa                             >  2:1734909/1‑100 (MQ=255)

                                                               gTCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTa                             <  1:2077294/100‑1 (MQ=255)

                                                                tCACGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTaa                            <  2:2837526/100‑1 (MQ=255)

                                                                  ccGCCAGCCAGTTTCTGGCATCACCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTTGAACCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACg                          <  1:1797002/99‑1 (MQ=255)

                                                                  aCGCCAGCCAGTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACg                          <  1:1571444/100‑1 (MQ=255)

                                                                     ccagccagTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAg                       <  1:2212161/100‑1 (MQ=255)

                                                                      cagccagTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAg                       >  1:345849/1‑99 (MQ=255)

                                                                      cagccagTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGt                      >  2:2312781/1‑100 (MQ=255)

                                                                      cagccagTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGt                      >  2:2314477/1‑100 (MQ=255)

                                                                       agccagTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTc                     <  2:1468035/100‑1 (MQ=255)

                                                                       agccagTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTc                     >  1:1170964/1‑100 (MQ=255)

                                                                       agccagTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTc                     >  1:1170916/1‑100 (MQ=255)

                                                                         ccagTTTGTGGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGCGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGc                   >  2:214834/1‑100 (MQ=255)

                                                                          cagTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGcc                  >  2:1557572/1‑100 (MQ=255)

                                                                            gTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCct                >  2:2462453/1‑100 (MQ=255)

                                                                             tttGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCctc               <  2:175712/100‑1 (MQ=255)

                                                                                gtgtCATCATCCGGGGTTGTTGTTGATTCATGCGGAATGAGTGGATACGCCTCAGCCGCAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTTTCg            <  1:102214/100‑1 (MQ=255)

                                                                                 tgGCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGc           >  2:1753089/1‑100 (MQ=255)

                                                                                  ggCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTTGCCCTCTCGcc          >  1:2783/1‑100 (MQ=255)

                                                                                  ggCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGcc          >  2:2120187/1‑100 (MQ=255)

                                                                                  ggCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGcc          >  2:728957/1‑100 (MQ=255)

                                                                                   gCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCa         >  1:2658267/1‑100 (MQ=255)

                                                                                   gCATCATCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCa         >  2:2447388/1‑100 (MQ=255)

                                                                                    catcatCCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAg        <  2:622455/100‑1 (MQ=255)

                                                                                    catcagCCGGGGTGGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAg        <  1:2569895/100‑1 (MQ=255)

                                                                                     atcatcCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAgg       <  2:1190042/100‑1 (MQ=255)

                                                                                       catcCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGtt     <  2:3190499/100‑1 (MQ=255)

                                                                                        atcCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc    >  2:1286349/1‑100 (MQ=255)

                                                                                        atcCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc    >  2:1286288/1‑100 (MQ=255)

                                                                                         tcCGGGGTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCa   <  2:3112699/100‑1 (MQ=255)

                                                                                          ccGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAg  <  1:1440712/100‑1 (MQ=255)

                                                                                          ccGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACACAGTCGCCCTCTCGCCAGGTTCa   <  1:483597/99‑1 (MQ=255)

AATGCAAAACTCACTTTCTGGAAATTCTGGTTCCTGGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAG  >  NC_000913/1946695‑1946884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`