Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,971,761 T→C 57.8% T311A (ACC→GCC)  tar ← methyl‑accepting chemotaxis protein II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,971,7610TC57.8% 58.0 / 116.5 102T311A (ACC→GCC) tarmethyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base T (22/21);  new base C (29/30);  total (51/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 102 total aligned reads displayed.

TCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAC  >  NC_000913/1971664‑1971859
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tCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGa                                                                                                  <  2:2866597/100‑1 (MQ=255)
 ccaccaCTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAg                                                                                                 >  2:2672426/1‑100 (MQ=255)
  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgc                                                                                                <  1:2046818/100‑1 (MQ=255)
  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgc                                                                                                >  1:3310575/1‑100 (MQ=255)
  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgc                                                                                                >  1:834343/1‑100 (MQ=255)
  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgc                                                                                                <  1:1399094/100‑1 (MQ=255)
    ccacATTGCCGGCGTGCTGGGCGGTGTCGGAGGCACTTTGGGCCAGTTGCGAGGCCTGGCGGGCGTGATCGGCGTTTTGCTTCACTGTCGCGGCGAgc                                                                                                <  1:2883165/98‑1 (MQ=255)
      acTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgctgct                                                                                            <  1:2435416/100‑1 (MQ=255)
        tttGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTcc                                                                                          >  1:528507/1‑100 (MQ=255)
        tttGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTcc                                                                                          <  1:129174/100‑1 (MQ=255)
          tGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCAt                                                                                        >  2:1979157/1‑100 (MQ=255)
            ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGc                                                                                      <  2:1049239/100‑1 (MQ=255)
            ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                      <  1:151678/100‑1 (MQ=255)
            ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                      >  1:3357544/1‑100 (MQ=255)
            ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTGTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                      >  1:159678/1‑100 (MQ=255)
             cgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCt                                                                                     >  2:1970677/1‑100 (MQ=255)
             cgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                      <  2:776056/99‑1 (MQ=255)
                cgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggc                                                                                  <  2:1914670/100‑1 (MQ=255)
                   tcTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggcggc                                                                               <  1:2559704/99‑1 (MQ=255)
                   gCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTGGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcggc                                                                               <  1:750075/100‑1 (MQ=255)
                     tGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAg                                                                             <  1:2655696/100‑1 (MQ=255)
                       ggCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGtt                                                                           >  2:3060090/1‑100 (MQ=255)
                         cGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttc                                                                         <  1:195685/100‑1 (MQ=255)
                          ggTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttct                                                                        >  1:2313067/1‑100 (MQ=255)
                          ggTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttct                                                                        >  1:3264788/1‑100 (MQ=255)
                           gtgtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTCTTGCTTCACAGTCGCAGTGAGCTGGTCCATGCTGGCGGCAGTttctt                                                                       >  1:2289979/1‑100 (MQ=38)
                            tgtCGGGGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttctt                                                                       <  1:707847/99‑1 (MQ=255)
                             gtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTcc                                                                     <  1:2457011/100‑1 (MQ=255)
                              tCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGCCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGGGCCATGCTGGCGGCAGTTTCTTCCa                                                                    >  1:1123643/1‑100 (MQ=38)
                               cGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAg                                                                   >  1:586031/1‑100 (MQ=255)
                               cGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAg                                                                   <  2:1453567/100‑1 (MQ=255)
                               cGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAg                                                                   <  2:2449878/100‑1 (MQ=255)
                               cGGACGCCCTTTGCGCCAGTAGCGAGGACTAACCGGCGTTATCGGCGTTTTGCTTAACTGTCGCGCCGAGCTGCTCCATGCTGGCGGCAGTTTCATCCAg                                                                   >  1:1386674/1‑100 (MQ=25)
                                    gCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCgg                                                              >  1:3081887/1‑100 (MQ=255)
                                     cACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGa                                                             >  2:3124340/1‑100 (MQ=255)
                                     cACTTTGCGCCAGTTGCGAGCCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGa                                                             >  1:2063594/1‑100 (MQ=255)
                                      aCTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGAt                                                            >  2:444991/1‑100 (MQ=255)
                                       cTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATg                                                           >  1:2341668/1‑100 (MQ=255)
                                       cTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGGAGTTTCTTCCAGCGTGGAt                                                            >  1:3240900/1‑99 (MQ=255)
                                       cTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATg                                                           >  2:433395/1‑100 (MQ=255)
                                         ttGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGcc                                                         <  2:2797222/100‑1 (MQ=255)
                                         ttGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGcc                                                         <  1:928593/100‑1 (MQ=255)
                                           gcgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctg                                                       >  1:2447602/1‑100 (MQ=255)
                                           gcgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctg                                                       <  2:3362748/100‑1 (MQ=255)
                                            cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                      >  2:2021014/1‑100 (MQ=255)
                                            cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                      <  2:3176403/100‑1 (MQ=255)
                                              ccAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgctg                                                    <  2:1442549/100‑1 (MQ=255)
                                              ccAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgctg                                                    <  1:711909/100‑1 (MQ=255)
                                               cAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGt                                                   >  1:978677/1‑100 (MQ=255)
                                                 gTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTc                                                 <  1:2671026/100‑1 (MQ=255)
                                                   tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg                                               >  2:245210/1‑100 (MQ=255)
                                                   tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCaa                                               <  2:1861529/100‑2 (MQ=255)
                                                   tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg                                               >  1:300828/1‑100 (MQ=255)
                                                    gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGCTGCCTGCTGTTCAGt                                              >  1:592411/1‑100 (MQ=255)
                                                    gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt                                              >  2:2463818/1‑100 (MQ=255)
                                                    gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt                                              >  2:1425794/1‑100 (MQ=255)
                                                     cGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTa                                             >  1:3085806/1‑100 (MQ=255)
                                                     cGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTa                                             >  1:567911/1‑100 (MQ=255)
                                                       aGGCCTAGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACg                                           <  2:2781643/100‑1 (MQ=255)
                                                        ggCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACgg                                          >  2:1785911/1‑100 (MQ=255)
                                                         gCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACggg                                         <  1:3275662/100‑1 (MQ=255)
                                                          ncTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGgga                                        >  2:2002947/2‑100 (MQ=255)
                                                           cTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggag                                       <  2:2313084/100‑1 (MQ=255)
                                                           cTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggag                                       >  1:1136129/1‑100 (MQ=255)
                                                           cTGGCGCGCCTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATTCCTGCTGTTCAGTACGggag                                       <  1:64613/100‑1 (MQ=255)
                                                            tGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCCTCCAGCGCGGATGCCTGCTGTTCAGTACGggagg                                      >  1:1777888/1‑100 (MQ=255)
                                                               cgggcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGaaa                                   <  1:3060064/100‑1 (MQ=255)
                                                                gggcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAg                                  <  2:1133259/100‑1 (MQ=255)
                                                                gggcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAg                                  <  2:320731/100‑1 (MQ=255)
                                                                   cgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                               <  1:2723114/100‑1 (MQ=255)
                                                                   cgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                               <  2:325508/100‑1 (MQ=255)
                                                                   cgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                               <  2:3098897/100‑1 (MQ=255)
                                                                     ttATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTACAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCgg                             >  2:1583024/1‑100 (MQ=255)
                                                                       aTCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtg                           >  2:269402/1‑100 (MQ=255)
                                                                       aTCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtg                           >  2:269475/1‑100 (MQ=255)
                                                                       aTCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtg                           <  2:2770798/100‑1 (MQ=255)
                                                                         cGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGtt                         >  2:919154/1‑100 (MQ=255)
                                                                           gCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTGAGTACGGGAGGAAAGATCGGTGTTGc                       <  1:3244736/100‑1 (MQ=255)
                                                                           gCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGc                       <  1:3153764/100‑1 (MQ=255)
                                                                                ttGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgcc                  <  2:1266533/100‑1 (MQ=255)
                                                                                 tGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgccg                 >  1:1669586/1‑100 (MQ=255)
                                                                                 tGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgccg                 <  2:1678730/100‑1 (MQ=255)
                                                                                 tGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgccg                 <  2:1136132/100‑1 (MQ=255)
                                                                                  gCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc                <  1:1360337/100‑1 (MQ=255)
                                                                                    ttCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCaa              >  2:2764496/1‑100 (MQ=255)
                                                                                    ttCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCaa              >  1:542896/1‑100 (MQ=255)
                                                                                     tCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAt             <  2:432072/100‑1 (MQ=255)
                                                                                     tCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAt             >  2:1536013/1‑100 (MQ=255)
                                                                                      cACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAtt            <  1:2067741/100‑1 (MQ=255)
                                                                                       aCTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAttt           <  2:53442/100‑1 (MQ=255)
                                                                                        cTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTc          >  1:1051544/1‑100 (MQ=255)
                                                                                          gTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCAc        >  2:2097447/1‑100 (MQ=255)
                                                                                            cgcgGTGAGCTGCTCCATGCTGGCGGCATGTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg      <  2:2447621/100‑1 (MQ=255)
                                                                                            cgcgGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg      <  2:542899/100‑1 (MQ=255)
                                                                                            cgcgGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg      <  1:474087/100‑1 (MQ=255)
                                                                                            cgcgGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg      <  1:2441193/100‑1 (MQ=255)
                                                                                             gcgGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACggg     >  2:277682/1‑100 (MQ=255)
                                                                                               ggTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTa   >  1:3307217/1‑100 (MQ=255)
                                                                                               ggTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTa   >  2:3249348/1‑100 (MQ=255)
                                                                                                gTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAc  <  1:1275277/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
TCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAC  >  NC_000913/1971664‑1971859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: