Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,983,761 C→T 100% E437K (GAA→AAA)  araG ← L‑arabinose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,983,7610CT99.0% 336.4 / ‑4.5 100E437K (GAA→AAA) araGL‑arabinose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (69/30);  total (69/31)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 101 total aligned reads displayed.

CCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCTG  >  NC_000913/1983666‑1983857
                                                                                               |                                                                                                
ccGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGc                                                                                              >  1:2211451/1‑100 (MQ=255)
 cGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCt                                                                                             >  1:1823461/1‑100 (MQ=255)
   gTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTa                                                                                           <  1:2203147/100‑1 (MQ=255)
   gTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTa                                                                                           >  2:33106/1‑100 (MQ=255)
      ggCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcg                                                                                        >  1:523042/1‑100 (MQ=255)
      ggCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcg                                                                                        >  2:1897487/1‑100 (MQ=255)
       ggaaCGCCGAGGAATTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc                                                                                       >  2:2283025/3‑100 (MQ=255)
        cAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGcc                                                                                      >  1:2273448/1‑100 (MQ=255)
        cAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGcc                                                                                      <  2:523045/100‑1 (MQ=255)
        cAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGcc                                                                                      >  2:2672819/1‑100 (MQ=255)
          aCGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCaa                                                                                    <  1:1594984/100‑1 (MQ=255)
          aCGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCaa                                                                                    >  2:2496368/1‑100 (MQ=255)
             ccGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACAt                                                                                 >  2:2576756/1‑100 (MQ=255)
             ccGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACAt                                                                                 >  1:890749/1‑100 (MQ=255)
             ccGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACAt                                                                                 >  1:2629034/1‑100 (MQ=255)
               taGGACTGCAGGCAAGTCGCGGGAGGCAAATAGCACTGATACGCCGTGCGCCGCCAGGGCATAAATGACGTTATATATTTTGTGCTTAGCGCCAAc                                                                                   >  1:400718/2‑96 (MQ=255)
                aGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCaa                                                                              <  1:1857880/100‑1 (MQ=255)
                    cTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATCTTTTGTGCTTAGCGCCAACATCAATGcc                                                                          >  1:300737/1‑100 (MQ=255)
                    cTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGcc                                                                          >  2:3166617/1‑100 (MQ=255)
                     ttCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcg                                                                         <  1:410623/100‑1 (MQ=255)
                      tCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgc                                                                        >  2:1458095/1‑100 (MQ=255)
                         ggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcgcg                                                                     >  2:1204285/1‑100 (MQ=255)
                         ggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcgcg                                                                     >  2:3208813/1‑100 (MQ=255)
                          gTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGt                                                                    >  2:412519/1‑100 (MQ=255)
                           tAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCCACGCCGCGCGTa                                                                   <  2:1471435/100‑1 (MQ=255)
                            aaGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAg                                                                  >  2:2004857/1‑100 (MQ=255)
                              gTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGt                                                                >  2:1307103/1‑100 (MQ=255)
                              gTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGATATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGt                                                                >  2:2871368/1‑100 (MQ=255)
                                cGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTc                                                              >  2:2107959/1‑100 (MQ=255)
                                 gCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCa                                                             >  1:3008895/1‑100 (MQ=255)
                                 gCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCa                                                             >  1:1786701/1‑100 (MQ=255)
                                  cTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCAt                                                            >  2:2342507/1‑100 (MQ=255)
                                  cTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCAt                                                            >  2:102668/1‑100 (MQ=255)
                                  cTGGAGGCAAACAGCACCGCCACGCCCTGAGCCGCCAGCGCATAAATTAGGGTATATATTTTGTACTTAGCGCCAACATCAATGCCGCGCGTAGGt                                                                >  1:1241/1‑96 (MQ=255)
                                    ggAGGCAAACAGCATCGCCACGCCCTGCGTCGCCAGCGCATAAATTACGTTATATATTTTGTTCTTAGAGCCAACATCAATTCCGCTCGTAGGTTCAt                                                            >  1:1590847/1‑98 (MQ=255)
                                    ggAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATcc                                                          >  1:2953634/1‑100 (MQ=255)
                                    ggAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATcc                                                          <  2:2963701/100‑1 (MQ=255)
                                     gAGGCAAACCGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCa                                                         <  1:1247497/100‑1 (MQ=255)
                                      aGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCa                                                         >  2:2326377/1‑99 (MQ=255)
                                       ggCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGc                                                       >  1:2370775/1‑100 (MQ=255)
                                       ggCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGc                                                       >  2:359596/1‑100 (MQ=255)
                                        gCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCa                                                      >  2:415462/1‑100 (MQ=255)
                                         cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                     >  2:1259659/1‑100 (MQ=255)
                                         cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                     >  1:923967/1‑100 (MQ=255)
                                           aaCAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaaaa                                                   >  1:694340/1‑100 (MQ=255)
                                              aGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGa                                                <  2:2489470/100‑1 (MQ=255)
                                               gCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAc                                               >  1:873890/1‑100 (MQ=255)
                                               gCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTCATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAc                                               >  1:546214/1‑100 (MQ=255)
                                                cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAcc                                              >  1:1961074/1‑100 (MQ=255)
                                                 aCCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCt                                             >  1:1799435/1‑100 (MQ=255)
                                                 aCCGCCACGCCCTGCGCCACCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCt                                             <  2:2959150/100‑1 (MQ=255)
                                                  ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                            >  2:3317631/1‑100 (MQ=255)
                                                  ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                            >  1:273110/1‑100 (MQ=255)
                                                  ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                            >  2:1473396/1‑100 (MQ=255)
                                                      cACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtc                                        <  2:833561/100‑1 (MQ=255)
                                                      cACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtc                                        <  1:2125236/100‑1 (MQ=255)
                                                          cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCtt                                    >  1:164565/1‑100 (MQ=255)
                                                          cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCtt                                    <  2:2151198/100‑1 (MQ=255)
                                                          cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCtt                                    >  2:1263069/1‑100 (MQ=255)
                                                          cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCtt                                    >  2:2123050/1‑100 (MQ=255)
                                                            cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                  <  1:329730/100‑1 (MQ=255)
                                                            cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                  <  1:2416616/100‑1 (MQ=255)
                                                             tGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCg                                 >  2:942783/1‑100 (MQ=255)
                                                               cgccgcCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGAt                               <  1:2323600/100‑1 (MQ=255)
                                                                   gccAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAAcc                           >  1:1913232/1‑100 (MQ=255)
                                                                      aGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGc                        <  2:873893/100‑1 (MQ=255)
                                                                      aGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGc                        <  2:1059925/100‑1 (MQ=255)
                                                                        cgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCgg                      >  1:2416059/1‑100 (MQ=255)
                                                                        cgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCgg                      >  2:336348/1‑100 (MQ=255)
                                                                        cgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCg                       >  2:336572/1‑99 (MQ=255)
                                                                        cgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAAACAGCg                       >  1:2291263/1‑99 (MQ=255)
                                                                        cgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTATTCCGATAACCAGCgg                      >  1:1983051/1‑100 (MQ=255)
                                                                         gcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGc                     >  1:3070832/1‑100 (MQ=255)
                                                                          cATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGcc                    <  2:763977/100‑1 (MQ=255)
                                                                          cATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGcc                    >  1:1485303/1‑100 (MQ=255)
                                                                            tAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCa                  >  1:2118505/1‑100 (MQ=255)
                                                                            tAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCa                  >  2:2945386/1‑100 (MQ=255)
                                                                               aTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTAATCCAGCAAAATGACCTTCATCTCATCCGATAACCAGCGGCCCAGaa               >  1:2292047/1‑100 (MQ=255)
                                                                                ttACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAAt              <  1:2483672/100‑1 (MQ=255)
                                                                                ttACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCACAGAAt              >  1:1505432/1‑100 (MQ=255)
                                                                                 tACGTTATACATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATg             <  1:210147/100‑1 (MQ=255)
                                                                                   cGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGc           <  1:2007798/100‑1 (MQ=255)
                                                                                    gTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCt          >  1:1634457/1‑100 (MQ=255)
                                                                                    gTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCt          >  1:530983/1‑100 (MQ=255)
                                                                                    gTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCt          >  1:2106679/1‑100 (MQ=255)
                                                                                    gTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCt          <  2:2017010/100‑1 (MQ=255)
                                                                                      tatataTTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttt        >  2:2768543/1‑100 (MQ=255)
                                                                                        tatgtTTTGTGCTTAGCGCCCACATCAATGCCGCGCGTACGTTCATCCGGCAACATGACCTTCCTCTCCTCCGATATCCAGCGGCCCAGCAGGGCttt        >  1:1896018/1‑98 (MQ=255)
                                                                                        tatatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttttt      >  1:1765751/1‑100 (MQ=255)
                                                                                        tatatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttttt      <  1:3315370/100‑1 (MQ=255)
                                                                                        tatatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttttt      <  2:1188511/100‑1 (MQ=255)
                                                                                        natatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGACttttt      >  2:422130/2‑100 (MQ=255)
                                                                                         atatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTg     <  1:1363431/100‑1 (MQ=255)
                                                                                         atatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTg     >  1:469743/1‑100 (MQ=255)
                                                                                          tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc    <  2:1634464/100‑1 (MQ=255)
                                                                                           atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt   <  1:98884/100‑1 (MQ=255)
                                                                                           atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt   <  1:1263065/100‑1 (MQ=255)
                                                                                           atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt   <  2:741749/100‑1 (MQ=255)
                                                                                           atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt   <  2:2133436/100‑1 (MQ=255)
                                                                                            ttttGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCTg  >  1:186731/1‑100 (MQ=255)
                                                                                               |                                                                                                
CCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCTG  >  NC_000913/1983666‑1983857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: