Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,158 G→C 15.3% intergenic (‑141/+30) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,1580GC15.3% 333.0 / 49.1 118intergenic (‑141/+30)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (49/51);  new base C (7/11);  total (56/62)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 126 total aligned reads displayed.

CCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTG  >  NC_000913/2028059‑2028257
                                                                                                   |                                                                                                   
ccTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtg                                                                                                     >  2:53420/1‑100 (MQ=255)
ccTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtg                                                                                                     <  2:2016825/100‑1 (MQ=255)
ccTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtg                                                                                                     >  2:183263/1‑100 (MQ=255)
ccTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtg                                                                                                     >  1:2981594/1‑100 (MQ=255)
ccTGGCGATAGCGTACCGCAAAACAGCGATAATGTTATAATGGTGGCATGATAAGACGCGACTATCATTCGTTGCAGGCATTAGCACACCAGGCCGGAtg                                                                                                     >  1:2043235/1‑100 (MQ=255)
    gCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt                                                                                                 <  2:3350418/100‑1 (MQ=255)
     cGATAGCGTACCGCAAAACAGAAATAATTTTATAATGGTGGCATGATAAGACGCAACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt                                                                                                 >  1:1233364/1‑99 (MQ=255)
       aTAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCg                                                                                              <  2:2894759/100‑1 (MQ=255)
        tAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg                                                                                             <  2:2166184/100‑1 (MQ=255)
        tAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg                                                                                             <  1:1592626/100‑1 (MQ=255)
         aGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                            >  2:2929009/1‑100 (MQ=255)
           cGTACCGTAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCt                                                                                          <  1:1045430/100‑1 (MQ=255)
           cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                          <  1:943584/100‑1 (MQ=255)
            gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTg                                                                                         >  1:332264/1‑100 (MQ=255)
            gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTg                                                                                         <  2:1755241/100‑1 (MQ=255)
            gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                         <  2:406834/100‑1 (MQ=255)
             tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGa                                                                                        >  1:157405/1‑100 (MQ=255)
             tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGa                                                                                        <  2:293149/100‑1 (MQ=255)
             tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTgg                                                                                        <  2:1093398/100‑2 (MQ=255)
              aCCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGAt                                                                                       <  1:2488893/100‑1 (MQ=255)
               ccGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                      >  2:1911576/1‑100 (MQ=255)
               ccGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                      >  2:238469/1‑100 (MQ=255)
                cGCACAACAGCGATAATTTCATAAAGGAGGCACCATAAGACGCCACTATCATTCATTACAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCCGATg                                                                                      >  1:1498744/1‑99 (MQ=255)
                cGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt                                                                                     <  1:307424/100‑1 (MQ=255)
                cGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt                                                                                     >  1:2231290/1‑100 (MQ=255)
                cGCAAAACAGCGATAATTTGAGAATGGTGGCATGTTAAGACGAGACTAACATCCACCGCCGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                          <  1:170746/95‑1 (MQ=255)
                 gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtg                                                                                    <  2:2277861/100‑2 (MQ=37)
                 gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtg                                                                                    <  1:167463/100‑2 (MQ=37)
                 gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtg                                                                                    <  1:2900003/100‑2 (MQ=37)
                 gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGtt                                                                                    <  2:1058864/100‑1 (MQ=255)
                 gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt                                                                                     >  1:1779269/1‑99 (MQ=255)
                     aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                <  1:984164/100‑1 (MQ=255)
                     aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                <  2:157405/100‑1 (MQ=255)
                     aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                <  2:181783/100‑1 (MQ=255)
                        aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtgctaatgc                                                                             <  1:1831067/100‑9 (MQ=21)
                        aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATa                                                                             >  2:2127605/1‑100 (MQ=255)
                         gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtgctaatgcc                                                                            <  2:2758845/100‑10 (MQ=21)
                                 tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac                                                                    <  1:3247160/100‑1 (MQ=255)
                                 tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac                                                                    <  2:1649609/100‑1 (MQ=255)
                                 tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac                                                                    <  1:342987/100‑1 (MQ=255)
                                 tttAGAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac                                                                    <  2:3241251/100‑1 (MQ=255)
                                  ttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcaca                                                                   >  2:2623573/1‑100 (MQ=255)
                                    ataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACAct                                                                 <  2:1234079/100‑1 (MQ=255)
                                     taATGGTGGCATGATAAGTCGCGACTATCATTCATTGCAGGCATTAGCACACTAGGCCGGACGTTATCCGGCCTGATGTTAATGATACCCTTTCACAgtt                                                                >  1:10472/1‑100 (MQ=255)
                                     taATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActt                                                                >  2:1869137/1‑100 (MQ=255)
                                     cttccgatctaTGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActt                                                                <  2:590296/90‑1 (MQ=255)
                                        tggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtctt                                                             <  1:1927745/100‑1 (MQ=255)
                                         ggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACttcttc                                                            <  2:533325/100‑1 (MQ=255)
                                          gtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                           <  2:3141429/100‑1 (MQ=255)
                                           tggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCg                                                          <  2:572782/100‑1 (MQ=255)
                                            ggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                         >  2:1346905/1‑100 (MQ=255)
                                              cATGATAAGACGAGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCa                                                        >  1:2309026/1‑99 (MQ=255)
                                               aTGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGa                                                      <  2:3153065/100‑1 (MQ=255)
                                                tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGaa                                                     >  2:1969803/1‑100 (MQ=255)
                                                 gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAAc                                                    <  2:2990944/100‑1 (MQ=255)
                                                  aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACg                                                   <  2:443175/100‑1 (MQ=255)
                                                  aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACg                                                   <  1:3283231/100‑1 (MQ=255)
                                                  aTAAGACGCGACTATCATTCATCGCAGGCATCAGCACCCCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACg                                                   <  1:2282310/100‑1 (MQ=255)
                                                   tAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGa                                                  >  2:2150774/1‑100 (MQ=255)
                                                     aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                                <  1:1730999/100‑1 (MQ=255)
                                                     aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                                >  2:2720750/1‑100 (MQ=255)
                                                     aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                                <  1:3240172/100‑1 (MQ=255)
                                                         gcgACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccc                                            <  1:2424922/100‑1 (MQ=255)
                                                          cgACTATCATTCATTGCAGGAATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccct                                           >  1:2067376/1‑100 (MQ=255)
                                                             cTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                        >  1:2505430/1‑100 (MQ=255)
                                                              tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACtcccctcccc                                       >  2:603716/1‑100 (MQ=255)
                                                                  attcattGCAGGCATTAGCCCACCAGGCAGGAGGTGATCCGGCCTGATGTTAATGATACCCTTTCAGAATACTTCCGGAGAACGACtgccctcccc                                       >  1:1273590/1‑96 (MQ=255)
                                                                  attcattGCAGGCATTAGCACACCGGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGa                                   <  1:2699170/100‑1 (MQ=255)
                                                                  attcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCgg                                    >  1:1771293/1‑99 (MQ=255)
                                                                    tcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGTCCTGATGTTAATGATCTCCTTTCACACGTCTTCCGCAGAACGACTCCCCTCCCCCGGa                                   <  1:1852333/98‑1 (MQ=255)
                                                                    tcattGCAGGCATTAGCACACCAGGCCGCATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACCCCCCTCCCCCGGAAc                                 <  1:2640664/100‑1 (MQ=255)
                                                                     cattGCAGGCATTAGCACACCAGGCCGGATGTGATACGGCCTGATGTTAATGATACCCATTCACACTTATTCCGCAGAACGACTCCACTCCCCCGGAACa                                >  1:3374403/1‑100 (MQ=255)
                                                                      attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGGTAATGATACCCTTTTACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACaa                               >  1:2387173/1‑100 (MQ=255)
                                                                         gCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCt                            >  1:11151/1‑100 (MQ=255)
                                                                         gCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCt                            >  2:781244/1‑100 (MQ=255)
                                                                          cAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                           >  1:2667325/1‑100 (MQ=255)
                                                                            ggCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCgg                         >  2:194233/1‑100 (MQ=255)
                                                                                 tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                    >  1:1174978/1‑100 (MQ=255)
                                                                                 attaacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGCAACAAGCTCGGTTTCa                    >  1:2091151/5‑100 (MQ=25)
                                                                                  aGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                   >  2:1527683/1‑100 (MQ=255)
                                                                                   gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                   >  1:887000/1‑99 (MQ=255)
                                                                                   gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                  >  2:236684/1‑100 (MQ=255)
                                                                                   gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                  <  1:603713/100‑1 (MQ=255)
                                                                                   gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                  <  1:1454779/100‑1 (MQ=255)
                                                                                      cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa               <  1:842285/100‑1 (MQ=255)
                                                                                      cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa               >  2:2015189/1‑100 (MQ=255)
                                                                                       acCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaaa              >  2:3339532/1‑100 (MQ=255)
                                                                                        ccAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAg             >  2:302109/1‑100 (MQ=255)
                                                                                         cAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc            >  2:1243817/1‑100 (MQ=255)
                                                                                         cAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc            <  2:2055713/100‑1 (MQ=255)
                                                                                           ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCtt          >  2:396673/1‑100 (MQ=255)
                                                                                           ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGCAACAAGCTCGGTTTCATGAAAAGCt           >  2:1364630/1‑99 (MQ=255)
                                                                                             ccGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTcc        <  1:1105873/100‑1 (MQ=255)
                                                                                             ccGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTcc        <  1:1070181/100‑1 (MQ=255)
                                                                                             ccGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCACCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTcc        >  1:2520495/1‑100 (MQ=255)
                                                                                              cGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTccc       <  1:1007257/100‑1 (MQ=255)
                                                                                                 aTGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCt    <  1:440843/100‑1 (MQ=255)
                                                                                                  tgtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCTCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCtt   >  1:1989103/1‑100 (MQ=255)
                                                                                                   gtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg  <  1:2202209/100‑1 (MQ=255)
                                                                                                   gtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg  <  2:2505449/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTG  >  NC_000913/2028059‑2028257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: