Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,062,022 A→C 22.7% intergenic (‑89/‑238) nac ← / → asnV nitrogen assimilation regulon transcriptional regulator; autorepressor/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,062,0220AC22.7% 240.2 / 80.9 129intergenic (‑89/‑238)nac/asnVnitrogen assimilation regulon transcriptional regulator; autorepressor/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base A (43/56);  new base C (9/20);  total (52/77)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 132 total aligned reads displayed.

CTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGT  >  NC_000913/2061923‑2062120
                                                                                                   |                                                                                                  
cTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGa                                                                                                    <  2:2532056/100‑1 (MQ=255)
    aGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTa                                                                                                >  1:1495697/1‑100 (MQ=255)
    aGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTa                                                                                                <  1:347541/100‑1 (MQ=255)
      ttCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACa                                                                                              <  2:779606/100‑1 (MQ=255)
      ttCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACa                                                                                              <  2:2026634/100‑1 (MQ=255)
      ttCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACa                                                                                              <  1:934079/100‑1 (MQ=255)
       tCATGTTGCCTCCGGTTTTTAAGAATCGGTCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACaa                                                                                             <  1:2147763/100‑1 (MQ=255)
       tCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACaa                                                                                             <  2:113572/100‑1 (MQ=255)
        cATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAg                                                                                            <  1:418964/100‑1 (MQ=255)
        cATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAg                                                                                            <  1:65635/100‑1 (MQ=255)
         aTGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGc                                                                                           <  2:799809/100‑1 (MQ=255)
         aTGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGc                                                                                           <  1:1361184/100‑1 (MQ=255)
          tGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAg                                                                                            >  2:3046043/1‑98 (MQ=255)
          tGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCt                                                                                          >  2:778191/1‑100 (MQ=255)
            ttGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTaa                                                                                        >  2:1509190/1‑100 (MQ=255)
             tGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAAc                                                                                       >  1:2926058/1‑100 (MQ=255)
             tGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAAc                                                                                       <  2:1122108/100‑1 (MQ=255)
              gCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAAcc                                                                                      >  1:606735/1‑100 (MQ=255)
                cTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCaa                                                                                    >  2:103077/1‑100 (MQ=255)
                  ccGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACt                                                                                  >  1:520827/1‑100 (MQ=255)
                   cGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTg                                                                                 <  2:158657/100‑1 (MQ=255)
                   cGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTg                                                                                 <  2:2721356/100‑1 (MQ=255)
                   cGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTg                                                                                 >  1:2824884/1‑100 (MQ=255)
                     gTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTg                                                                                 <  1:798803/98‑1 (MQ=255)
                      tttttAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCa                                                                              <  2:3201139/100‑1 (MQ=255)
                       ttttAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCaa                                                                             <  2:1357583/100‑1 (MQ=255)
                        tttAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCaaa                                                                            <  2:1694232/100‑1 (MQ=255)
                         ttAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTTCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAAt                                                                           >  2:1512082/1‑100 (MQ=255)
                          tAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATa                                                                          <  1:2636159/100‑1 (MQ=255)
                          tAAGAATCGGCACAAGTGCCGCCATTACTTACAAACAGATTGCACGATGCTTGCCAGTTTTAATTTGATGATGATGTACTAACTAACCAAcctcaaa                                                                             >  1:3063564/1‑91 (MQ=255)
                             gAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAaga                                                                       <  1:1939607/100‑1 (MQ=255)
                              aaTCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAagag                                                                      <  1:404880/100‑1 (MQ=255)
                              aaTCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAagag                                                                      <  2:1741057/100‑1 (MQ=255)
                              aaTCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAagag                                                                      <  2:2804768/100‑1 (MQ=255)
                                tCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGAt                                                                    <  1:1884320/100‑1 (MQ=255)
                                  ggCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTa                                                                  >  2:2111425/1‑100 (MQ=255)
                                  ggCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTa                                                                  >  2:3284483/1‑100 (MQ=255)
                                  ggCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTa                                                                  >  2:3113995/1‑100 (MQ=255)
                                   gCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTAt                                                                 <  2:2931935/100‑1 (MQ=255)
                                        aGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAg                                                            >  2:3191369/1‑100 (MQ=255)
                                         gTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGa                                                           >  2:1454956/1‑100 (MQ=255)
                                           gccgccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGAtt                                                         >  1:1215238/1‑100 (MQ=255)
                                           gccgccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGAtt                                                         >  2:2801522/1‑100 (MQ=255)
                                           gccgccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGAtt                                                         >  2:2614175/1‑100 (MQ=255)
                                             cgccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCg                                                       <  1:2009505/100‑1 (MQ=255)
                                             cgccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCg                                                       <  2:3246934/100‑1 (MQ=255)
                                             cgccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCg                                                       <  1:1764660/100‑1 (MQ=255)
                                              gccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGt                                                      >  1:3152624/1‑100 (MQ=255)
                                               ccATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTc                                                     <  1:418744/100‑1 (MQ=255)
                                                  ttacttacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCAtt                                                  <  1:2446237/100‑1 (MQ=255)
                                                  ttacttacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCAtt                                                  <  1:2437472/100‑1 (MQ=255)
                                                    acttacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTg                                                <  2:3050669/100‑1 (MQ=255)
                                                    acttacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTg                                                >  2:1377539/1‑100 (MQ=255)
                                                     cttacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGc                                               >  1:1221828/1‑100 (MQ=255)
                                                     cttacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATCCGTCATTTGc                                               >  1:2229921/1‑100 (MQ=255)
                                                       tacAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTc                                             >  2:1623529/1‑100 (MQ=255)
                                                        acAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTcc                                            <  2:1810851/100‑1 (MQ=255)
                                                         cAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCt                                           >  2:36890/1‑100 (MQ=255)
                                                         cAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCt                                           >  1:2080184/1‑100 (MQ=255)
                                                           aCCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCttt                                         >  2:3203659/1‑100 (MQ=255)
                                                           aCCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCttt                                         >  2:2547959/1‑100 (MQ=255)
                                                           aCCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCttt                                         <  2:2431567/100‑1 (MQ=255)
                                                           aCCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCttt                                         <  1:2358000/100‑1 (MQ=255)
                                                            ccAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTa                                        >  1:2718612/1‑100 (MQ=255)
                                                            ccAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTa                                        <  1:3339657/100‑1 (MQ=255)
                                                              aGATTGCACGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACAAACTGTCAAATAAGAGATTATGATCGATTCGTCATTTGCTACTTTAAt                                      >  1:823220/1‑100 (MQ=255)
                                                               gATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATc                                     <  1:2801499/100‑1 (MQ=255)
                                                                  tGCAAGATGCTTGCCAGTTTTATTTTTGTGTTTATGTACAAGCTAACCAACTGTCAAATAAGCGATTATGATAGATTCGTCATTTGCTCCTTTAATCAg                                   >  1:2819112/1‑99 (MQ=255)
                                                                  tGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGc                                  <  1:105104/100‑1 (MQ=255)
                                                                  tGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGc                                  >  2:3003229/1‑100 (MQ=255)
                                                                   gCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCt                                 >  1:3186266/1‑100 (MQ=255)
                                                                    cAAGATGCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTg                                >  1:2524198/1‑100 (MQ=255)
                                                                     aaGATGCTTTCCAGTTTTTTTTTGGCGTTGTCGTACAAGCTATTTAAGTGTCAAATTAGAGATTATGATAGATTCGTCATTTGCTCCTTTAAtn                                     <  1:1444521/94‑2 (MQ=255)
                                                                       gATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTcg                             >  2:2022488/1‑100 (MQ=255)
                                                                       gATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTcg                             <  2:2557690/100‑1 (MQ=255)
                                                                         tGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTcgcg                           <  1:880062/100‑1 (MQ=255)
                                                                         tGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTcgcg                           <  2:2595582/100‑1 (MQ=255)
                                                                          gCTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGt                          <  1:2265833/100‑1 (MQ=255)
                                                                          gCTTGCCAGTTATATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGt                          >  1:1774757/1‑100 (MQ=255)
                                                                           cTTGCCAGTTTTATTTTGGTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGtt                         >  2:2351622/1‑100 (MQ=255)
                                                                           cTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCTCGtt                         >  1:1006566/1‑100 (MQ=255)
                                                                              gCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTccc                      <  2:520830/100‑1 (MQ=255)
                                                                                cAGTTTTATTTTGGTGTTGATGTACAAGCTAATGAACTGTCAAATAAGAGATGATGATAGATTCGTCATTTGCTCCGTTAATCAGCTGTCGCGTTcccc                     <  1:1532166/99‑1 (MQ=255)
                                                                                  gtgttATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGc                  <  1:2602648/97‑1 (MQ=255)
                                                                                  gTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGc                  >  2:351314/1‑100 (MQ=255)
                                                                                  gTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGc                  >  2:635610/1‑100 (MQ=255)
                                                                                    tttATTTTGGTGTTGATGTACAAGCTATCCAACTGTCACATAAGATATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGccc                <  1:134960/100‑1 (MQ=255)
                                                                                    tttATTTTGGTGTTGATGTACAAGCTAACCTACTGTCAAATAAGAGATGATGACAGATTCGTCATGTCCTCCTTTAAACAGCCGTCGCGTTCTCCTGccc                <  1:1309699/100‑1 (MQ=255)
                                                                                    tttATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGccc                <  2:2375648/100‑1 (MQ=255)
                                                                                    tttATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGccc                <  1:3203631/100‑1 (MQ=255)
                                                                                    ttcaTTTTCGTGTTGATGTACAAGCTGACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTg                   <  1:3364732/94‑1 (MQ=255)
                                                                                            ggTGTTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAag        <  2:2583256/100‑1 (MQ=255)
                                                                                               gtTGCTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATaaa          <  2:1032443/95‑1 (MQ=255)
                                                                                               gtTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAaggag     <  1:1793617/100‑1 (MQ=255)
                                                                                               gtTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAaggag     <  2:1597069/100‑1 (MQ=255)
                                                                                                 tGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAggg   <  2:508930/100‑1 (MQ=255)
                                                                                                 tGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAggg   <  2:354495/100‑1 (MQ=255)
                                                                                                 tGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAggg   <  1:2933409/100‑1 (MQ=255)
                                                                                                  gATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGt  <  1:2705982/100‑1 (MQ=255)
                                                                                                   aTGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATGCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGt  <  1:1931869/99‑1 (MQ=255)
                                                                                                   |                                                                                                  
CTGAAGTTCATGTTGCCTCCGGTTTTTAAGAATCGGCCCAAGTGCCGCCATTACTTACAACCAGATTGCAAGATGCTTGCCAGTTTTATTTTGGTGTTGATGTACAAGCTAACCAACTGTCAAATAAGAGATTATGATAGATTCGTCATTTGCTCCTTTAATCAGCTGTCGCGTTCCCCTGCCCTATAAAAGGAGGGT  >  NC_000913/2061923‑2062120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: