Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,140,400 G→A 15.1% R405W (CGG→TGG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,4000GA15.1% 305.7 / 35.7 123R405W (CGG→TGG) asmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (50/53);  new base A (10/8);  total (62/61)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 127 total aligned reads displayed.

CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTC  >  NC_000913/2140301‑2140499
                                                                                                   |                                                                                                   
cAGCACGGGAGAAATCACCGGCCAGTGACATTTTTCCGTTCAACGAAATCGGAGAGTAAAACGGCTTCATGATGGTACCGATCTCAATTTTTTCCAGCCg                                                                                                     >  1:2374665/1‑100 (MQ=255)
cAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCg                                                                                                     >  2:727938/1‑100 (MQ=255)
  gCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTg                                                                                                   >  2:2550806/1‑100 (MQ=255)
   cACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAAAGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCCTgg                                                                                                  >  1:34323/1‑100 (MQ=255)
      cGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCGCAACGTTTTCCAGCCGTGGCTg                                                                                               >  1:2520220/1‑100 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                           <  2:1889914/100‑1 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                           <  1:1179075/100‑1 (MQ=255)
          gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                           >  1:1598205/1‑100 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGt                                                                                          <  2:1142311/100‑1 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGt                                                                                          <  2:617736/100‑1 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGtt                                                                                         >  1:1572975/1‑100 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGtt                                                                                         <  2:1808026/100‑1 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  2:46421/100‑4 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  2:86850/100‑4 (MQ=255)
             aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGttt                                                                                        <  2:1618753/100‑1 (MQ=255)
             aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacg                                                                                        <  1:109964/100‑5 (MQ=255)
              tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                                                       <  2:1887450/100‑6 (MQ=255)
               cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCATGGCTGGAAGTTTAt                                                                                      >  2:3235663/1‑100 (MQ=255)
                aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATc                                                                                     >  2:570762/1‑100 (MQ=255)
                 ccagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATcc                                                                                    >  2:935864/1‑100 (MQ=255)
                 ccagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgttga                                                                                    <  2:2376408/100‑9 (MQ=39)
                  cagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgttgag                                                                                   <  2:1257431/100‑10 (MQ=38)
                     ccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgc                                                                                  >  1:74593/1‑98 (MQ=255)
                       agTGACATGTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGAt                                                                              <  1:101510/100‑1 (MQ=255)
                          gACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTa                                                                           <  1:807709/100‑1 (MQ=255)
                           aCATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAt                                                                          <  1:2210076/100‑1 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         <  2:2783089/100‑1 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         <  2:1406758/100‑1 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGACAGTTAAACGCCTTCCGAATGGTACCAACCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         >  1:1907323/1‑100 (MQ=255)
                                tttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATg                                                                     <  1:602840/100‑1 (MQ=255)
                                   ccGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcg                                                                  <  2:2134064/100‑1 (MQ=255)
                                    cGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgc                                                                 >  2:1157332/1‑100 (MQ=255)
                                     ggTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                                >  2:581711/1‑100 (MQ=255)
                                     ggTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                                <  2:1536555/100‑1 (MQ=255)
                                      gTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGt                                                               >  2:717609/1‑100 (MQ=255)
                                       tCAACGAAATCGGATAGTTAAACGCCTACAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCGGGAAGTTTATCCGCGGATTTATTGATGTCGCGt                                                               >  2:306838/1‑99 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                             >  1:44496/1‑100 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                             >  2:1630381/1‑100 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                             >  2:2637634/1‑100 (MQ=255)
                                          aCGAAGTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgc                                                                 >  1:3112320/1‑94 (MQ=255)
                                          aCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTGGCGTCCAg                                                           >  1:2874393/1‑100 (MQ=255)
                                            gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                         <  2:228248/100‑1 (MQ=255)
                                            gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                         <  1:1017652/100‑1 (MQ=255)
                                            gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                         >  1:1884184/1‑100 (MQ=255)
                                              aaTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTg                                                       <  2:469262/100‑1 (MQ=255)
                                               aTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGc                                                      >  2:2078097/1‑100 (MQ=255)
                                               aTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGc                                                      >  2:2990158/1‑100 (MQ=255)
                                                 cGGATAGTTAAACGCCTTCAGAAAGGTACCAATCTCAACGTTTTCCAGCCGTGTCTGGAAGTTTATCCGCGGATTTATTAATGTCGCGTCCAGCGTGccc                                                    >  1:592042/1‑100 (MQ=255)
                                                  ggATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                   >  2:103856/1‑100 (MQ=255)
                                                  ggATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                   <  2:2584209/100‑1 (MQ=255)
                                                    aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGc                                                 <  1:693859/100‑1 (MQ=255)
                                                     tAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCa                                                >  2:890581/1‑100 (MQ=255)
                                                     tAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCa                                                <  2:446264/100‑1 (MQ=255)
                                                      aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                               <  2:1923190/100‑1 (MQ=255)
                                                      aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                               <  1:2467834/100‑1 (MQ=255)
                                                       gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                              <  2:1729108/100‑1 (MQ=255)
                                                        ttAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTg                                             <  2:381068/100‑1 (MQ=255)
                                                          aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                           >  2:3111840/1‑100 (MQ=255)
                                                          aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                           >  2:250418/1‑100 (MQ=255)
                                                            aCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                         <  1:2473359/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        <  1:2246726/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        <  1:1116173/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        <  2:807309/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        >  2:2557644/1‑100 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        <  2:1579409/100‑1 (MQ=255)
                                                              gCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCACGTCCAGCGTGCCCGGCAGTGAAACCt                                       <  2:2815405/100‑1 (MQ=255)
                                                               ccTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTg                                      >  2:3120079/1‑100 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     >  1:2795979/1‑100 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     >  1:1497010/1‑100 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     <  2:3193726/100‑1 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     <  2:3100736/100‑1 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     <  1:3132198/100‑1 (MQ=255)
                                                                           tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAg                          >  1:2314140/1‑100 (MQ=255)
                                                                           tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAg                          >  2:1317977/1‑100 (MQ=255)
                                                                            aCCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGt                         <  2:2259761/100‑1 (MQ=255)
                                                                            aCCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGt                         >  2:528976/1‑100 (MQ=255)
                                                                             ccAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGTAACCTGTCCACCGTTAAGtt                        <  1:2068262/100‑1 (MQ=255)
                                                                             ccAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGtt                        >  1:2820471/1‑100 (MQ=255)
                                                                                 tctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGcc                    <  2:383999/100‑1 (MQ=255)
                                                                                  ctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGccc                   >  1:3215635/1‑100 (MQ=255)
                                                                                   tcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCt                  >  2:596414/1‑100 (MQ=255)
                                                                                   tcAACGTTTTCCAGCCGCGGCTGGAAGGTTATCCGCGGATTTATTGCTGTCGCGTCAAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTCTGCCCt                  >  1:1368342/1‑100 (MQ=255)
                                                                                    gataaacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTg                 >  2:1332261/8‑100 (MQ=255)
                                                                                    cAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTg                 >  2:3366010/1‑100 (MQ=255)
                                                                                      aCGTTTTCCAGCCGTGGCTGGAAGTTTAGCCGCGGATTTATTGATGTCGCGTGCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc                <  1:3127362/99‑1 (MQ=255)
                                                                                        gTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGTAACCTGTCCACCGTTAAGTTTGCCCGGCAGt             >  1:730108/1‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAg              >  2:3018342/4‑99 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  2:379974/4‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  1:1556579/4‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  2:2912951/4‑100 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt            >  1:1358841/3‑100 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt            >  1:536175/3‑100 (MQ=255)
                                                                                          tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg           >  1:68461/1‑100 (MQ=255)
                                                                                          tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg           >  1:1337174/1‑100 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  2:1098519/100‑1 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  1:61235/100‑1 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  1:2550786/100‑1 (MQ=255)
                                                                                             ccAGCCGTGGCTGGAAGTTTATCCGCGGATTTGTTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTGAAGTTTGCCCTGCAGTTGAGt        >  2:3217373/1‑100 (MQ=255)
                                                                                              cAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTa       <  2:1791168/100‑1 (MQ=255)
                                                                                                   gTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTc  >  2:2936559/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTC  >  NC_000913/2140301‑2140499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: