Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,140,401 T→C 15.2% P404P (CCA→CCG asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,4010TC15.2% 290.7 / 41.1 125P404P (CCA→CCGasmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (52/54);  new base C (11/8);  total (63/62)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 125 total aligned reads displayed.

GCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTC  >  NC_000913/2140303‑2140499
                                                                                                  |                                                                                                  
gCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTg                                                                                                   >  2:2550806/1‑100 (MQ=255)
 cACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAAAGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCCTgg                                                                                                  >  1:34323/1‑100 (MQ=255)
    cGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCGCAACGTTTTCCAGCCGTGGCTg                                                                                               >  1:2520220/1‑100 (MQ=255)
     ggAGAAATCACCAGCCAGTGATATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTgg                                                                                              <  1:337463/100‑1 (MQ=255)
        gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                           <  2:1889914/100‑1 (MQ=255)
        gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                           <  1:1179075/100‑1 (MQ=255)
        gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                           <  2:2904186/100‑1 (MQ=255)
        gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                                                           >  1:1598205/1‑100 (MQ=255)
         aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGt                                                                                          <  2:1142311/100‑1 (MQ=255)
         aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGt                                                                                          <  2:617736/100‑1 (MQ=255)
          aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGtt                                                                                         >  1:1572975/1‑100 (MQ=255)
          aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGtt                                                                                         <  2:1226684/100‑1 (MQ=255)
          aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGtt                                                                                         <  2:1808026/100‑1 (MQ=255)
          aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  2:86850/100‑4 (MQ=255)
          aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  2:1496278/100‑4 (MQ=255)
           aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGttt                                                                                        <  2:1618753/100‑1 (MQ=255)
           aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacg                                                                                        <  1:109964/100‑5 (MQ=255)
            tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                                                       <  2:1887450/100‑6 (MQ=255)
             cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCATGGCTGGAAGTTTAt                                                                                      >  2:3235663/1‑100 (MQ=255)
              aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATc                                                                                     >  2:570762/1‑100 (MQ=255)
               ccagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATcc                                                                                    >  2:935864/1‑100 (MQ=255)
                cagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgttgag                                                                                   <  2:1257431/100‑10 (MQ=38)
                   ccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgc                                                                                  >  1:74593/1‑98 (MQ=255)
                     agTGACATGTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGAt                                                                              <  1:101510/100‑1 (MQ=255)
                          cATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         <  2:2783089/100‑1 (MQ=255)
                          cATTTTTCCGGTCAACGAAATCGGACAGTTAAACGCCTTCCGAATGGTACCAACCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         >  1:1907323/1‑100 (MQ=255)
                              tttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATg                                                                     <  1:602840/100‑1 (MQ=255)
                                 ccGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcg                                                                  <  2:2134064/100‑1 (MQ=255)
                                   ggTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                                <  2:1536555/100‑1 (MQ=255)
                                    gTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGt                                                               <  1:598287/100‑1 (MQ=255)
                                    gTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGt                                                               >  2:717609/1‑100 (MQ=255)
                                      cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                             >  2:1630381/1‑100 (MQ=255)
                                      cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                             >  2:2637634/1‑100 (MQ=255)
                                      cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                             >  1:44496/1‑100 (MQ=255)
                                      cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                             <  1:6979/100‑1 (MQ=255)
                                        aCGAAGTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgc                                                                 >  1:3112320/1‑94 (MQ=255)
                                        aCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTGGCGTCCAg                                                           >  1:2874393/1‑100 (MQ=255)
                                          gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                         <  2:228248/100‑1 (MQ=255)
                                          gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                         <  1:1017652/100‑1 (MQ=255)
                                          gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                         >  1:1884184/1‑100 (MQ=255)
                                            aaTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTg                                                       <  2:469262/100‑1 (MQ=255)
                                             aTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGc                                                      >  2:2990158/1‑100 (MQ=255)
                                             aTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGc                                                      >  2:2078097/1‑100 (MQ=255)
                                             aTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTGTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGc                                                      <  2:847162/100‑1 (MQ=255)
                                               cGGATAGTTAAACGCCTTCAGAAAGGTACCAATCTCAACGTTTTCCAGCCGTGTCTGGAAGTTTATCCGCGGATTTATTAATGTCGCGTCCAGCGTGccc                                                    >  1:592042/1‑100 (MQ=255)
                                                ggATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                   >  2:103856/1‑100 (MQ=255)
                                                  aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGc                                                 <  1:693859/100‑1 (MQ=255)
                                                   tAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCa                                                >  2:890581/1‑100 (MQ=255)
                                                   tAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCa                                                <  2:446264/100‑1 (MQ=255)
                                                    aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                               <  2:1923190/100‑1 (MQ=255)
                                                    aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                               <  1:2467834/100‑1 (MQ=255)
                                                     gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                              >  2:602904/1‑100 (MQ=255)
                                                     gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                              >  1:2602358/1‑100 (MQ=255)
                                                     gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                              <  2:1729108/100‑1 (MQ=255)
                                                      ttAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTg                                             <  2:381068/100‑1 (MQ=255)
                                                        aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                           >  2:3111840/1‑100 (MQ=255)
                                                        aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                           >  2:250418/1‑100 (MQ=255)
                                                         aaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaaa                                          >  2:2327979/1‑100 (MQ=255)
                                                           cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        <  1:1116173/100‑1 (MQ=255)
                                                           cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        >  2:2557644/1‑100 (MQ=255)
                                                           cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        <  1:2246726/100‑1 (MQ=255)
                                                           cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                        <  2:1579409/100‑1 (MQ=255)
                                                            gCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCACGTCCAGCGTGCCCGGCAGTGAAACCt                                       <  2:2815405/100‑1 (MQ=255)
                                                             ccTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTg                                      >  2:3120079/1‑100 (MQ=255)
                                                              cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     >  1:1497010/1‑100 (MQ=255)
                                                              cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     <  2:3100736/100‑1 (MQ=255)
                                                              cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                     >  1:2795979/1‑100 (MQ=255)
                                                                         tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGtt                             >  1:2171332/1‑97 (MQ=255)
                                                                         tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAg                          >  1:2314140/1‑100 (MQ=255)
                                                                         tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAg                          >  2:1317977/1‑100 (MQ=255)
                                                                          aCCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGt                         <  2:2259761/100‑1 (MQ=255)
                                                                          aCCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGt                         >  2:528976/1‑100 (MQ=255)
                                                                           ccAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGTAACCTGTCCACCGTTAAGtt                        <  1:2068262/100‑1 (MQ=255)
                                                                             aaTCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGttta                      <  2:500922/100‑2 (MQ=255)
                                                                               tctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGcc                    <  2:2567021/100‑1 (MQ=255)
                                                                               tctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGcc                    <  2:383999/100‑1 (MQ=255)
                                                                                ctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGccc                   >  1:3215635/1‑100 (MQ=255)
                                                                                 tcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCt                  >  2:596414/1‑100 (MQ=255)
                                                                                 tcAACGTTTTCCAGCCGCGGCTGGAAGGTTATCCGCGGATTTATTGCTGTCGCGTCAAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTCTGCCCt                  >  1:1368342/1‑100 (MQ=255)
                                                                                  gataaacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTg                 >  2:1332261/8‑100 (MQ=255)
                                                                                  cAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTg                 >  2:3366010/1‑100 (MQ=255)
                                                                                    aCGTTTTCCAGCCGTGGCTGGAAGTTTAGCCGCGGATTTATTGATGTCGCGTGCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc                <  1:3127362/99‑1 (MQ=255)
                                                                                     cGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAg              <  2:3266154/100‑1 (MQ=255)
                                                                                     cGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAg              >  2:3115350/1‑100 (MQ=255)
                                                                                      gTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGTAACCTGTCCACCGTTAAGTTTGCCCGGCAGt             >  1:730108/1‑100 (MQ=255)
                                                                                      aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAg              >  2:3018342/4‑99 (MQ=255)
                                                                                      aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  1:1556579/4‑100 (MQ=255)
                                                                                       acttCCAGCCCCGGCTGGAAGTTTATCCGCGGATTTATTGATGTGGCGTCCAGCGTGACCGGCAGTGAAACGTGTCCACCGTTAAGTTTGCCCTGCAGt             >  1:23803/3‑99 (MQ=255)
                                                                                       acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt            >  1:1358841/3‑100 (MQ=255)
                                                                                        tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg           >  1:1337174/1‑100 (MQ=255)
                                                                                        cttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg           >  2:3029550/2‑100 (MQ=255)
                                                                                          tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  2:44496/100‑1 (MQ=255)
                                                                                          tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  2:1098519/100‑1 (MQ=255)
                                                                                          tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  1:61235/100‑1 (MQ=255)
                                                                                          tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  1:2550786/100‑1 (MQ=255)
                                                                                           ccAGCCGTGGCTGGAAGTTTATCCGCGGATTTGTTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTGAAGTTTGCCCTGCAGTTGAGt        >  2:3217373/1‑100 (MQ=255)
                                                                                            cAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTa       <  2:1791168/100‑1 (MQ=255)
                                                                                              gCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  1:953236/96‑1 (MQ=255)
                                                                                                 gTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTc  <  1:2968108/100‑1 (MQ=255)
                                                                                                 gTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTc  >  2:2936559/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
GCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTC  >  NC_000913/2140303‑2140499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: